##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568720_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 137185 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.309684003353137 33.0 31.0 34.0 25.0 34.0 2 30.33201880672085 33.0 31.0 34.0 25.0 34.0 3 30.300820060502243 33.0 31.0 34.0 24.0 34.0 4 33.58670408572366 37.0 35.0 37.0 26.0 37.0 5 33.66124576302074 37.0 35.0 37.0 26.0 37.0 6 33.95045376681124 37.0 35.0 37.0 27.0 37.0 7 33.93812734628421 37.0 35.0 37.0 27.0 37.0 8 33.95301964500492 37.0 35.0 37.0 27.0 37.0 9 35.554156795568026 39.0 37.0 39.0 27.0 39.0 10-11 35.51248678791413 39.0 37.0 39.0 27.0 39.0 12-13 35.44912709115428 39.0 37.0 39.0 27.0 39.0 14-15 36.44655027882057 40.0 37.0 41.0 25.5 41.0 16-17 36.44586142799869 40.0 37.5 41.0 26.5 41.0 18-19 36.62756861172869 40.0 37.5 41.0 26.5 41.0 20-21 36.70456682581915 40.0 37.5 41.0 27.0 41.0 22-23 36.58516966140613 40.0 36.5 41.0 26.5 41.0 24-25 36.469096475562196 40.0 36.0 41.0 26.0 41.0 26-27 36.201166308269855 40.0 35.5 41.0 25.0 41.0 28-29 35.873335277180445 40.0 35.0 41.0 24.0 41.0 30-31 35.407836133688086 39.0 35.0 41.0 22.5 41.0 32-33 34.70972773991326 38.0 35.0 41.0 19.0 41.0 34-35 34.29844006268907 38.0 35.0 41.0 15.5 41.0 36-37 33.888992965703245 38.0 34.5 41.0 13.5 41.0 38-39 33.407777818274596 37.5 33.5 41.0 11.0 41.0 40-41 32.82153661114553 36.5 33.0 40.0 8.5 41.0 42-43 32.14191784816124 35.0 32.0 40.0 8.0 41.0 44-45 31.296187629842912 35.0 28.0 40.0 7.5 41.0 46-47 30.32656996027262 35.0 22.0 40.0 7.0 41.0 48-49 29.44675802748114 35.0 16.5 40.0 7.0 41.0 50-51 28.115027153114408 34.0 12.5 39.0 7.0 40.0 52-53 27.959638444436344 35.0 11.0 39.5 7.0 40.5 54-55 27.969548420016764 35.0 10.0 40.0 7.0 41.0 56-57 27.46643219010825 35.0 10.0 40.0 7.0 41.0 58-59 26.960779239712796 35.0 9.5 40.0 7.0 41.0 60-61 26.466570689215295 35.0 8.0 40.0 7.0 41.0 62-63 25.97533986951926 34.0 8.0 39.5 7.0 41.0 64-65 25.595342056347267 33.0 8.0 39.0 7.0 41.0 66-67 25.243270036811605 33.0 8.0 39.0 7.0 41.0 68-69 24.727422823194956 32.5 7.0 37.5 7.0 41.0 70-71 24.155545431351825 31.0 7.0 37.0 7.0 40.5 72-73 23.656915843568903 29.5 7.0 36.5 7.0 39.5 74-75 23.25726573604986 28.5 7.0 36.0 7.0 39.0 76-77 22.855964573386302 27.5 7.0 35.5 7.0 39.0 78-79 22.500721653241975 26.0 7.0 35.0 7.0 37.5 80-81 22.179549513430768 25.0 7.0 35.0 7.0 37.0 82-83 21.859919087363778 24.5 7.0 35.0 7.0 37.0 84-85 21.567689616211688 24.0 7.0 35.0 7.0 36.0 86-87 21.345048656923133 23.0 7.0 35.0 7.0 36.0 88-89 21.097904289827603 21.0 7.0 35.0 6.5 36.0 90-91 20.833159601997302 20.0 7.0 35.0 5.0 35.5 92-93 20.584185588803443 19.5 7.0 35.0 5.0 35.0 94-95 20.416809417939277 19.0 7.0 35.0 5.0 35.0 96-97 20.223894740678645 19.0 7.0 35.0 5.0 35.0 98-99 20.042646790829902 18.0 7.0 35.0 5.0 35.0 100-101 19.159295841382075 14.0 7.0 34.0 5.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38897041792180787 1101 2 0.3158993741506677 1101 3 0.7554093555179797 1101 4 0.18373400910597582 1101 5 0.2233820969427498 1101 6 0.6794746083503753 1101 7 0.667691224839956 1101 8 0.007158045562107418 1101 9 0.10509622883883196 1101 10-11 0.12689764402040282 1101 12-13 0.6728781068526857 1101 14-15 0.8904737197105064 1101 16-17 0.7217021821441847 1101 18-19 0.6739974379624485 1101 20-21 0.644023262886769 1101 22-23 0.6457536683462237 1101 24-25 0.7178554405645059 1101 26-27 0.8928788734389101 1101 28-29 0.9288889062889325 1101 30-31 1.2259482085529 1101 32-33 1.6286033830527131 1101 34-35 1.3459043925623533 1101 36-37 1.6754522841563642 1101 38-39 1.4069940771891112 1101 40-41 1.8498484146187764 1101 42-43 2.0416248978921914 1101 44-45 2.013397258845469 1101 46-47 1.5258819554719416 1101 48-49 1.3795928521127614 1101 50-51 0.7378432766027423 1101 52-53 1.077336698908372 1101 54-55 0.8799690639613154 1101 56-57 0.5031974805448307 1101 58-59 1.0522889435819067 1101 60-61 0.8061923621808411 1101 62-63 1.0177717803550657 1101 64-65 0.9755963724486811 1101 66-67 0.7857747597408284 1101 68-69 0.32622945765360356 