##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568717_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1097950 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613911380299648 33.0 31.0 34.0 28.0 34.0 2 31.756252106197913 33.0 31.0 34.0 29.0 34.0 3 31.79228835557175 34.0 31.0 34.0 28.0 34.0 4 35.15499157520834 37.0 35.0 37.0 32.0 37.0 5 35.18802131244592 37.0 35.0 37.0 32.0 37.0 6 35.300458126508495 37.0 35.0 37.0 32.0 37.0 7 35.28423425474748 37.0 35.0 37.0 32.0 37.0 8 35.300480896215674 37.0 35.0 37.0 32.0 37.0 9 37.03640511863018 39.0 37.0 39.0 33.0 39.0 10-11 36.995054419600166 39.0 37.0 39.0 33.0 39.0 12-13 36.960926271688145 39.0 37.0 39.0 33.0 39.0 14-15 38.28859920761419 40.0 38.0 41.0 33.0 41.0 16-17 38.25906052188169 40.0 38.0 41.0 33.0 41.0 18-19 38.24128922082062 40.0 38.0 41.0 33.0 41.0 20-21 38.23088437542693 40.0 38.0 41.0 33.0 41.0 22-23 38.16528120588369 40.0 38.0 41.0 33.0 41.0 24-25 38.104649574206476 40.0 38.0 41.0 32.0 41.0 26-27 37.99901589325562 40.0 38.0 41.0 32.0 41.0 28-29 37.86613916845029 40.0 38.0 41.0 32.0 41.0 30-31 37.76656450658045 40.0 38.0 41.0 32.0 41.0 32-33 37.6392891297418 40.0 38.0 41.0 31.0 41.0 34-35 37.47699166628718 40.0 37.0 41.0 31.0 41.0 36-37 37.357506717063615 40.0 37.0 41.0 30.0 41.0 38-39 37.24354615419645 40.0 37.0 41.0 30.0 41.0 40-41 37.101429937611 40.0 37.0 41.0 30.0 41.0 42-43 36.924701489138855 40.0 36.5 41.0 30.0 41.0 44-45 36.73598615601803 40.0 36.0 41.0 29.0 41.0 46-47 36.77199918029054 40.0 36.5 41.0 29.5 41.0 48-49 36.93615738421604 40.0 37.0 41.0 29.5 41.0 50-51 35.84252561592058 38.5 35.0 40.0 27.5 40.5 52-53 35.99586228881097 39.0 35.5 40.0 28.0 40.5 54-55 36.60720524614054 40.0 36.0 41.0 28.0 41.0 56-57 36.59001867115989 40.0 36.0 41.0 28.0 41.0 58-59 36.431924040256845 39.0 35.0 41.0 28.0 41.0 60-61 36.2277193861287 39.0 35.0 41.0 28.0 41.0 62-63 35.95688146090441 39.0 35.0 41.0 28.0 41.0 64-65 35.78401338858782 39.0 35.0 41.0 28.0 41.0 66-67 35.66286078601029 38.0 35.0 40.5 28.0 41.0 68-69 35.36819436222051 37.0 35.0 40.0 27.5 41.0 70-71 35.00510724532083 37.0 34.0 39.5 26.5 41.0 72-73 34.64119495423289 36.0 34.0 39.0 26.0 41.0 74-75 34.280379798715785 36.0 34.0 39.0 26.0 41.0 76-77 33.912113028826454 35.5 34.0 38.0 26.0 39.5 78-79 33.54491233662735 35.0 33.5 37.0 26.0 39.0 80-81 33.180460403479216 35.0 33.0 37.0 26.0 39.0 82-83 32.86546427432943 35.0 33.0 36.0 25.0 38.0 84-85 32.581059702172226 35.0 33.0 36.0 25.0 37.0 86-87 32.34488546837288 35.0 33.0 35.5 25.0 37.0 88-89 32.131977776765794 35.0 32.5 35.0 24.0 36.5 90-91 31.967808643380845 35.0 32.0 35.0 24.0 36.0 92-93 31.813366728903866 35.0 32.0 35.0 24.0 36.0 94-95 31.67789562366228 35.0 32.0 35.0 23.0 36.0 96-97 31.574529350152556 35.0 32.0 35.0 23.0 35.0 98-99 31.436435174643655 35.0 32.0 35.0 23.0 35.0 100-101 30.119129286397374 34.0 29.