FastQCFastQC Report
Fri 10 Feb 2017
SRR3224472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817114
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG13890.16998852057362865No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA9890.12103574287063983No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA8700.10647229150400066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.039.4506761
GGTATCA5600.039.0671271
TACGATC550.002585701634.52774
TCAACGC12100.033.3506164
ATCAACG12200.032.6881073
ACTACGC700.00840371527.1338868
CGCAGAG15700.026.9178528
GTACATG27300.026.306071
ACGCAGA16250.025.7098547
TACATGG27950.025.139132
TATCAAC16200.024.9100282
ACATGGG26700.024.8935663
CAACGCA16350.024.6814985
AACGCAG17100.024.1542456
AATGCGG1852.552406E-723.096237
ATGCGGG1903.2950084E-722.4925638
TCGCACC4750.022.0277821
GCAGAGT19300.021.6681149
CATGGGG17050.020.8836884
AAATGCG2056.858063E-720.842946
Produced by FastQC (version 0.11.5)