1101 70-71 0.8600925562148873 1101 72-73 1.376651932210148 1101 74-75 0.7257694681443816 1101 76-77 0.9129123917978994 1101 78-79 1.1150650423181787 1101 80-81 0.5573898905455152 1101 82-83 0.5985098234864701 1101 84-85 0.16964784826892298 1101 86-87 0.5314449974215698 1101 88-89 0.5108787583008834 1101 90-91 0.7967783865702955 1101 92-93 0.8263693637301621 1101 94-95 1.1820549425603346 1101 96-97 1.2965425048871175 1101 98-99 0.32908495547169636 1101 100-101 0.1618840128801473 1102 1 0.2742368756020568 1102 2 0.6005388725832681 1102 3 0.8124626470540299 1102 4 0.8075584605166313 1102 5 0.5556704981966689 1102 6 0.43825203781745614 1102 7 0.5567194380060982 1102 8 0.32753422111069597 1102 9 0.4836228746068585 1102 10-11 0.42736786345614064 1102 12-13 0.5772668215862211 1102 14-15 0.8185301460427965 1102 16-17 0.9319686398244329 1102 18-19 0.6925711056740482 1102 20-21 0.7133806296579266 1102 22-23 0.906098495932433 1102 24-25 1.3836077916616816 1102 26-27 0.622424327984362 1102 28-29 0.9654092824644849 1102 30-31 1.4471864530670118 1102 32-33 1.6472554206702696 1102 34-35 1.7484905994589113 1102 36-37 1.529370779454176 1102 38-39 1.680896898505729 1102 40-41 0.9148170666563971 1102 42-43 0.833082578142978 1102 44-45 1.4512499234222638 1102 46-47 1.93701048211771 1102 48-49 2.2734799994481847 1102 50-51 2.3776551888284487 1102 52-53 2.3416000217923845 1102 54-55 2.0106900670961103 1102 56-57 2.053824439792482 1102 58-59 0.9994676269674905 1102 60-61 0.9588569389833488 1102 62-63 0.744652658098012 1102 64-65 0.5708158081853583 1102 66-67 0.7856180199289149 1102 68-69 0.790649520349529 1102 70-71 1.368791615776015 1102 72-73 1.340836885188203 1102 74-75 1.1326660198685197 1102 76-77 1.771924930982852 1102 78-79 1.4974318134780589 1102 80-81 1.4769823670345446 1102 82-83 1.2458452466839596 1102 84-85 1.1620459673911796 1102 86-87 0.623137787390224 1102 88-89 0.5270229589278408 1102 90-91 0.2995997031847182 1102 92-93 1.1077173261126099 1102 94-95 0.8426348798644057 1102 96-97 0.45343905661125916 1102 98-99 0.49350501816762105 1102 100-101 0.07943887181431464 1103 1 0.9146910387643779 1103 2 0.7579540674248726 1103 3 0.9681957561092602 1103 4 1.012632752638865 1103 5 0.7126651715916807 1103 6 0.7963260495920608 1103 7 0.721423671255323 1103 8 1.0181706102332058 1103 9 0.8785625998661786 1103 10-11 0.9766389596153786 1103 12-13 1.0926859412946612 1103 14-15 1.048345116606292 1103 16-17 1.0917317460761922 1103 18-19 0.8278037942078313 1103 20-21 1.3162331668039897 1103 22-23 1.158761798427527 1103 24-25 1.0606122772237825 1103 26-27 1.6132779865209486 1103 28-29 1.6573072359267798 1103 30-31 1.6658225618418925 1103 32-33 1.4404289558538963 1103 34-35 1.6719206527249568 1103 36-37 2.028039128206629 1103 38-39 2.310172199879432 1103 40-41 2.1689171507606844 1103 42-43 2.0646847648544977 1103 44-45 2.0964349528587682 1103 46-47 2.0942855031437766 1103 48-49 1.9276711965325681 1103 50-51 2.0628247991452398 1103 52-53 1.6193544372675746 1103 54-55 1.8503312221283537 1103 56-57 1.6913349528286474 1103 58-59 1.329857310174667 1103 60-61 0.8877888145090012 1103 62-63 0.8309646850113879 1103 64-65 0.4303266015691527 1103 66-67 -0.23961326686670859 1103 68-69 0.0631474177422966 1103 70-71 -0.038798796335001384 1103 72-73 -0.16739832647425956 1103 74-75 -0.576263052180817 1103 76-77 -0.02219477745561349 1103 78-79 0.4322860327099036 1103 80-81 -0.027643368877988905 1103 82-83 -0.1647939459148624 1103 84-85 -0.11553323081459155 1103 86-87 0.3183999124252672 1103 88-89 0.08926013302372127 1103 90-91 0.3899417272946124 1103 92-93 0.21901533564442488 1103 94-95 0.2642426735285497 1103 96-97 0.8888189276513465 1103 98-99 1.079011045641689 1103 100-101 0.9500584400789656 1104 1 0.33194562453337895 1104 2 0.47044482869612025 1104 3 0.3529300166750069 1104 4 0.3944778107588647 1104 5 0.7688366423972965 1104 6 0.6108795670280642 1104 7 0.8734763488068964 1104 8 0.6212076323389653 1104 9 0.43980697264048985 1104 10-11 0.44012078451627445 