##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224474_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	829382
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.183067633490964	34.0	33.0	34.0	31.0	34.0
2	33.31400006269728	34.0	34.0	34.0	31.0	34.0
3	33.431520095685705	34.0	34.0	34.0	31.0	34.0
4	36.6383970233258	37.0	37.0	37.0	35.0	37.0
5	36.66496740946874	37.0	37.0	37.0	35.0	37.0
6	36.70366610319491	37.0	37.0	37.0	36.0	37.0
7	36.681480909882296	37.0	37.0	37.0	36.0	37.0
8	36.6682288740291	37.0	37.0	37.0	36.0	37.0
9	38.513666802510784	39.0	39.0	39.0	37.0	39.0
10-11	38.540433720529265	39.0	39.0	39.0	37.5	39.0
12-13	38.53475358761102	39.0	39.0	39.0	37.0	39.0
14-15	40.159098581835636	41.0	40.0	41.0	38.0	41.0
16-17	40.16714794871362	41.0	40.0	41.0	38.0	41.0
18-19	40.153598100754536	41.0	40.0	41.0	38.0	41.0
20-21	40.15485807504865	41.0	40.0	41.0	38.5	41.0
22-23	40.08561495185572	41.0	40.0	41.0	38.0	41.0
24-25	40.05416502890104	41.0	40.0	41.0	38.0	41.0
26-27	39.99846753365759	41.0	40.0	41.0	38.0	41.0
28-29	39.97155773817131	41.0	40.0	41.0	38.0	41.0
30-31	39.92154459585571	41.0	40.0	41.0	38.0	41.0
32-33	39.88912105640103	41.0	40.0	41.0	38.0	41.0
34-35	39.824002088301896	41.0	40.0	41.0	38.0	41.0
36-37	39.72799807567562	41.0	40.0	41.0	37.0	41.0
38-39	39.63495409835275	41.0	40.0	41.0	37.0	41.0
40-41	39.51404057478942	41.0	40.0	41.0	36.5	41.0
42-43	39.43391525256155	41.0	39.0	41.0	36.0	41.0
44-45	39.2148708315348	41.0	39.0	41.0	35.0	41.0
46-47	39.016881846965575	41.0	39.0	41.0	35.0	41.0
48-49	38.93703142822005	41.0	39.0	41.0	35.0	41.0
50-51	38.80175660913788	40.0	38.0	41.0	35.0	41.0
52-53	38.583260789358825	40.0	37.5	41.0	35.0	41.0
54-55	38.40258590130965	40.0	37.0	41.0	35.0	41.0
56-57	38.18316167941913	40.0	36.5	41.0	35.0	41.0
58-59	37.93510589812656	40.0	36.0	41.0	34.0	41.0
60-61	37.69098135720331	39.0	35.0	41.0	34.0	41.0
62-63	37.47322403910381	39.0	35.0	41.0	34.0	41.0
64-65	37.218331842263275	38.5	35.0	41.0	34.0	41.0
66-67	36.93972198576772	37.5	35.0	41.0	33.0	41.0
68-69	36.677790210060024	37.0	35.0	40.0	33.0	41.0
70-71	36.37534754793328	37.0	35.0	40.0	33.0	41.0
72-73	36.061453588334444	36.0	35.0	39.0	33.0	41.0
74-75	35.73954884480251	36.0	35.0	39.0	33.0	41.0
76-77	33.88780200197255	34.5	32.5	36.5	29.5	39.0
78-79	35.14160543633693	35.0	35.0	37.0	32.5	39.0
80-81	35.02980532492869	35.0	35.0	37.0	33.0	39.0
82-83	34.797073001343165	35.0	35.0	36.5	33.0	38.0
84-85	34.57066285499323	35.0	35.0	36.0	33.0	37.0
86-87	34.35816366885223	35.0	35.0	36.0	33.0	37.0
88-89	34.19546783026398	35.0	35.0	35.0	32.5	36.5
90-91	34.04814367806391	35.0	35.0	35.0	32.0	36.0
92-93	33.94666631298967	35.0	35.0	35.0	32.0	36.0
94-95	33.845190153632466	35.0	34.5	35.0	32.0	36.0
96-97	33.79471883884627	35.0	34.0	35.0	32.0	35.5
98-99	33.755776590280476	35.0	34.0	35.0	32.0	35.0
100-101	32.946618084308554	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	17.0
15	30.0
16	78.0
17	165.0
18	257.0
19	450.0
20	638.0
21	940.0
22	1351.0
23	1407.0
24	1501.0
25	1911.0
26	2509.0
27	3539.0
28	4628.0
29	5380.0
30	5721.0
31	6415.0
32	7833.0
33	10637.0
34	18263.0
35	36162.0
36	98818.0
37	217076.0
38	294976.0
39	108665.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.955012144964236	19.534554089006537	13.519616691602712	23.99081707442652
2	15.646228155421749	20.364560600543538	39.385952432051816	24.603258811982897
3	19.229257447111223	26.552782674328597	28.760691695744544	25.45726818281564
4	12.956635181376013	17.27515186005966	39.213776040473505	30.554436918090822
5	13.761210154066521	37.774752767723434	35.011490483275495	13.452546594934542
