FastQCFastQC Report
Fri 10 Feb 2017
SRR3224476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953035
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG22200.23294002843547196No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA16050.168409344882402No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA14150.14847303614242918No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA11720.12297554654341131No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG9850.10335402162564859No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA9610.10083575104796781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13550.043.14691
GGTATCA6650.040.7414971
ATCAACG16900.032.0308723
CGTTATA753.0660155E-431.6878321
TCAACGC17350.031.20014
AATGCGG1901.2732926E-1129.9901247
CCGTGCG804.487925E-429.6933089
TCGCACC6750.028.8711341
GTACATG38450.028.3088971
CGCAGAG19650.027.3065328
TACATGG39300.026.581582
ACATGGG38500.026.1472343
CGCACCA7450.026.1323362
ACGCAGA21150.025.3698967
TATCAAC22250.025.182722
CTGTGCG8900.025.0891769
TTACACG4950.024.9412824
CAACGCA22550.024.0053985
ATGCGGG2202.1755113E-923.7421848
CATGGGG24800.023.5507144
Produced by FastQC (version 0.11.5)