##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224475_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346417
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.165693369551725	34.0	33.0	34.0	31.0	34.0
2	33.300421168707075	34.0	34.0	34.0	31.0	34.0
3	33.416884852648685	34.0	34.0	34.0	31.0	34.0
4	36.62719785691811	37.0	37.0	37.0	35.0	37.0
5	36.66513768088748	37.0	37.0	37.0	35.0	37.0
6	36.70323338635229	37.0	37.0	37.0	36.0	37.0
7	36.679845388650094	37.0	37.0	37.0	36.0	37.0
8	36.66406671728004	37.0	37.0	37.0	35.0	37.0
9	38.509484234318755	39.0	39.0	39.0	37.0	39.0
10-11	38.53903676782606	39.0	39.0	39.0	37.5	39.0
12-13	38.54109353755734	39.0	39.0	39.0	37.5	39.0
14-15	40.159472543206604	41.0	40.0	41.0	38.0	41.0
16-17	40.16565728587223	41.0	40.0	41.0	38.0	41.0
18-19	40.16652906756886	41.0	40.0	41.0	38.5	41.0
20-21	40.17870225768366	41.0	40.0	41.0	39.0	41.0
22-23	40.10858156499248	41.0	40.0	41.0	38.0	41.0
24-25	40.08160973624273	41.0	40.0	41.0	38.0	41.0
26-27	40.033996599474044	41.0	40.0	41.0	38.0	41.0
28-29	40.00308010288178	41.0	40.0	41.0	38.0	41.0
30-31	39.941778261459454	41.0	40.0	41.0	38.0	41.0
32-33	39.8971268731038	41.0	40.0	41.0	38.0	41.0
34-35	39.819561395658994	41.0	40.0	41.0	38.0	41.0
36-37	39.73450350300361	41.0	40.0	41.0	37.5	41.0
38-39	39.64756059893135	41.0	40.0	41.0	37.0	41.0
40-41	39.541203231943	41.0	40.0	41.0	37.0	41.0
42-43	39.47096418478308	41.0	40.0	41.0	36.5	41.0
44-45	39.27249817416582	41.0	39.0	41.0	36.0	41.0
46-47	39.07673122277487	41.0	39.0	41.0	35.0	41.0
48-49	39.014316560676875	41.0	39.0	41.0	35.0	41.0
50-51	38.917097024684125	41.0	39.0	41.0	35.0	41.0
52-53	38.74437022432502	40.0	38.5	41.0	35.0	41.0
54-55	38.597990283386785	40.0	38.0	41.0	35.0	41.0
56-57	38.43729522511886	40.0	37.5	41.0	35.0	41.0
58-59	38.218918240155645	40.0	37.0	41.0	35.0	41.0
60-61	38.01955446759253	40.0	36.0	41.0	34.0	41.0
62-63	37.826967210038774	40.0	36.0	41.0	34.0	41.0
64-65	37.593645808375456	39.0	35.0	41.0	34.0	41.0
66-67	37.323506063501505	39.0	35.0	41.0	34.0	41.0
68-69	37.074023503465476	38.5	35.0	41.0	33.5	41.0
70-71	36.78499178735454	37.5	35.0	40.0	33.0	41.0
72-73	36.474120785065395	37.0	35.0	40.0	33.0	41.0
74-75	36.133372496153484	36.5	35.0	39.0	33.0	41.0
76-77	34.19346336929192	34.5	33.0	37.5	30.0	39.0
78-79	35.466465271623505	36.0	35.0	39.0	32.5	39.5
80-81	35.32404154530523	35.5	35.0	37.0	33.0	39.0
82-83	35.036487816706455	35.0	35.0	37.0	33.0	39.0
84-85	34.75851069664595	35.0	35.0	36.5	33.0	38.0
86-87	34.49394804527492	35.0	35.0	36.0	33.0	37.0
88-89	34.27653232953348	35.0	35.0	36.0	32.0	37.0
90-91	34.089654953423185	35.0	35.0	35.5	32.0	36.5
92-93	33.96694157619285	35.0	35.0	35.0	32.0	36.0
94-95	33.83089311436794	35.0	34.5	35.0	32.0	36.0
96-97	33.76494513837369	35.0	34.0	35.0	32.0	36.0
98-99	33.68893270249439	35.0	34.0	35.0	32.0	36.0
100-101	32.914439822526035	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	13.0
16	43.0
17	98.0
18	189.0
19	317.0
20	435.0
21	676.0
22	966.0
23	905.0
24	830.0
25	857.0
26	1000.0
27	1336.0
28	1658.0
29	1909.0
30	2166.0
31	2484.0
32	3127.0
33	4289.0
34	6989.0
35	13280.0
36	33125.0
37	75160.0
38	132329.0
39	62212.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11396451008487	21.101939241358107	13.648941045438463	23.135155203118565
