##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224477_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	924727
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.263278783900546	34.0	31.0	34.0	31.0	34.0
2	32.424802130791036	34.0	31.0	34.0	31.0	34.0
3	32.56299751169805	34.0	31.0	34.0	31.0	34.0
4	35.85219097095683	37.0	37.0	37.0	35.0	37.0
5	35.87007733093118	37.0	37.0	37.0	35.0	37.0
6	35.975424098139236	37.0	37.0	37.0	35.0	37.0
7	35.937623752739995	37.0	37.0	37.0	35.0	37.0
8	35.975452214545484	37.0	37.0	37.0	35.0	37.0
9	37.71297474822299	39.0	39.0	39.0	35.0	39.0
10-11	37.7875724402986	39.0	39.0	39.0	36.0	39.0
12-13	37.78071257787434	39.0	39.0	39.0	35.0	39.0
14-15	39.27455886980698	41.0	40.0	41.0	37.0	41.0
16-17	39.11263107922663	41.0	40.0	41.0	36.5	41.0
18-19	39.203817991688354	41.0	40.0	41.0	36.5	41.0
20-21	39.273659685507184	41.0	40.0	41.0	37.0	41.0
22-23	39.225830974979644	41.0	40.0	41.0	36.0	41.0
24-25	39.17178745727117	41.0	40.0	41.0	36.0	41.0
26-27	39.01045984382418	41.0	39.0	41.0	35.5	41.0
28-29	38.9455450095001	41.0	39.0	41.0	35.0	41.0
30-31	38.883047645413185	41.0	39.0	41.0	35.0	41.0
32-33	38.697580475102384	41.0	39.0	41.0	35.0	41.0
34-35	38.705342225327044	41.0	39.0	41.0	35.0	41.0
36-37	38.703630368746666	41.0	39.0	41.0	35.0	41.0
38-39	38.57933855072903	40.5	39.0	41.0	35.0	41.0
40-41	38.48839224982076	40.0	39.0	41.0	35.0	41.0
42-43	38.35727787768714	40.0	38.0	41.0	34.0	41.0
44-45	38.18410028040708	40.0	38.0	41.0	34.0	41.0
46-47	38.0198766771166	40.0	38.0	41.0	33.0	41.0
48-49	37.817526686254425	40.0	37.0	41.0	33.0	41.0
50-51	36.54227626099379	38.5	35.5	40.0	31.5	40.5
52-53	36.84909438136877	39.0	36.0	40.0	32.0	40.5
54-55	37.29836805889738	40.0	36.0	41.0	33.0	41.0
56-57	37.16406193395456	39.5	35.0	41.0	33.0	41.0
58-59	37.03139250827542	39.0	35.0	41.0	33.0	41.0
60-61	36.804864570840905	39.0	35.0	41.0	32.0	41.0
62-63	36.56396914981394	39.0	35.0	41.0	32.0	41.0
64-65	36.34584044804575	38.0	35.0	41.0	31.0	41.0
66-67	36.15368211374817	37.0	35.0	41.0	31.0	41.0
68-69	35.89508795568854	37.0	35.0	40.0	31.0	41.0
70-71	35.64139254071742	36.0	35.0	40.0	31.0	41.0
72-73	35.343546257435975	36.0	35.0	39.0	30.5	41.0
74-75	35.07483938502931	35.5	35.0	39.0	30.0	41.0
76-77	34.746671720410454	35.0	35.0	38.5	30.0	40.0
78-79	34.49484983135563	35.0	34.0	37.0	30.0	39.0
80-81	34.164821076923246	35.0	34.0	37.0	29.5	39.0
82-83	33.86418099612102	35.0	34.0	36.5	29.0	39.0
84-85	33.59514105244034	35.0	34.0	36.0	29.0	37.0
86-87	33.34692184828604	35.0	34.0	36.0	29.0	37.0
88-89	33.179967709388826	35.0	34.0	35.0	29.0	36.5
90-91	32.97840119300075	35.0	34.0	35.0	28.5	36.0
92-93	32.822523296064674	35.0	34.0	35.0	27.5	36.0
94-95	32.67185937038715	35.0	33.5	35.0	27.0	36.0
96-97	32.524422883726764	35.0	33.0	35.0	27.0	35.5
98-99	32.46836688017112	35.0	33.0	35.0	27.0	35.0
100-101	31.25022790510064	34.5	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	288.0
8	1226.0
9	838.0
10	710.0
11	941.0
12	1308.0
13	1501.0
14	1603.0
15	1666.0
16	1767.0
17	1918.0
18	2100.0
19	2230.0
20	2844.0
21	3652.0
22	4354.0
23	4285.0
24	4250.0
25	4498.0
26	4694.0
27	4893.0
28	5335.0
29	6216.0
30	7635.0
31	9657.0
32	13347.0
33	19247.0
34	32665.0
35	59615.0
36	128809.0
37	205460.0
38	281840.0
39	103268.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23276366235623	20.596096779084625	13.367448610479347	23.803690948079797
2	15.517552748000222	20.744176389355996	39.050660357056735	24.687610505587053
3	19.209669448388553	28.700146097172464	28.350313119439573	23.73987133499941
4	12.518289181563858	17.330303970793544	40.353098806458554	29.798308041184047
