##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	924727
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.1761903783495	34.0	33.0	34.0	31.0	34.0
2	33.30617252443154	34.0	33.0	34.0	31.0	34.0
3	33.427286107142976	34.0	34.0	34.0	31.0	34.0
4	36.63409308909549	37.0	37.0	37.0	35.0	37.0
5	36.673531755858754	37.0	37.0	37.0	35.0	37.0
6	36.71826928379944	37.0	37.0	37.0	36.0	37.0
7	36.701506498674746	37.0	37.0	37.0	36.0	37.0
8	36.69209507238352	37.0	37.0	37.0	36.0	37.0
9	38.54716689358048	39.0	39.0	39.0	38.0	39.0
10-11	38.573754199888185	39.0	39.0	39.0	37.5	39.0
12-13	38.56054381455284	39.0	39.0	39.0	38.0	39.0
14-15	40.163844572506264	41.0	40.0	41.0	38.0	41.0
16-17	40.177371808112014	41.0	40.0	41.0	38.0	41.0
18-19	40.18167091476728	41.0	40.0	41.0	38.5	41.0
20-21	40.18854213189406	41.0	40.0	41.0	39.0	41.0
22-23	40.12029279992906	41.0	40.0	41.0	38.0	41.0
24-25	40.08868184880511	41.0	40.0	41.0	38.0	41.0
26-27	40.029248632299044	41.0	40.0	41.0	38.0	41.0
28-29	40.007379475239716	41.0	40.0	41.0	38.0	41.0
30-31	39.96472742766243	41.0	40.0	41.0	38.0	41.0
32-33	39.9311710375062	41.0	40.0	41.0	38.0	41.0
34-35	39.858551767170205	41.0	40.0	41.0	38.0	41.0
36-37	39.754358854018534	41.0	40.0	41.0	37.0	41.0
38-39	39.64611231206615	41.0	40.0	41.0	37.0	41.0
40-41	39.52850625103409	41.0	39.5	41.0	36.5	41.0
42-43	39.45058487531996	41.0	39.0	41.0	36.0	41.0
44-45	39.22259704756107	41.0	39.0	41.0	35.0	41.0
46-47	39.02216978632613	41.0	39.0	41.0	35.0	41.0
48-49	38.92716174611534	40.5	38.0	41.0	35.0	41.0
50-51	38.76966391161932	40.0	38.0	41.0	35.0	41.0
52-53	38.53342878492788	40.0	37.0	41.0	35.0	41.0
54-55	38.342774137664414	40.0	37.0	41.0	35.0	41.0
56-57	38.12609775641892	40.0	36.0	41.0	35.0	41.0
58-59	37.87887560328616	40.0	35.0	41.0	34.5	41.0
60-61	37.64647620324701	39.0	35.0	41.0	34.0	41.0
62-63	37.46863182322999	39.0	35.0	41.0	34.0	41.0
64-65	37.23823950203682	39.0	35.0	41.0	34.0	41.0
66-67	37.00559732764373	38.0	35.0	41.0	34.0	41.0
68-69	36.77705203806096	37.0	35.0	40.5	33.0	41.0
70-71	36.498432510351705	37.0	35.0	40.0	33.0	41.0
72-73	36.22677341528905	36.0	35.0	39.5	33.0	41.0
74-75	35.92590569973625	36.0	35.0	39.0	33.0	41.0
76-77	34.05202183995925	34.5	33.0	37.0	29.5	39.0
78-79	35.31484535435864	35.0	35.0	37.5	33.0	39.0
80-81	35.19248924277111	35.0	35.0	37.0	33.0	39.0
82-83	34.950561084514675	35.0	35.0	37.0	33.0	39.0
84-85	34.69960539705232	35.0	35.0	36.0	33.0	37.5
86-87	34.47710243131216	35.0	35.0	36.0	33.0	37.0
88-89	34.306381234677914	35.0	35.0	36.0	33.0	37.0
90-91	34.144138756627626	35.0	35.0	35.0	33.0	36.0
92-93	34.03428417251794	35.0	35.0	35.0	33.0	36.0
94-95	33.91782709924118	35.0	35.0	35.0	32.0	36.0
96-97	33.866844484912846	35.0	35.0	35.0	32.0	36.0
98-99	33.832443521168955	35.0	34.5	35.0	32.0	35.5
100-101	33.04139005349687	34.5	33.0	35.0	29.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	7.0
14	11.0
15	28.0
16	80.0
17	132.0
18	279.0
19	440.0
20	655.0
21	859.0
22	1209.0
23	1314.0
24	1510.0
25	2202.0
26	3041.0
27	4363.0
28	6082.0
29	6472.0
30	6121.0
31	6667.0
32	7947.0
33	10435.0
34	18593.0
35	39331.0
36	117006.0
37	229655.0
38	316435.0
39	143652.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.396191684903435	20.969964207401485	13.55946200269042	23.074382105004663
2	15.117002098997867	20.993547284766205	40.075070804680735	23.814379811555195
3	18.807064138929654	29.555749967287642	28.765678951733864	22.871506942048843
4	12.282111369085147	17.997203498978617	41.10694291396271	28.61374221797352
5	12.478709932769346	39.53285672420076	35.53805609655606	12.450377246473824
6	27.129087828083314	38.82378258664449	20.83187794884328	13.215251636428912