2	14.681150174500674	21.108375166345763	41.46245709650508	22.748017562648485
3	17.696879772066612	28.37793757234778	30.313466140518507	23.6117165150671
4	11.791569120453095	17.975445777776493	41.24797570558027	28.985009396190144
5	12.425487201840555	39.13000805387726	36.19828126217824	12.246223482103938
6	28.028070215953605	39.58206439060439	19.741814056469515	12.648051336972493
7	25.040918892548575	32.586449279336755	25.166490097195	17.206141730919672
8	24.004884286856594	36.89281992511915	21.911164867774964	17.191130920249297
9	25.313134170667144	16.449827808681444	22.05723160237517	36.179806418276236
10-11	23.90139629406178	26.841061495249946	29.913803306419716	19.343738904268555
12-13	24.419846601061725	24.796271545565027	29.997373108132685	20.78650874524056
14-15	21.995167673641884	26.591795437290894	26.76831679738581	24.644720091681414
16-17	21.42850957083515	29.274111836312883	27.997182586304948	21.300196006547022
18-19	21.48667646218286	28.523571302794032	29.125158407352984	20.86459382767012
20-21	22.308487268087514	27.539237846154734	28.940311131510654	21.211963754247098
22-23	22.12474705048598	27.905274917511758	28.29281811017485	21.677159921827414
24-25	21.82286351833703	27.76816346796414	28.484930712018624	21.924042301680203
26-27	21.73523956611794	28.250398735593198	28.479031198804876	21.53533049948399
28-29	21.65039224601439	27.925895453987774	28.840438507227862	21.583273792769972
30-31	21.771461644936917	27.75042615133027	28.61846140966963	21.85965079406318
32-33	21.861288162209597	27.809916401090018	28.84234561913999	21.48644981756039
34-35	21.987892679603465	27.82908297296205	28.541116968146834	21.641907379287648
36-37	21.74783458401352	27.663919933374036	28.72956007522845	21.858685407383994
38-39	21.78516065897459	27.996172243279055	28.31832733382022	21.900339763926134
40-41	21.867142778789724	27.7414214660366	28.784528472909816	21.60690728226386
42-43	21.989971623794442	27.674738826327804	28.62503860953706	21.71025094034069
44-45	22.01192588112515	27.8296541898643	28.26543529754799	21.892984631462557
46-47	21.97914108657027	27.615814638797005	28.121975529778844	22.28306874485389
48-49	21.885951491567894	27.61334703562789	28.358697974100355	22.142003498703865
50-51	21.86512209273794	27.782123856508196	28.13502224198004	22.21773180877382
52-53	22.1540801981427	27.880560134173553	27.89730296145974	22.068056706224002
54-55	22.046839502680296	27.520589347520474	28.13949661823756	22.293074531561675
56-57	22.201660851904727	27.935396238616466	27.573086288950122	22.289856620528685
58-59	22.33123820497604	27.601691697025093	28.132043623720143	21.935026474278725
60-61	22.216912279587252	27.89981822670696	28.003771182219424	21.879498311486365
62-63	21.91592791346845	27.729441684443895	28.229705816977802	22.124924585109852
64-65	22.281960758276874	27.613252236466458	27.825424271903515	22.279362733353157
66-67	22.016962216057525	27.715585551517393	27.779237162148508	22.488215070276574
68-69	22.16028659101603	27.67315114442998	28.12636215890098	22.040200105653014
70-71	22.378809354044403	27.29586019161877	28.05390613047281	22.271424323864014
72-73	21.92415499239356	27.620757641801642	27.971346671785742	22.48374069401906
