##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568720_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 137185 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.313365163829864 33.0 31.0 34.0 30.0 34.0 2 32.332601960855776 33.0 31.0 34.0 30.0 34.0 3 32.326260159638444 34.0 31.0 34.0 30.0 34.0 4 35.89888836243029 37.0 35.0 37.0 35.0 37.0 5 36.02735721835478 37.0 35.0 37.0 35.0 37.0 6 36.12677770893319 37.0 36.0 37.0 35.0 37.0 7 36.02983562342822 37.0 35.0 37.0 35.0 37.0 8 36.06421255968218 37.0 36.0 37.0 35.0 37.0 9 37.79265225789992 39.0 38.0 39.0 35.0 39.0 10-11 37.71916390275905 39.0 38.0 39.0 35.0 39.0 12-13 37.66506177789117 39.0 37.5 39.0 35.0 39.0 14-15 38.73683347304735 40.0 38.0 41.0 34.5 41.0 16-17 38.70072165324197 40.0 38.0 41.0 34.5 41.0 18-19 38.991748368990784 40.5 38.5 41.0 35.5 41.0 20-21 39.06288588402522 41.0 39.0 41.0 35.5 41.0 22-23 38.93848088347852 40.0 38.5 41.0 35.0 41.0 24-25 38.811123665123745 40.0 38.0 41.0 35.0 41.0 26-27 38.51273098370813 40.0 37.5 41.0 35.0 41.0 28-29 38.24833254364544 40.0 37.0 41.0 34.0 41.0 30-31 37.801654699857856 40.0 35.5 41.0 34.0 41.0 32-33 37.02121587637133 39.0 35.0 41.0 33.0 41.0 34-35 36.542140175675186 39.0 35.0 41.0 31.5 41.0 36-37 36.14380216495972 39.0 35.0 41.0 30.5 41.0 38-39 35.40486933702664 38.0 35.0 41.0 26.0 41.0 40-41 34.98600430076175 38.0 35.0 41.0 23.5 41.0 42-43 34.32220723840069 38.0 35.0 41.0 17.5 41.0 44-45 33.54070051390458 37.5 33.5 41.0 11.0 41.0 46-47 32.42940190254036 37.0 32.0 40.0 7.5 41.0 48-49 31.463753325800926 36.5 29.5 40.0 7.0 41.0 50-51 30.632627473849183 36.0 24.0 40.0 7.0 41.0 52-53 30.49203994605824 36.0 22.0 40.0 7.0 41.0 54-55 30.008987863104565 36.0 19.0 40.0 7.0 41.0 56-57 29.409993803987316 35.0 16.0 40.0 7.0 41.0 58-59 28.929412836680395 35.0 13.5 40.0 7.0 41.0 60-61 28.185162371979445 35.0 10.0 40.0 7.0 41.0 62-63 27.523606808324523 35.0 10.0 39.5 7.0 41.0 64-65 26.90121733425666 35.0 10.0 39.0 7.0 41.0 66-67 26.079830156358202 33.5 9.0 39.0 7.0 41.0 68-69 25.05573860115902 32.5 8.0 37.5 7.0 41.0 70-71 24.314695484200165 31.0 7.0 37.0 7.0 40.0 72-73 23.812854903961806 30.0 7.0 36.5 7.0 39.5 74-75 23.46716477749025 29.0 7.0 36.0 7.0 39.0 76-77 22.23844079163174 26.5 7.0 34.5 7.0 37.5 78-79 22.848543937019354 29.0 7.0 35.0 7.0 37.0 80-81 22.80888216641761 29.0 7.0 35.0 7.0 37.0 82-83 22.676513467215806 28.5 7.0 35.0 7.0 37.0 84-85 22.465699602726247 27.0 7.0 35.0 7.0 36.0 86-87 22.307941830375043 27.0 7.0 35.0 7.0 36.0 88-89 22.12921602215986 26.5 7.0 35.0 7.0 36.0 90-91 21.976436928235593 26.0 7.0 35.0 7.0 35.5 92-93 21.867164777490252 25.0 7.0 35.0 7.0 35.0 94-95 21.85778693005795 25.0 7.0 35.0 7.0 35.0 96-97 21.79034515435361 25.0 7.0 35.0 6.0 35.0 98-99 21.77647702008237 25.0 7.0 35.0 5.0 35.0 100-101 21.165054488464484 22.5 7.0 34.5 5.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.516887049035752 1101 2 0.38794562220468976 1101 3 0.5843213102890488 1101 4 0.2345485441100692 1101 5 0.4569432283710242 1101 6 0.5153681234109371 1101 7 0.3587584294999999 1101 8 0.5214054338922622 1101 9 0.7712235840735104 1101 10-11 0.3354766406730363 1101 12-13 0.3139614296745634 1101 14-15 0.4533597987269786 1101 16-17 0.4938510474385325 1101 18-19 0.4328453113854138 1101 20-21 0.4118411382452152 1101 22-23 0.4330770589368882 1101 24-25 0.709413302505915 1101 26-27 0.8749866099901382 1101 28-29 0.4318076762978862 1101 30-31 0.1375375548150899 1101 32-33 0.8894046987797068 1101 34-35 0.9086268764565233 1101 36-37 1.2746268865414763 1101 38-39 1.0755348061407801 1101 40-41 1.1289763811170062 1101 42-43 1.2267021496730948 1101 44-45 1.5502342447161297 1101 46-47 1.2070251691706773 1101 48-49 1.2395490814768948 1101 50-51 1.4998675242286765 1101 52-53 1.360830424597701 1101 54-55 1.0396392046880862 1101 56-57 1.0148987616713292 1101 58-59 0.3481784065117175 1101 60-61 0.3203079234999322 1101 62-63 0.5308900959792098 1101 64-65 