FastQCFastQC Report
Fri 10 Feb 2017
SRR2568717_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568717_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097950
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.12022405391866661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.043.3909651
GTATCAA20550.041.6107641
TCAACGC24500.033.5417374
CAACGCA25150.033.2414675
ATCAACG24900.033.193683
AACGCAG27150.031.1426546
TATCAAC27650.030.066852
GTACATG32050.027.5695691
TACATGG31950.027.0611082
ACGCAGA31300.026.4064737
CCGTAGA908.941105E-426.3920141
CGCAGAG31750.026.1818168
ACATGGG32000.025.828833
GTGTAGC8250.023.0330311
GCAGAGT37550.021.631759
AGAGTAC34100.020.0587410-11
TGGTATC7850.019.9705182
GTGGTAT7450.019.7674431
CCTAGAC4001.8189894E-1219.0005193
CCTATAC4001.8189894E-1219.0005193
Produced by FastQC (version 0.11.5)