##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224474_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	829382
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06322900665797	34.0	31.0	34.0	30.0	34.0
2	32.23572250181461	34.0	31.0	34.0	31.0	34.0
3	32.37899303336701	34.0	31.0	34.0	31.0	34.0
4	35.683967098393744	37.0	35.0	37.0	35.0	37.0
5	35.68167864747487	37.0	37.0	37.0	35.0	37.0
6	35.78343875319214	37.0	37.0	37.0	35.0	37.0
7	35.74714184778546	37.0	37.0	37.0	35.0	37.0
8	35.78721385320636	37.0	37.0	37.0	35.0	37.0
9	37.49538933808547	39.0	38.0	39.0	35.0	39.0
10-11	37.564536003916174	39.0	38.0	39.0	35.0	39.0
12-13	37.56849256434309	39.0	38.0	39.0	35.0	39.0
14-15	39.03544024345838	41.0	39.5	41.0	36.0	41.0
16-17	38.86519058769059	41.0	39.5	41.0	35.5	41.0
18-19	38.94372376058318	41.0	39.5	41.0	36.0	41.0
20-21	39.01075258445445	41.0	40.0	41.0	36.0	41.0
22-23	38.963508974151836	41.0	39.0	41.0	36.0	41.0
24-25	38.899133933458884	41.0	39.0	41.0	35.0	41.0
26-27	38.724341135930125	41.0	39.0	41.0	35.0	41.0
28-29	38.63795452517658	41.0	39.0	41.0	35.0	41.0
30-31	38.569733247164756	40.0	39.0	41.0	35.0	41.0
32-33	38.38666983368339	40.0	38.5	41.0	34.0	41.0
34-35	38.38872377264035	40.0	38.0	41.0	34.0	41.0
36-37	38.39713364890967	40.0	38.0	41.0	34.0	41.0
38-39	38.29328644701718	40.0	38.0	41.0	34.0	41.0
40-41	38.201790610358074	40.0	38.0	41.0	34.0	41.0
42-43	38.06320368660039	40.0	38.0	41.0	33.0	41.0
44-45	37.89984470364681	40.0	38.0	41.0	33.0	41.0
46-47	37.73096413956416	40.0	37.5	41.0	33.0	41.0
48-49	37.53258088552681	40.0	37.0	41.0	32.0	41.0
50-51	36.27484379935904	38.5	35.5	40.0	30.5	40.5
52-53	36.636324998613425	39.0	35.5	40.0	31.0	40.5
54-55	37.125752065996124	40.0	36.0	41.0	32.0	41.0
56-57	36.975789202080584	39.0	35.0	41.0	31.0	41.0
58-59	36.8420583036526	39.0	35.0	41.0	31.0	41.0
60-61	36.60788333964325	39.0	35.0	41.0	31.0	41.0
62-63	36.332694705214244	38.5	35.0	41.0	31.0	41.0
64-65	36.090804357943625	37.5	35.0	41.0	30.0	41.0
66-67	35.851023412613245	37.0	35.0	40.0	30.0	41.0
68-69	35.54316527245588	36.5	35.0	40.0	30.0	41.0
70-71	35.250772261756346	36.0	35.0	39.5	29.0	41.0
72-73	34.9343589564278	36.0	34.5	39.0	29.0	41.0
74-75	34.648318868748056	35.0	34.0	39.0	29.0	40.5
76-77	34.29087742439552	35.0	34.0	37.5	29.0	39.5
78-79	34.03939981817787	35.0	34.0	37.0	29.0	39.0
80-81	33.714051546814375	35.0	34.0	36.5	28.5	39.0
82-83	33.43432760778507	35.0	34.0	36.0	28.0	38.0
84-85	33.19302987043365	35.0	34.0	36.0	27.0	37.0
86-87	32.945611310590294	35.0	34.0	35.5	27.0	37.0
88-89	32.77567031838164	35.0	33.0	35.0	27.0	36.0
90-91	32.59309823458913	35.0	33.0	35.0	26.5	36.0
92-93	32.44432782481414	35.0	33.0	35.0	26.0	36.0
94-95	32.300562949280305	35.0	33.0	35.0	25.5	35.5
96-97	32.160165641405285	35.0	33.0	35.0	25.0	35.0
98-99	32.10237260996742	35.0	33.0	35.0	25.0	35.0
100-101	30.888169142807538	34.0	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	125.0
8	462.0
9	398.0
10	493.0
11	1042.0
12	1724.0
13	1978.0
14	2072.0
15	2029.0
16	2126.0
17	2145.0
18	2426.0
19	2657.0
20	3017.0
21	3712.0