1104 12-13 0.7844483547865693 1104 14-15 0.7020439676443928 1104 16-17 0.6601319342102983 1104 18-19 0.601270165235178 1104 20-21 0.499395163713217 1104 22-23 0.38707598239581387 1104 24-25 0.6331446967628551 1104 26-27 0.8176722618686583 1104 28-29 0.7582277492641438 1104 30-31 0.5941300267347245 1104 32-33 0.3914133004080895 1104 34-35 0.6574746404962397 1104 36-37 0.7072704659745455 1104 38-39 0.8863329201643211 1104 40-41 0.7126583319741471 1104 42-43 0.4457571292971494 1104 44-45 0.25678568859752815 1104 46-47 0.4130720381165709 1104 48-49 0.9609978107904489 1104 50-51 1.4642895575944763 1104 52-53 1.8207251286604382 1104 54-55 1.3369938573497464 1104 56-57 0.3284040921150826 1104 58-59 -0.006801965508998364 1104 60-61 -0.09215474525717582 1104 62-63 -0.05041003782675091 1104 64-65 0.25038976087843423 1104 66-67 0.9432127762697569 1104 68-69 0.7630063171577355 1104 70-71 0.7906710686115836 1104 72-73 0.664255237995274 1104 74-75 1.0352736003757919 1104 76-77 0.264978507500377 1104 78-79 0.8910981001694225 1104 80-81 0.5421006343471646 1104 82-83 0.17082387307324254 1104 84-85 0.5460941292606591 1104 86-87 0.6740069808926457 1104 88-89 0.30302751863146327 1104 90-91 0.043472601446328696 1104 92-93 0.38628671910206336 1104 94-95 0.48288138884132437 1104 96-97 0.5771210172838153 1104 98-99 0.27991140175268825 1104 100-101 0.4329583930454355 1105 1 0.4091063567493336 1105 2 0.4279639450937438 1105 3 0.6356982255264754 1105 4 0.784838512079638 1105 5 0.13672109439389857 1105 6 -0.002853344071034769 1105 7 0.1530778008807374 1105 8 0.36153357657315155 1105 9 0.10552103767485477 1105 10-11 0.4920633194664532 1105 12-13 0.6312468409223513 1105 14-15 0.5416207035677658 1105 16-17 0.8927727004109656 1105 18-19 0.9700042349038256 1105 20-21 0.5433435687491297 1105 22-23 0.6494919861032358 1105 24-25 1.128008371745473 1105 26-27 1.0932187205077213 1105 28-29 1.3319900107919054 1105 30-31 0.9892022442368429 1105 32-33 0.4222312505123611 1105 34-35 0.9711380374221719 1105 36-37 0.4579076792285832 1105 38-39 0.471140211428704 1105 40-41 0.8730429770656798 1105 42-43 0.17569456654129567 1105 44-45 -0.07908362475691533 1105 46-47 0.333401071869563 1105 48-49 0.72916804327674 1105 50-51 0.27420691296637756 1105 52-53 -0.0074976256455769885 1105 54-55 0.29976515572002427 1105 56-57 0.27715669889657235 1105 58-59 0.2187715264196335 1105 60-61 0.9524115635402275 1105 62-63 0.7919858840084224 1105 64-65 0.797813874572725 1105 66-67 0.8068877588912109 1105 68-69 1.4039269937623544 1105 70-71 1.2362182996303481 1105 72-73 1.2535423315304541 1105 74-75 1.1524049821630769 1105 76-77 0.5757840995294181 1105 78-79 0.8400438018763836 1105 80-81 1.1780780808598728 1105 82-83 1.2546270707252098 1105 84-85 1.0990021388381663 1105 86-87 1.38127507388716 1105 88-89 0.8549314345965477 1105 90-91 1.4611369252278976 1105 92-93 2.0920872906630414 1105 94-95 2.0272546027132634 1105 96-97 1.7558882992796434 1105 98-99 1.5273432392439972 1105 100-101 1.500966425794335 1106 1 0.12748151312581513 1106 2 0.5420267900418629 1106 3 0.9201194485745319 1106 4 0.11966815370153938 1106 5 0.27492110911168766 1106 6 0.3701689533754262 1106 7 -0.1352436355752218 1106 8 0.0344307728348312 1106 9 0.6516245037135633 1106 10-11 0.35724839563099664 1106 12-13 0.40050874393213576 1106 14-15 0.44444651856519357 1106 16-17 -0.01892058091952009 1106 18-19 -0.14998251908837545 1106 20-21 0.42784573962263295 1106 22-23 0.538667054172997 1106 24-25 0.8121646746375148 1106 26-27 0.7749833831024731 1106 28-29 0.5831122420083332 1106 30-31 0.46385085708546825 1106 32-33 0.714931228435681 1106 34-35 0.5459043925623561 1106 36-37 0.5172203586016124 1106 38-39 0.8815824809113764 1106 40-41 0.8454103330153373 1106 42-43 0.9212240389086617 1106 44-45 1.7003335508998667 1106 46-47 1.5498261215420932 1106 48-49 1.5688913488915723 1106 50-51 1.17184470824197 1106 52-53 1.2553252457945554 1106 54-55 1.1670843109190798 1106 56-57 2.0561323409600085 1106 58-59 1.9359324654144103 