FastQCFastQC Report
Fri 10 Feb 2017
SRR3224475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346417
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG4270.12326184915867291No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC4030.116333782695422No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA3630.10478700525667042No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA3600.10392099694876407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT504.251051E-756.9654466
GGTATCA3350.045.3849331
CCGTGCG652.5718018E-643.876579
GTATCAA5500.038.873741
GTCTAAT2000.033.2586481
TCAACGC6650.030.6956654
ATCAACG6700.030.4665933
TACCGTT650.00585583929.2130495
ACCGTGC1004.958852E-528.4868348
CGCAGAG7350.027.776288
CGTTGTC700.00840253527.1303188
ACGCAGA7600.026.858717
CTAATGT2451.0913936E-1125.18883
CAACGCA8150.025.0461565
TCTAATG2351.7644197E-1024.2406162
GTATAAT2751.8189894E-1224.1881071
AACGCAG8750.023.8712336
GTATTGG1201.7044456E-423.7561781
AACCGTG1201.7144917E-423.7356037
TAGGACC1201.7144917E-423.7356034
Produced by FastQC (version 0.11.5)