FastQCFastQC Report
Fri 10 Feb 2017
SRR3224475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346417
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC3930.11344708833573411No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG3510.10132297202504496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.005024090456.983465
TACGTCG250.005024090456.983465
GTATCAA5500.049.4556921
GGTATCA2350.046.705081
ATCAACG6050.043.169293
TCAACGC6400.040.8084684
TCGCACA608.292923E-539.5718469
CAACGCA6950.037.5790185
AACGCAG7550.035.2215656
CCCCTAT851.5780997E-533.6849941
CCGTGCG851.6321654E-533.519689
GTACATG12500.033.2134061
TATCAAC8050.032.4439962
CGCAGAG8350.031.8470428
ACATGGG12400.031.785133
TACATGG12850.031.0415732
GTCTAAT2350.030.4598351
ACGCAGA8600.030.3690917
AAACCGT953.490789E-529.9912936
GTATTAC804.3671622E-429.8252581
Produced by FastQC (version 0.11.5)