74-75	22.157544231374327	27.510052913107614	27.877962109249832	22.454440746268226
76-77	21.95444770001299	27.308719310653405	28.167082834172884	22.56975015516072
78-79	22.142510710532697	27.308249708419456	27.637360416642608	22.91187916440523
80-81	21.84352856777874	27.866412814254225	27.60717157122422	22.682887046742813
82-83	21.921458139682574	27.83331505077739	27.797375394758873	22.44785141478116
84-85	22.095525671207053	27.474419725786962	27.97411733800603	22.45593726499995
86-87	21.482923759515266	28.04827707647142	28.002811640306334	22.465987523706975
88-89	22.14715213167945	27.20478498457062	27.73781309808699	22.910249785662945
90-91	21.99372432646204	27.581209929073918	27.66665608212068	22.75840966234336
92-93	22.12579059341776	27.425761437804724	28.037018968468637	22.41142900030888
94-95	21.980156862971505	27.36008914112183	27.74705052003799	22.91270347586868
96-97	22.07974781838074	27.61772661272397	27.9273245828005	22.375200986094793
98-99	21.361971265844343	27.69855405479523	27.984336796404335	22.955137882956087
100-101	21.62939403262312	27.59835974411084	27.784253485688204	22.987992737577834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	2.0
2	1.0
3	2.0
4	4.0
5	3.5
6	5.5
7	10.5
8	19.5
9	28.0
10	26.5
11	27.5
12	28.5
13	32.0
14	38.5
15	45.0
16	49.0
17	52.0
18	59.5
19	74.5
20	92.0
21	115.5
22	125.0
23	155.5
24	207.5
25	256.0
26	383.0
27	556.5
28	823.5
29	1444.5
30	2466.0
31	3966.5
32	6222.0
33	9005.5
34	11979.0
35	14670.5
36	17318.5
37	19071.0
38	18752.0
39	17635.0
40	15759.5
41	13628.5
42	11995.0
43	10806.5
44	10027.0
45	9589.5
46	9502.5
47	9492.0
48	9571.0
49	9900.5
50	10648.5
51	10481.5
52	9817.0
53	10141.5
54	10462.0
55	9887.0
56	8455.5
57	7255.0
58	6732.5
59	6342.0
60	5333.0
61	3997.0
62	3206.5
63	2321.0
64	1455.0
65	975.5
66	757.5
67	659.5
68	507.0
69	320.5
70	193.0
71	126.0
72	96.0
73	72.0
74	47.0
75	33.0
76	25.0
77	17.0
78	9.0
79	3.0
80	3.5
81	4.0
82	2.5
83	1.5
84	0.5
85	0.5
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4742838832967204
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0011546777438751561
22-23	0.0011546777438751561
24-25	1.4433471798439452E-4
26-27	0.002742359641703496
28-29	0.004185706821547442
30-31	7.216735899219726E-4
32-33	2.8866943596878903E-4
34-35	0.004330041539531836
36-37	0.00158768189782834
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.007938409489141698
46-47	0.08140478094319852
48-49	8.660083079063672E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.008227078925110489
58-59	0.03969204744570849
60-61	0.030743294930676034
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	5.773388719375781E-4
78-79	0.008371413643094884
80-81	0.0062063928733289645
82-83	8.660083079063672E-4
84-85	0.002165020769765918
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.07260036314615045
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	346417.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.19113312775012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9906842526171	48.33529475953967
2	12.326947872943194	15.085998167042062
3	3.786948487066498	6.95183007060054
4	1.6280681180115706	3.9849333180116644
5	0.8798185960082194	2.6918548418304584
6	0.5681018452264052	2.085767738482167