7	25.85552276509716	31.254305324706642	24.62694395210695	18.26322795808925
8	25.206466340876823	35.283386339968445	21.43875976369242	18.071387555462316
9	26.651000781852375	15.935622080895225	21.343596542547154	36.06978059470525
10-11	25.21290067230653	26.125710615132895	28.54723610319586	20.11415260936471
12-13	25.832164519906957	24.092786303417117	28.551345424108952	21.523703752566973
14-15	23.036636758740688	26.102622720002767	25.664655622686478	25.196084898570064
16-17	22.424672362762198	28.75178295864617	27.05890495248868	21.764639726102946
18-19	22.172814246799323	27.960035772719948	28.012916244469988	21.854233736010737
20-21	23.467965368901655	26.596540783244187	27.407220363397272	22.528273484456886
22-23	23.72931394145452	27.336309378977568	26.601165545783196	22.333211133784715
24-25	23.4495627339214	27.04815387959421	26.745630853779996	22.75665253270439
26-27	22.803176582356848	28.290713411689893	27.204366367850486	21.701743638102766
28-29	22.404481501908748	27.910759281488932	27.783960029847844	21.90079918675448
30-31	22.756672289990483	27.019476165758284	27.6422592784843	22.581592265766933
32-33	22.928209506496795	27.18006586819548	27.890979539333564	22.000745085974163
34-35	23.44763259137497	26.933980755764754	27.079434514717054	22.53895213814322
36-37	22.340655621053497	26.865961365226543	27.84707870891554	22.94630430480442
38-39	22.572283495561393	27.41436121147106	27.052578761083	22.960776531884544
40-41	22.742171473310503	26.952549238856445	27.987665548859287	22.317613738973773
42-43	22.847337646678426	27.028896095820716	27.982042267609792	22.14172398989107
44-45	23.0303980706161	26.611492487677737	27.577374080392797	22.780735361313365
46-47	23.04254517827806	26.559730131622523	27.10842786735151	23.289296822747904
48-49	22.30810407474695	26.53397785275543	27.92342546933126	23.234492603166366
50-51	22.58093469748369	26.966120811872045	27.40679140978905	23.046153080855213
52-53	22.722664485120735	26.91644502455591	27.300991157926152	23.0598993323972
54-55	22.84539112624591	26.705449283950834	27.230631310646274	23.218528279156985
56-57	23.281593454873832	26.572649873061106	26.944841738697846	23.20091493336722
58-59	23.04480137261202	26.698482965136215	27.48599314775809	22.77072251449367
60-61	22.71902212126129	27.55757477419006	27.170263399859373	22.553139704689272
62-63	22.384930903931647	26.7930967734261	27.927215275427237	22.894757047215016
64-65	22.839767844996413	26.72280575780744	27.025489685063807	23.411936712132338
66-67	22.415372320695727	27.25198896539195	26.707341734371333	23.625296979540988
68-69	22.653929213703073	26.9866674164375	27.636534890838053	22.722868479021376
70-71	23.313150800182108	26.795205503894664	27.11627323523591	22.775370460687316
72-73	22.41364208031127	27.16872114688984	27.36596855071821	23.05166822208068
74-75	22.86637029090748	26.44304751564516	27.360020849396633	23.330561344050732
76-77	22.664767725791208	26.08253688119508	27.467487666616925	23.78520772639679
78-79	22.92190509561924	25.908039413533885	27.05338303825094	24.116672452595935
80-81	22.11596850131425	27.188677949568145	26.814654037343992	23.880699511773614
82-83	22.08890888288588	27.19498400600397	27.3320522275884	23.384054883521753
84-85	22.515730547894023	26.235206904625052	27.700687834664468	23.548374712816454
86-87	22.025257184012144	27.477460915491815	27.460699211767363	23.036582688728675
88-89	22.67415139819644	26.31165738645027	26.623046585640953	24.391144629712336
90-91	22.585368438468866	26.49176459647009	26.650622291768272	24.272244673292768
92-93	22.714163207087065	26.561190491896525	27.298975805832427	23.425670495183983
