##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224475_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346417
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.230895135054	34.0	31.0	34.0	31.0	34.0
2	32.38936022192906	34.0	31.0	34.0	31.0	34.0
3	32.5312499097908	34.0	31.0	34.0	31.0	34.0
4	35.835109131480266	37.0	37.0	37.0	35.0	37.0
5	35.84112788922022	37.0	37.0	37.0	35.0	37.0
6	35.94775660547837	37.0	37.0	37.0	35.0	37.0
7	35.90630944786197	37.0	37.0	37.0	35.0	37.0
8	35.93714800370652	37.0	37.0	37.0	35.0	37.0
9	37.675858863739364	39.0	39.0	39.0	35.0	39.0
10-11	37.748056533022336	39.0	39.0	39.0	36.0	39.0
12-13	37.761408937783074	39.0	39.0	39.0	35.0	39.0
14-15	39.27671996466687	41.0	40.0	41.0	37.0	41.0
16-17	39.1112012401239	41.0	40.0	41.0	36.5	41.0
18-19	39.205480677911304	41.0	40.0	41.0	36.5	41.0
20-21	39.27859198595912	41.0	40.0	41.0	37.0	41.0
22-23	39.24105918589446	41.0	40.0	41.0	37.0	41.0
24-25	39.19964378191601	41.0	40.0	41.0	36.0	41.0
26-27	39.05793018241022	41.0	39.5	41.0	36.0	41.0
28-29	38.97328653039545	41.0	39.0	41.0	35.5	41.0
30-31	38.89573692976961	41.0	39.0	41.0	35.0	41.0
32-33	38.70549944142464	41.0	39.0	41.0	35.0	41.0
34-35	38.717026012002876	41.0	39.0	41.0	35.0	41.0
36-37	38.724574717753455	41.0	39.0	41.0	35.0	41.0
38-39	38.628095618863966	41.0	39.0	41.0	35.0	41.0
40-41	38.56115606335717	40.5	39.0	41.0	35.0	41.0
42-43	38.43892765077926	40.0	38.5	41.0	34.5	41.0
44-45	38.30217483553059	40.0	38.0	41.0	34.0	41.0
46-47	38.16152931293787	40.0	38.0	41.0	33.5	41.0
48-49	37.999966803014864	40.0	38.0	41.0	33.0	41.0
50-51	36.788416850212315	39.0	36.5	40.0	31.5	40.5
52-53	37.147082851014815	39.5	37.0	40.5	32.5	40.5
54-55	37.66035154163912	40.0	37.0	41.0	33.0	41.0
56-57	37.57021018021633	40.0	37.0	41.0	33.0	41.0
58-59	37.47714315405999	40.0	36.0	41.0	33.0	41.0
60-61	37.272641065536625	40.0	35.5	41.0	32.5	41.0
62-63	37.020455116232746	39.5	35.0	41.0	32.0	41.0
64-65	36.81079017484708	39.0	35.0	41.0	32.0	41.0
66-67	36.569868106934706	39.0	35.0	41.0	31.0	41.0
68-69	36.28675844430268	38.0	35.0	41.0	31.0	41.0
70-71	35.9931036871747	37.0	35.0	40.0	31.0	41.0
72-73	35.66843717254061	37.0	35.0	40.0	30.5	41.0
74-75	35.36838983075311	36.0	35.0	39.0	30.0	41.0
76-77	34.9940707297852	36.0	35.0	39.0	30.0	40.5
78-79	34.69690863901021	35.5	35.0	38.5	30.0	39.5
80-81	34.32343389614251	35.0	34.0	37.0	29.0	39.0
82-83	34.0024204932206	35.0	34.0	37.0	29.0	39.0
84-85	33.71569524590306	35.0	34.0	36.5	29.0	38.0
86-87	33.43467988002898	35.0	34.0	36.0	29.0	37.0
88-89	33.23116359762945	35.0	34.0	36.0	29.0	37.0
90-91	33.01929033505861	35.0	34.0	35.0	28.5	36.0
92-93	32.85414399408805	35.0	34.0	35.0	27.5	36.0
94-95	32.70509819090864	35.0	34.0	35.0	27.0	36.0
96-97	32.562227026964614	35.0	33.5	35.0	27.0	36.0
98-99	32.495961514590796	35.0	33.0	35.0	27.0	35.0
100-101	31.39696521821966	34.5	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	55.0
8	220.0
9	172.0
10	195.0
11	347.0
12	518.0
13	609.0
14	681.0
15	729.0
16	697.0
17	835.0
18	875.0
19	1049.0
20	1131.0
21	1490.0
22	1793.0
23	1672.0
24	1589.0
25	1673.0
26	1737.0
27	1887.0
28	2132.0
29	2491.0
30	2963.0
31	3598.0
32	4801.0
33	6836.0
34	10423.0
35	18016.0
36	37366.0