1106 60-61 2.0306733929610843 1106 62-63 2.2309070702620097 1106 64-65 1.698395227137297 1106 66-67 2.510434745424437 1106 68-69 2.0917627432656296 1106 70-71 1.994809807467572 1106 72-73 1.716379920757035 1106 74-75 1.6206513579081623 1106 76-77 1.6313805378250983 1106 78-79 1.4399039829051539 1106 80-81 1.8394586092284086 1106 82-83 1.7035563517613461 1106 84-85 1.3087673901443715 1106 86-87 1.5817313252669543 1106 88-89 1.5126325163538503 1106 90-91 1.6406939198559058 1106 92-93 1.5223249829141317 1106 94-95 1.3397500034049976 1106 96-97 1.2898495915012944 1106 98-99 1.991468634784507 1106 100-101 1.6794931753711957 1107 1 -0.029211400260010123 1107 2 -0.193890835639543 1107 3 0.22254222265084778 1107 4 0.0571605825325463 1107 5 -0.13955496599430717 1107 6 0.22353055240631647 1107 7 0.36839052553925455 1107 8 0.05750769591175242 1107 9 -0.4767219529793465 1107 10-11 -0.1640114468886864 1107 12-13 0.25259838657296285 1107 14-15 0.011103090339879884 1107 16-17 -0.21571040526839624 1107 18-19 -0.0015270375620275445 1107 20-21 -0.20737533851183088 1107 22-23 0.2803690529616105 1107 24-25 0.022400895109953467 1107 26-27 0.2858858664459021 1107 28-29 -0.14453766713764082 1107 30-31 -0.04293291032821145 1107 32-33 -0.7402777358283998 1107 34-35 -0.9079417612838014 1107 36-37 -0.6346875759834916 1107 38-39 -0.7360129158178808 1107 40-41 -0.5889627741924102 1107 42-43 -0.9440394377721439 1107 44-45 -0.9470922516440474 1107 46-47 -1.3461460165560304 1107 48-49 -1.0770505045305967 1107 50-51 -1.0642546254951597 1107 52-53 -1.4135723920007202 1107 54-55 -1.4812197472274953 1107 56-57 -1.1167325893852365 1107 58-59 -1.2022565109635437 1107 60-61 -0.3487027427142628 1107 62-63 -0.5343261217428363 1107 64-65 -0.3373406904883822 1107 66-67 -0.30058887662280753 1107 68-69 -0.17446984304569924 1107 70-71 -0.3448025486802173 1107 72-73 -0.6516697461115335 1107 74-75 -0.858845916471001 1107 76-77 -0.8961785172930128 1107 78-79 -1.025844048590912 1107 80-81 -0.9817709486153241 1107 82-83 -0.8581335331568916 1107 84-85 -0.39783466921359434 1107 86-87 -0.5958277298511554 1107 88-89 -0.7321282347061135 1107 90-91 -0.6679069281150163 1107 92-93 -0.7162879789271805 1107 94-95 -0.4364765259711376 1107 96-97 0.11961942796404301 1107 98-99 -0.23699896061222248 1107 100-101 -0.23846563746950622 1108 1 0.7826767116281026 1108 2 0.7082070664583604 1108 3 0.9225422226508471 1108 4 1.082335407707376 1108 5 0.60100447456513 1108 6 0.8060480349238048 1108 7 0.399859057007788 1108 8 0.6428223812264378 1108 9 0.605096228838832 1108 10-11 0.9220025391253017 1108 12-13 0.3581927921673653 1108 14-15 0.3002639295007157 1108 16-17 0.5584154688574756 1108 18-19 0.6082631722281846 1108 20-21 0.7838834027469161 1108 22-23 0.7513480739406262 1108 24-25 0.8559673286763925 1108 26-27 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0.5852116976553745 2316 52-53 1.0129826319227249 2316 54-55 1.136906863004377 2316 56-57 1.1833410212146909 2316 58-59 1.8233415751608568 2316 60-61 1.2975799219894526 2316 62-63 1.432843550689995 2316 64-65 1.192103549482173 2316 66-67 1.0439087310326975 2316 68-69 1.0924974002373347 2316 70-71 1.1538724605211073 2316 72-73 0.915886381970914 2316 74-75 1.1685924346515613 2316 76-77 1.2712855975395208 2316 78-79 1.3141081045191392 2316 80-81 1.2073898905455138 2316 82-83 0.9131031249218751 2316 84-85 1.2220401927665314 2316 86-87 0.9202009782828142 2316 88-89 0.8345629688271963 2316 90-91 1.0223764726946953 2316 92-93 1.4089052488976286 2316 94-95 1.5677008755746868 2316 96-97 1.7608965718727667 2316 98-99 1.7331519411176295 2316 100-101 1.5599701372820611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 18.0 7 597.0 8 1393.0 9 1242.0 10 1291.0 11 1450.0 12 1585.0 13 1479.0 14 1233.0 15 1428.0 16 2274.0 17 3616.0 18 3725.0 19 4734.0 20 6339.0 21 7724.0 22 5464.0 23 4950.0 24 4692.0 25 3450.0 26 3057.0 27 2749.0 28 2696.0 29 2071.0 30 1877.0 31 1943.0 32 2112.0 33 2636.0 34 3493.0 35 5411.0 36 8149.0 37 14317.0 38 20492.0 39 7497.