94-95	22.553088641296295	25.967123269894792	27.008079141195186	24.47170894761373
96-97	22.93936480712686	26.60693372206067	27.166396136373223	23.287305334439246
98-99	21.21512619400104	26.69263469110343	27.81853455127838	24.273704563617155
100-101	22.123535159948794	26.48523878421387	27.162238644615112	24.228987411222224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.5
4	2.0
5	0.5
6	1.5
7	6.5
8	11.0
9	13.5
10	14.0
11	15.5
12	13.5
13	14.5
14	19.5
15	18.0
16	20.5
17	27.0
18	28.5
19	30.5
20	43.5
21	54.0
22	56.0
23	74.0
24	106.0
25	144.5
26	215.0
27	293.0
28	388.0
29	627.5
30	1312.0
31	2561.5
32	5162.5
33	9608.0
34	15616.0
35	23987.0
36	36604.5
37	47821.0
38	52418.0
39	54122.5
40	51250.5
41	45110.5
42	39073.0
43	33417.0
44	29772.5
45	27628.5
46	26089.0
47	24995.5
48	24721.0
49	27463.5
50	31918.5
51	31641.5
52	29340.5
53	30113.0
54	33355.5
55	33692.0
56	27535.0
57	22733.0
58	22146.0
59	21981.0
60	18425.0
61	13254.0
62	10425.5
63	6906.0
64	3391.0
65	1811.5
66	1466.5
67	1437.5
68	1032.0
69	518.0
70	210.0
71	104.5
72	86.0
73	79.5
74	56.0
75	36.0
76	23.5
77	10.5
78	7.0
79	3.5
80	2.0
81	1.5
82	2.5
83	3.0
84	1.0
85	0.0
86	0.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47851960632705653
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.732602162584201E-4
22-23	0.0010814002402871334
24-25	4.3256009611485335E-4
26-27	0.0024331505406460503
28-29	0.004001180889062394
30-31	7.569801682009934E-4
32-33	1.6221003604307E-4
34-35	0.0037308308289906106
36-37	0.0017302403844594134
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008597131910282711
46-47	0.07796895732470231
48-49	7.569801682009934E-4
50-51	0.0
52-53	5.407001201435667E-5
54-55	0.0
56-57	0.007515731669995577
58-59	0.03871412860227937
60-61	0.029954786655953594
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	1.0814002402871334E-4
78-79	0.007569801682009934
80-81	0.0057854912855361635
82-83	5.407001201435667E-4
84-85	0.0014598903243876302
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.07029101561866367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	924727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.27287337011156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.36960015895656	21.419377015549408
2	15.881561935741237	10.250872745435217
3	6.408258126927301	6.204387091599393
4	3.2681777909834584	4.218939519976804
5	1.9238708475451223	3.1044420121636467
6	1.203751025618807	2.3309102651362603
7	0.8366232116604713	1.89001644778901
8	0.6289854668068067	1.6239334657517268
9	0.4432095631051092	1.2873281495862294
>10	2.467364691475453	14.744923603838004
>50	0.2689811850333053	6.119471643053424
>100	0.26931774934504515	18.13644737472284
>500	0.022555361507638972	5.099395526201269
>1k	0.007742885293667109	3.569555139196835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2348	0.2539127764194189	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	1813	0.1960578635640573	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1800	0.194652043251684	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1783	0.19281366284319587	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1777	0.1921648226990236	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1736	0.18773108171384637	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	1666	0.18016128003183643	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1505	0.16275073616321356	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	1431	0.1547483743850888	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	1392	0.15053091344796898	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	