37	72541.0
38	117887.0
39	47399.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30268863833478	20.718704827984965	13.382769586585718	23.595836947094536
2	14.876002043779607	20.927090760557363	40.70065845498345	23.496248740679583
3	17.899577386725788	27.83647406586301	29.985624220590275	24.278324326820933
4	11.863159140573355	17.639434554308824	40.69113236359648	29.806273941521344
5	12.450601442769841	39.09738840761279	35.86457939419832	12.587430755419046
6	28.326265743309364	39.36902634685942	19.467866761735134	12.836841148096081
7	25.167356105502904	32.394772773853475	24.953740722886	17.484130397757617
8	24.18357066772127	36.767826059344664	21.682538674487684	17.366064598446382
9	25.281957871582517	16.43481699801107	21.787614349180323	36.4956107812261
10-11	24.06232950461438	26.693984417623845	29.660495876357107	19.583190201404665
12-13	24.414506216496303	24.59636796115664	29.788376436491284	21.200749385855776
14-15	21.963510141847248	26.404178025227555	26.662922191932726	24.969389640992468
16-17	21.626677182232736	29.14126247459581	27.650016538905426	21.58204380426603
18-19	21.481133914198473	28.486309725761394	28.698972778263037	21.333583581777102
20-21	22.532156615859115	27.474918705995833	28.517590116497338	21.475334561647713
22-23	22.276480537056216	27.847081100052428	28.01924412919738	21.85719423369398
24-25	21.755844755779794	27.856622131756925	28.181575395364618	22.205957717098663
26-27	21.837265492166953	28.16201283424312	28.24471662764818	21.75600504594174
28-29	21.752829682146086	28.242695941596402	28.347049942699115	21.6574244335584
30-31	21.88994766423126	27.771731756813324	28.45616698949532	21.882153589460103
32-33	21.886916635153586	27.7118328488498	28.553448589416803	21.847801926579816
34-35	22.018549897955356	27.668965437608435	28.327564755771224	21.984919908664992
36-37	21.87219384587844	27.541094367624595	28.536367757415466	22.0503440290815
38-39	21.954162685227153	27.923290771624632	28.028371906385413	22.09417463676281
40-41	22.169873279954732	27.46919327296846	28.51381449132273	21.847118955754077
42-43	22.091514400072747	27.60154674665023	28.48764955420614	21.819289299070885
44-45	22.055174384950394	27.820610350935187	27.967705474854526	22.15650978925989
46-47	22.286579469252374	27.62321133200738	27.69090431474206	22.399304883998187
48-49	21.857616687402754	27.74517416870419	27.977264395223102	22.419944748669955
50-51	21.875081188278866	27.951572815421876	27.851693190576675	22.321652805722582
52-53	22.192906237280503	28.078298697812176	27.52087801695644	22.207917047950883
54-55	21.993579991744053	27.498217466232894	27.91823149556748	22.589971046455574
56-57	22.159420582708123	27.808970114053295	27.656841321297744	22.374767981940842
58-59	22.186437154620364	27.640909117156237	28.037175408103753	22.135478320119645
60-61	22.349403503909272	27.969343681068032	27.761753802446037	21.919499012576654
62-63	21.835793011645332	27.713610861662225	28.14240627860441	22.308189848088034
64-65	22.55228572707594	27.341359407243104	27.67089342419346	22.435461441487494
66-67	21.984830680482855	27.648784346786698	27.611395178962745	22.754989793767702
68-69	22.0856655418181	27.73319438711149	28.059968188628154	22.12117188244226
