FastQCFastQC Report
Fri 10 Feb 2017
SRR3224477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924727
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG30090.3253933323023984No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA22260.2407196934879159No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA20160.21801028844188608No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA17340.18751480166578893No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA16360.17691707931097503No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC15030.16253445611515616No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG14540.1572355949377492No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA13940.1507471934960264No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA13460.14555647234264815No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC13360.14447507210236102No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC13230.14306925178998775No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT13180.14252855166984418No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA11890.12857848857014018No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG11880.12847034854611145No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC11600.12544242787330748No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA11310.12230636717647479No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC10960.11852146633546982No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC10050.1086807241488569No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA9960.10770746393259849No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA9950.10759932390856977No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG9730.10522024337993809No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG9510.10284116285130637No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT9320.10078650239476084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.053.4401441
GTATCAA15350.045.2142221
AAACCGT1651.8189894E-1234.5292746
ATCAACG19450.033.6860123
AACCGTG1753.6379788E-1232.556177
ATGCGGG2400.031.6535458
TCAACGC21550.030.4033874
AATGCGG2500.030.385767
TCGCACC11450.029.8921811
CGCACCA11650.029.3424742
CCGTGCG1802.0918378E-1029.036169
GTTCCGG8350.028.9983866
GTACATG49100.028.9481141
TCCGGGC8700.028.9247918
CTATTAA4550.028.208691
ATTAACC4050.028.1349623
TTCCGGG8800.028.0550357
TGTTCCG8650.027.992665
CATGGGA16050.027.8062844
ACATGGG49650.027.635593
Produced by FastQC (version 0.11.5)