1382	0.14944951320768185	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	1321	0.14285297174193032	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	1296	0.14014947114121248	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	1272	0.13755411056452338	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1266	0.13690527042035108	No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT	1233	0.13333664962740355	No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC	1204	0.13020058893057088	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	1173	0.12684824818568075	No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA	1168	0.12630754806553718	No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA	1128	0.12198194710438864	No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG	1067	0.11538540563863714	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	1050	0.113547025230149	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	1044	0.11289818508597672	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	999	0.10803188400468464	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	988	0.10684234374036877	No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT	982	0.10619350359619649	No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT	981	0.10608536357216779	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.0814002402871334E-4	0.0
14-15	0.0	0.0	0.0	1.0814002402871334E-4	0.0
16-17	0.0	0.0	0.0	3.2442007208614E-4	0.0
18-19	0.0	0.0	0.0	3.2442007208614E-4	0.0
20-21	0.0	0.0	0.0	3.784900841004967E-4	0.0
22-23	0.0	0.0	0.0	5.407001201435667E-4	0.0
24-25	0.0	0.0	0.0	0.0014058203123732734	0.0
26-27	0.0	0.0	0.0	0.00227094050460298	0.0
28-29	0.0	0.0	0.0	0.004325600961148533	0.0
30-31	0.0	0.0	0.0	0.010543652342799551	0.0
32-33	0.0	0.0	0.0	0.028494896331565968	0.0
34-35	0.0	0.0	0.0	0.0526641917019834	0.0
36-37	0.0	0.0	0.0	0.09251379055656427	0.0
38-39	0.0	0.0	0.0	0.16518388670385964	0.0
40-41	0.0	0.0	0.0	0.25250695610704565	0.0
42-43	0.0	0.0	0.0	0.33323348404448017	0.0
44-45	0.0	0.0	0.0	0.42569320458903004	0.0
46-47	0.0	0.0	0.0	0.5418355903958683	0.0
48-49	0.0	0.0	0.0	0.6597082165871657	0.0
50-51	0.0	0.0	0.0	0.7900169455417653	0.0
52-53	0.0	0.0	0.0	0.9236239452292406	0.0
54-55	0.0	0.0	0.0	1.056311754712472	0.0
56-57	0.0	0.0	0.0	1.2124118793979197	0.0
58-59	0.0	0.0	0.0	1.3853818478318467	0.0
60-61	0.0	0.0	0.0	1.5730047895216641	0.0
62-63	0.0	0.0	0.0	1.77674059479176	0.0
64-65	0.0	0.0	0.0	1.9773403393650233	0.0
66-67	0.0	0.0	0.0	2.2034070595970485	0.0
68-69	0.0	0.0	0.0	2.472837929464588	0.0
70-71	0.0	0.0	0.0	2.7668706547986597	0.0
72-73	0.0	0.0	0.0	3.0683650417907122	0.0
74-75	0.0	0.0	0.0	3.376726320308588	0.0
76-77	0.0	0.0	0.0	3.6948202009890485	0.0
78-79	0.0	0.0	0.0	4.061306742422358	0.0
80-81	0.0	0.0	0.0	4.456180040163205	0.0
82-83	0.0	0.0	0.0	4.87387088297411	0.0
84-85	0.0	0.0	0.0	5.299401877527098	0.0
86-87	0.0	0.0	0.0	5.7475882071141	0.0
88-89	0.0	0.0	0.0	6.245356737718268	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATTCG	20	0.0020764738	71.22814	4
GGTATCA	705	0.0	53.499905	1
GTATCAA	1580	0.0	47.139236	1
CCGTGCG	135	0.0	42.20927	9
ATCAACG	1975	0.0	36.305317	3
CTATTAA	380	0.0	35.179485	1
TCAACGC	2185	0.0	33.03334	4
AACCGTG	205	0.0	32.42907	7
ACCCTTG	385	0.0	30.834694	7
CATGGGA	1630	0.0	30.00613	4
GTACATG	5060	0.0	29.533087	1
AAACCGT	210	1.8189894E-12	29.39574	6
ACATGGG	4985	0.0	28.767498	3
CCCTTGG	380	0.0	28.74118	8
AACCCTT	400	0.0	28.491255	6
GGACCGT	100	4.962445E-5	28.491255	6
CGCAGAG	2560	0.0	27.823492	8
TCCGGGC	740	0.0	27.592886	8
TCGCACC	920	0.0	27.504456	1
CGCACCA	970	0.0	26.924726	2
>>END_MODULE