1.3251213930302619 1101 66-67 1.75385493123175 1101 68-69 1.4266945100725579 1101 70-71 1.3319021594236027 1101 72-73 1.341000796807542 1101 74-75 1.3817839460630772 1101 76-77 1.3362856998296273 1101 78-79 1.1465934067670212 1101 80-81 1.474946950705693 1101 82-83 1.5623328487771708 1101 84-85 1.33291808571229 1101 86-87 1.222634164060885 1101 88-89 1.4880504638645 1101 90-91 2.1914640635521714 1101 92-93 2.588576765774363 1101 94-95 1.8950764588980356 1101 96-97 1.9148577414112253 1101 98-99 1.885505403358021 1101 100-101 0.9252919536177195 1102 1 0.5910249800702374 1102 2 0.33935628051190037 1102 3 0.31990124759312266 1102 4 0.2021034030442408 1102 5 -0.02628561175436772 1102 6 0.25596373469620204 1102 7 0.3706706552053305 1102 8 0.15228317683897075 1102 9 0.2828223301550139 1102 10-11 0.38226347452884113 1102 12-13 0.3415476365711214 1102 14-15 0.1988143441815282 1102 16-17 -0.08083547293764326 1102 18-19 0.3942873176550066 1102 20-21 0.3881734266464676 1102 22-23 0.05893912790240563 1102 24-25 0.3476578166124966 1102 26-27 0.33517469776443676 1102 28-29 0.4325130054514901 1102 30-31 0.07194194352982919 1102 32-33 0.1523357332624684 1102 34-35 0.04467703319632932 1102 36-37 0.09147641632203118 1102 38-39 0.2901899785545794 1102 40-41 0.5338353152862894 1102 42-43 1.1578150023376779 1102 44-45 0.5352655926785062 1102 46-47 0.5156458588258488 1102 48-49 0.06235472411011855 1102 50-51 -0.9169036356459301 1102 52-53 -0.6330567227377237 1102 54-55 -0.34029809938714806 1102 56-57 -0.6012454389556297 1102 58-59 -0.33732316088640246 1102 60-61 -0.4150369040862749 1102 62-63 0.04491830914535555 1102 64-65 0.7349176312747758 1102 66-67 -0.3961450687682486 1102 68-69 -0.23592304478637516 1102 70-71 0.24585200268379026 1102 72-73 0.3007186651460998 1102 74-75 0.38272438493454786 1102 76-77 0.09984369356003242 1102 78-79 0.348082434980185 1102 80-81 0.9360754773514621 1102 82-83 1.3325522845138487 1102 84-85 0.811758211104145 1102 86-87 0.2718504650013216 1102 88-89 0.29910062060431386 1102 90-91 -0.021858820460366246 1102 92-93 0.09555168740445907 1102 94-95 0.4339479322522699 1102 96-97 0.4237135407842665 1102 98-99 0.23989411809155925 1102 100-101 0.12513521380580528 1103 1 0.37298461001136474 1103 2 0.43635656085952945 1103 3 0.43561473231417835 1103 4 0.24548719895116733 1103 5 0.2893157339142647 1103 6 0.1262328684220222 1103 7 -0.0450848816603866 1103 8 0.18585480565871393 1103 9 0.14587251238097565 1103 10-11 0.3660383553810931 1103 12-13 0.6013598036583048 1103 14-15 0.5321476775148568 1103 16-17 0.6068222226048263 1103 18-19 0.3926753187763907 1103 20-21 0.4725580635962814 1103 22-23 0.3542152703190027 1103 24-25 0.32319748580228946 1103 26-27 0.19472053460210503 1103 28-29 0.41495623505620216 1103 30-31 0.6843963870397758 1103 32-33 0.7755835605683288 1103 34-35 0.6121006384668632 1103 36-37 0.8434738931933552 1103 38-39 0.008055131344036681 1103 40-41 -0.4177346314476651 1103 42-43 -0.7982054630393876 1103 44-45 -0.8285905889867564 1103 46-47 -0.6108610096911136 1103 48-49 -0.5520621528172711 1103 50-51 -0.0678708349730961 1103 52-53 0.2473049257063451 1103 54-55 -0.11679095052255661 1103 56-57 -0.037466352888905874 1103 58-59 -0.2930632786324061 1103 60-61 0.034535565776355526 1103 62-63 -0.464157945410296 1103 64-65 -0.6200375131042541 1103 66-67 -0.6258198655162168 1103 68-69 -0.47334244484244437 1103 70-71 -0.3073439603103232 1103 72-73 -0.09022610637057937 1103 74-75 0.2617543821310697 1103 76-77 0.18987032658498393 1103 78-79 0.5501780335223785 1103 80-81 0.5770903357759103 1103 82-83 -0.10976619039503888 1103 84-85 0.452282461174228 1103 86-87 0.4729298033809144 1103 88-89 0.11347544538704213 1103 90-91 0.7094980620739797 1103 92-93 0.5486285026553688 1103 94-95 0.7624452689497758 1103 96-97 0.7791592935176546 1103 98-99 0.29189860365366016 1103 100-101 0.8255136831077436 1104 1 -0.03600551294771748 1104 2 -0.18643454308456597 