FastQCFastQC Report
Fri 10 Feb 2017
SRR2568718_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568718_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113584
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC147140.4725743708857702No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC89160.2863581005041136No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT76350.24521580275335433No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA75140.24132960601030837No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC74510.23930621431764806No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG73390.23570907353069648No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA66920.21492916202035983No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA66170.21252036238624045No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG65410.21007944542366608No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA64590.20744582449036225No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT57910.18599144908247217No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC54820.17606719458990025No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT50310.16158227945672896No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC45660.14664772172518872No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT42980.13804027769926877No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA41980.13482854485377624No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA41350.13280515316111594No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT41220.1323876278912019No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT39240.12602839685712672No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT38950.12509699433193386No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT38080.12230278675635538No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC37950.12188526148644135No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG37450.12027939506369509No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC36620.11761365680193628No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC35720.11472309724099301No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA33650.10807481025082348No Hit
GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG32690.10499154671915066No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT32580.1046382561061465No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC32400.10406014419395784No Hit
GCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCT31860.10232580845739186No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG31450.10100899799073994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.054.766271
GTATCAA79750.044.0777051
TCAACGC92300.036.2328874
ATCAACG93350.035.976273
CAACGCA94700.035.61565
AACGCAG99050.034.0994226
TATCAAC107850.031.62442
ACCCTTG24150.031.0793467
CCCTTGG22850.030.7685818
GTACATG199750.030.7489071
CCTTGGC24750.030.3259099
AACCCTT25750.029.5171686
CTATTAA27900.029.455061
ACATGGG195950.029.2114283
ACGCAGA116500.028.7879497
CGCAGAG116500.028.7471738
CATGGGA100600.026.9158944
GTATAGG38250.026.3282761
ATTAACC33950.026.3044413
CATGGGG84700.025.630954
Produced by FastQC (version 0.11.5)