##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224472_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	817114
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.068286677256786	34.0	31.0	34.0	31.0	34.0
2	32.22970479027406	34.0	31.0	34.0	31.0	34.0
3	32.37053458880891	34.0	31.0	34.0	31.0	34.0
4	35.59563904179833	37.0	37.0	37.0	35.0	37.0
5	35.606805170392384	37.0	37.0	37.0	35.0	37.0
6	35.7102827757204	37.0	37.0	37.0	35.0	37.0
7	35.674413851677976	37.0	37.0	37.0	35.0	37.0
8	35.705371588297346	37.0	37.0	37.0	35.0	37.0
9	37.44327964029499	39.0	39.0	39.0	35.0	39.0
10-11	37.51646955014845	39.0	39.0	39.0	35.0	39.0
12-13	37.533105539741086	39.0	39.0	39.0	35.0	39.0
14-15	39.04941721718144	41.0	40.0	41.0	37.0	41.0
16-17	38.890498143465905	41.0	40.0	41.0	36.5	41.0
18-19	38.97660546753574	41.0	40.0	41.0	36.0	41.0
20-21	39.03757615216482	41.0	40.0	41.0	37.0	41.0
22-23	39.00054521156167	41.0	40.0	41.0	36.0	41.0
24-25	38.9632664475214	41.0	40.0	41.0	36.0	41.0
26-27	38.82073492805166	41.0	39.5	41.0	36.0	41.0
28-29	38.756358353913896	41.0	39.0	41.0	35.0	41.0
30-31	38.69776799810063	41.0	39.0	41.0	35.0	41.0
32-33	38.518949620248826	41.0	39.0	41.0	35.0	41.0
34-35	38.540657729521214	41.0	39.0	41.0	35.0	41.0
36-37	38.55725027352365	41.0	39.0	41.0	35.0	41.0
38-39	38.470138193691454	41.0	39.0	41.0	35.0	41.0
40-41	38.409227476215065	41.0	39.0	41.0	35.0	41.0
42-43	38.29345721649611	40.5	39.0	41.0	34.0	41.0
44-45	38.17036362123278	40.0	38.0	41.0	34.0	41.0
46-47	38.04797494596837	40.0	38.0	41.0	33.5	41.0
48-49	37.89322114172563	40.0	38.0	41.0	33.0	41.0
50-51	36.690008370924986	39.0	36.5	40.0	31.5	40.5
52-53	37.036176714632234	39.5	37.0	40.5	32.5	40.5
54-55	37.53913591004438	40.0	37.0	41.0	33.0	41.0
56-57	37.44010321693179	40.0	36.5	41.0	33.0	41.0
58-59	37.33180682254863	40.0	36.0	41.0	33.0	41.0
60-61	37.12637465518887	40.0	35.5	41.0	32.5	41.0
62-63	36.86398776670085	39.0	35.0	41.0	32.0	41.0
64-65	36.63598286163253	39.0	35.0	41.0	31.0	41.0
66-67	36.405002851499304	39.0	35.0	41.0	31.0	41.0
68-69	36.10805774959185	38.0	35.0	40.5	31.0	41.0
70-71	35.80326918887695	37.0	35.0	40.0	31.0	41.0
72-73	35.45826836891792	37.0	35.0	39.5	30.0	41.0
74-75	35.12194810026508	36.0	35.0	39.0	30.0	41.0
76-77	34.73215059343005	36.0	35.0	39.0	30.0	40.0
78-79	34.41025120117878	35.0	35.0	37.0	29.5	39.0
80-81	34.0344970224473	35.0	34.0	37.0	29.0	39.0
82-83	33.71467628751925	35.0	34.0	36.5	29.0	38.5
84-85	33.43716849790849	35.0	34.0	36.0	29.0	37.0
86-87	33.16641435589159	35.0	34.0	36.0	29.0	37.0
88-89	32.968479918346766	35.0	34.0	35.5	28.0	36.5
90-91	32.767439427056686	35.0	34.0	35.0	27.0	36.0
92-93	32.601365904879856	35.0	34.0	35.0	27.0	36.0
94-95	32.44559816622895	35.0	33.5	35.0	26.5	36.0
96-97	32.28901475191957	35.0	33.0	35.0	25.5	35.0
98-99	32.19974079504206	35.0	33.0	35.0	25.0	35.0
100-101	30.994840377230105	34.5	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	15.0
7	871.0
8	4253.0
9	2832.0
10	1700.0
11	1693.0
12	1749.0
13	1812.0
14	1675.0
15	1626.0
16	1537.0
17	1628.0
18	1735.0
19	1871.0
20	2111.0
21	2476.0
22	2934.0
23	2918.0
24	3190.0
25	3403.0
26	3633.0
27	3993.0
28	4612.0
29	5368.0
30	6535.0
31	8127.0
32	10723.0
33	15092.0
34	24401.0
35	42767.0
36	90335.0
37	176267.0
38	289709.0