1104 3 0.04382544252045051 1104 4 -0.11586467903042319 1104 5 0.11314157547846548 1104 6 -0.06727650468823754 1104 7 0.18685760305371701 1104 8 0.1908269214955709 1104 9 -0.3254706308025206 1104 10-11 -0.1153498056079485 1104 12-13 0.1771845701704322 1104 14-15 0.1368308731071437 1104 16-17 0.2785617912401861 1104 18-19 -0.13285716795343205 1104 20-21 0.18498163411297952 1104 22-23 0.18183738951540107 1104 24-25 -0.2852147966676384 1104 26-27 6.064447008853335E-4 1104 28-29 0.15412172588466433 1104 30-31 -0.058330213779946405 1104 32-33 -0.43704158221202505 1104 34-35 -0.0740177516426499 1104 36-37 -0.30347228701224793 1104 38-39 -0.007936268239376432 1104 40-41 0.1917862984723797 1104 42-43 0.14901619925987575 1104 44-45 -0.225385589994616 1104 46-47 -0.1871897068623838 1104 48-49 -0.6145831499280696 1104 50-51 -0.678231649325042 1104 52-53 -0.5788389968899033 1104 54-55 -0.3739971589482778 1104 56-57 -0.5094814036179258 1104 58-59 0.2680131172555207 1104 60-61 -0.6139895971612255 1104 62-63 -0.24679585443401209 1104 64-65 0.24991478146001356 1104 66-67 1.110053278339187 1104 68-69 0.784132526601482 1104 70-71 0.5195054652087272 1104 72-73 0.47612476374968793 1104 74-75 1.0652550204432387 1104 76-77 0.6404179312345839 1104 78-79 0.9304777042876822 1104 80-81 0.8418890994660231 1104 82-83 1.7032419396862615 1104 84-85 0.9072982510015457 1104 86-87 0.6197416020774114 1104 88-89 -0.04996606506111689 1104 90-91 0.6360921627257277 1104 92-93 1.1104775922206436 1104 94-95 0.42276240931126097 1104 96-97 0.8925436918244465 1104 98-99 1.1123649074902495 1104 100-101 1.3715729453532575 1105 1 0.30847583408247914 1105 2 -0.3567442673450145 1105 3 -0.07141360899727545 1105 4 0.29419170468781175 1105 5 0.2936467118034827 1105 6 0.13261536215350134 1105 7 0.24949759687468287 1105 8 0.09064927416414292 1105 9 0.5079270675059675 1105 10-11 0.380209011106345 1105 12-13 0.6658305885530709 1105 14-15 0.7268517273590973 1105 16-17 0.7866717781097279 1105 18-19 0.6990730089215234 1105 20-21 0.7914927949996695 1105 22-23 0.5657873393107238 1105 24-25 0.5527013228967377 1105 26-27 0.6208659642807035 1105 28-29 0.6019860960090213 1105 30-31 0.7922529328949608 1105 32-33 0.9127691847610109 1105 34-35 1.1573099690648476 1105 36-37 1.518764524604343 1105 38-39 2.0830114416548042 1105 40-41 1.8160621925018816 1105 42-43 1.6121736874810821 1105 44-45 2.3527406168486706 1105 46-47 2.404221430852914 1105 48-49 2.7000843406102746 1105 50-51 3.0038182464036964 1105 52-53 2.5958346726860597 1105 54-55 2.63110054708401 1105 56-57 1.8745844031326726 1105 58-59 2.147328788839669 1105 60-61 2.60208362443451 1105 62-63 2.5022579804312066 1105 64-65 1.801586983514543 1105 66-67 1.652920351792499 1105 68-69 1.7858703809306746 1105 70-71 1.7103218705535959 1105 72-73 1.1408733541567315 1105 74-75 0.7364143623757329 1105 76-77 0.7284692172467864 1105 78-79 1.093449821380446 1105 80-81 1.463307188598641 1105 82-83 1.189223248097477 1105 84-85 0.6154159616681127 1105 86-87 1.4225916831772807 1105 88-89 1.391873743388718 1105 90-91 0.984667122175793 1105 92-93 1.458627742834686 1105 94-95 1.8296134172667742 1105 96-97 1.5058517940875227 1105 98-99 1.4434068477080615 1105 100-101 1.8029470088428674 1106 1 0.6011390175396869 1106 2 0.3947459085325349 1106 3 0.29241007192111823 1106 4 0.6033388089633291 1106 5 0.514781453138383 1106 6 0.43984915419117954 1106 7 0.4814499112465995 1106 8 0.38897880540264396 1106 9 0.6660696828566159 1106 10-11 0.5431717015177071 1106 12-13 0.5041189099895291 1106 14-15 0.5964521394571207 1106 16-17 0.614216115441387 1106 18-19 0.6578632068757457 1106 20-21 0.6740816536353336 1106 22-23 0.5606361140549936 1106 24-25 0.5179673468867279 1106 26-27 0.6590954145713894 1106 28-29 0.5812708115877925 1106 30-31 0.7610880200978372 1106 32-33 0.7201133601970255 1106 34-35 0.592735681037766 1106 36-37 0.19828472476623915 1106 38-39 0.3141174833061413 