6	29.74298935834151	37.095572365930295	19.5285164134259	13.632921862302291
7	27.09439076324299	30.9025274240338	23.19474018003767	18.80834163268554
8	25.53431350089585	34.67545714761111	20.89592009472113	18.89430925677191
9	26.259793436558788	16.162033899939956	20.631868065619944	36.946304597881316
10-11	25.656332064115205	25.360268247924356	28.257485694167467	20.725913993792968
12-13	26.280592055289357	23.119804866756212	28.121782242681903	22.477820835272528
14-15	23.36902657641473	25.197074448203605	25.35604823832685	26.07785073705482
16-17	23.170987554588837	27.750963970763777	26.38711715470073	22.690931319946657
18-19	22.918570694806494	27.25499227135385	27.36205994342776	22.464377090411897
20-21	24.027370007535794	26.069570459683494	26.906525998492842	22.996533534287867
22-23	24.09481155308568	26.358815078646902	26.33349470050514	23.212878667762276
24-25	23.809684571988026	26.19887602923144	26.4348954220075	23.55654397677304
26-27	23.420217335604153	27.109677613829895	26.71612232286847	22.753982727697476
28-29	23.260852920447988	26.954044158651865	26.998295654249922	22.78680726665023
30-31	23.491896030456576	26.28248294654726	26.902888009380526	23.322733013615636
32-33	23.323538880201017	26.55552755609304	27.155975359937113	22.964958203768834
34-35	23.879460544640114	26.213262113329034	26.633950479589565	23.273326862441294
36-37	23.07006427793727	26.347684438855172	27.03441299207709	23.547838291130464
38-39	23.257738894743316	26.555133822532923	26.561102121820824	23.626025160902937
40-41	23.452944481553736	26.43685298209992	26.976712781323926	23.133489755022417
42-43	23.47030680675491	26.476038785505352	26.98328393912576	23.07037046861398
44-45	23.649959996358373	26.123734549460114	26.77856431843984	23.44774113574167
46-47	23.541670311874974	25.88536327693467	26.73668282222988	23.83628358896048
48-49	23.201539470638167	25.9836913534995	27.041353981788717	23.773415194073618
50-51	23.400314933287678	26.29813523804471	26.76637544581387	23.53517438285374
52-53	23.50876917994362	26.47694307327625	26.36113395275036	23.653153794029773
54-55	23.629521740283728	25.86841768931566	26.70964645965309	23.79241411074752
56-57	23.698681755288202	26.07646603100724	26.465819991619664	23.759032222084894
58-59	23.949654583887416	26.095968687901237	26.625355442376875	23.32902128583447
60-61	23.536708398183922	26.544609868304814	26.522599362358655	23.39608237115261
62-63	23.325198762452043	26.240803393370005	26.850112493398697	23.583885350779255
64-65	23.665391821862546	26.032817206064273	26.44836757971598	23.8534233923572
66-67	23.33978793848914	26.258286290273965	26.39320602569142	24.008719745545477
68-69	23.49592829359692	26.312061269716487	26.73828223906475	23.453728197621842
70-71	23.92347555167583	25.98699995900562	26.663768926743042	23.42575556257551
72-73	23.323450472761646	26.25925086389625	26.797362373429856	23.619936289912246
74-75	23.550064988147803	25.94142385535254	26.72532078101526	23.7831903754844
76-77	23.50277949090795	25.51494884422492	26.674974092046032	24.307297572821092
78-79	23.492662951568064	25.488578875305446	26.638800412627962	24.37995776049853
80-81	23.104957586500028	26.17687665418679	26.628985874224533	24.089179885088654
82-83	23.093576054696708	26.270853843292556	26.908986120246187	23.72658398176455
84-85	23.44303794415838	25.822211852824484	26.84998797270437	23.884762230312763
86-87	23.025819224434578	26.483875946186437	26.75232884243931	23.737975986939674
88-89	23.459334781801388	25.8260970216378	26.178226679624107	24.53634151693671
90-91	23.402304366383646	26.059825267488325	26.18865613191509	24.349214234212944