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13915714946400115 1101 2 0.24007919919897347 1101 3 0.4482441974331479 1101 4 0.20516560621055646 1101 5 0.11757664828199665 1101 6 0.09898102325753655 1101 7 0.22608377692326798 1101 8 0.1124034260589113 1101 9 0.24347715372673662 1101 10-11 0.2714198883524048 1101 12-13 0.23423161515222546 1101 14-15 0.31997177543711786 1101 16-17 0.37330445533577716 1101 18-19 0.30716778070435424 1101 20-21 0.2857169032360005 1101 22-23 0.08895260107313163 1101 24-25 0.13712592729739015 1101 26-27 0.305933023989752 1101 28-29 0.27233350182653737 1101 30-31 0.2736355971685853 1101 32-33 0.17288212467709485 1101 34-35 0.1416612206744432 1101 36-37 0.06449300072887354 1101 38-39 -0.0345960543593975 1101 40-41 0.24861666106694713 1101 42-43 0.18911227716917978 1101 44-45 0.16372216096555547 1101 46-47 0.12423918620255137 1101 48-49 0.2600551883191926 1101 50-51 -0.03798637529832405 1101 52-53 0.15710965004897304 1101 54-55 0.27234794438872 1101 56-57 0.12241971093043702 1101 58-59 0.18761271707489868 1101 60-61 0.2137901456267386 1101 62-63 0.14193035898605189 1101 64-65 0.1922819189972671 1101 66-67 0.279825150249259 1101 68-69 0.3400180800543353 1101 70-71 0.3535603492366448 1101 72-73 0.06337904983633535 1101 74-75 0.015164949788946558 1101 76-77 0.10146114281607765 1101 78-79 0.027963868651831092 1101 80-81 0.11552172592472942 1101 82-83 -0.10258094183259203 1101 84-85 0.034981976570840345 1101 86-87 0.1552565187672883 1101 88-89 0.15263850101899834 1101 90-91 0.18887037622455694 1101 92-93 0.0931906837739156 1101 94-95 -0.16600036914613625 1101 96-97 -0.024070091446397157 1101 98-99 0.06375397510323921 1101 100-101 0.06668443063513152 1102 1 0.2251628516389026 1102 2 0.1876494671507274 1102 3 0.32258819501097946 1102 4 0.20981439260607715 1102 5 0.3786593045871882 1102 6 0.14775858797469255 1102 7 0.29343756407570964 1102 8 0.4069295904632142 1102 9 0.5268008152294854 1102 10-11 0.3889452453691078 1102 12-13 0.350637352650331 1102 14-15 0.4822692977664289 1102 16-17 0.2990405657460329 1102 18-19 0.4329530912962696 1102 20-21 0.38225018274349054 1102 22-23 0.2896168035255684 1102 24-25 0.36624738866893836 1102 26-27 0.5064016872586663 1102 28-29 0.5467538227631081 1102 30-31 0.4101187934142345 1102 32-33 0.3354193401991168 1102 34-35 0.3325390023770254 1102 36-37 0.3609619415372691 1102 38-39 0.35697431451580997 1102 40-41 0.3419172363306089 1102 42-43 0.19717353770305834 1102 44-45 0.26621307785509885 1102 46-47 0.14552457248705508 1102 48-49 0.3715724462290737 1102 50-51 0.3386599384726807 1102 52-53 0.2924858422072205 1102 54-55 0.32544944814811316 1102 56-57 0.4268962966901384 1102 58-59 0.37876488440598166 1102 60-61 0.5006618971532006 1102 62-63 0.5202576789638513 1102 64-65 0.37547374924402277 1102 66-67 0.4157658829535009 1102 68-69 0.33100120060739613 1102 70-71 0.3516175728325521 1102 72-73 0.453375643669915 1102 74-75 0.3395486859750463 