70-71	22.279651402789124	27.40165753990133	27.831919334212813	22.48677172309673
72-73	22.169668347685015	27.633892101138223	28.02431751328601	22.17212203789075
74-75	22.085232537664147	27.55537401455471	27.897735965613695	22.461657482167446
76-77	22.01450852585179	27.563168089325867	27.716595894543282	22.705727490279056
78-79	22.221676867998614	27.000952765908302	27.857431574084767	22.919938792008317
80-81	22.025957746092402	27.79229181740327	27.594005485007212	22.587744951497115
82-83	21.937651902545536	27.91858673409708	27.592072979651018	22.551688383706374
84-85	22.056715163748034	27.480043140601108	27.97598679793653	22.48725489771433
86-87	21.716826709916134	27.933167441007367	28.06083617242211	22.289169676654385
88-89	22.238521724943002	27.299806090463203	27.668855067420743	22.79281711717305
90-91	22.114532485414976	27.525785397367912	27.698987058949186	22.660695058267926
92-93	22.08383250819128	27.517645530520635	27.97043922576175	22.428082735526335
94-95	22.206762370207006	27.21084704272596	27.90018965581943	22.6822009312476
96-97	22.329591215211725	27.507021884029943	27.840435082573894	22.322951818184443
98-99	21.711367644978683	27.635654551070544	27.86341736597645	22.789560437974327
100-101	22.08863583526228	27.29571318620861	27.767902396901746	22.847748581627364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.5
4	2.5
5	3.5
6	5.0
7	6.0
8	14.5
9	19.5
10	21.0
11	22.5
12	24.5
13	29.0
14	40.0
15	45.0
16	40.5
17	52.0
18	67.0
19	71.0
20	75.5
21	89.0
22	106.0
23	132.0
24	181.5
25	244.5
26	348.5
27	491.0
28	728.0
29	1260.5
30	2209.0
31	3717.0
32	5831.5
33	8355.0
34	11214.0
35	13720.0
36	16342.0
37	18541.0
38	18765.5
39	17644.5
40	16026.0
41	14407.0
42	12781.5
43	11355.0
44	10349.5
45	9927.0
46	9944.5
47	9611.0
48	9556.5
49	10047.5
50	10524.0
51	10382.0
52	10019.5
53	10187.0
54	10426.5
55	10278.0
56	8832.5
57	7354.5
58	6633.0
59	6113.5
60	5266.5
61	4066.0
62	3300.5
63	2467.0
64	1589.5
65	1097.0
66	860.0
67	725.0
68	584.0
69	390.0
70	234.5
71	163.0
72	110.5
73	86.0
74	77.0
75	54.0
76	39.0
77	23.5
78	11.0
79	7.5
80	7.0
81	8.0
82	5.5
83	3.0
84	2.0
85	0.5
86	0.5
87	0.0
88	1.0
89	2.0
90	1.5
91	3.0
92	3.0
93	1.5
94	2.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14924209839586394
2	0.0
3	2.8866943596878903E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03723835723997379
16-17	0.07606439637777591
18-19	0.027279261699050567
20-21	0.04127972934353684
22-23	0.06740431329871224
24-25	0.01717583144014295
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.023093554877503124
38-39	0.005196049847438202
40-41	0.006783731745266542
42-43	0.003608367949609863
44-45	0.012268451028673535
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.017031496722158552
60-61	0.017608835594096133
62-63	0.02828960472494133
64-65	0.04921813883267854
66-67	0.017320166158127343
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016454157850220977
80-81	0.0578782219117422
82-83	0.0529708415002728
84-85	0.031031964366644824
86-87	0.06018757739949252
88-89	0.03507333647020787
90-91	0.0
92-93	0.003464033231625469
94-95	0.0
96-97	0.0
98-99	0.0011546777438751561
100-101	0.04546543616508428
