##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3224472_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	817114
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.180466862640955	34.0	33.0	34.0	31.0	34.0
2	33.31190384695404	34.0	34.0	34.0	31.0	34.0
3	33.42942355656616	34.0	34.0	34.0	31.0	34.0
4	36.63024008889825	37.0	37.0	37.0	35.0	37.0
5	36.662016560724695	37.0	37.0	37.0	35.0	37.0
6	36.70549029878328	37.0	37.0	37.0	36.0	37.0
7	36.686983701172664	37.0	37.0	37.0	36.0	37.0
8	36.67257567487523	37.0	37.0	37.0	36.0	37.0
9	38.534191067586654	39.0	39.0	39.0	38.0	39.0
10-11	38.55657839664967	39.0	39.0	39.0	37.5	39.0
12-13	38.56047381393539	39.0	39.0	39.0	38.0	39.0
14-15	40.190122798042864	41.0	40.0	41.0	38.0	41.0
16-17	40.1997622118823	41.0	40.0	41.0	38.5	41.0
18-19	40.19534361178489	41.0	40.0	41.0	38.5	41.0
20-21	40.20444515697932	41.0	40.0	41.0	39.0	41.0
22-23	40.13389135420517	41.0	40.0	41.0	38.5	41.0
24-25	40.10880427945183	41.0	40.0	41.0	38.0	41.0
26-27	40.05923714438866	41.0	40.0	41.0	38.0	41.0
28-29	40.031337732556295	41.0	40.0	41.0	38.0	41.0
30-31	39.9919399251512	41.0	40.0	41.0	38.0	41.0
32-33	39.965379983698725	41.0	40.0	41.0	38.0	41.0
34-35	39.894452304084865	41.0	40.0	41.0	38.0	41.0
36-37	39.806916782725544	41.0	40.0	41.0	38.0	41.0
38-39	39.718088908034865	41.0	40.0	41.0	37.0	41.0
40-41	39.64394564283564	41.0	40.0	41.0	37.0	41.0
42-43	39.600649970505955	41.0	40.0	41.0	37.0	41.0
44-45	39.423832537442756	41.0	39.5	41.0	36.0	41.0
46-47	39.26457630147078	41.0	39.0	41.0	35.5	41.0
48-49	39.22404095389383	41.0	39.0	41.0	35.0	41.0
50-51	39.106401309976334	41.0	39.0	41.0	35.0	41.0
52-53	38.92919409042068	40.5	38.5	41.0	35.0	41.0
54-55	38.7873889077901	40.0	38.0	41.0	35.0	41.0
56-57	38.59844464787043	40.0	37.5	41.0	35.0	41.0
58-59	38.34947143238276	40.0	37.0	41.0	35.0	41.0
60-61	38.10980230420725	40.0	36.0	41.0	34.5	41.0
62-63	37.874747587239966	39.5	35.5	41.0	34.0	41.0
64-65	37.60109360505389	39.0	35.0	41.0	34.0	41.0
66-67	37.25317275190488	39.0	35.0	41.0	34.0	41.0
68-69	36.90642125823325	38.5	35.0	41.0	33.0	41.0
70-71	36.50530464537384	37.0	35.0	40.0	33.0	41.0
72-73	36.140944225652724	37.0	35.0	39.5	33.0	41.0
74-75	35.77614017138367	36.5	35.0	39.0	33.0	41.0
76-77	33.88209723490235	34.5	33.0	37.0	29.5	39.0
78-79	35.067012681217065	35.0	35.0	37.5	32.5	39.0
80-81	34.902563167440526	35.0	35.0	37.0	33.0	39.0
82-83	34.62379545571365	35.0	35.0	37.0	33.0	39.0
84-85	34.36801963985441	35.0	35.0	36.0	32.5	37.0
86-87	34.12771290174933	35.0	35.0	36.0	32.0	37.0
88-89	33.938813923149034	35.0	35.0	36.0	32.0	36.5
90-91	33.77719510374317	35.0	35.0	35.0	32.0	36.0
92-93	33.671830368834705	35.0	35.0	35.0	32.0	36.0
94-95	33.56253594969613	35.0	35.0	35.0	31.5	36.0
96-97	33.50268689558617	35.0	34.0	35.0	31.0	36.0
98-99	33.44787018702409	35.0	34.0	35.0	31.0	35.0
100-101	32.66725573175836	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	8.0
15	33.0
16	72.0
17	139.0
18	230.0
19	364.0
20	553.0
21	757.0
22	1040.0
23	1206.0
24	1569.0
25	2654.0
26	3782.0
27	5559.0
28	8179.0
29	7009.0
30	5158.0
31	5711.0
32	7185.0
33	9537.0
34	15267.0
35	29533.0
36	76719.0
37	182008.0
38	330178.0
39	122654.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55165841812425	19.229043066613386	13.048845716393595	25.17045279886877