5	12.812754466994042	40.146335080515655	34.417725447618594	12.62318500487171
6	27.501846490910292	38.97128557941966	20.16541098075432	13.361456948915734
7	26.095377338392844	31.36644652962442	23.946851340990367	18.59132479099237
8	25.296655120916768	35.34470173359273	21.350950064181102	18.0076930813094
9	26.867280829909802	15.628396272629653	20.935908651958904	36.56841424550164
10-11	25.398306743503756	25.877691470023045	28.14771278442178	20.576289002051414
12-13	25.8078330145005	23.745710896296963	28.37632079521848	22.070135293984062
14-15	23.17851600595203	25.62185569843048	25.458667089287484	25.74096120633001
16-17	22.550635459322812	28.499192689620546	26.795670392270598	22.154501458786047
18-19	22.53135311210972	27.826052625743703	27.37002702514144	22.272567237005138
20-21	23.77549828208435	26.22309536379136	27.04689433736921	22.954512016755086
22-23	23.819865677835654	27.160173202333244	26.2238785700798	22.796082549751304
24-25	23.122773962786987	27.128261925286267	26.59828525507561	23.15067885685114
26-27	22.897839037899832	28.08245027992045	27.060310772801056	21.95939990937866
28-29	22.385255324003733	27.9361368274096	27.405385589476676	22.273222259109986
30-31	22.65106350306631	27.160772855123728	27.31849508016961	22.869668561640356
32-33	22.80592001747543	27.076153286321258	27.57159680640881	22.5463298897945
34-35	23.324289222657065	26.746812843141814	27.062689853329687	22.866208080871438
36-37	22.40327903251451	26.77636794906878	27.831053463286104	22.9892995551306
38-39	22.712095544042906	27.43041267633736	26.858919835814092	22.998571943805636
40-41	23.168942685422756	26.53871214558589	27.7715744137821	22.52077075520925
42-43	22.91784385471341	27.006915241919916	27.619332397170314	22.455908506196355
44-45	22.949408384655694	26.507644413248766	27.374261798915136	23.168685403180408
46-47	23.12877205921315	26.543185177895744	26.985153456101095	23.342889306790006
48-49	22.340269074007786	26.878473322396772	27.453345690133414	23.327911913462028
50-51	22.444137567087367	27.29443392482322	27.09448302039413	23.166945487695287
52-53	22.660363545132782	26.940059066081123	26.824295170358386	23.57528221842771
54-55	22.871074381952727	26.60093195072708	26.990181967218437	23.53781170010176
56-57	22.879995933935096	26.814292218135733	26.9230270122966	23.38268483563257
58-59	22.934868428168162	26.79171308363707	27.426697238776104	22.84672124941866
60-61	22.70894983213927	27.3421005952999	27.16147726092825	22.787472311632577
62-63	22.2687114474008	26.6897430142217	27.93786693420807	23.103678604169428
64-65	23.25021570552612	26.203014738299835	26.91610069323293	23.630668862941114
66-67	22.325561272912616	27.07289044396194	26.48937752312555	24.11217075999989
68-69	22.517186153318764	26.835866152929462	27.930621686184136	22.716326007567638
70-71	23.061022334159162	26.523665903558562	27.338338774578872	23.0769729877034
72-73	22.561036933062407	26.91989095159977	27.558944423597453	22.96012769174037
74-75	22.853014997939933	26.3656192584406	27.485895837366055	23.295469906253413
76-77	22.513022762393657	26.071208043022427	27.31768402998939	24.098085164594522
78-79	22.83844395869679	25.46023749238841	27.559575892543293	24.14174265637151
80-81	22.418201432387892	26.90033770075666	26.78342463841349	23.898036228441956
82-83	22.172405641367142	27.15834333662058	27.31154374840078	23.3577072736115
84-85	22.518453936551342	26.020050537392482	27.857439554163278	23.6040559718929
86-87	22.223105875555284	27.35184785437249	27.465298122092396	22.959748147979834
88-89	22.71536586328894	26.278505359367248	26.878780453900568	24.127348323443236
