##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224476_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	953035
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11046288961056	34.0	31.0	34.0	31.0	34.0
2	32.26729343623267	34.0	31.0	34.0	31.0	34.0
3	32.40602811019532	34.0	31.0	34.0	31.0	34.0
4	35.643893456168975	37.0	37.0	37.0	35.0	37.0
5	35.6511324348004	37.0	37.0	37.0	35.0	37.0
6	35.75597223606688	37.0	37.0	37.0	35.0	37.0
7	35.716612716217135	37.0	37.0	37.0	35.0	37.0
8	35.7516303178792	37.0	37.0	37.0	35.0	37.0
9	37.49240688956859	39.0	39.0	39.0	35.0	39.0
10-11	37.568641760271134	39.0	39.0	39.0	36.0	39.0
12-13	37.57164112545709	39.0	39.0	39.0	35.0	39.0
14-15	39.09249817687703	41.0	40.0	41.0	37.0	41.0
16-17	38.94022517536082	41.0	40.0	41.0	36.5	41.0
18-19	39.020775207626166	41.0	40.0	41.0	36.5	41.0
20-21	39.082038959744395	41.0	40.0	41.0	37.0	41.0
22-23	39.04626797546784	41.0	40.0	41.0	36.5	41.0
24-25	39.00186299558778	41.0	40.0	41.0	36.0	41.0
26-27	38.856719323004924	41.0	40.0	41.0	35.5	41.0
28-29	38.79185549324002	41.0	39.5	41.0	35.0	41.0
30-31	38.73631660956838	41.0	39.0	41.0	35.0	41.0
32-33	38.56357479001296	41.0	39.0	41.0	35.0	41.0
34-35	38.583138079923614	41.0	39.0	41.0	35.0	41.0
36-37	38.584045181971284	41.0	39.0	41.0	35.0	41.0
38-39	38.48165649740041	41.0	39.0	41.0	35.0	41.0
40-41	38.41153472852518	41.0	39.0	41.0	35.0	41.0
42-43	38.30224335937295	40.5	38.5	41.0	34.5	41.0
44-45	38.153557319510824	40.0	38.0	41.0	34.0	41.0
46-47	38.01906960394949	40.0	38.0	41.0	34.0	41.0
48-49	37.85940233044956	40.0	38.0	41.0	33.0	41.0
50-51	36.62345611651199	39.0	36.0	40.0	31.5	40.5
52-53	36.94685662121538	39.5	36.5	40.5	32.5	40.5
54-55	37.417345113243485	40.0	36.5	41.0	33.0	41.0
56-57	37.303095898891435	40.0	36.0	41.0	33.0	41.0
58-59	37.18081759851422	40.0	35.5	41.0	33.0	41.0
60-61	36.96931907012859	39.5	35.0	41.0	32.5	41.0
62-63	36.708597270824264	39.0	35.0	41.0	32.0	41.0
64-65	36.48973437491803	39.0	35.0	41.0	32.0	41.0
66-67	36.26217557592324	38.0	35.0	41.0	31.0	41.0
68-69	35.98228711432424	37.0	35.0	40.0	31.0	41.0
70-71	35.69010477054883	37.0	35.0	40.0	31.0	41.0
72-73	35.361153577780456	36.0	35.0	39.0	30.5	41.0
74-75	35.047586919682914	36.0	35.0	39.0	30.0	41.0
76-77	34.68274564942526	35.5	35.0	38.5	30.0	40.0
78-79	34.3910695829639	35.0	35.0	37.0	30.0	39.0
80-81	34.03887475276354	35.0	34.0	37.0	29.5	39.0
82-83	33.73670169511088	35.0	34.0	36.5	29.0	38.0
84-85	33.47310277167155	35.0	34.0	36.0	29.0	37.0
86-87	33.22569685268642	35.0	34.0	36.0	29.0	37.0
88-89	33.04952756194683	35.0	34.0	35.0	29.0	36.5
90-91	32.862886462721725	35.0	34.0	35.0	28.5	36.0
92-93	32.71325712067238	35.0	34.0	35.0	27.0	36.0
94-95	32.57039668007995	35.0	34.0	35.0	27.0	36.0
96-97	32.439879437796094	35.0	34.0	35.0	27.0	35.0
98-99	32.380339651744166	35.0	33.0	35.0	26.0	35.0
100-101	31.225898314332632	34.5	31.0	35.0	22.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	11.0
7	1022.0
8	4928.0
9	3369.0
10	1967.0
11	1938.0
12	2062.0
13	2009.0
14	1858.0
15	1775.0
16	1745.0
17	1849.0
18	1901.0
19	2038.0
20	2353.0
21	2978.0
22	3439.0
23	3435.0
24	3621.0
25	3753.0
26	4116.0
27	4486.0
28	4898.0
29	5625.0
30	7216.0
31	8998.0
32	12141.0
33	17183.0
34	28717.0
35	52329.0
36	115488.0
37	213689.0
38	323849.0
39	106240.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09886474922348	19.31155719755717	12.797945536543642	24.791632516675705
2	15.924283997964398	20.270923942982158	38.68430855110253	25.120483507950915
3	19.406568293018182	26.614818164743507	28.50132157018697	25.477291972051336
