FastQCFastQC Report
Fri 10 Feb 2017
SRR3224472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3224472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817114
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT59730.7309873530498804No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT31150.3812197563620254RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG13960.17084519418343094RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT11350.13890350673223076RNA PCR Primer, Index 33 (95% over 24bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGG652.5940699E-643.832347
GGTATCA6150.043.464481
GTATCAA12000.033.81111
ATCAACG11950.032.1865123
TCAACGC12050.031.9194054
TAGTACG600.00395638631.6566934
AAATGCG953.4971326E-529.9905536
TCGCACC3400.028.078421
GTACATG27600.026.2879121
CAACGCA14600.025.6939585
CGCAGAG15100.025.1576398
CGCACCA3800.024.9921262
ATGCGGG1151.2840531E-424.7748038
ACGCAGA15400.024.6675537
AACGCAG15450.024.5877236
TATCAAC15750.024.4208772
ACATGGG27650.023.871323
ACGGACC1000.00165627223.742525
TACATGG29700.023.023052
AAGACCG4750.022.9927565
Produced by FastQC (version 0.11.5)