FastQCFastQC Report
Fri 10 Feb 2017
SRR3224474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829382
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG12300.1483031944266936No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT11390.137331169473174No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA10770.12985572390044636No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA10610.12792657665587148No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA10220.12322428024722021No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG10010.1206922744887157No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG9520.11478426105220514No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG9450.11394025913270363No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG9020.10875567591290866No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT8690.10477680972097296No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG8540.10296823417918402No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG8470.10212423225968251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5200.052.3174361
GTATCAA10450.047.043091
AATGCGG1550.042.8936127
AAATGCG1700.039.1088836
CCGTGCG1500.037.9914869
ATCAACG13000.036.895583
TCAACGC13300.036.0633474
ATGCGGG1950.034.0949258
ACCGTGC1753.6379788E-1232.5641338
CGTACAC953.4950248E-529.993283
CGCAGAG17350.027.9188168
AAACCGT2053.092282E-1127.7986496
TATCAAC18300.026.9884872
ACGCAGA17900.026.7956717
CAACGCA17950.026.7210335
AACGCAG18700.025.6493346
GTACATG32050.025.0182081
ACATGGG32800.024.0342483
AACCGTG2602.3646862E-1123.7446787
TACATGG33850.023.7096062
Produced by FastQC (version 0.11.5)