4	12.86364089461562	16.66927237719496	38.67990157759159	31.787185150597825
5	13.476420068517944	38.85817414890324	34.63713294894731	13.028272833631505
6	29.95577287297948	37.52013304862886	19.071177868598742	13.452916209792926
7	27.199945437470817	31.28552466593567	23.056655841600783	18.457874054992736
8	25.67502767474437	35.397335879584695	20.883912972765952	18.043723472904983
9	26.680027491120473	15.656612821145078	20.18782101391869	37.475538673815755
10-11	25.412655358932252	25.546333555430806	28.36349137230007	20.677519713336867
12-13	25.928848363386443	23.172915999937043	28.42566117718657	22.472574459489945
14-15	23.085292197363643	25.091531451340327	25.28776774524026	26.53540860605577
16-17	22.90169678755976	28.156760951309895	26.357015275183258	22.584526985947086
18-19	22.972014210672697	27.572430875152836	26.987631257182738	22.467923656991726
20-21	23.716991213052253	26.2417035195027	26.84270303349663	23.198602233948414
22-23	23.840180342310198	26.792004712251327	26.251691759125002	23.116123186313473
24-25	23.235998497159084	26.83547040389899	26.59477447941045	23.333756619531474
26-27	23.123967115583373	27.607433095321788	26.787998342138536	22.480601446956303
28-29	22.95146558101224	27.349835000813194	27.051892113091334	22.646807305083232
30-31	23.052721043823155	27.059237068942902	26.812446552330186	23.075595334903753
32-33	22.98997413526261	27.085521518097444	26.979491833983012	22.94501251265693
34-35	23.36734747412215	26.768639137072615	26.730445366644457	23.13356802216078
36-37	22.92291021103202	26.701388251160374	27.360486137166877	23.01521540064073
38-39	22.98830733973353	26.9334128024294	26.714837810640397	23.363442047196667
40-41	23.275068273218796	26.554333939331933	27.306611680819753	22.86398610662952
42-43	23.295113515691565	26.794865875617525	27.11307287753569	22.79694773115522
44-45	23.125107236798264	26.530659361359284	26.939352901021024	23.404880500821427
46-47	23.176063838159145	26.26713604432156	27.16322065821297	23.39357945930632
48-49	22.78746320964078	26.715230815237632	27.175602155219902	23.321703819901682
50-51	22.913219346613715	26.943449086339953	26.906934162963587	23.236397404082744
52-53	22.996112419795704	26.734694948244293	26.683647505075893	23.58554512688411
54-55	23.067253563615186	26.427885649530186	26.974979932531333	23.52988085432329
56-57	22.935254214168417	26.69692088957908	26.944183581925113	23.423641314327387
58-59	23.191240062400073	26.691510557166747	27.050631058256336	23.066618322176847
60-61	23.010246809153703	27.021446516987414	26.87457628541624	23.09373038844264
62-63	22.75222877390671	26.572054418398643	27.25580047986599	23.41991632782866
64-65	23.41802083918367	26.089291141273623	26.73170403971108	23.760983979831625
66-67	22.75874445297418	26.64419433598618	26.48483458751957	24.112226623520073
68-69	22.746908560546046	26.69387797929772	27.460166730497832	23.099046729658408
70-71	23.202033503491474	26.389377095279816	27.084944414423397	23.323644986805313
72-73	22.92659765905764	26.81748309348555	27.17418562801996	23.08173361943685
74-75	22.977067998551995	26.670426584543065	27.068575655668468	23.28392976123647
76-77	22.970982177989267	26.3265777227489	26.80022244723436	23.90221765202747
78-79	23.176883827242637	25.830277665252698	27.21388137361288	23.778957133891783
80-81	22.978459367608103	26.75471060254347	26.640534856936476	23.62629517291195
82-83	22.78465398505081	27.03320525741161	26.93011463844797	23.25202611908961
84-85	23.00233375719164	26.22808265079551	27.31524509698448	23.454338495028367