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.621181084568335 19.713658796171426 12.914513154345784 13.750646964914454 2 15.118423642447349 25.408778439534323 41.290449565160344 18.182348352857986 3 21.042388016182528 38.43933374640084 28.603710318183474 11.914567919233154 4 10.554437835316072 19.908298343830182 37.42273151387917 32.114532306974574 5 8.415343568210645 46.42799865867705 30.011372085902988 15.14528568720932 6 22.23178030662011 38.049761979398276 20.3954130914976 19.323044622484016 7 21.450025515783334 40.84785302908799 22.52824961726325 15.173871837865422 8 28.92031785375811 30.906903841947948 20.287963840489905 19.884814463804037 9 33.35982211205483 14.450479349688331 21.132942077060473 31.056756461196372 10-11 23.272700761856157 32.630773156417455 22.971239018700107 21.12528706302628 12-13 28.6961402894874 31.482028234316395 24.785764014186412 15.036067462009791 14-15 26.792652257899917 28.61355104421037 29.489375660604296 15.104421037285418 16-17 17.341546087400225 33.196413602070194 30.935597915223966 18.526442395305608 18-19 18.232678499835988 28.746218609906332 38.826037832124506 14.195065058133178 20-21 19.245530588814173 28.991671678238845 37.798188544457204 13.964609188489785 22-23 17.719567443843875 29.84812424144127 35.530253053369734 16.902055261345122 24-25 17.06103614932097 31.26161786253198 34.35679868203322 17.32054730611382 26-27 17.475915713197423 29.2011270316276 34.660596539444285 18.662360715730696 28-29 16.78306384591832 31.68967880210535 36.98732996048814 14.539927391488183 30-31 17.367323578089344 33.7879218705571 35.62705512660674 13.217699424746824 32-33 14.000225974698125 34.220587752900315 37.05912154158481 14.720064730816754 34-35 12.487152385464883 34.90906440208478 36.1672194481904 16.43656376425994 36-37 13.900572219994897 35.29686190181142 34.8514779312607 15.951087946932974 38-39 15.349037245461405 33.220589789662824 36.16151970521451 15.268853259661258 40-41 13.83350949447826 35.805299413201155 34.94150235084011 15.419688741480483 42-43 14.930987101312464 35.94757425219321 33.25047654800652 15.870962098487803 44-45 15.884066000153082 34.41316795632064 33.01319784373257 16.689568199793705 46-47 14.237913337610077 34.723639703738264 33.66295853502071 17.375488423630955 48-49 14.68859008051788 34.57931321054569 33.09871732720003 17.633379381736404 50-51 14.679735083888886 34.001698554042086 32.5597501011478 18.75881626092123 52-53 13.902030105332216 34.25374494296023 31.042752487516857 20.80147246419069 54-55 15.066880489849474 33.31231548638699 30.742063636694972 20.878740387068557 56-57 15.437547836862631 32.042497357582825 29.87935998833692 22.640594817217625 58-59 15.244378029667965 30.698691547909757 31.226810511353282 22.830119911068994 60-61 14.83070306520392 30.680832452527607 31.140066333782844 23.34839814848562 62-63 14.732671679490311 28.387938606882123 32.75915484230745 24.12023487132011 64-65 15.239799674872609 27.946375850178967 33.12946047806848 23.68436399687994 66-67 16.45696040123637 25.67650317839855 33.62286405785269 24.24367236251239 68-69 16.828456550029706 26.47752499753943 33.191410251269474 23.502608201161387 70-71 15.307487361910422 25.719742390721983 35.379725843663095 23.5930444037045 72-73 16.29842912854904 24.87152385464883 34.370740241280025 24.459306775522105 74-75 15.474359441629915 24.905055217407153 34.883186937347375 24.737398403615558 76-77 15.450304333564166 24.54787330976419 35.690490942887344 24.311331413784306 78-79 15.879651565404382 25.300142143820388 35.46123847359405 23.358967817181178 80-81 16.18994845172909 24.60281583341232 35.20010499223495 24.00713072262364 82-83 16.180126714251344 24.476702221509345 34.8978193191843 24.445351745055007 84-85 15.313190378628248 25.929436468761164 33.87530713874287 24.882066013867725 86-87 16.560460792533995 26.141227078852392 31.732711166198825 25.565600962414788 88-89 17.281781329289274 25.374391204176266 30.718306162326108 26.62552130420835 90-91 