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	346417.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.65088985499526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.77811791452693	49.9817007830185
2	11.952067596756423	14.976153410876892
3	3.5979672910879716	6.762475573674845
4	1.6089533168201862	4.032094281357232
5	0.8585044230822116	2.6893033025274944
6	0.5397827421275017	2.029072147359445
7	0.32457245463441425	1.4234327173686292
8	0.22841242245086493	1.1448193216385427
9	0.16612583216221677	0.9367138091578026
>10	0.8434959897042558	9.915136803905561
>50	0.07358905310608677	3.1377115576781667
>100	0.028410963540938847	2.9713862914368634
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	427	0.12326184915867291	No Hit
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	403	0.116333782695422	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	363	0.10478700525667042	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	360	0.10392099694876407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.4433471798439452E-4	0.0
22-23	0.0	0.0	0.0	4.3300415395318355E-4	0.0
24-25	0.0	0.0	0.0	0.0010103430258907617	0.0
26-27	0.0	0.0	0.0	0.0017320166158127344	0.0
28-29	0.0	0.0	0.0	0.0033196985136410744	0.0
30-31	0.0	0.0	0.0	0.0062063928733289645	0.0
32-33	0.0	0.0	0.0	0.011546777438751564	0.0
34-35	0.0	0.0	0.0	0.020928534107737207	0.0
36-37	0.0	0.0	0.0	0.034495997598270295	0.0
38-39	0.0	0.0	0.0	0.059898907963523734	0.0
40-41	0.0	0.0	0.0	0.09251855422799689	0.0
42-43	0.0	0.0	0.0	0.12268451028673535	0.0
44-45	0.0	0.0	0.0	0.1577578467569432	0.0
46-47	0.0	0.0	0.0	0.20120259687024597	0.0
48-49	0.0	0.0	0.0	0.2449360164195175	0.0
50-51	0.0	0.0	0.0	0.2980511926377747	0.0
52-53	0.0	0.0	0.0	0.34972302167618796	0.0
54-55	0.0	0.0	0.0	0.4117869504094776	0.0
56-57	0.0	0.0	0.0	0.48698533847934716	0.0
58-59	0.0	0.0	0.0	0.5652147556268889	0.0
60-61	0.0	0.0	0.0	0.6467638712880719	0.0
62-63	0.0	0.0	0.0	0.7356740575664589	0.0
64-65	0.0	0.0	0.0	0.8338216657958473	0.0
66-67	0.0	0.0	0.0	0.955207163620723	0.0
68-69	0.0	0.0	0.0	1.0881394388843504	0.0
70-71	0.0	0.0	0.0	1.236515528972308	0.0
72-73	0.0	0.0	0.0	1.3783965567509677	0.0
74-75	0.0	0.0	0.0	1.530669684224504	0.0
76-77	0.0	0.0	0.0	1.6957886015986512	0.0
78-79	0.0	0.0	0.0	1.890640470877584	0.0
80-81	0.0	0.0	0.0	2.118977994728896	0.0
82-83	0.0	0.0	0.0	2.3467381797082707	0.0
84-85	0.0	0.0	0.0	2.590952522537866	0.0
86-87	0.0	0.0	0.0	2.8539303787054333	0.0
88-89	0.0	0.0	0.0	3.139280116160581	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGT	50	4.251051E-7	56.965446	6
GGTATCA	335	0.0	45.384933	1
CCGTGCG	65	2.5718018E-6	43.87657	9
GTATCAA	550	0.0	38.87374	1
GTCTAAT	200	0.0	33.258648	1
TCAACGC	665	0.0	30.695665	4
ATCAACG	670	0.0	30.466593	3
TACCGTT	65	0.005855839	29.213049	5
ACCGTGC	100	4.958852E-5	28.486834	8
CGCAGAG	735	0.0	27.77628	8
CGTTGTC	70	0.008402535	27.130318	8
ACGCAGA	760	0.0	26.85871	7
CTAATGT	245	1.0913936E-11	25.1888	3
CAACGCA	815	0.0	25.046156	5
TCTAATG	235	1.7644197E-10	24.240616	2
GTATAAT	275	1.8189894E-12	24.188107	1
AACGCAG	875	0.0	23.871233	6
GTATTGG	120	1.7044456E-4	23.756178	1
AACCGTG	120	1.7144917E-4	23.735603	7
TAGGACC	120	1.7144917E-4	23.735603	4
>>END_MODULE