90-91	22.42283398235371	26.19037834950207	27.10313422231642	24.283653445827795
92-93	22.35723385249607	26.229704096706584	27.77243066354924	23.640631387248106
94-95	22.639168100423152	25.64913752923836	27.541587949740844	24.170106420597648
96-97	23.0675648056129	26.291813692041003	27.573002626721184	23.067618875624916
98-99	21.682119083550113	26.298162525865926	27.852524087007914	24.167194303576053
100-101	22.57937654238843	25.91515497724216	27.402609767206858	24.102858713162544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	2.5
4	3.0
5	2.5
6	3.0
7	6.0
8	11.0
9	15.0
10	17.5
11	18.0
12	20.5
13	22.5
14	17.5
15	23.0
16	36.0
17	32.5
18	35.5
19	43.5
20	47.5
21	57.0
22	73.5
23	90.5
24	122.5
25	169.0
26	221.5
27	278.5
28	366.5
29	550.0
30	1092.5
31	2240.0
32	4520.5
33	8453.0
34	13895.0
35	21888.0
36	34319.5
37	45354.5
38	50327.0
39	52553.5
40	50747.0
41	45818.5
42	40194.5
43	34433.5
44	30467.5
45	28893.0
46	28439.0
47	26062.0
48	25106.5
49	27507.0
50	30707.0
51	30804.0
52	29966.5
53	31387.5
54	33806.5
55	34507.5
56	28313.5
57	23108.0
58	22167.5
59	21482.0
60	18340.5
61	13665.5
62	11053.0
63	7690.5
64	4132.5
65	2259.0
66	1751.0
67	1738.5
68	1332.0
69	744.5
70	368.0
71	194.0
72	137.5
73	120.0
74	88.0
75	57.0
76	43.0
77	33.5
78	23.5
79	17.0
80	12.5
81	9.5
82	5.5
83	4.0
84	7.5
85	9.5
86	8.5
87	5.0
88	2.0
89	4.0
90	4.5
91	2.5
92	1.5
93	1.5
94	2.0
95	1.0
96	0.5
97	2.5
98	3.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14999021332782542
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03638911808566204
16-17	0.07256195612326666
18-19	0.02352045522624515
20-21	0.03795714843407838
22-23	0.06547878454938592
24-25	0.017086123796536706
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.020114044469340682
38-39	0.004704091045249031
40-41	0.00535293118942131
42-43	0.003298270732875757
44-45	0.010543652342799551
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.014815183291933728
60-61	0.016329143628335714
62-63	0.02806233623545111
64-65	0.04698684044047595
66-67	0.01670763371243621
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016221003604307
80-81	0.057963052879390346
82-83	0.04839266075284922
84-85	0.02844082631955161
86-87	0.05769270281931857
88-89	0.03433445762911649
90-91	0.0
92-93	0.00356862079294754
94-95	0.0
96-97	0.0
98-99	7.029101561866367E-4
100-101	0.046500210332346734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	924727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.380639698160756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.22095135504568	23.454799484035775
2	15.17621611133088	10.435360362101786
3	6.008539735185638	6.19732319242499
4	3.1206646468242774	4.291617873650143
5	1.7878766469716798	3.073417141214454
6	1.1609041606135113	2.3947576602089327
7	0.7773495677836063	1.8708042786642411
8	0.5704601444229381	1.5690227750052628
9	0.42350894287794527	1.3104457536632024
>10	2.244438758182958	14.37892609419175
>50	0.239326810407177	5.838502615048862
>100	0.24092858254874486	16.78090944786126
>500	0.02306763024399974	5.4230141575494
>1k	0.005766907560999935	2.9810991643799274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	3009	0.3253933323023984	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	2226	0.2407196934879159	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	2016	0.21801028844188608	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1734	0.18751480166578893	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1636	0.17691707931097503	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1503	0.16253445611515616	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	1454	0.1572355949377492	