2	15.094221858883827	20.449533357646548	40.38469540357894	24.07154937989069
3	18.698002971433606	26.379060938865322	29.59905227422367	25.323883815477398
4	12.324106550615948	17.854668993555368	38.79740158655952	31.023822869269157
5	13.308057382446023	37.74479448400101	35.78717290365849	13.159975229894483
6	29.3325043996309	37.79974397697261	19.282376755263037	13.585374868133455
7	26.595065070479762	31.375548577065132	23.021145152328806	19.008241200126296
8	24.86091292035138	35.80442875779879	21.548645599022905	17.786012722826925
9	25.8815783354587	15.76891351757527	19.968817080603195	38.38069106636283
10-11	24.447323139733257	25.7648871516092	29.278595153185478	20.509194555472064
12-13	25.345178273778203	23.23880144019072	28.833553212893182	22.582467073137895
14-15	22.66409582995763	25.319600447428385	25.56503743663675	26.451266285977233
16-17	22.629584121677023	28.400382321193863	26.4565287095803	22.513504847548813
18-19	22.57022887871215	27.605756357130097	27.27795631943646	22.5460584447213
20-21	23.212777779137593	26.725479925493147	27.06717365923148	22.99456863613778
22-23	23.212289323393296	27.310627408289932	26.58807158736482	22.889011680951953
24-25	23.253925565849947	27.236368598410376	26.715448258897347	22.794257576842327
26-27	22.84437286285299	28.266086888411863	26.795815414138396	22.093724834596753
28-29	22.704119410145605	27.639113774243253	27.172147749983782	22.48461906562736
30-31	23.319795792613114	27.022722141007854	27.124666737240556	22.53281532913847
32-33	22.82855787911701	27.447077360828526	27.414829659318634	22.309535100735822
34-35	23.15052901472919	26.9544784202378	27.10152554507793	22.793467019955084
36-37	22.38107010288839	27.331449432688082	27.40237107131449	22.885109393109033
38-39	22.716169347239184	26.819880702080738	27.24044625352154	23.223503697158538
40-41	22.519195607956785	26.798953389612713	27.95644181839988	22.725409184030624
42-43	22.93817019412224	26.654236740528248	27.999703835694895	22.407889229654614
44-45	22.481341228480296	26.783445649060884	27.755305112833856	22.979908009624964
46-47	23.0103907937861	26.125056950956044	27.392062628905123	23.47248962635274
48-49	22.55910963652752	26.155773221173057	27.80274738420851	23.48236975809092
50-51	22.213730275090136	26.418835070749005	27.942796231615176	23.42463842254569
52-53	22.378422568237198	26.69875525172161	28.01889090003463	22.90393128000656
54-55	22.343883472807956	26.462464233876794	27.79196048531784	23.401691807997416
56-57	22.770315919338493	26.678244990477868	27.164142934774627	23.38729615540901
58-59	22.313760707462304	27.223047619397427	27.659212381324927	22.803979291815345
60-61	22.522566637918317	27.804203543208523	26.87344258522131	22.79978723365185
62-63	21.99699185181015	27.851438110226965	27.054670462138695	23.09689957582418
64-65	22.198983250807107	27.741294360395248	26.679630993961673	23.380091394835972
66-67	21.95146576854637	27.978225804477713	26.62229505307705	23.448013373898867
68-69	22.204062101493793	27.664560881345814	27.001005979581798	23.130371037578602
70-71	22.59495003145216	27.48386394064965	26.696825657129853	23.224360370768338
72-73	22.208284278570677	27.786453297826252	26.648117643315377	23.357144780287694
74-75	22.31665349021067	27.36937563179679	26.86142937215615	23.452541505836393