1102 76-77 0.24869268644023634 1102 78-79 0.16816546324233883 1102 80-81 0.1510972418958616 1102 82-83 0.18393126990480368 1102 84-85 0.28957714738377405 1102 86-87 0.0908263577943842 1102 88-89 0.10474086262771465 1102 90-91 0.18354666426007782 1102 92-93 0.10006924763323255 1102 94-95 0.09749183451738475 1102 96-97 0.05901198454895962 1102 98-99 0.049791846627687875 1102 100-101 0.0335783853205065 1103 1 0.19156062651890693 1103 2 0.36873872165229926 1103 3 0.3780185143517727 1103 4 0.22018048310908966 1103 5 0.3038562915194589 1103 6 0.2218887935624636 1103 7 0.32374858147159813 1103 8 0.34599196041021685 1103 9 0.31840736851306417 1103 10-11 0.34651328909465917 1103 12-13 0.28815816112909687 1103 14-15 0.42426219513901486 1103 16-17 0.3951289185468525 1103 18-19 0.404745464295182 1103 20-21 0.3816432687796194 1103 22-23 0.40336461863530104 1103 24-25 0.42715380600378694 1103 26-27 0.5660474575885459 1103 28-29 0.4514457879158442 1103 30-31 0.40926391174511423 1103 32-33 0.40926516324381623 1103 34-35 0.34818697840565704 1103 36-37 0.44490334486107486 1103 38-39 0.3870286940069718 1103 40-41 0.34682630327842645 1103 42-43 0.3757519024340752 1103 44-45 0.2919757086017043 1103 46-47 0.30850178026664565 1103 48-49 0.19369289957646174 1103 50-51 0.2077225858660725 1103 52-53 0.21595919348208525 1103 54-55 0.4530938589907194 1103 56-57 0.33246568603064475 1103 58-59 0.33562370581374523 1103 60-61 0.27278651219635464 1103 62-63 0.27866603842338833 1103 64-65 0.2488871027384647 1103 66-67 0.15046644894412964 1103 68-69 0.2639614841575977 1103 70-71 0.24357301494335815 1103 72-73 0.07735776225049307 1103 74-75 0.22237440802926045 1103 76-77 0.09486427315610513 1103 78-79 0.1709156505186442 1103 80-81 0.31620905278840183 1103 82-83 0.18477227925474438 1103 84-85 0.16591062406441637 1103 86-87 0.2787980166463129 1103 88-89 0.1045587884385526 1103 90-91 0.08955823377426952 1103 92-93 -0.06126062224441853 1103 94-95 0.03302369923452986 1103 96-97 0.17738275002353276 1103 98-99 0.04237882606890864 1103 100-101 -0.3343311255415209 1104 1 0.22094830584044445 1104 2 0.21975804047082192 1104 3 0.2784389066958539 1104 4 0.2942530836733681 1104 5 0.2954501023362752 1104 6 0.0016444819549263912 1104 7 0.1597897653335778 1104 8 0.2354218115820501 1104 9 0.23376279565224678 1104 10-11 0.3365501065787342 1104 12-13 0.24038826294493987 1104 14-15 0.3431360873819784 1104 16-17 0.3608253342711194 1104 18-19 0.3244950357801173 1104 20-21 0.2665836579409202 1104 22-23 0.1952919583938737 1104 24-25 0.3357938964478251 1104 26-27 0.38529408334321147 1104 28-29 0.28725779495773907 1104 30-31 0.36668178107205307 1104 32-33 0.2966904424831327 1104 34-35 0.24361977033465365 1104 36-37 0.23473245272089116 1104 38-39 0.16076445023246322 1104 40-41 0.2561692578939514 1104 42-43 0.10608496722024796 1104 44-45 0.32649645138325667 1104 46-47 0.20808959353022516 