39	93519.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84569226696473	18.965521467580473	12.934106931003717	25.25467933445108
2	15.767238762862373	20.35608337657506	38.9701778948296	24.906499965732973
3	19.42306492018832	25.682679586983898	28.725595420940365	26.168660071887416
4	12.949478285771631	17.061633994766947	38.328801121997664	31.660086597463753
5	13.773843062289961	37.97169060865436	34.84311858565635	13.411347743399329
6	29.833046551643953	37.45511642194357	19.32129421353691	13.390542812875559
7	27.14835384046779	31.054418355333528	23.01845274955514	18.77877505464354
8	25.18498030874517	35.3828229598318	21.423693633936022	18.008503097487008
9	26.363028904167596	15.85604946188659	20.088996149864034	37.69192548408178
10-11	24.811042278066463	25.76874218285331	28.82829078929011	20.591924749790113
12-13	25.571523679682397	23.298034301211338	28.692140876303675	22.438301142802594
14-15	22.705716785025402	25.19036259337546	25.641515359088828	26.46240526251031
16-17	22.78284662034182	27.964599277018863	26.635173773286684	22.617380329352628
18-19	22.761084176514405	27.609495205328948	27.247821480444586	22.38159913771206
20-21	23.566937077457634	26.518765105009663	27.184426199402047	22.729871618130666
22-23	23.455077852319402	26.811471735823822	26.691454002591403	23.04199640926537
24-25	23.02194466341234	26.875908852776863	27.017959175679714	23.084187308131078
26-27	22.864373881734984	27.53324505515754	27.090405989861882	22.511975073245594
28-29	22.763531159666826	27.418817937276806	27.18549676054994	22.63215414250643
30-31	22.970723791294727	27.037965326747553	27.151902916851263	22.83940796510646
32-33	22.66066913551842	27.106682788448122	27.334129631850633	22.89851844418282
34-35	23.100999370956806	26.834566535391634	27.123632687727785	22.940801405923775
36-37	22.729267437312828	26.80046784713811	27.534370912687507	22.935893802861553
38-39	22.82795138963257	27.08200108069275	27.06731442957353	23.022733100101153
40-41	23.00539124794233	26.77345131660272	27.488327122077187	22.73283031337776
42-43	22.980096990836355	26.861321461899735	27.360594795539033	22.797986751724878
44-45	22.894293638573444	26.778234996046596	27.21133438920562	23.11613697617433
46-47	22.866270801870975	26.51417060532557	27.287440920116406	23.33211767268704
48-49	22.493373017718458	26.773681518123542	27.43160685045171	23.30133861370629
50-51	22.535533597515155	26.995131646257438	27.265412170150068	23.20392258607734
52-53	22.68159644798645	26.860205552713573	27.023157111492395	23.435040887807578
54-55	22.711885979190175	26.56379648372198	27.27936371179542	23.44495382529243
56-57	22.655053428930007	26.78489585596126	27.16911420506454	23.3909365100442
58-59	22.76756534447684	26.82758899260445	27.27680549537442	23.12804016754429
60-61	22.793173957827776	26.85292232968709	27.06957933655218	23.284324375932954
62-63	22.459272770675785	26.560855952770794	27.43694662843117	23.54292464812225
64-65	22.99146827048815	26.22104868767111	27.021939082863568	23.765543958977172
66-67	22.523604320170488	26.697684154091057	26.806073992742597	23.97263753299586
68-69	22.43805637891408	26.669473292588304	27.630783464730747	23.26168686376687
70-71	22.78892541310025	26.32992458824595	27.286094718729576	23.59505527992422
72-73	22.473302378860232	26.70594311197703	27.31834236104142	23.502412148121316
