FastQCFastQC Report
Fri 10 Feb 2017
SRR3224473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824348
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT74220.9003479113190059No Hit
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG38740.46994715823899613No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT33850.41062755050051675RNA PCR Primer, Index 33 (95% over 22bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT30810.37374992114980565No Hit
GTCTTGTTCCGGGCAAAAGGCCAGCCAGGTCAGGCCCGAGGCGGTTTTCA30010.3640452818469869No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA29160.35373410258774207No Hit
GAACAAGACCGCAAGGAATTAATCGAAATGTTAGCCTCCCGCCCCGGTGA28670.3477900110147656No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG27070.3283807324091282No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG24960.3027847462479438No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA24390.29587019074468546No Hit
TCCTTTCACCGGGCAATGGTCGGGCGACGTTTGCCGCTTCTGAAAACCGC23490.28495247152901443No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT23050.2796149199124642No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG22220.26954635663578974No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG21710.2633596490802428No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG21630.2623891851499609No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT21620.2622678771586757No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA20730.2514714659342899No Hit
ATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGG19850.2407963627011893No Hit
GTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC19490.2364292750149209No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA18920.22951471951166255No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC17220.2088923609931728No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT16760.20331219339405202No Hit
GGTCAGGCCCGAGGCGGTTTTCAGAAGCGGCAAACGTCGCCCGACCATTG16680.20234172946377013No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT16180.19627632989950844No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC16160.196033713916938No Hit
GTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTG15920.19312232212609237No Hit
GGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAGACCGCAAGGAATTAAT15850.19227316618709572No Hit
GTTTTCGACTGACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGAC15790.19154531823938434No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA15540.1885126184572535No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA15310.18572253465769312No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA15270.18523730269255217No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT15000.18196198692785084No Hit
CGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCA14670.17795882321543813No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA14490.17577527937230394No Hit
GAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG14310.1735917355291697No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG14070.1706803437383241No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCCTTCGATTCTGTTTTGCTAC13940.16910333985161605No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG13500.16376578823506577No Hit
GATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATA13250.1607330884529349No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG12940.1569725407230927RNA PCR Primer, Index 33 (96% over 29bp)
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC12680.1538185329496766No Hit
CCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGACGAACAGTGGATT12540.15212022107168333No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT12270.148844905306982No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT12260.14872359731569676RNA PCR Primer, Index 33 (95% over 24bp)
GTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGA12090.1466613614638478No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG11760.1426581977514351No Hit
GTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATC11750.14253688976014983No Hit
CCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGC11700.14193034980372365No Hit
GCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATG11630.14108119386472703No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC11530.1398681139518747No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA11520.13974680596058947No Hit
ACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATGTCCGCCG11500.139504189978019No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT11460.13901895801287806No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA11350.13768457010874047No Hit
CTGCTGGGCGATCTGTTGCGCGAAGTGGTATGGCCGACAGATGTGTCCAC11280.13683541416974385No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT11260.13659279818717338No Hit
CCGTAGCCCCTCCGATGATAGTTTTCGACTGACCATCAAAGTGCGGCAAT11190.13574364224817675No Hit
CGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTC11120.1348944863091801No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT11000.1334387904137573No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG10670.1294356267013446No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC10650.1291930107187741No Hit
CTGCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATGATAGTTTTCG10440.1266455429017842No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC10430.12652423491049897No Hit
GGATATGTCCGCCGTAGCCCCTCCGATGATAGTTTTCGACTGACCATCAA10400.12616031093664326No Hit
GTGGTATGGCCGACAGATGTGTCCACGCTGGATGTTGATGCAATGGTGGT10180.12349153512836812No Hit
GCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAG10160.12324891914579765No Hit
GACCATCAAAGTGCGGCAATTAAGCGAAGGATTTCGTGACGAACAGTGGA10140.12300630316322719No Hit
GTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAC10130.12288499517194194No Hit
TTTCTGCACTGGCGGCCCCACTGCTGGGCGATCTGTTGCGCGAAGTGGTA10070.12215714722423054No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA9940.12058014333752251No Hit
GCTTTAACGGTTCCCGTGCCAGTTGATAGCCATCACCGGGGCGGGAGGCT9760.11839659949438829No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT9760.11839659949438829No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG9750.11827529150310306No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT9630.11681959560768025No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT9500.11524259172097222No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC9490.11512128372968698No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA9380.1137868958255494No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT9330.11318035586912324No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA9290.1126951239039823No Hit
GGATGTTGATGCAATGGTGGTACGCGAAACCACTCACCGTTTCAGCCGCT9170.1112394280085595No Hit
GTCCACGCTGGATGTTGATGCAATGGTGGTACGCGAAACCACTCACCGTT9080.1101476560869924No Hit
CCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCG9050.10978373211313669No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG8960.10869196019156958No Hit
GCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCT8950.10857065220028433No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT8940.10844934420899911No Hit
CGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGCCAT8800.10675103233100584No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC8770.10638710835715014No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC8760.10626580036586492No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT8700.10553795241815349No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA8590.10420356451401594No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA8550.103718332548875No Hit
TCTGAAAACCGCCTCGGGCCTGACCTGGCTGGCCTTTTGCCCGGAACAAG8510.10323310058373407No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC8500.10311179259244882No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC8490.10299048460116358No Hit
GCATCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGAT8460.10262656062730788No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC8410.10202002067088171No Hit
GATGTGTCCACGCTGGATGTTGATGCAATGGTGGTACGCGAAACCACTCA8370.10153478870574077No Hit
CTTGATGGCGGTGCCAGCGTCGGGCTGCTGGCGGAACTCAGCGGCCTGCA8350.10129217272317032No Hit
GCAGGAAGAGGGATATGTCCGCCGTAGCCCCTCCGATGATAGTTTTCGAC8320.10092824874931461No Hit
CTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGC8250.10007909281031797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1700.058.658459
GGTATCA8100.057.7281151
AAACCGT1650.057.5580756
GCGTAAT504.1107705E-757.2568661
ACCGTGC1950.056.0084348
GTATCAA19550.051.9851341
TACTCCG558.2052793E-751.8022657
GACGTGT405.395453E-447.4854136
AACCGTG2100.047.485417
GTGATCA608.303726E-539.571189
ACTCCGG756.934506E-637.9883278
CTATTAA905.498241E-737.110931
ATCAACG29400.034.5642133
TCAACGC30950.032.833214
GTCTATG902.3391238E-531.809371
CTCCGGA1051.9053732E-631.656949
TGCGCGG1500.031.6569412-13
ATACAAA3100.030.783261
AATGCGG3050.029.5810787
AGACGTG650.00585190229.2217945
Produced by FastQC (version 0.11.5)