1106 40-41 0.20034359657871192 1106 42-43 0.5543327867525534 1106 44-45 0.5101841373431881 1106 46-47 0.6802535156273706 1106 48-49 0.10644242435448348 1106 50-51 0.15029701599674894 1106 52-53 0.09712247899999227 1106 54-55 0.3772555253752756 1106 56-57 0.657239491807335 1106 58-59 0.9685076835339075 1106 60-61 1.62463863216135 1106 62-63 2.035041849737265 1106 64-65 1.919108508277219 1106 66-67 0.8617289469797811 1106 68-69 0.9966658772880201 1106 70-71 0.451730362716372 1106 72-73 0.23216042458134467 1106 74-75 0.6716193075519818 1106 76-77 0.776228434260549 1106 78-79 0.8924416530089658 1106 80-81 0.12369426638214165 1106 82-83 -0.02510451700664973 1106 84-85 0.19941772780248712 1106 86-87 0.13412306885687286 1106 88-89 1.1296395834063766 1106 90-91 1.2824447364226081 1106 92-93 1.308727087893189 1106 94-95 1.0120771747176533 1106 96-97 1.27963941642912 1106 98-99 1.3466006073666072 1106 100-101 0.5212117818210089 1107 1 0.14765627980498408 1107 2 0.05367988793895506 1107 3 0.2696359956037355 1107 4 0.0688142783758039 1107 5 -0.0829169114891144 1107 6 0.11047301851583313 1107 7 -0.11606674532517758 1107 8 0.06196487445170362 1107 9 0.11597882882875155 1107 10-11 -0.02151636631997178 1107 12-13 -0.07065395494082338 1107 14-15 -0.008877963510776965 1107 16-17 0.32811678170426006 1107 18-19 0.14892922746932413 1107 20-21 0.14400897041304717 1107 22-23 0.033077058936889614 1107 24-25 0.23214057523318843 1107 26-27 -0.13830010329657938 1107 28-29 -0.18147903698882573 1107 30-31 -0.07679810952057409 1107 32-33 0.33171239108740025 1107 34-35 0.21072477855442173 1107 36-37 0.12217933409392145 1107 38-39 -0.22061904001306232 1107 40-41 -0.3860585839179578 1107 42-43 -0.13973141676046907 1107 44-45 -0.4148007203188371 1107 46-47 0.7531790153245197 1107 48-49 0.7996889416167505 1107 50-51 0.8551122794734312 1107 52-53 0.594396858164135 1107 54-55 0.7518769669258489 1107 56-57 0.5425211392937044 1107 58-59 -0.02664676831345858 1107 60-61 -0.9027689995769919 1107 62-63 -0.8491798340907195 1107 64-65 -0.7713821034732327 1107 66-67 0.06539339277021128 1107 68-69 0.09172947510752394 1107 70-71 -0.18942651190506865 1107 72-73 0.07631548212222583 1107 74-75 0.5922734565525865 1107 76-77 -0.39518283163890544 1107 78-79 -0.08102897085535687 1107 80-81 -0.3047733290145871 1107 82-83 -0.12368113723681518 1107 84-85 0.08536563815984621 1107 86-87 0.13312367455039364 1107 88-89 -0.0098516340375987 1107 90-91 -0.06482964274153247 1107 92-93 -0.6393952621976666 1107 94-95 -0.2328955690739889 1107 96-97 -0.3882891117356273 1107 98-99 -0.2886204707678566 1107 100-101 -0.007225528899763134 1108 1 0.20429963644834004 1108 2 0.24458897884804287 1108 3 0.24725837322611 1108 4 0.023359732921257148 1108 5 0.03176840319620311 1108 6 -0.21540110735828932 1108 7 -0.17550730476573762 1108 8 0.17175508424191577 1108 9 0.03625854910847437 1108 10-11 -0.3159219607255608 1108 12-13 0.050674716387852925 1108 14-15 0.15335979872698147 1108 16-17 0.188606292193775 1108 18-19 -0.18498685644676272 1108 20-21 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2316 98-99 1.0029695181905574 2316 100-101 0.13581826940719566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 1.0 12 3.0 13 23.0 14 85.0 15 315.0 16 1072.0 17 2566.0 18 3746.0 19 4393.0 20 5720.0 21 7602.0 22 7343.0 23 5229.0 24 5134.0 25 4479.0 26 4436.0 27 4748.0 28 5294.0 29 4635.0 30 2773.0 31 1913.0 32 1875.0 33 2324.0 34 2941.0 35 4709.0 36 6996.0 37 15376.0 38 23378.0 39 8071.