92-93	23.434617582730276	26.022689183030256	26.732856512439383	23.80983672180009
94-95	23.43859644892221	25.77183975538413	26.448307293864588	24.341256501829072
96-97	23.576229047652348	26.173464097364064	26.57508843934399	23.675218415639595
98-99	22.45135534651102	26.318270712409962	26.880556848352143	24.349817092726873
100-101	23.091345744902263	26.01404279680776	26.432768858508478	24.461842599781498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.0
2	1.0
3	3.0
4	3.0
5	2.0
6	3.5
7	4.5
8	8.5
9	15.5
10	16.5
11	15.0
12	14.5
13	16.0
14	25.5
15	30.5
16	32.0
17	38.0
18	44.0
19	51.5
20	58.0
21	67.5
22	84.0
23	124.0
24	155.0
25	206.5
26	296.0
27	370.0
28	488.5
29	724.5
30	1149.5
31	2059.0
32	3843.0
33	7160.0
34	12067.0
35	18339.5
36	25924.5
37	31691.0
38	34358.0
39	35338.5
40	34239.0
41	31866.0
42	29644.0
43	28094.5
44	27086.5
45	26930.0
46	27284.0
47	27902.0
48	29035.5
49	31726.0
50	34662.5
51	34086.0
52	32017.0
53	32102.5
54	33817.0
55	33404.0
56	28486.5
57	24367.0
58	23154.0
59	21742.5
60	17854.0
61	13046.5
62	10269.0
63	7247.0
64	4223.5
65	2637.5
66	2050.5
67	1742.5
68	1288.0
69	832.0
70	500.5
71	335.5
72	234.5
73	179.5
74	157.0
75	116.0
76	66.5
77	46.5
78	27.5
79	15.5
80	9.5
81	6.0
82	7.0
83	6.0
84	3.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47469079386820545
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.440019195015083E-4
22-23	0.0012660028792522626
24-25	1.2057170278592976E-4
26-27	0.00235114820432563
28-29	0.0037980086377567878
30-31	5.42572662536684E-4
32-33	1.2057170278592976E-4
34-35	0.003255435975220104
36-37	0.0018085755417889464
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.009947165479839206
46-47	0.08060218331239405
48-49	7.234302167155786E-4
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.007174016315762821
58-59	0.037316942012245266
60-61	0.028032920897728672
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	4.2200095975075414E-4
78-79	0.00765630312690654
80-81	0.0056668700309387
82-83	0.001024859473680403
84-85	0.0022305765015397006
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06733929600594177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	829382.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.372875955921685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18847510649171	37.5990245304886
2	15.25727720596233	15.676204186540282
3	5.2606364613717185	8.107620731377441
4	2.3311920600640867	4.790401621244075
5	1.1888118242180612	3.0536341190243714
6	0.7146146001152928	2.2027084324808106
7	0.4394340309290838	1.5802492973211333
8	0.2731651494555166	1.122662347076725
9	0.21297024778328252	0.9846804709505229
>10	0.9333295074626627	8.639879185997486
>50	0.09769912508242155	3.530842794060385
>100	0.09394007437422411	9.531414928702443
>500	0.007045505574705287	2.3928374607014873
>1k	0.0014091011149410576	0.7878398940342214
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1230	0.1483031944266936	No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT	1139	0.137331169473174	No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA	1077	0.12985572390044636	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	1061	0.12792657665587148	No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA	1022	0.12322428024722021	No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG	1001	0.1206922744887157	No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG	952	0.11478426105220514	No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG	945	0.11394025913270363	No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG	902	0.10875567591290866	No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT	869	0.10477680972097296	No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG	854	0.10296823417918402	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG	847	0.10212423225968251	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2057170278592976E-4