86-87	22.824926376793336	26.98268214197599	27.066515483183462	23.125875998047213
88-89	23.09972426229405	26.389840844657964	26.647157550458324	23.86327734258967
90-91	22.91998719879123	26.24615045617422	26.87529838883147	23.958563956203076
92-93	22.979412831002	26.31258351945679	27.217466171176913	23.490537478364296
94-95	23.16383973306332	26.114780674371872	27.203932699218814	23.517446893345994
96-97	23.559680389492517	26.40102409670159	26.96102451641335	23.07827099739254
98-99	22.65055019773961	26.428064019575643	27.05806063035337	23.863325152331374
100-101	23.224500336450795	26.087848824941663	26.693197815221854	23.99445302338569
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	3.0
4	2.5
5	2.0
6	2.5
7	3.5
8	7.5
9	12.0
10	11.5
11	10.0
12	11.5
13	12.5
14	15.5
15	22.5
16	23.5
17	25.0
18	35.0
19	45.5
20	47.5
21	50.0
22	61.0
23	76.5
24	86.0
25	114.5
26	158.0
27	191.5
28	235.5
29	314.5
30	541.0
31	1093.5
32	2383.0
33	5102.5
34	9392.5
35	16200.5
36	26122.5
37	35950.5
38	44247.0
39	50112.5
40	52416.5
41	51909.0
42	49463.0
43	45951.5
44	42413.0
45	39636.0
46	36810.0
47	32703.5
48	29559.5
49	29032.0
50	29573.5
51	28765.0
52	27446.0
53	28364.5
54	30611.0
55	31358.5
56	27517.0
57	23895.5
58	23158.0
59	22457.5
60	19435.5
61	15145.0
62	12518.0
63	9239.5
64	5744.0
65	3772.5
66	2896.5
67	2573.5
68	1943.0
69	1224.5
70	792.5
71	540.0
72	409.0
73	317.0
74	222.5
75	146.5
76	103.0
77	61.5
78	36.0
79	23.0
80	15.5
81	15.5
82	8.5
83	6.5
84	9.5
85	9.0
86	7.5
87	7.0
88	7.5
89	6.0
90	4.0
91	4.0
92	3.0
93	3.0
94	2.5
95	2.0
96	2.5
97	3.5
98	3.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14175764793528045
2	0.0
3	2.0985588147339815E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03745927484300157
16-17	0.07407912616010955
18-19	0.024133426369440786
20-21	0.03903319395405205
22-23	0.06547503501970021
24-25	0.018152533747448938
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02282182711023205
38-39	0.004616829392414759
40-41	0.006557996296043692
42-43	0.004092189688731263
44-45	0.012643816858772238
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.015896583021609907
60-61	0.01605397493271496
62-63	0.02822561605817205
64-65	0.04768974906482973
66-67	0.016736006547503502
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01689339845860855
80-81	0.058339935049604684
82-83	0.049840771849932054
84-85	0.029327359435907394
86-87	0.05771036740518449
88-89	0.0333670851542703
90-91	0.0
92-93	0.004039725718362914
94-95	0.0
96-97	0.0
98-99	0.001468991170313787
100-101	0.04658800568709439
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	953035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.14395072886497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.72133693519524	31.474965993530475
2	15.110872941565889	13.643290070883788
3	5.95498041499588	8.064940273377896
4	2.951121972748176	5.3290121973045785
5	1.7050472810190447	3.848628522235447
6	1.0851118810288174	2.9391742375482552
7	0.7158582007814885	2.262166714145341
8	0.4968117230975815	1.7942435159231784
9	0.36764938306531453	1.4937431071138347
>10	1.657677638386696	13.081847178308983
>50	0.11899797391766212	3.756578801155449
>100	0.10655385044895513	9.472295422681706
>500	0.007041003308159597	2.15974395725799