1104 48-49 0.29525843005656327 1104 50-51 0.3509075386050924 1104 52-53 0.257409944916823 1104 54-55 0.19822395517039126 1104 56-57 0.43961973263849785 1104 58-59 0.5361778744282049 1104 60-61 0.29175224093719265 1104 62-63 0.3212962423534478 1104 64-65 0.21119808366498205 1104 66-67 0.04394895856706427 1104 68-69 0.11407031206528728 1104 70-71 0.2173904957057715 1104 72-73 0.24234393704485768 1104 74-75 0.1792688590492375 1104 76-77 0.022109128488750684 1104 78-79 0.04570719493005271 1104 80-81 0.027628191912413058 1104 82-83 0.07980598026561125 1104 84-85 0.1559441261514536 1104 86-87 0.0749819117736763 1104 88-89 0.04657953950859195 1104 90-91 4.4740668217357893E-4 1104 92-93 0.31591842245707724 1104 94-95 0.07375915375888198 1104 96-97 0.03223218126717242 1104 98-99 0.05984350880198974 1104 100-101 -0.04869392280830809 1105 1 0.03084532705821985 1105 2 -3.8986194123324935E-4 1105 3 0.19332175969191212 1105 4 0.01652829274102885 1105 5 -0.04720246522195026 1105 6 -0.06156375634764544 1105 7 0.014499835137982586 1105 8 0.1525715800525731 1105 9 0.12982472114813248 1105 10-11 0.16926142042212433 1105 12-13 0.17849594142997915 1105 14-15 0.2598227968988809 1105 16-17 0.23741390293515252 1105 18-19 0.20102278476070268 1105 20-21 0.3592272407731869 1105 22-23 0.23923556196012186 1105 24-25 0.40558033642294333 1105 26-27 0.3756126955486181 1105 28-29 0.5041352571182216 1105 30-31 0.32827138643524023 1105 32-33 0.3369655133242375 1105 34-35 0.32489007235967193 1105 36-37 0.3117398478414941 1105 38-39 0.1623319393471192 1105 40-41 0.3211937931071631 1105 42-43 0.2974029574061632 1105 44-45 0.4667784335520295 1105 46-47 0.39632513157708615 1105 48-49 0.30242409458072217 1105 50-51 0.20916311950217903 1105 52-53 0.3370491736126624 1105 54-55 0.31044318248395797 1105 56-57 0.30644950663808146 1105 58-59 0.29089762009135 1105 60-61 0.3494314171069348 1105 62-63 0.5245805052603529 1105 64-65 0.204477508642519 1105 66-67 0.0767929699016392 1105 68-69 0.19180754338742645 1105 70-71 0.2004743301103531 1105 72-73 0.21141159894271055 1105 74-75 0.2820361546467254 1105 76-77 0.3329875582351036 1105 78-79 0.2946615110541799 1105 80-81 0.3602855974000079 1105 82-83 0.2434883356460773 1105 84-85 0.18736440398682674 1105 86-87 0.13453129320984658 1105 88-89 0.17512885183149507 1105 90-91 -0.005113447557924644 1105 92-93 -0.0573430330685234 1105 94-95 0.06690864577630151 1105 96-97 0.12073311429640654 1105 98-99 -0.0821220727862304 1105 100-101 -0.26468120596540246 1106 1 0.12532480022621684 1106 2 -0.04867905908288961 1106 3 0.13319565628712482 1106 4 0.09579128839746431 1106 5 0.053024520906674866 1106 6 0.011699519538581171 1106 7 -0.07144664097242526 1106 8 0.050284904979008616 1106 9 1.8201412837726139E-4 1106 10-11 0.04427893478389677 1106 12-13 0.003837121197385329 1106 14-15 0.1656067397319987 1106 16-17 0.07456116368897625 1106 18-19 0.09754233078843555 