74-75	22.570963170377695	26.3545233590417	27.42047009352428	23.654043377056322
76-77	22.525987805863075	26.181475289861634	27.12130743078689	24.1712294734884
78-79	22.786791837344218	25.79138995635673	27.377138174302555	24.044680031996492
80-81	22.58063133558163	26.34769255637275	27.141760284490424	23.929915823555202
82-83	22.340390360973696	26.591443405005634	27.246473526962827	23.821692707057842
84-85	22.648994299572088	25.9232667191843	27.584917278607268	23.84282170263635
86-87	22.35954375100644	26.478189551876007	27.39434899288588	23.76791770423167
88-89	22.572468314719536	25.951905095660315	27.260328896424866	24.21529769319528
90-91	22.54611963569343	25.81341159250729	27.407130461600214	24.233338310199066
92-93	22.591379609979857	25.895009827606884	27.56190902793586	23.951701534477397
94-95	22.813585374868133	25.50751792283574	27.6345161140306	24.04438058826553
96-97	22.963136110750764	25.697148745462687	27.591927197429	23.747787946357548
98-99	22.38955387088464	25.699097975600488	27.49959766566537	24.411750487849503
100-101	22.845558268965522	25.35184710162717	27.237364328651424	24.565230300755882
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	2.5
3	2.0
4	1.0
5	2.0
6	3.5
7	9.0
8	15.0
9	19.5
10	16.0
11	12.5
12	14.0
13	19.5
14	25.5
15	21.0
16	21.0
17	28.5
18	35.0
19	44.0
20	56.0
21	65.5
22	76.0
23	94.5
24	109.0
25	131.0
26	181.5
27	234.5
28	287.5
29	343.5
30	489.5
31	892.0
32	1816.5
33	3810.5
34	7378.5
35	12952.5
36	21418.5
37	30381.0
38	37731.0
39	42883.0
40	45255.5
41	45816.0
42	44769.5
43	42718.0
44	39637.5
45	36375.5
46	33525.0
47	29875.0
48	26621.5
49	25548.0
50	25411.0
51	24255.5
52	23335.5
53	23902.0
54	24931.0
55	24905.0
56	22055.5
57	19358.0
58	18450.5
59	17394.0
60	14885.0
61	11791.0
62	9641.0
63	7103.0
64	4633.5
65	3218.0
66	2480.0
67	2033.5
68	1581.5
69	1113.0
70	766.0
71	525.5
72	393.5
73	311.5
74	237.5
75	173.5
76	137.0
77	93.5
78	55.0
79	38.0
80	24.0
81	19.0
82	14.5
83	14.0
84	12.0
85	6.5
86	6.5
87	4.5
88	2.5
89	4.0
90	4.5
91	2.5
92	2.5
93	3.5
94	2.5
95	4.0
96	4.5
97	2.5
98	2.5
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1451449858893618
2	2.447638885149441E-4
3	3.6714583277241606E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.03885626730174737
16-17	0.07752896168710853
18-19	0.026618072876000164
20-21	0.040630805493480714
22-23	0.06951294433824412
24-25	0.018357291638620807
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0220899409384737
38-39	0.005568378463714977
40-41	0.005996715268616129
42-43	0.003243121522823009
44-45	0.011748666648717314
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	6.119097212873602E-5
58-59	0.016643944419016198
60-61	0.01890801038777943
62-63	0.030167149259466855
64-65	0.04870801381447387
66-67	0.0190303923320369
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.01829610066649207
80-81	0.06070144435170613
82-83	0.05311376380774285
84-85	0.030044767315209383
86-87	0.06100739921234981
88-89	0.035674336751053096
90-91	0.0
92-93	0.003426694439209217
94-95	0.0
96-97	0.0
98-99	9.178645819310403E-4
100-101	0.047545385344027885
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	817114.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.169912790244915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68783662872606	44.60924768765544