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.286245461570985 20.109068109243086 12.953296102419037 17.65139032676689 2 13.440546377911389 23.713589399334538 44.822981729029244 18.022882493724826 3 18.51878849728469 38.27677953128986 33.091810329117614 10.112621642307833 4 8.489995261872654 23.61409775121187 34.85220687392936 33.043700112986116 5 6.751466997120676 45.376681124029595 33.57218354776397 14.29966833108576 6 19.4885477263118 40.3534094388313 19.57821224977766 20.57983058507924 7 17.854382664741824 40.41807019846304 21.841142073873165 19.886405062921973 8 26.507271203119874 33.18730181871196 21.578889820315634 18.726537157852537 9 29.55716732878959 12.862193388489995 20.31854794620403 37.262091336516384 10-11 21.427998687903198 32.07493530633816 24.620767576630097 21.87629842912855 12-13 27.458176914385685 29.396799941684588 25.99482450705252 17.15019863687721 14-15 25.318730181871196 26.862266282756863 28.655829718992603 19.16317381637934 16-17 17.6174508874877 33.45482377810985 29.865510077632397 19.062215256770052 18-19 18.57491708277144 26.32430659328644 38.40215767029923 16.698618653642892 20-21 17.98702482049787 26.016692787112294 37.51940809855305 18.47687429383679 22-23 16.14462222546197 30.054306228815104 35.675547618179834 18.1255239275431 24-25 17.268651820534313 31.137515034442544 35.23453730364107 16.359295841382078 26-27 17.30728578197325 28.669315158362796 36.09578306666181 17.92761599300215 28-29 14.435150256045779 28.746013522132923 40.86490623804057 15.953929983780728 30-31 15.980610125013667 30.290848124794984 36.50909355979152 17.219448190399824 32-33 14.742136530961838 30.578416007581005 40.737325509348686 13.942121952108465 34-35 12.75941247220906 34.129824689288185 38.28479790064511 14.825964937857638 36-37 12.237125050114809 33.86631191456792 36.03637423916609 17.86018879615118 38-39 14.080985530488027 29.834894485548713 38.97875132120859 17.105368662754675 40-41 11.910194263221198 32.4922549841455 38.94339760177862 16.654153150854686 42-43 15.638007070743887 31.981266173415463 37.3276961766957 15.053030579144949 44-45 14.11305900790903 32.5921201297518 36.66107810620694 16.63374275613223 46-47 15.168932463461749 30.367022633669862 35.19809017020811 19.265954732660276 48-49 15.156175966760213 30.057950942158403 34.65393446805409 20.1319386230273 50-51 13.263476327586835 30.16546998578562 35.892043590771586 20.67901009585596 52-53 12.658089441265444 30.99901592739731 36.46025440099136 19.88264023034588 54-55 13.391770237270839 30.558005612858548 33.437329154062034 22.61289499580858 56-57 16.008309946422713 30.907533622480592 29.341035827532163 23.74312060356453 58-59 12.697087874038706 31.46553923533914 34.118161606589645 21.71921128403251 60-61 14.670335678098917 34.69038160148704 29.036337792032658 21.602944928381383 62-63 11.825723116398414 35.13893748542104 30.508295428038256 22.52704397014229 64-65 12.409655680165619 34.19908370904664 30.606086008885907 22.78517460190183 66-67 13.696468272770346 31.364580675729854 31.10361920034989 23.835331851149906 68-69 14.425046470095126 31.12293618106936 30.825163100922115 23.6268542479134 70-71 13.15522834129096 29.68218099646463 33.10493129715348 24.057659365090935 72-73 14.47096985822065 28.021285125924845 32.31402850165834 25.193716514196158 74-75 13.777745380325838 27.28906221525677 32.770346612238946 26.162845792178445 76-77 13.69245908809272 26.594744323358967 34.47352115756096 25.23927543098735 78-79 14.037248970368479 27.104275248751687 34.04599628239239 24.812479498487445 80-81 14.204176841491417 26.180340416226265 33.459197434121805 26.156285308160516 82-83 14.000072894266866 25.678827860188797 33.26128949958086 27.05980974596348 84-85 13.199693844079164 26.707365965666803 31.92367970259139 28.169260487662644 86-87 14.477165870904255 26.766045850493857 29.373838247621826 29.382950030980066 88-89 14.60327295258228 26.046214965192988 28.55304880271167 30.797463279513064 90-91 14.558442978459743 25.402558588766993 27.994678718518788 32.04431971425447 92-93 15.01330320370303 24.400991362029377 27.30692131063892 33.278784123628675 94-95 14.71735248022743 23.61920034989248 27.547472391296424 34.11597477858366 96-97 15.628530816051317 23.682982833400153 26.759120895141596 33.92936545540694 98-99 16.31239794728248 24.14239269885701 25.62507289479823 33.920136459062284 100-101 