2	0.0	0.0	0.0	0.0	1.2057170278592976E-4
3	0.0	0.0	0.0	0.0	1.2057170278592976E-4
4	0.0	0.0	0.0	0.0	1.2057170278592976E-4
5	0.0	0.0	0.0	0.0	1.2057170278592976E-4
6	0.0	0.0	0.0	0.0	2.4114340557185952E-4
7	0.0	0.0	0.0	0.0	2.4114340557185952E-4
8	0.0	0.0	0.0	0.0	2.4114340557185952E-4
9	0.0	0.0	0.0	0.0	2.4114340557185952E-4
10-11	0.0	0.0	0.0	0.0	2.4114340557185952E-4
12-13	0.0	0.0	0.0	0.0	6.631443653226137E-4
14-15	0.0	0.0	0.0	0.0	7.837160681085435E-4
16-17	0.0	0.0	0.0	0.0	8.440019195015084E-4
18-19	0.0	0.0	0.0	0.0	8.440019195015084E-4
20-21	0.0	0.0	0.0	1.2057170278592976E-4	9.042877708944732E-4
22-23	0.0	0.0	0.0	2.4114340557185952E-4	9.645736222874381E-4
24-25	0.0	0.0	0.0	3.014292569648244E-4	9.645736222874381E-4
26-27	0.0	0.0	0.0	6.028585139296488E-4	9.645736222874381E-4
28-29	0.0	0.0	0.0	0.0016880038390030168	9.645736222874381E-4
30-31	0.0	0.0	0.0	0.005003725665616085	9.645736222874381E-4
32-33	0.0	0.0	0.0	0.013323173157845238	9.645736222874381E-4
34-35	0.0	0.0	0.0	0.025259771733652285	9.645736222874381E-4
36-37	0.0	0.0	0.0	0.04587753291004627	9.645736222874381E-4
38-39	0.0	0.0	0.0	0.07662331712045836	9.645736222874381E-4
40-41	0.0	0.0	0.0	0.11598997808006443	9.645736222874381E-4
42-43	0.0	0.0	0.0	0.15867236086628356	9.645736222874381E-4
44-45	0.0	0.0	0.0	0.2092521901849811	9.645736222874381E-4
46-47	0.0	0.0	0.0	0.26821175284730076	0.0011454311764663328
48-49	0.0	0.0	0.0	0.33036646563344757	0.0013262887306452274
50-51	0.0	0.0	0.0	0.39372689544745365	0.0014468604334311572
52-53	0.0	0.0	0.0	0.4653464869022959	0.001567432136217087
54-55	0.0	0.0	0.0	0.5352780745181351	0.001567432136217087
56-57	0.0	0.0	0.0	0.6213662703072891	0.001567432136217087
58-59	0.0	0.0	0.0	0.7173413457248892	0.001567432136217087
60-61	0.0	0.0	0.0	0.8244693036501878	0.001567432136217087
62-63	0.0	0.0	0.0	0.9390727071482139	0.001567432136217087
64-65	0.0	0.0	0.0	1.055665543742208	0.0016880038390030168
66-67	0.0	0.0	0.0	1.1881135592525518	0.0016880038390030168
68-69	0.0	0.0	0.0	1.344133342657545	0.0016880038390030168
70-71	0.0	0.0	0.0	1.5082917160005884	0.0018085755417889464
72-73	0.0	0.0	0.0	1.6912592749782367	0.002110004798753771
74-75	0.0	0.0	0.0	1.8739856905503134	0.0022908623529326656
76-77	0.0	0.0	0.0	2.0805853032740043	0.0022908623529326656
78-79	0.0	0.0	0.0	2.310997827297916	0.0022908623529326656
80-81	0.0	0.0	0.0	2.5593755350369314	0.00235114820432563
82-83	0.0	0.0	0.0	2.8268035718161233	0.002411434055718595
84-85	0.0	0.0	0.0	3.1048419184404774	0.002411434055718595
86-87	0.0	0.0	0.0	3.4038597413495832	0.002532005758504525
88-89	0.0	0.0	0.0	3.742364796921081	0.0026525774612904548
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	520	0.0	52.317436	1
GTATCAA	1045	0.0	47.04309	1
AATGCGG	155	0.0	42.893612	7
AAATGCG	170	0.0	39.108883	6
CCGTGCG	150	0.0	37.991486	9
ATCAACG	1300	0.0	36.89558	3
TCAACGC	1330	0.0	36.063347	4
ATGCGGG	195	0.0	34.094925	8
ACCGTGC	175	3.6379788E-12	32.564133	8
CGTACAC	95	3.4950248E-5	29.99328	3
CGCAGAG	1735	0.0	27.918816	8
AAACCGT	205	3.092282E-11	27.798649	6
TATCAAC	1830	0.0	26.988487	2
ACGCAGA	1790	0.0	26.795671	7
CAACGCA	1795	0.0	26.721033	5
AACGCAG	1870	0.0	25.649334	6
GTACATG	3205	0.0	25.018208	1
ACATGGG	3280	0.0	24.034248	3
AACCGTG	260	2.3646862E-11	23.744678	7
TACATGG	3385	0.0	23.709606	2
>>END_MODULE