FastQCFastQC Report
Fri 10 Feb 2017
SRR2568720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137185
Sequences flagged as poor quality0
Sequence length101
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT60154.384590151984547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33902.4711156467543827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24171.7618544301490686No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT22701.6546998578561796RNA PCR Primer, Index 26 (100% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18711.3638517330611948No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA16771.2224368553413274No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT14231.0372854175019135RNA PCR Primer, Index 26 (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11450.8346393556146809No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC10150.7398768086889965No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA7230.5270255494405365No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.5219229507599227No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6910.5036993840434449No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG6360.46360753726719395RNA PCR Primer, Index 26 (100% over 29bp)
GGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT6250.4555891679119437No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA5840.42570251849692026No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT5760.4198709771476473RNA PCR Primer, Index 26 (100% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5670.41331049312971535No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.3943579837445785No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG5260.38342384371469185No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5150.3754054743594416No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT4900.35718190764296387No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA4630.3375004555891679No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.31636111819805374No Hit
GTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAAT3940.2872034114516893No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3430.2500273353500747No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA3390.2471115646754383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.2471115646754383No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA3290.23982213798884716No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3270.23836425265152897No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3180.23180376863359697No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA3170.23107482596493784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.22815905529030142No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3070.22378539927834676No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA2990.21795385792907385No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG2870.20920654590516455RNA PCR Primer, Index 26 (96% over 27bp)
CCTTAGTACCTTCTTTATTTCTCTTCCATAGTATGCATGATTAGAGTTGG2660.19389874986332326No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2650.19316980719466414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2650.19316980719466414No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC2630.19171192185734592No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA2550.18588038050807304No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2460.17931989649014105No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2450.17859095382148193No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.17348835514086816No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2380.17348835514086816No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA2360.17203046980354997No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT2260.16474104311695886No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC2250.16401210044829972RNA PCR Primer, Index 26 (95% over 22bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2220.1618252724423224No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2160.15745161643036773No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT2090.15234901774975398No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.15162007508109487No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.14870430440645843No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2020.1472464190691402No Hit
GTACATGGGAGCAAATACAAATAAGCAATCCTAAATTCTATCAATCCCCA2010.1465174764004811No Hit
GCTTTATACCAAGTCTTGATATCTAAAAATTAAGTTATCCTACTTTTTTT1980.14433064839450377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1940.14141487771986733No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC1930.14068593505120822No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC1870.13631227903925355No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1850.13485439370193533No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA1840.13412545103327625No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG1830.13339650836461714No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1830.13339650836461714No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT1820.132667565695958No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1780.1297517950213216No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC1780.1297517950213216RNA PCR Primer, Index 26 (96% over 28bp)
CGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAA1780.1297517950213216No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.12756496701534425No Hit
GAGTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTA1740.12683602434668514No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT1720.12537813900936692RNA PCR Primer, Index 26 (100% over 21bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1660.12100448299741226No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1650.12027554032875314No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC1640.11954659766009403No Hit
CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT1620.11808871232277582No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.1166308269854576No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1570.11444399897948027No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1520.11079928563618473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1520.11079928563618473No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA1520.11079928563618473RNA PCR Primer, Index 26 (100% over 29bp)
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA1470.10715457229288916No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA1460.10642562962423006No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1450.10569668695557094No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.10423880161825273No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA1400.1020519736122754No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1400.1020519736122754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT201.7265655E-595.1299741
CTTAGTA254.5714478E-795.095252
CTATGTC200.00206494371.295424
TGGGAAC200.00206494371.295426
TCCTATG301.29074E-463.3968352
TAAACGC452.0253174E-763.3737074
TACAATG352.7655403E-454.3401452
TTAGTAC451.5032278E-552.8114243
TAGTACC451.5032278E-552.8114244
TATATGG405.345003E-447.5476262
TAATACC405.3546345E-447.530284
TATGGGA405.3546345E-447.530284
ATATGGG405.3546345E-447.530283
ATACCAA405.3546345E-447.530286
TCTGAGT205.3834094E-447.49562540-41
GTGAGTT205.3834094E-447.49562570-71
GGTCTGC205.3834094E-447.49562560-61
AGGCTTG205.3834094E-447.49562550-51
GTATAAA554.900645E-543.24091
GGGAAGG554.921819E-543.2093477
Produced by FastQC (version 0.11.5)