15.436427519645434 22.85248793573501 25.432636934874836 36.27844760974472 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 195.0 1 123.0 2 145.0 3 274.5 4 203.0 5 107.0 6 154.5 7 282.5 8 412.5 9 394.0 10 269.0 11 181.5 12 162.0 13 173.0 14 196.0 15 208.0 16 221.0 17 253.0 18 288.5 19 341.5 20 409.0 21 500.0 22 621.0 23 714.5 24 884.5 25 1105.5 26 1330.0 27 1606.0 28 2007.0 29 2513.5 30 3057.0 31 4255.0 32 5989.5 33 6883.5 34 6520.5 35 5913.5 36 6716.0 37 7835.0 38 7182.0 39 5984.0 40 5336.0 41 4729.0 42 4308.5 43 4382.0 44 4393.5 45 4029.5 46 3337.0 47 3152.5 48 3602.0 49 3851.5 50 4794.0 51 4837.0 52 3316.0 53 1950.0 54 998.0 55 663.0 56 499.0 57 383.0 58 350.0 59 384.0 60 434.5 61 359.0 62 203.5 63 99.5 64 45.5 65 31.5 66 33.0 67 39.5 68 40.5 69 23.5 70 8.5 71 13.5 72 17.5 73 9.5 74 4.5 75 2.0 76 3.0 77 2.0 78 0.5 79 1.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01676568137915953 2 0.09986514560629807 3 0.0 4 0.0 5 0.0 6 0.00510259868061377 7 0.02259722272843241 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.001822356671647775 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 7.2894266865911E-4 64-65 0.0010934140029886649 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.2894266865911E-4 100-101 0.0021868280059773298 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 137185.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.024929839268143 #Duplication Level Percentage of deduplicated Percentage of total 1 79.28263018259378 22.21890148339833 2 8.299953181085158 4.65211211138244 3 3.2174998699474586 2.7051062433939568 4 1.8701555428393069 2.0964391150636 5 1.1574676169172344 1.6218974377665198 6 0.9233730427092546 1.5526478842439042 7 0.5878374863444832 1.153187301818712 8 0.4915986058367581 1.1021613150125742 9 0.40576392862716537 1.0234355067973904 >10 2.928783228424283 16.907825199548054 >50 0.5098059616084899 10.299959908153223 >100 0.2731103365759767 14.312060356452966 >500 0.028611559069864227 4.883915880016037 >1k 0.02080840659626489 11.085760104967743 >5k 0.002601050824533111 4.384590151984547 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 6015 4.384590151984547 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3390 2.4711156467543827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2417 1.7618544301490686 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 2270 1.6546998578561796 RNA PCR Primer, Index 26 (100% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1871 1.3638517330611948 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1677 1.2224368553413274 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 1423 1.0372854175019135 RNA PCR Primer, Index 26 (100% over 24bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1145 0.8346393556146809 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1015 0.7398768086889965 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 723 0.5270255494405365 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.5219229507599227 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 691 0.5036993840434449 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 636 0.46360753726719395 RNA PCR Primer, Index 26 (100% over 29bp) GGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 625 0.4555891679119437 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 584 0.42570251849692026 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 576 0.4198709771476473 RNA PCR Primer, Index 26 (100% over 27bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 567 0.41331049312971535 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.3943579837445785 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 526 0.38342384371469185 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 515 0.3754054743594416 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 490 0.35718190764296387 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 463 