2	13.43765674383838	15.633346417886502
3	4.495543332099119	7.845160907189183
4	1.916308525161886	4.458859991514791
5	1.0643173311884646	3.09556231681896
6	0.6069040796644781	2.1182134431675927
7	0.38301762298022013	1.559607120812042
8	0.25770606375198357	1.1992591403176145
9	0.18307243171126197	0.9584376648247959
>10	0.8339426980852102	8.68352668728428
>50	0.07488704308842907	3.0217346413172224
>100	0.056480584255971605	5.8669646221761065
>500	0.00211537768052113	0.7791610544505907
>1k	2.1153776805211302E-4	0.1709183045849448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGCACCACTGTGCGGCGACTGCTGGAGACGCTGCAGGAAGAGGGATATG	1389	0.16998852057362865	No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA	989	0.12103574287063983	No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA	870	0.10647229150400066	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2238194425747204E-4	0.0
10-11	0.0	0.0	0.0	2.447638885149441E-4	0.0
12-13	0.0	0.0	0.0	2.447638885149441E-4	0.0
14-15	0.0	0.0	0.0	2.447638885149441E-4	0.0
16-17	0.0	0.0	0.0	3.671458327724161E-4	0.0
18-19	0.0	0.0	0.0	4.283368049011521E-4	0.0
20-21	0.0	0.0	0.0	9.178645819310403E-4	0.0
22-23	0.0	0.0	0.0	0.0018969201359908166	0.0
24-25	0.0	0.0	0.0	0.004895277770298881	0.0
26-27	0.0	0.0	0.0	0.008566736098023041	0.0
28-29	0.0	0.0	0.0	0.01578727080921389	0.0
30-31	0.0	0.0	0.0	0.03836673952471748	0.0
32-33	0.0	0.0	0.0	0.10023081234686959	0.0
34-35	0.0	0.0	0.0	0.17115114904407464	0.0
36-37	0.0	0.0	0.0	0.2872916141444156	0.0
38-39	0.0	0.0	0.0	0.4850608360644904	0.0
40-41	0.0	0.0	0.0	0.7130583982161608	0.0
42-43	0.0	0.0	0.0	0.9236165333111415	0.0
44-45	0.0	0.0	0.0	1.1440876058909772	0.0
46-47	0.0	0.0	0.0	1.3958272652285972	0.0
48-49	0.0	0.0	0.0	1.6667808898146403	0.0
50-51	0.0	0.0	0.0	1.9466072053593502	0.0
52-53	0.0	0.0	0.0	2.210646250094846	0.0
54-55	0.0	0.0	0.0	2.472298846917321	0.0
56-57	0.0	0.0	0.0	2.7613038082813413	0.0
58-59	0.0	0.0	0.0	3.079190908490125	0.0
60-61	0.0	0.0	0.0	3.4110295503442605	0.0
62-63	0.0	0.0	0.0	3.7426846192820094	0.0
64-65	0.0	0.0	0.0	4.061489584072724	0.0
66-67	0.0	0.0	0.0	4.407952868265628	0.0
68-69	0.0	0.0	0.0	4.790702398930871	0.0
70-71	0.0	0.0	0.0	5.191074929569192	0.0
72-73	0.0	0.0	0.0	5.620329599052274	0.0
74-75	0.0	0.0	0.0	6.041996587991394	0.0
76-77	0.0	0.0	0.0	6.468314090812298	0.0
78-79	0.0	0.0	0.0	6.933610242879206	0.0
80-81	0.0	0.0	0.0	7.436477651833159	0.0
82-83	0.0	0.0	0.0	7.978629664893761	0.0
84-85	0.0	0.0	0.0	8.515641636295548	0.0
86-87	0.0	0.0	0.0	9.042434715351835	0.0
88-89	0.0	0.0	0.0	9.630602339453247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1085	0.0	39.450676	1
GGTATCA	560	0.0	39.067127	1
TACGATC	55	0.0025857016	34.5277	4
TCAACGC	1210	0.0	33.350616	4
ATCAACG	1220	0.0	32.688107	3
ACTACGC	70	0.008403715	27.133886	8
CGCAGAG	1570	0.0	26.917852	8
GTACATG	2730	0.0	26.30607	1
ACGCAGA	1625	0.0	25.709854	7
TACATGG	2795	0.0	25.13913	2
TATCAAC	1620	0.0	24.910028	2
ACATGGG	2670	0.0	24.893566	3
CAACGCA	1635	0.0	24.681498	5
AACGCAG	1710	0.0	24.154245	6
AATGCGG	185	2.552406E-7	23.09623	7
ATGCGGG	190	3.2950084E-7	22.492563	8
TCGCACC	475	0.0	22.027782	1
GCAGAGT	1930	0.0	21.668114	9
CATGGGG	1705	0.0	20.883688	4
AAATGCG	205	6.858063E-7	20.84294	6
>>END_MODULE