76-77	22.38023819270687	27.094772255683587	26.695438749791183	23.829550801818357
78-79	22.5438545494601	26.914897848656288	26.524161691945697	24.017085909937915
80-81	22.154316318712656	27.421419671946563	26.575890201722757	23.848373807618025
82-83	22.07702884374738	27.48358696111109	26.695810047490575	23.74357414765096
84-85	22.457566445274207	26.895512305088353	26.793627715545572	23.85329353409187
86-87	22.026363518431943	27.277895128464323	26.905364490144585	23.790376862959146
88-89	22.462104430960675	26.79546550420137	26.391972233984482	24.35045783085347
90-91	22.555787809289768	26.732561184853033	26.48180058106947	24.229850424787728
92-93	22.48658081981217	26.76070903203225	26.76174927855844	23.990960869597146
94-95	22.425328656711304	26.52347163308914	26.621315997522988	24.429883712676567
96-97	22.639068722356978	26.50480838658988	26.848273313148475	24.007849577904675
98-99	21.814459181950134	26.616175955864176	26.972123840737034	24.59724102144866
100-101	22.20838384951688	26.494360556963887	26.625335243763548	24.671920349755684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	1.5
3	0.5
4	0.5
5	1.5
6	1.5
7	2.0
8	7.0
9	11.0
10	12.5
11	13.0
12	13.0
13	13.0
14	20.5
15	24.0
16	18.0
17	18.5
18	28.5
19	36.0
20	34.5
21	45.5
22	58.5
23	69.5
24	94.0
25	130.5
26	165.0
27	203.0
28	280.5
29	352.0
30	494.5
31	898.0
32	1887.5
33	4021.0
34	7797.5
35	13925.0
36	22908.5
37	32125.0
38	38902.5
39	43767.0
40	46476.0
41	46326.5
42	45114.5
43	43316.0
44	40608.5
45	37585.5
46	33951.0
47	30374.5
48	27512.0
49	26318.5
50	25976.5
51	24422.0
52	22482.0
53	22492.5
54	24009.0
55	24017.5
56	21132.5
57	18573.0
58	17892.5
59	16912.5
60	14114.5
61	10866.0
62	8648.0
63	6132.5
64	3826.5
65	2531.0
66	1871.5
67	1491.5
68	1133.0
69	762.0
70	512.5
71	376.0
72	289.5
73	221.5
74	161.5
75	110.5
76	73.5
77	41.0
78	28.5
79	17.5
80	9.5
81	7.0
82	5.0
83	2.5
84	1.5
85	1.5
86	1.0
87	0.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4698242840044351
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	9.790555540597763E-4
22-23	0.0017745381917333445
24-25	3.059548606436801E-4
26-27	0.002447638885149441
28-29	0.003793840271981633
30-31	4.283368049011521E-4
32-33	1.8357291638620803E-4
34-35	0.003549076383466689
36-37	0.0018357291638620805
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.008811499986537987
46-47	0.0761215693281476
48-49	0.0011626284704459844
50-51	0.0
52-53	1.2238194425747204E-4
54-55	0.0
56-57	0.008566736098023043
58-59	0.04105914229838187
60-61	0.03126858675778411
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	3.059548606436801E-4
78-79	0.00813839929312189
80-81	0.006363861101388546
82-83	5.507187491586242E-4
84-85	0.0015909652753471365
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06657577767606479
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	817114.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.19951898269658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.04549085296551	41.424749969040526
2	14.288341397024972	15.774191443526586
3	4.836936825933829	8.009897584237157
4	2.1162241289630166	4.672582159133383
5	1.0912037078948529	3.0116959893965416
6	0.6452010382715966	2.136887217583712
7	0.4096492185889171	1.5828707872422028
8	0.28592639578633583	1.262639960948949
9	0.20179663652424795	1.0025169541618093