22	4281.0
23	4233.0
24	4262.0
25	4609.0
26	4936.0
27	5263.0
28	5804.0
29	6925.0
30	8412.0
31	10664.0
32	13986.0
33	19712.0
34	32126.0
35	55836.0
36	115941.0
37	193333.0
38	241148.0
39	71497.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59182327807955	19.578701008866528	13.53226464812851	24.297211064925403
2	15.774034160374834	20.250861484816404	38.675664530939905	25.29943982386886
3	19.409800091634715	26.1391641949408	28.570136728640673	25.880898984783812
4	12.951691741561788	17.106110332753786	38.758979577564986	31.183218348119446
5	13.918676797904947	38.45923832443916	34.18231888321666	13.439765994439231
6	29.779040297474506	37.3226088822762	19.29834503280756	13.600005787441733
7	26.951875010550026	31.137642244466363	22.931652724558766	18.978830020424848
8	25.618834264548784	34.728146981728564	20.971518552367908	18.681500201354744
9	26.284269492224333	15.928486511643609	20.397838390512455	37.38940560561961
10-11	25.61449368324849	25.339590200896573	28.086756163022585	20.959159952832348
12-13	26.087195044020728	22.961916221958038	28.091699602836812	22.859189131184422
14-15	23.156745738589247	25.0526023778868	25.157173915009995	26.633477968513958
16-17	23.315713651412633	27.538887172903735	26.229778187469233	22.9156209882144
18-19	23.20374825883247	27.169811320754718	26.886762302743055	22.739678117669758
20-21	24.188121660080053	25.72651268483579	26.640959472572323	23.44440618251183
22-23	24.105572838152252	26.331257612033752	26.02795256424873	23.53521698556526
24-25	23.46356487438513	26.376288678442094	26.32533847216913	23.834807975003645
26-27	23.422078125640535	26.946027282964906	26.741899390148326	22.88999520124623
28-29	23.182562438056287	27.10041934838229	26.709345030396126	23.0076731831653
30-31	23.426720136197794	26.39616003240967	26.729058503801628	23.448061327590906
32-33	23.130656320006945	26.555254394235707	26.799834093336965	23.51425519242038
34-35	23.65309350817838	26.179673540057536	26.60354336120147	23.56368959056261
36-37	23.181054578914935	26.23941302672566	27.120680943026017	23.45885145133339
38-39	23.224366904687834	26.59012368901461	26.38532178677451	23.80018761952304
40-41	23.708056604592613	26.065435796520575	27.03181596754979	23.19469163133703
42-43	23.62572669292397	26.39835824521783	26.766474971860717	23.209440089997486
44-45	23.59307698615204	26.126382504594282	26.517137510068828	23.763402999184848
46-47	23.483871123318327	26.04077493844815	26.520047457022216	23.95530648121131
48-49	23.18563701647733	26.355346511016638	26.657499198198174	23.801517274307855
50-51	23.193353605455627	26.726164782934763	26.410568350892593	23.669913260717017
52-53	23.447880470036726	26.481946798941863	25.99290797244213	24.07726475857928
54-55	23.49430057560931	25.994234261172778	26.343831913400578	24.16763324981733
56-57	23.38429299423727	26.40015481415971	26.300321384067527	23.915230807535494
58-59	23.642762566053026	26.439419336322352	26.577253400027494	23.34056469759713
60-61	23.521317924107937	26.526888664186092	26.35233197486625	23.59946143683972