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1394	0.1507471934960264	No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA	1346	0.14555647234264815	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1336	0.14447507210236102	No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC	1323	0.14306925178998775	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1318	0.14252855166984418	No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA	1189	0.12857848857014018	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1188	0.12847034854611145	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	1160	0.12544242787330748	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	1131	0.12230636717647479	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1096	0.11852146633546982	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	1005	0.1086807241488569	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	996	0.10770746393259849	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	995	0.10759932390856977	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	973	0.10522024337993809	No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG	951	0.10284116285130637	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	932	0.10078650239476084	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.0814002402871334E-4	0.0
12-13	0.0	0.0	0.0	1.0814002402871334E-4	0.0
14-15	0.0	0.0	0.0	1.0814002402871334E-4	0.0
16-17	0.0	0.0	0.0	3.2442007208614E-4	0.0
18-19	0.0	0.0	0.0	3.2442007208614E-4	0.0
20-21	0.0	0.0	0.0	3.784900841004967E-4	0.0
22-23	0.0	0.0	0.0	5.407001201435667E-4	0.0
24-25	0.0	0.0	0.0	0.00129768028834456	0.0
26-27	0.0	0.0	0.0	0.0021628004805742665	0.0
28-29	0.0	0.0	0.0	0.004109320913091107	0.0
30-31	0.0	0.0	0.0	0.010165162258699054	0.0
32-33	0.0	0.0	0.0	0.027846056187393685	0.0
34-35	0.0	0.0	0.0	0.052123491581839834	0.0
36-37	0.0	0.0	0.0	0.09208123046044941	0.0
38-39	0.0	0.0	0.0	0.16415655647558686	0.0
40-41	0.0	0.0	0.0	0.25077671572258625	0.0
42-43	0.0	0.0	0.0	0.3308544035158485	0.0
44-45	0.0	0.0	0.0	0.42244900386816864	0.0
46-47	0.0	0.0	0.0	0.5375099894347197	0.0
48-49	0.0	0.0	0.0	0.6546256354578162	0.0
50-51	0.0	0.0	0.0	0.7829337739678845	0.0
52-53	0.0	0.0	0.0	0.9149186732949293	0.0
54-55	0.0	0.0	0.0	1.0465791525498878	0.0
56-57	0.0	0.0	0.0	1.2010031068628904	0.0
58-59	0.0	0.0	0.0	1.371485854744157	0.0
60-61	0.0	0.0	0.0	1.5578651861576445	0.0
62-63	0.0	0.0	0.0	1.7591678408870943	0.0
64-65	0.0	0.0	0.0	1.958145485099927	0.0
66-67	0.0	0.0	0.0	2.1812383546711622	0.0
68-69	0.0	0.0	0.0	2.447100603745754	0.0
70-71	0.0	0.0	0.0	2.7364292380345767	0.0
72-73	0.0	0.0	0.0	3.0328951139092943	0.0
74-75	0.0	0.0	0.0	3.3370389314900506	0.0
76-77	0.0	0.0	0.0	3.65102349125742	0.0
78-79	0.0	0.0	0.0	4.01329257175361	0.0
80-81	0.0	0.0	0.0	4.402650728268991	0.0
82-83	0.0	0.0	0.0	4.814880499866447	0.0
84-85	0.0	0.0	0.0	5.234247513049797	0.0
86-87	0.0	0.0	0.0	5.674593690894718	0.0
88-89	0.0	0.0	0.0	6.166144170117235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	765	0.0	53.440144	1
GTATCAA	1535	0.0	45.214222	1
AAACCGT	165	1.8189894E-12	34.529274	6
ATCAACG	1945	0.0	33.686012	3
AACCGTG	175	3.6379788E-12	32.55617	7
ATGCGGG	240	0.0	31.653545	8
TCAACGC	2155	0.0	30.403387	4
AATGCGG	250	0.0	30.38576	7
TCGCACC	1145	0.0	29.892181	1
CGCACCA	1165	0.0	29.342474	2
CCGTGCG	180	2.0918378E-10	29.03616	9
GTTCCGG	835	0.0	28.998386	6
GTACATG	4910	0.0	28.948114	1
TCCGGGC	870	0.0	28.924791	8
CTATTAA	455	0.0	28.20869	1
ATTAACC	405	0.0	28.134962	3
TTCCGGG	880	0.0	28.055035	7
TGTTCCG	865	0.0	27.99266	5
CATGGGA	1605	0.0	27.806284	4
ACATGGG	4965	0.0	27.63559	3
>>END_MODULE