0.3375004555891679 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.31636111819805374 No Hit GTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAAT 394 0.2872034114516893 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 343 0.2500273353500747 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 339 0.2471115646754383 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.2471115646754383 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 329 0.23982213798884716 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 327 0.23836425265152897 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 318 0.23180376863359697 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 317 0.23107482596493784 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 313 0.22815905529030142 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 307 0.22378539927834676 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 299 0.21795385792907385 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 287 0.20920654590516455 RNA PCR Primer, Index 26 (96% over 27bp) CCTTAGTACCTTCTTTATTTCTCTTCCATAGTATGCATGATTAGAGTTGG 266 0.19389874986332326 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 265 0.19316980719466414 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 265 0.19316980719466414 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC 263 0.19171192185734592 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 255 0.18588038050807304 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 246 0.17931989649014105 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 245 0.17859095382148193 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 0.17348835514086816 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 238 0.17348835514086816 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 236 0.17203046980354997 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 226 0.16474104311695886 No Hit TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 225 0.16401210044829972 RNA PCR Primer, Index 26 (95% over 22bp) GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 222 0.1618252724423224 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 216 0.15745161643036773 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 209 0.15234901774975398 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 208 0.15162007508109487 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 204 0.14870430440645843 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 202 0.1472464190691402 No Hit GTACATGGGAGCAAATACAAATAAGCAATCCTAAATTCTATCAATCCCCA 201 0.1465174764004811 No Hit GCTTTATACCAAGTCTTGATATCTAAAAATTAAGTTATCCTACTTTTTTT 198 0.14433064839450377 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 194 0.14141487771986733 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 193 0.14068593505120822 No Hit GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC 187 0.13631227903925355 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 185 0.13485439370193533 No Hit CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA 184 0.13412545103327625 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 183 0.13339650836461714 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 183 0.13339650836461714 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 182 0.132667565695958 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 178 0.1297517950213216 No Hit ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC 178 0.1297517950213216 RNA PCR Primer, Index 26 (96% over 28bp) CGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAA 178 0.1297517950213216 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 175 0.12756496701534425 No Hit GAGTACATGGGATTATAGAAGAGCTAATAAATTTCCATGATCACACACTA 174 0.12683602434668514 No Hit TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 172 0.12537813900936692 RNA PCR Primer, Index 26 (100% over 