FastQCFastQC Report
Fri 10 Feb 2017
SRR3224474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829382
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG18780.2264336578319761No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA17900.2158233479868143No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA16290.1964113038382796No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA12120.1461329037765469No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT10710.12913229368373078No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA9120.10996139294076794No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC9000.10851453250733678No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA8990.10839396080455085No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC8740.10537966823490261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.045.332721
GGTATCA5200.043.914881
ACCGTGC1502.5465852E-1134.8206258
ATGCGGG2350.034.3491638
AATGCGG2450.032.9471557
ATCAACG13000.031.7768613
AAATGCG2550.031.6551116
TCAACGC13200.031.2953934
GTATTAC1551.3333192E-930.6931931
CCGTGCG1503.3265678E-828.5067869
CGCTCGA856.347261E-427.984971
AACCGTG1554.600406E-827.5705837
AAACCGT1554.600406E-827.5705836
TATAACG1056.920729E-527.1329542
ACATGGG28650.026.5173723
TCGCACC7600.026.2911431
CAGTGCG1457.866747E-726.2131399
TACATGG29900.026.0440042
GTACATG29700.025.94971
CGCAGAG16450.025.6897098
Produced by FastQC (version 0.11.5)