17.848393823182025 24.494378745680166 30.33435891453652 27.32286851660129 92-93 19.363904786206394 23.667480771333796 29.876426202019466 27.092188240440347 94-95 19.02999766668611 22.776789360088667 30.255789535087207 27.937423438138016 96-97 20.22965460676205 22.793401986694615 28.79978128132689 28.177162125216444 98-99 21.111495907988992 23.170078194776078 27.416656034121356 28.301769863113574 100-101 21.10006124413077 21.20213479541544 27.346233485957594 30.351570474496192 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 223.0 1 137.0 2 144.5 3 258.5 4 178.0 5 107.0 6 183.5 7 296.5 8 394.0 9 365.0 10 256.5 11 200.5 12 191.0 13 194.5 14 219.5 15 240.0 16 259.5 17 284.5 18 306.0 19 348.0 20 415.0 21 539.5 22 692.5 23 860.5 24 1134.5 25 1459.5 26 1818.5 27 2295.0 28 2898.0 29 3461.5 30 3961.0 31 5118.5 32 6812.0 33 7621.0 34 7133.5 35 6037.5 36 6324.5 37 7204.0 38 6213.5 39 4755.0 40 3891.0 41 3139.0 42 2739.5 43 2848.0 44 2924.5 45 2748.0 46 2438.0 47 2597.0 48 3268.0 49 3703.0 50 4687.5 51 4467.0 52 3020.0 53 2271.0 54 1614.5 55 1247.0 56 987.0 57 830.0 58 801.5 59 831.0 60 872.0 61 759.5 62 544.0 63 371.5 64 292.0 65 252.5 66 225.0 67 205.0 68 194.5 69 156.5 70 126.0 71 109.0 72 91.0 73 83.5 74 82.0 75 62.0 76 40.0 77 37.5 78 30.0 79 28.5 80 30.5 81 26.0 82 20.0 83 14.0 84 10.0 85 12.0 86 8.0 87 3.5 88 5.0 89 5.0 90 3.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0043736560119546596 2 0.00583154134927288 3 0.0 4 7.2894266865911E-4 5 0.00510259868061377 6 0.008747312023909319 7 0.010934140029886649 8 0.010934140029886649 9 0.0145788533731822 10-11 0.0145788533731822 12-13 0.008382840689579766 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.001822356671647775 22-23 3.64471334329555E-4 24-25 0.00291577067463644 26-27 0.007653898020920654 28-29 0.008018369355250209 30-31 0.02041039472245508 32-33 0.0010934140029886649 34-35 0.0 36-37 0.0 38-39 3.64471334329555E-4 40-41 0.0 42-43 3.64471334329555E-4 44-45 0.002551299340306885 46-47 0.006560484017931989 48-49 0.007653898020920654 50-51 0.0069249553522615455 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.002551299340306885 64-65 0.00583154134927288 66-67 0.006560484017931989 68-69 0.015672267376170865 70-71 3.64471334329555E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.02332616539709152 82-83 0.01895250938513686 84-85 0.02332616539709152 86-87 0.021868280059773298 88-89 0.02113933739111419 90-91 0.019681452053795966 92-93 0.0145788533731822 94-95 0.029886649415023506 96-97 0.016401210044829976 98-99 0.020045923388125525 100-101 0.02113933739111419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 137185.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.90283194226774 #Duplication Level Percentage of deduplicated Percentage of total 1 84.9260534261592 32.18937930531764 2 6.146508452410716 4.659401538069031 3 2.396292093774641 2.724787695447753 4 1.2923822528222781 1.9593978933556875 5 0.8558186049195146 1.6218974377665198 6 0.6173433082677847 1.4039435798374458 7 0.4134853933880801 1.0970587163319605 8 0.33463469046291133 1.0146881947734812 9 0.2653999269188607 0.9053467944746145 >10 2.1539704213704636 16.68258191493239 >50 0.37117526011116025 9.903415096402668 >100 0.1961651633748101 13.50584976491599 >500 0.02115506663845991 4.97211794292379 >1k 0.009615939381118141 7.360134125451033 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3166 2.3078324889747424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2107 1.5358822028647447 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1939 1.4134198345300142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1754 1.2785654408280789 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1131 0.8244341582534533 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 928 0.6764587965156541 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 763 0.5561832561869009 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 684 0.4985967853628312 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 