21bp) CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 166 0.12100448299741226 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 165 0.12027554032875314 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 164 0.11954659766009403 No Hit CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT 162 0.11808871232277582 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 160 0.1166308269854576 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.11444399897948027 No Hit CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 152 0.11079928563618473 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 152 0.11079928563618473 No Hit CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA 152 0.11079928563618473 RNA PCR Primer, Index 26 (100% over 29bp) GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 147 0.10715457229288916 No Hit GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA 146 0.10642562962423006 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 145 0.10569668695557094 No Hit ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 143 0.10423880161825273 No Hit GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA 140 0.1020519736122754 No Hit ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.1020519736122754 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.2894266865911E-4 0.0 5 0.0 0.0 0.0 7.2894266865911E-4 0.0 6 0.0 0.0 0.0 7.2894266865911E-4 0.0 7 0.0 0.0 0.0 7.2894266865911E-4 0.0 8 0.0 0.0 0.0 7.2894266865911E-4 0.0 9 0.0 0.0 0.0 0.00145788533731822 0.0 10-11 0.0 0.0 0.0 0.00364471334329555 0.0 12-13 0.0 0.0 0.0 0.0043736560119546596 0.0 14-15 0.0 0.0 0.0 0.006560484017931989 0.0 16-17 0.0 0.0 0.0 0.012392025367204868 0.0 18-19 0.0 0.0 0.0 0.01968145205379597 0.0 20-21 0.0 0.0 0.0 0.03061559208368262 0.0 22-23 0.0 0.0 0.0 0.06123118416736524 0.0 24-25 0.0 0.0 0.0 0.1246491963407078 0.0 26-27 0.0 0.0 0.0 0.1789554251558115 0.0 28-29 0.0 0.0 0.0 0.2857455261143711 0.0 30-31 0.0 0.0 0.0 0.44939315522834133 0.0 32-33 0.0 0.0 0.0 0.6644312424827787 0.0 34-35 0.0 0.0 0.0 0.9990159273973103 0.0 36-37 0.0 0.0 0.0 1.4013922804971388 0.0 38-39 0.0 0.0 0.0 1.9065495498779021 0.0 40-41 0.0 0.0 0.0 2.611801581805591 0.0 42-43 0.0 0.0 0.0 3.0834274884280353 0.0 44-45 0.0 0.0 0.0 3.4263950140321464 0.0 46-47 0.0 0.0 0.0 3.7864926923497464 0.0 48-49 0.0 0.0 0.0 4.132375988628494 0.0 50-51 0.0 0.0 0.0 4.467325144877355 0.0 52-53 0.0 0.0 0.0 4.820862339177024 0.0 54-55 0.0 0.0 0.0 5.14341947005868 0.0 56-57 0.0 0.0 0.0 5.422968983489449 0.0 58-59 0.0 0.0 0.0 5.65878193680067 0.0 60-61 0.0 0.0 0.0 5.905529030141779 0.0 62-63 0.0 0.0 0.0 6.136968327441046 0.0 64-65 0.0 0.0 0.0 6.3807996501075195 0.0 66-67 0.0 0.0 0.0 6.610416590735139 0.0 68-69 0.0 0.0 0.0 6.914750154900317 0.0 70-71 0.0 0.0 0.0 7.265736049859679 0.0 72-73 0.0 0.0 0.0 7.567882786018879 0.0 74-75 0.0 0.0 0.0 7.858366439479536 0.0 76-77 0.0 0.0 0.0 8.11677661551919 0.0 78-79 0.0 0.0 0.0 8.461930969129277 0.0 80-81 0.0 0.0 0.0 8.797973539381129 0.0 82-83 0.0 0.0 0.0 9.191602580457047 0.0 84-85 0.0 0.0 0.0 9.54222400408208 0.0 86-87 0.0 0.0 0.0 9.882640230345883 0.0 88-89 0.0 0.0 0.0 10.24711156467544 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAT 20 1.7265655E-5 95.129974 1 CTTAGTA 25 4.5714478E-7 95.09525 2 CTATGTC 20 0.002064943 71.29542 4 TGGGAAC 20 0.002064943 71.29542 6 TCCTATG 30 1.29074E-4 63.396835 2 TAAACGC 45 2.0253174E-7 63.373707 4 TACAATG 35 2.7655403E-4 54.340145 2 TTAGTAC 45 1.5032278E-5 52.811424 3 TAGTACC 45 1.5032278E-5 52.811424 4 TATATGG 40 5.345003E-4 47.547626 2 TAATACC 40 5.3546345E-4 47.53028 4 TATGGGA 40 5.3546345E-4 47.53028 4 ATATGGG 40 5.3546345E-4 47.53028 3 ATACCAA 40 5.3546345E-4 47.53028 6 TCTGAGT 20 5.3834094E-4 47.495625 40-41 GTGAGTT 20 5.3834094E-4 47.495625 70-71 GGTCTGC 20 5.3834094E-4 47.495625 60-61 AGGCTTG 20 5.3834094E-4 47.495625 50-51 GTATAAA 55 4.900645E-5 43.2409 1 GGGAAGG 55 4.921819E-5 43.209347 7 >>END_MODULE