62-63	23.240073617205006	26.304100323944645	26.73025758785461	23.725568470995736
64-65	24.025179385328187	25.795239269901565	26.166585143873156	24.012996200897092
66-67	23.22890098824835	26.179355920144225	26.071969080307987	24.519774011299436
68-69	23.328936485238405	26.333764176217954	26.986539375100975	23.35075996344266
70-71	23.73900084641335	25.91598322606471	26.6530380451951	23.691977882326842
72-73	23.417616972637457	26.244963117116114	26.73550908990067	23.60191082034575
74-75	23.4823639770335	26.006291431451366	26.765410872191588	23.745933719323546
76-77	23.393623203783058	25.694312150492777	26.285776638509155	24.62628800721501
78-79	23.428888067526444	25.27797859620583	26.912536757811772	24.380596578455958
80-81	23.24077212885543	26.18891364640397	26.38754344461347	24.18277078012713
82-83	23.17647044631796	26.474668358699756	26.555068077391216	23.793793117591072
84-85	23.323355315525667	25.71940623959786	26.938235989367502	24.019002455508975
86-87	23.172718548441093	26.639418671913596	26.50780313645491	23.680059643190404
88-89	23.413558954071785	25.8782202817445	26.292695870320337	24.415524893863374
90-91	23.069285323288906	25.866247398665514	26.55205924411188	24.5124080339337
92-93	23.022320933350695	26.012713478892056	26.98587819947043	23.979087388286814
94-95	23.4481216134423	25.564516712443723	26.733881371913064	24.253480302200916
96-97	23.50852803653805	26.146938322751158	26.780241191634254	23.56429244907654
98-99	22.71443444961769	26.110069829817327	26.878420880339192	24.297074840225786
100-101	23.31580061554495	25.739260079503367	26.48031444299092	24.464624861960758
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	1.5
4	1.5
5	2.5
6	3.0
7	6.0
8	10.5
9	14.5
10	15.5
11	16.0
12	22.5
13	28.5
14	29.5
15	29.0
16	33.5
17	44.0
18	53.0
19	54.5
20	65.0
21	80.5
22	96.5
23	130.5
24	174.5
25	220.5
26	297.5
27	383.0
28	539.0
29	796.0
30	1154.5
31	1938.5
32	3735.0
33	6942.0
34	11645.0
35	17993.5
36	25654.5
37	31885.0
38	34629.0
39	35235.0
40	33961.0
41	31672.0
42	29527.0
43	27500.0
44	26192.0
45	26280.0
46	27420.0
47	27588.0
48	28466.0
49	31134.0
50	33415.5
51	33253.5
52	32539.5
53	33006.5
54	33882.0
55	33944.0
56	29568.0
57	25052.0
58	23107.0
59	21219.5
60	17517.5
61	13239.0
62	10615.0
63	7663.0
64	4800.5
65	3148.5
66	2460.0
67	2117.0
68	1645.5
69	1058.0
70	660.5
71	445.5
72	326.5
73	259.0
74	209.0
75	148.0
76	96.5
77	67.5
78	44.5
79	28.0
80	23.0
81	20.0
82	11.5
83	9.0
84	9.5
85	9.0
86	6.0
87	5.0
88	6.0
89	4.5
90	4.5
91	4.0
92	4.0
93	4.0
94	2.0
95	3.0
96	4.0
97	5.0
98	4.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.159636934488571
2	0.0
3	2.4114340557185952E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03430264944259702
16-17	0.06920815739912368
18-19	0.02375262544882816
20-21	0.036352368389957826
22-23	0.061913569380574934
24-25	0.017000610092816094
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.021582334798681428
38-39	0.0053051549225809095
40-41	0.006028585139296488
42-43	0.0030745784210412085
44-45	0.010851453250733679
