FastQCFastQC Report
Fri 10 Feb 2017
SRR2568718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113584
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC154610.49656601524159943No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT83150.2670555861027035No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC82380.2645825518116743No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA78730.2528597269256265No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC76430.24547274138099376No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT71450.22947831181044093No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG71120.2284184399714284No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA70190.2254315284251204No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA66470.21348388223988818No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG61910.19883838046444224No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA60710.19498430104985123No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC54140.17388321625496533No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT50490.16216039136891763No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT50160.1611005195299051No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC48490.15573692567793257No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT44480.14285787696750754No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT42030.13498913149605085No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC41270.13254821453347654No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA40880.13129563872373445No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT39870.128051788549787No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT39570.12708826869613923No Hit
GTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTCAG38890.12490429036120432No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG38530.123748066536827No Hit
TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC36850.11835235535639958No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT34210.1098733806442993No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC33920.10894197811910647No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG33550.10775363696627423No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA32580.1046382561061465No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC32480.10431708282159723No Hit
GGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATTGTATG31460.10104111531919487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.053.3267061
GTATCAA82100.042.7576831
TCAACGC97950.034.5841034
CAACGCA99900.034.099275
ATCAACG99750.033.960033
AACGCAG105100.032.4573556
TATCAAC110200.031.0734182
GTACATG200300.030.1119771
GGTTACG1452.4039764E-829.4813489
CCCTTGG21750.029.0445848
ACATGGG197000.028.7958223
ACGCAGA120850.028.1070927
CGCAGAG120900.028.011488
TAGCGGG1205.3794865E-627.7123497
CTTAATA60500.027.6710342
CATGGGG84400.027.3580574
ACTTAAT60800.027.1473161
TAATACT65700.027.117984
ACCCTTG24200.026.5017727
CTATTAA28550.026.3240051
Produced by FastQC (version 0.11.5)