666 0.48547581732696726 No Hit GGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 593 0.4322630025148522 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 574 0.4184130918103291 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 552 0.40237635309982867 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 537 0.3914422130699421 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 521 0.37977913037139627 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 502 0.3659292196668732 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 501 0.3652002769982141 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 417 0.30396909283084883 No Hit GTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAAT 413 0.30105332215621244 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 409 0.29813755148157595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 405 0.2952217808069395 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 383 0.27918504209643913 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 380 0.27699821409046177 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 375 0.27335350074716624 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 331 0.2412800233261654 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 318 0.23180376863359697 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 310 0.22597222728432412 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 300 0.21868280059773298 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 294 0.21430914458577832 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 266 0.19389874986332326 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 264 0.19244086452600503 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 263 0.19171192185734592 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 262 0.1909829791886868 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 257 0.18733826584539126 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 251 0.1829646098334366 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 250 0.1822356671647775 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 248 0.18077778182745927 No Hit CCTTAGTACCTTCTTTATTTCTCTTCCATAGTATGCATGATTAGAGTTGG 238 0.17348835514086816 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 0.17348835514086816 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 235 0.17130152713489083 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 233 0.16984364179757264 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 231 0.1683857564602544 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 218 0.15890950176768598 No Hit GTACATGGGAGCAAATACAAATAAGCAATCCTAAATTCTATCAATCCCCA 218 0.15890950176768598 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 213 0.15526478842439043 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 206 0.15016218974377665 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 203 0.14797536173779932 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 198 0.14433064839450377 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 197 0.14360170572584466 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 196 0.14287276305718555 No Hit GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA 195 0.14214382038852644 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 193 0.14068593505120822 No Hit GAGTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTA 190 0.13849910704523088 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 187 0.13631227903925355 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 180 0.1312096803586398 No Hit CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.1297517950213216 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 177 0.12902285235266248 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 173 0.126107081678026 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 173 0.126107081678026 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 171 0.1246491963407078 No Hit GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC 171 0.1246491963407078 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 169 0.12319131100338959 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 165 0.12027554032875314 No Hit GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA 161 0.1173597696541167 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 160 0.1166308269854576 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 160 0.1166308269854576 No Hit ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC 159 0.11590188431679849 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 159 0.11590188431679849 No Hit CACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCG 153 0.11152822830484382 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 152 0.11079928563618473 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 150 0.10934140029886649 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 148 0.10788351496154827 No Hit ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 145 0.10569668695557094 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 143 0.10423880161825273 No Hit CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA 142 0.10350985894959361 No Hit GAGTACATGGGAGCAAATACAAATAAGCAATCCTAAATTCTATCAATCCC 140 0.1020519736122754 No Hit ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.10132303094361628 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.2894266865911E-4 0.0 5 0.0 0.0 0.0 7.2894266865911E-4 0.0 6 0.0 0.0 0.0 7.2894266865911E-4 0.0 7 0.0 0.0 0.0 7.2894266865911E-4 0.0 8 0.0 0.0 0.0 7.2894266865911E-4 0.0 9 0.0 0.0 0.0 7.2894266865911E-4 0.0 10-11 0.0 0.0 0.0 0.00291577067463644 0.0 12-13 0.0 0.0 0.0 0.00364471334329555 0.0 14-15 0.0 0.0 0.0 0.00583154134927288 0.0 16-17 0.0 0.0 0.0 0.01166308269854576 0.0 18-19 0.0 0.0 0.0 0.01895250938513686 0.0 20-21 0.0 0.0 0.0 0.030251120749353065 0.0 22-23 0.0 0.0 0.0 0.060137770164376575 0.0 24-25 0.0 0.0 0.0 0.12428472500637824 0.0 26-27 0.0 0.0 0.0 0.17786201115282285 0.0 28-29 0.0 0.0 0.0 0.285016583445712 0.0 30-31 0.0 0.0 0.0 0.4461129132193753 0.0 32-33 0.0 0.0 0.0 0.6611510004738127 0.0 34-35 0.0 0.0 0.0 0.9968290993913329 0.0 36-37 0.0 0.0 0.0 1.4032146371687868 0.0 38-39 0.0 0.0 0.0 1.905820607209243 0.0 40-41 0.0 0.0 0.0 2.615081823814557 0.0 42-43 0.0 0.0 0.0 3.081969603090717 0.0 44-45 0.0 0.0 0.0 3.419470058679885 0.0 46-47 0.0 0.0 0.0 3.778838794328826 0.0 48-49 0.0 0.0 0.0 4.124357619273244 0.0 50-51 0.0 0.0 0.0 4.460400189525094 0.0 52-53 0.0 0.0 0.0 4.8146663264934215 0.0 54-55 0.0 0.0 0.0 5.138316871378066 0.0 56-57 0.0 0.0 0.0 5.4138572001312095 0.0 58-59 0.0 0.0 0.0 5.6456609687648065 0.0 60-61 0.0 0.0 0.0 5.886576520756643 0.0 62-63 0.0 0.0 0.0 6.116193461384262 0.0 64-65 0.0 0.0 0.0 6.36366949739403 0.0 66-67 0.0 0.0 0.0 6.586361482669388 0.0 68-69 0.0 0.0 0.0 6.882312206144986 0.0 70-71 0.0 0.0 0.0 7.235120457775996 0.0 72-73 0.0 0.0 0.0 7.528884353245617 0.0 74-75 0.0 0.0 0.0 7.814265408025658 0.0 76-77 0.0 0.0 0.0 8.069759813390677 0.0 78-79 0.0 0.0 0.0 8.416372052338083 0.0 80-81 0.0 0.0 0.0 8.749134380580967 0.0 82-83 0.0 0.0 0.0 9.13984765098225 0.0 84-85 0.0 0.0 0.0 9.482450705252031 0.0 86-87 0.0 0.0 0.0 9.818857746838212 0.0 88-89 0.0 0.0 0.0 10.173488355140869 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACACAG 20 0.002070172 71.25 9 AGGACAC 20 0.002070172 71.25 7 AAGGACA 20 0.002070172 71.25 6 AGTACTC 55 1.1728844E-8 60.454544 5 TAAAGGA 25 0.0050118766 57.0 4 TGGTTCT 25 0.0050118766 57.0 7 ATGCAAT 25 0.0050118766 57.0 3 CAATACC 25 0.0050118766 57.0 6 TTTAGTG 45 1.5089152E-5 52.777775 3 GTCATGG 45 1.5089152E-5 52.777775 1 TCATGGG 45 1.5089152E-5 52.777775 2 GACAGCC 65 4.381218E-8 51.153847 9 ATACCAA 40 5.371496E-4 47.500004 6 TACAAGA 40 5.371496E-4 47.500004 4 ATGGGTC 50 2.8138877E-5 47.5 5 GGGACAG 75 1.3510726E-7 44.333332 7 GGACAGC 75 1.3510726E-7 44.333332 8 ATGGGAC 140 0.0 40.714287 5 GGATAGC 70 4.282434E-6 40.714287 8 TGGGAGC 110 1.5970727E-9 38.863636 6 >>END_MODULE