FastQCFastQC Report
Fri 10 Feb 2017
SRR2568721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332408
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34950.14984513858638798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.051.2261
GTATCAA44650.044.424281
TCAACGC51550.037.697794
ATCAACG52550.036.8900033
CAACGCA53750.036.066415
AACGCAG56000.034.702166
TATCAAC57150.034.035982
CGCAGAG63850.030.0533398
ACGCAGA65650.029.4539577
GTACATG64600.026.1397611
TACATGG65700.024.6118772
GCAGAGT78000.024.6013539
ACATGGG69300.023.1056353
AGAGTAC66950.020.75140610-11
ACCGTGC3900.020.704118
ATTATAC10150.020.1290663
CAGAGTA73500.019.93609210-11
CATGGGG47200.019.5290434
GTATAGC9200.019.1302071
GAGTACT44000.018.78316712-13
Produced by FastQC (version 0.11.5)