FastQCFastQC Report
Fri 10 Feb 2017
SRR2568721_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568721_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332408
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27250.11683204653731251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.048.5771371
GTATCAA41400.044.8588831
TCAACGC47900.037.4824834
ATCAACG49750.035.9931873
CAACGCA50200.035.954395
AACGCAG51700.034.9112246
TATCAAC54250.033.6204532
GTACATG73950.029.1601471
ACGCAGA60550.029.1026027
CGCAGAG63200.027.8071638
TACATGG74050.027.7737712
ACATGGG75950.026.7662813
GCAGAGT74000.023.3637059
CATGGGG50750.022.4619394
GGACCGT2701.0040822E-921.1100646
GTATAGG6800.020.2563481
AGAGTAC63900.020.2548210-11
GTATATA15300.019.5578521
CAGAGTA69800.018.9850410-11
GTATAAT12150.018.76451
Produced by FastQC (version 0.11.5)