7	0.3463388041498606	1.4835004710427926
8	0.25158720602875856	1.2315924973875572
9	0.18828795602401743	1.036939804507585
>10	0.9210481388362293	10.546564637040516
>50	0.07856543473688117	3.262444418804765
>100	0.03360328835130334	3.303279275710236
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAATGTTATTAAATATGTTAAATAAACTTGATGGCGGTGCCAGCGTC	393	0.11344708833573411	No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	351	0.10132297202504496	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.8866943596878903E-4	0.0
5	0.0	0.0	0.0	2.8866943596878903E-4	0.0
6	0.0	0.0	0.0	2.8866943596878903E-4	0.0
7	0.0	0.0	0.0	2.8866943596878903E-4	0.0
8	0.0	0.0	0.0	2.8866943596878903E-4	0.0
9	0.0	0.0	0.0	2.8866943596878903E-4	0.0
10-11	0.0	0.0	0.0	2.8866943596878903E-4	0.0
12-13	0.0	0.0	0.0	2.8866943596878903E-4	0.0
14-15	0.0	0.0	0.0	2.8866943596878903E-4	0.0
16-17	0.0	0.0	0.0	2.8866943596878903E-4	0.0
18-19	0.0	0.0	0.0	2.8866943596878903E-4	0.0
20-21	0.0	0.0	0.0	4.3300415395318355E-4	0.0
22-23	0.0	0.0	0.0	7.216735899219726E-4	0.0
24-25	0.0	0.0	0.0	0.0011546777438751561	0.0
26-27	0.0	0.0	0.0	0.0017320166158127344	0.0
28-29	0.0	0.0	0.0	0.0033196985136410744	0.0
30-31	0.0	0.0	0.0	0.0062063928733289645	0.0
32-33	0.0	0.0	0.0	0.011402442720767167	0.0
34-35	0.0	0.0	0.0	0.02063986467176842	0.0
36-37	0.0	0.0	0.0	0.033629989290363926	0.0
38-39	0.0	0.0	0.0	0.059032899655617366	0.0
40-41	0.0	0.0	0.0	0.09222988479202811	0.0
42-43	0.0	0.0	0.0	0.12254017556875096	0.0
44-45	0.0	0.0	0.0	0.1581908509108964	0.0
46-47	0.0	0.0	0.0	0.20163560102419917	0.0
48-49	0.0	0.0	0.0	0.24580202472742388	0.0
50-51	0.0	0.0	0.0	0.299638874535603	0.0
52-53	0.0	0.0	0.0	0.3514550382920007	0.0
54-55	0.0	0.0	0.0	0.41279729343536836	0.0
56-57	0.0	0.0	0.0	0.48929469396709746	0.0
58-59	0.0	0.0	0.0	0.5683901194225457	0.0
60-61	0.0	0.0	0.0	0.651526916981557	0.0
62-63	0.0	0.0	0.0	0.7423134545937411	0.0
64-65	0.0	0.0	0.0	0.8421930794389421	0.0
66-67	0.0	0.0	0.0	0.9651662591616463	0.0
68-69	0.0	0.0	0.0	1.0991088774511644	0.0
70-71	0.0	0.0	0.0	1.2493613188729191	0.0
72-73	0.0	0.0	0.0	1.3941290410112668	2.8866943596878903E-4
74-75	0.0	0.0	0.0	1.549000193408522	2.8866943596878903E-4
76-77	0.0	0.0	0.0	1.7144077802186382	2.8866943596878903E-4
78-79	0.0	0.0	0.0	1.9105586619594304	2.8866943596878903E-4
80-81	0.0	0.0	0.0	2.140483867708571	2.8866943596878903E-4
82-83	0.0	0.0	0.0	2.3722854247915084	2.8866943596878903E-4
84-85	0.0	0.0	0.0	2.6180874495189324	2.8866943596878903E-4
86-87	0.0	0.0	0.0	2.88207564871239	2.8866943596878903E-4
88-89	0.0	0.0	0.0	3.169301737501335	2.8866943596878903E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	25	0.0050240904	56.98346	5
TACGTCG	25	0.0050240904	56.98346	5
GTATCAA	550	0.0	49.455692	1
GGTATCA	235	0.0	46.70508	1
ATCAACG	605	0.0	43.16929	3
TCAACGC	640	0.0	40.808468	4
TCGCACA	60	8.292923E-5	39.571846	9
CAACGCA	695	0.0	37.579018	5
AACGCAG	755	0.0	35.221565	6
CCCCTAT	85	1.5780997E-5	33.684994	1
CCGTGCG	85	1.6321654E-5	33.51968	9
GTACATG	1250	0.0	33.213406	1
TATCAAC	805	0.0	32.443996	2
CGCAGAG	835	0.0	31.847042	8
ACATGGG	1240	0.0	31.78513	3
TACATGG	1285	0.0	31.041573	2
GTCTAAT	235	0.0	30.459835	1
ACGCAGA	860	0.0	30.369091	7
AAACCGT	95	3.490789E-5	29.991293	6
GTATTAC	80	4.3671622E-4	29.825258	1
>>END_MODULE