FastQCFastQC Report
Fri 10 Feb 2017
SRR2568720_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568720_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137185
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31662.3078324889747424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21071.5358822028647447No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA19391.4134198345300142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17541.2785654408280789No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11310.8244341582534533No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC9280.6764587965156541No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA7630.5561832561869009No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6840.4985967853628312No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA6660.48547581732696726No Hit
GGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT5930.4322630025148522No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.4184130918103291No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG5520.40237635309982867No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5370.3914422130699421No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT5210.37977913037139627No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA5020.3659292196668732No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5010.3652002769982141No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.30396909283084883No Hit
GTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAAT4130.30105332215621244No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.29813755148157595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4050.2952217808069395No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3830.27918504209643913No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA3800.27699821409046177No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA3750.27335350074716624No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3310.2412800233261654No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3180.23180376863359697No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA3100.22597222728432412No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3000.21868280059773298No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA2940.21430914458577832No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.19389874986332326No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2640.19244086452600503No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2630.19171192185734592No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2620.1909829791886868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.18733826584539126No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA2510.1829646098334366No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA2500.1822356671647775No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT2480.18077778182745927No Hit
CCTTAGTACCTTCTTTATTTCTCTTCCATAGTATGCATGATTAGAGTTGG2380.17348835514086816No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.17348835514086816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2350.17130152713489083No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.16984364179757264No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.1683857564602544No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2180.15890950176768598No Hit
GTACATGGGAGCAAATACAAATAAGCAATCCTAAATTCTATCAATCCCCA2180.15890950176768598No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2130.15526478842439043No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2060.15016218974377665No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2030.14797536173779932No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1980.14433064839450377No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG1970.14360170572584466No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1960.14287276305718555No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA1950.14214382038852644No Hit
CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT1930.14068593505120822No Hit
GAGTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTA1900.13849910704523088No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT1870.13631227903925355No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1800.1312096803586398No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1780.1297517950213216No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1770.12902285235266248No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA1730.126107081678026No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1730.126107081678026No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.1246491963407078No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC1710.1246491963407078No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC1690.12319131100338959No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC1650.12027554032875314No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA1610.1173597696541167No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1600.1166308269854576No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1600.1166308269854576No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC1590.11590188431679849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1590.11590188431679849No Hit
CACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCG1530.11152822830484382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1520.11079928563618473No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT1500.10934140029886649No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1480.10788351496154827No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1450.10569668695557094No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC1430.10423880161825273No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA1420.10350985894959361No Hit
GAGTACATGGGAGCAAATACAAATAAGCAATCCTAAATTCTATCAATCCC1400.1020519736122754No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.10132303094361628No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACAG200.00207017271.259
AGGACAC200.00207017271.257
AAGGACA200.00207017271.256
AGTACTC551.1728844E-860.4545445
TAAAGGA250.005011876657.04
TGGTTCT250.005011876657.07
ATGCAAT250.005011876657.03
CAATACC250.005011876657.06
TTTAGTG451.5089152E-552.7777753
GTCATGG451.5089152E-552.7777751
TCATGGG451.5089152E-552.7777752
GACAGCC654.381218E-851.1538479
ATACCAA405.371496E-447.5000046
TACAAGA405.371496E-447.5000044
ATGGGTC502.8138877E-547.55
GGGACAG751.3510726E-744.3333327
GGACAGC751.3510726E-744.3333328
ATGGGAC1400.040.7142875
GGATAGC704.282434E-640.7142878
TGGGAGC1101.5970727E-938.8636366
Produced by FastQC (version 0.11.5)