>10	0.9308647246824054	9.142852187968572
>50	0.0793950694782281	3.0774535859215697
>100	0.06519993937005401	6.4925320919540646
>500	0.002882990512282567	0.986788490105766
>1k	6.653055028344385E-4	0.6911559130550993
>5k	2.2176850094481285E-4	0.7311856657240714
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	5973	0.7309873530498804	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	3115	0.3812197563620254	RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG	1396	0.17084519418343094	RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT	1135	0.13890350673223076	RNA PCR Primer, Index 33 (95% over 24bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2238194425747204E-4	0.0
3	0.0	0.0	0.0	1.2238194425747204E-4	0.0
4	0.0	0.0	0.0	1.2238194425747204E-4	0.0
5	0.0	0.0	0.0	1.2238194425747204E-4	0.0
6	0.0	0.0	0.0	1.2238194425747204E-4	0.0
7	0.0	0.0	0.0	1.2238194425747204E-4	0.0
8	0.0	0.0	0.0	1.2238194425747204E-4	0.0
9	0.0	0.0	0.0	2.447638885149441E-4	0.0
10-11	0.0	0.0	0.0	3.671458327724161E-4	0.0
12-13	0.0	0.0	0.0	3.671458327724161E-4	0.0
14-15	0.0	0.0	0.0	3.671458327724161E-4	0.0
16-17	0.0	0.0	0.0	4.895277770298881E-4	0.0
18-19	0.0	0.0	0.0	5.507187491586242E-4	0.0
20-21	0.0	0.0	0.0	0.0010402465261885123	0.0
22-23	0.0	0.0	0.0	0.0020193020802482886	0.0
24-25	0.0	0.0	0.0	0.004895277770298881	0.0
26-27	0.0	0.0	0.0	0.008627927070151777	0.0
28-29	0.0	0.0	0.0	0.01609322566985757	0.0
30-31	0.0	0.0	0.0	0.039101031190262314	0.0
32-33	0.0	0.0	0.0	0.10163820470583051	0.0
34-35	0.0	0.0	0.0	0.1732928330685804	0.0
36-37	0.0	0.0	0.0	0.2906571176114961	0.0
38-39	0.0	0.0	0.0	0.49056802355607665	0.0
40-41	0.0	0.0	0.0	0.7197894051503217	0.0
42-43	0.0	0.0	0.0	0.9316937416321346	0.0
44-45	0.0	0.0	0.0	1.155285553790536	0.0
46-47	0.0	0.0	0.0	1.4105742895116227	0.0
48-49	0.0	0.0	0.0	1.6842815078434588	0.0
50-51	0.0	0.0	0.0	1.968207618520794	0.0
52-53	0.0	0.0	0.0	2.234816684085697	0.0
54-55	0.0	0.0	0.0	2.4989781107654503	0.0
56-57	0.0	0.0	0.0	2.7912261936522933	0.0
58-59	0.0	0.0	0.0	3.1118056966347414	0.0
60-61	0.0	0.0	0.0	3.4466426961231846	0.0
62-63	0.0	0.0	0.0	3.7819080324165295	0.0
64-65	0.0	0.0	0.0	4.10334420900878	0.0
66-67	0.0	0.0	0.0	4.453050614724506	0.0
68-69	0.0	0.0	0.0	4.838370166219157	0.0
70-71	0.0	0.0	0.0	5.243332019767132	0.0
72-73	0.0	0.0	0.0	5.677849112853286	0.0
74-75	0.0	0.0	0.0	6.104288997618447	0.0
76-77	0.0	0.0	0.0	6.534155576822817	0.0
78-79	0.0	0.0	0.0	7.005693208046858	0.0
80-81	0.0	0.0	0.0	7.514067804492397	0.0
82-83	0.0	0.0	0.0	8.059524130047949	0.0
84-85	0.0	0.0	0.0	8.60075827852662	0.0
86-87	0.0	0.0	0.0	9.134527128405583	0.0
88-89	0.0	0.0	0.0	9.727773603193679	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGG	65	2.5940699E-6	43.83234	7
GGTATCA	615	0.0	43.46448	1
GTATCAA	1200	0.0	33.8111	1
ATCAACG	1195	0.0	32.186512	3
TCAACGC	1205	0.0	31.919405	4
TAGTACG	60	0.003956386	31.656693	4
AAATGCG	95	3.4971326E-5	29.990553	6
TCGCACC	340	0.0	28.07842	1
GTACATG	2760	0.0	26.287912	1
CAACGCA	1460	0.0	25.693958	5
CGCAGAG	1510	0.0	25.157639	8
CGCACCA	380	0.0	24.992126	2
ATGCGGG	115	1.2840531E-4	24.774803	8
ACGCAGA	1540	0.0	24.667553	7
AACGCAG	1545	0.0	24.587723	6
TATCAAC	1575	0.0	24.420877	2
ACATGGG	2765	0.0	23.87132	3
ACGGACC	100	0.001656272	23.74252	5
TACATGG	2970	0.0	23.02305	2
AAGACCG	475	0.0	22.992756	5
>>END_MODULE