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	6.028585139296488E-5
58-59	0.014950891145455291
60-61	0.016699180835851275
62-63	0.027490348235191985
64-65	0.04467181588218698
66-67	0.016518323281672378
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.016458037430279415
80-81	0.054618981362026184
82-83	0.04810810941158598
84-85	0.02724920482962013
86-87	0.054558695510633214
88-89	0.0315897861299136
90-91	0.0
92-93	0.0031348642724341735
94-95	0.0
96-97	0.0
98-99	0.001024859473680403
100-101	0.04346609885432768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	829382.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.685680725463115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.08498368509514	40.30988461394625
2	14.368476433853534	15.427628766784036
3	4.812679051394193	7.751158529618202
4	2.139048253631371	4.5934504640325295
5	1.0833572112091496	2.908038467630126
6	0.6232304844780089	2.007513168483725
7	0.3847294374820547	1.4458123222444026
8	0.28240564750647745	1.2128911541680343
9	0.1857631037462824	0.8975536810464599
>10	0.8589745532029127	8.43504583505684
>50	0.08772351738615536	3.3319252145815965
>100	0.08296182939223382	9.052657627890122
>500	0.004533433297995963	1.7040282390637014
>1k	0.0011333583244989907	0.9224119154540353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1878	0.2264336578319761	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1790	0.2158233479868143	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	1629	0.1964113038382796	No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA	1212	0.1461329037765469	No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT	1071	0.12913229368373078	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	912	0.10996139294076794	No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC	900	0.10851453250733678	No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA	899	0.10839396080455085	No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	874	0.10537966823490261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2057170278592976E-4
2	0.0	0.0	0.0	0.0	1.2057170278592976E-4
3	0.0	0.0	0.0	0.0	1.2057170278592976E-4
4	0.0	0.0	0.0	0.0	1.2057170278592976E-4
5	0.0	0.0	0.0	0.0	1.2057170278592976E-4
6	0.0	0.0	0.0	0.0	1.2057170278592976E-4
7	0.0	0.0	0.0	0.0	1.2057170278592976E-4
8	0.0	0.0	0.0	0.0	1.2057170278592976E-4
9	0.0	0.0	0.0	0.0	1.2057170278592976E-4
10-11	0.0	0.0	0.0	1.2057170278592976E-4	1.2057170278592976E-4
12-13	0.0	0.0	0.0	1.2057170278592976E-4	2.4114340557185952E-4
14-15	0.0	0.0	0.0	1.2057170278592976E-4	3.014292569648244E-4
16-17	0.0	0.0	0.0	1.2057170278592976E-4	3.617151083577893E-4
18-19	0.0	0.0	0.0	1.2057170278592976E-4	6.028585139296488E-4
20-21	6.028585139296488E-5	0.0	0.0	2.4114340557185952E-4	8.440019195015084E-4
22-23	1.2057170278592976E-4	0.0	0.0	3.617151083577893E-4	8.440019195015084E-4
24-25	1.2057170278592976E-4	0.0	0.0	4.220009597507542E-4	8.440019195015084E-4
26-27	1.2057170278592976E-4	0.0	0.0	7.234302167155786E-4	8.440019195015084E-4