>1k	9.388004410879464E-4	0.6793700085330766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	2220	0.23294002843547196	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1605	0.168409344882402	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1415	0.14847303614242918	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1172	0.12297554654341131	No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG	985	0.10335402162564859	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	961	0.10083575104796781	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0492794073669908E-4	0.0
5	0.0	0.0	0.0	1.0492794073669908E-4	0.0
6	0.0	0.0	0.0	1.0492794073669908E-4	0.0
7	0.0	0.0	0.0	1.0492794073669908E-4	0.0
8	0.0	0.0	0.0	1.0492794073669908E-4	0.0
9	0.0	0.0	0.0	3.147838222100972E-4	0.0
10-11	0.0	0.0	0.0	4.197117629467963E-4	0.0
12-13	0.0	0.0	0.0	6.820316147885439E-4	0.0
14-15	0.0	0.0	0.0	0.0011017433777353403	5.246397036834954E-5
16-17	0.0	0.0	0.0	0.0013115992592087383	1.0492794073669908E-4
18-19	0.0	0.0	0.0	0.0017313110221555347	1.0492794073669908E-4
20-21	0.0	0.0	0.0	0.0019936308739972822	1.0492794073669908E-4
22-23	0.0	0.0	0.0	0.002833054399890875	1.0492794073669908E-4
24-25	0.0	0.0	0.0	0.0037249418961528167	1.0492794073669908E-4
26-27	0.0	0.0	0.0	0.005928428651623498	1.0492794073669908E-4
28-29	0.0	0.0	0.0	0.008813947021882722	1.0492794073669908E-4
30-31	0.0	0.0	0.0	0.01888702933260583	1.0492794073669908E-4
32-33	0.0	0.0	0.0	0.03856101822073691	1.0492794073669908E-4
34-35	0.0	0.0	0.0	0.06416343576049148	1.0492794073669908E-4
36-37	0.0	0.0	0.0	0.11069897747721752	1.0492794073669908E-4
38-39	0.0	0.0	0.0	0.20403238076251135	1.0492794073669908E-4
40-41	0.0	0.0	0.0	0.32244356188387624	1.0492794073669908E-4
42-43	0.0	0.0	0.0	0.4314636923093066	1.0492794073669908E-4
44-45	0.0	0.0	0.0	0.5476713866752008	1.0492794073669908E-4
46-47	0.0	0.0	0.0	0.6780443530405494	1.0492794073669908E-4
48-49	0.0	0.0	0.0	0.8148179237908366	1.0492794073669908E-4
50-51	0.0	0.0	0.0	0.9638680636073177	1.0492794073669908E-4
52-53	0.0	0.0	0.0	1.1076718063869637	1.0492794073669908E-4
54-55	0.0	0.0	0.0	1.2478030712408255	1.0492794073669908E-4
56-57	0.0	0.0	0.0	1.407136149249503	1.0492794073669908E-4
58-59	0.0	0.0	0.0	1.5843069771834193	1.0492794073669908E-4
60-61	0.0	0.0	0.0	1.7839323844349892	1.0492794073669908E-4
62-63	0.0	0.0	0.0	1.9933160901750722	1.0492794073669908E-4
64-65	0.0	0.0	0.0	2.1863310371602305	1.0492794073669908E-4
66-67	0.0	0.0	0.0	2.403741730366671	1.0492794073669908E-4
68-69	0.0	0.0	0.0	2.6584018425346394	1.573919111050486E-4
70-71	0.0	0.0	0.0	2.9271222987613257	2.623198518417477E-4
72-73	0.0	0.0	0.0	3.2179825504834554	3.147838222100972E-4
74-75	0.0	0.0	0.0	3.5135120955683687	3.147838222100972E-4
76-77	0.0	0.0	0.0	3.8042674193497614	3.147838222100972E-4
78-79	0.0	0.0	0.0	4.1335837613518915	3.147838222100972E-4
80-81	0.0	0.0	0.0	4.4934865980787695	3.672477925784468E-4
82-83	0.0	0.0	0.0	4.902495711070422	4.197117629467963E-4
84-85	0.0	0.0	0.0	5.295240993247887	4.197117629467963E-4
86-87	0.0	0.0	0.0	5.719674513527835	4.7217573331514583E-4
88-89	0.0	0.0	0.0	6.171126978547482	5.246397036834954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1355	0.0	43.1469	1
GGTATCA	665	0.0	40.741497	1
ATCAACG	1690	0.0	32.030872	3
CGTTATA	75	3.0660155E-4	31.687832	1
TCAACGC	1735	0.0	31.2001	4
AATGCGG	190	1.2732926E-11	29.990124	7
CCGTGCG	80	4.487925E-4	29.693308	9
TCGCACC	675	0.0	28.871134	1
GTACATG	3845	0.0	28.308897	1
CGCAGAG	1965	0.0	27.306532	8
TACATGG	3930	0.0	26.58158	2
ACATGGG	3850	0.0	26.147234	3
CGCACCA	745	0.0	26.132336	2
ACGCAGA	2115	0.0	25.369896	7
TATCAAC	2225	0.0	25.18272	2
CTGTGCG	890	0.0	25.089176	9
TTACACG	495	0.0	24.941282	4
CAACGCA	2255	0.0	24.005398	5
ATGCGGG	220	2.1755113E-9	23.742184	8
CATGGGG	2480	0.0	23.550714	4
>>END_MODULE