FastQCFastQC Report
Fri 10 Feb 2017
SRR3224476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953035
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT76030.797767133421123No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT34550.3625260352452953RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG16270.17071775957860938RNA PCR Primer, Index 33 (96% over 29bp)
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG16190.1698783360527158No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT13480.14144286411307036RNA PCR Primer, Index 33 (95% over 24bp)
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA13460.14123300823159693No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA12910.1354619714910785No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT12670.13294370091339772No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA11750.12329033036562141No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG10760.1129024642326882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG804.2291504E-947.4921849
GGTATCA7150.042.6874161
GTATCAA13950.040.3397981
ATCAACG15400.035.7733353
TCAACGC15500.035.2361374
CAACGCA19250.028.6186645
CGCAGAG19350.028.2253288
GTACATG37100.028.151151
AACGCAG19850.027.5143646
TCCGGGC6300.026.3845448
TATCAAC21450.026.3476432
TACATGG38900.025.7605422
ACGCAGA21550.025.3438557
CGTCCGA751.062248E-625.32916310-11
ACATGGG38300.025.2960993
GTTCCGG6400.025.2302236
GTATAGT2701.8189894E-1224.728071
CTCGTCC1601.8616647E-623.7460929
TTCCGGG7000.023.067637
CATGGGG25750.023.0544574
Produced by FastQC (version 0.11.5)