28-29	1.2057170278592976E-4	0.0	0.0	0.0018085755417889464	8.440019195015084E-4
30-31	1.2057170278592976E-4	0.0	0.0	0.00506401151700905	8.440019195015084E-4
32-33	1.2057170278592976E-4	0.0	0.0	0.013082029752273377	8.440019195015084E-4
34-35	1.2057170278592976E-4	0.0	0.0	0.0248980566252945	9.042877708944732E-4
36-37	1.2057170278592976E-4	0.0	0.0	0.04557610365308146	9.645736222874381E-4
38-39	1.2057170278592976E-4	0.0	0.0	0.07668360297185133	9.645736222874381E-4
40-41	1.2057170278592976E-4	0.0	0.0	0.11562826297170664	9.645736222874381E-4
42-43	1.2057170278592976E-4	0.0	0.0	0.1582503599065328	9.645736222874381E-4
44-45	1.2057170278592976E-4	0.0	0.0	0.20816704485990772	0.0010851453250733678
46-47	1.2057170278592976E-4	0.0	0.0	0.26706632167083444	0.0012057170278592976
48-49	1.2057170278592976E-4	0.0	0.0	0.3285578900916586	0.0013262887306452274
50-51	1.2057170278592976E-4	0.0	0.0	0.3915566047973069	0.0013262887306452274
52-53	1.2057170278592976E-4	0.0	0.0	0.46191019337289696	0.0013262887306452274
54-55	1.2057170278592976E-4	0.0	0.0	0.5302743488525191	0.0014468604334311572
56-57	1.2057170278592976E-4	0.0	0.0	0.6150965417624207	0.001567432136217087
58-59	1.2057170278592976E-4	0.0	0.0	0.7092630416382318	0.001567432136217087
60-61	1.2057170278592976E-4	0.0	0.0	0.8150044249814923	0.0018688613931819114
62-63	1.2057170278592976E-4	0.0	0.0	0.9282815397488733	0.002049718947360806
64-65	1.2057170278592976E-4	0.0	0.0	1.043728945166401	0.002049718947360806
66-67	1.2057170278592976E-4	0.0	0.0	1.173825812472419	0.002049718947360806
68-69	1.2057170278592976E-4	0.0	0.0	1.3280370203356233	0.002049718947360806
70-71	1.2057170278592976E-4	0.0	0.0	1.4906279615424496	0.002110004798753771
72-73	1.2057170278592976E-4	0.0	0.0	1.6703400845448781	0.0022305765015397006
74-75	1.2057170278592976E-4	0.0	0.0	1.8511976387237725	0.0022908623529326656
76-77	1.2057170278592976E-4	0.0	0.0	2.054782958877815	0.0022908623529326656
78-79	1.2057170278592976E-4	0.0	0.0	2.2839897658738675	0.0022908623529326656
80-81	1.2057170278592976E-4	0.0	0.0	2.5292928951918414	0.0022908623529326656
82-83	1.2057170278592976E-4	0.0	0.0	2.7929229233332773	0.002411434055718595
84-85	1.2057170278592976E-4	0.0	0.0	3.06788669153659	0.002411434055718595
86-87	1.2057170278592976E-4	0.0	0.0	3.363287363362118	0.002532005758504525
88-89	1.2057170278592976E-4	0.0	0.0	3.6984766971070027	0.0027128633126834198
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	955	0.0	45.33272	1
GGTATCA	520	0.0	43.91488	1
ACCGTGC	150	2.5465852E-11	34.820625	8
ATGCGGG	235	0.0	34.349163	8
AATGCGG	245	0.0	32.947155	7
ATCAACG	1300	0.0	31.776861	3
AAATGCG	255	0.0	31.655111	6
TCAACGC	1320	0.0	31.295393	4
GTATTAC	155	1.3333192E-9	30.693193	1
CCGTGCG	150	3.3265678E-8	28.506786	9
CGCTCGA	85	6.347261E-4	27.98497	1
AACCGTG	155	4.600406E-8	27.570583	7
AAACCGT	155	4.600406E-8	27.570583	6
TATAACG	105	6.920729E-5	27.132954	2
ACATGGG	2865	0.0	26.517372	3
TCGCACC	760	0.0	26.291143	1
CAGTGCG	145	7.866747E-7	26.213139	9
TACATGG	2990	0.0	26.044004	2
GTACATG	2970	0.0	25.9497	1
CGCAGAG	1645	0.0	25.689709	8
>>END_MODULE