1106 20-21 0.07863595591960149 1106 22-23 0.1394933733654753 1106 24-25 0.2335865014782854 1106 26-27 0.1796395976083076 1106 28-29 0.07104011910505648 1106 30-31 -0.04117936317944526 1106 32-33 0.04464801297395127 1106 34-35 0.1186829825460265 1106 36-37 0.06919255906469601 1106 38-39 0.02517487055071399 1106 40-41 0.16404513120441067 1106 42-43 0.09123930320972562 1106 44-45 0.1747061342666214 1106 46-47 0.26905316856741734 1106 48-49 0.2049545695702122 1106 50-51 0.33452391535477943 1106 52-53 0.4105884758404841 1106 54-55 0.3046144028846882 1106 56-57 0.3560249585086197 1106 58-59 0.1352762105355083 1106 60-61 0.46297632393053334 1106 62-63 0.48596343926624286 1106 64-65 0.482783519571484 1106 66-67 0.34892271629368565 1106 68-69 0.512749115476538 1106 70-71 0.44833477567572544 1106 72-73 0.37010993157547034 1106 74-75 0.5224915280529174 1106 76-77 0.48059159354406233 1106 78-79 0.29108718010279944 1106 80-81 0.40630493325540584 1106 82-83 0.1600695322272756 1106 84-85 0.12410112810059815 1106 86-87 0.28690483951781687 1106 88-89 0.20097444521807262 1106 90-91 0.2753026936778866 1106 92-93 0.2935130689351162 1106 94-95 0.23546826466379045 1106 96-97 0.14424299239644967 1106 98-99 0.022095385529585343 1106 100-101 -0.023191784639916335 1107 1 0.23692822793189805 1107 2 0.16938219350119255 1107 3 0.22729579021590496 1107 4 -0.030606525187984346 1107 5 0.08261951467688533 1107 6 0.06698208437581599 1107 7 0.1696422731156062 1107 8 0.2262705881324507 1107 9 0.3759409049927527 1107 10-11 0.0969257003494306 1107 12-13 0.16603716195155016 1107 14-15 0.11351917737578532 1107 16-17 0.24009931316967226 1107 18-19 0.16951388375563425 1107 20-21 0.23033324265629318 1107 22-23 0.14909082482989788 1107 24-25 0.24820356005385236 1107 26-27 0.24065418977884434 1107 28-29 0.24459377383634973 1107 30-31 0.15334441190109516 1107 32-33 0.013119140511172134 1107 34-35 0.11278996068654834 1107 36-37 0.03306678734092827 1107 38-39 -0.1274856612134343 1107 40-41 -0.15377478514066212 1107 42-43 -0.052444422654708944 1107 44-45 0.10689010667822885 1107 46-47 -0.01026533460347423 1107 48-49 0.02215345147489245 1107 50-51 -0.17178675897118723 1107 52-53 -0.2345924213423416 1107 54-55 -0.32274782235521116 1107 56-57 0.07011501278010002 1107 58-59 0.10048311572295887 1107 60-61 0.016447117034680048 1107 62-63 -0.04896653978113363 1107 64-65 0.11238527977829449 1107 66-67 -0.03498350867244682 1107 68-69 -0.030220090237129682 1107 70-71 -0.007429061687140859 1107 72-73 -0.10292504079102827 1107 74-75 0.0892152038923868 1107 76-77 -0.057509277692986416 1107 78-79 -0.11650623949871175 1107 80-81 -0.13389469995706094 1107 82-83 0.004172388285724082 1107 84-85 0.09991733923830992 1107 86-87 0.0609995758523354 1107 88-89 0.11131138265108831 1107 90-91 0.09037714781311479 1107 92-93 -0.07671678769420254 1107 94-95 -0.12366658030703803 1107 96-97 0.07135563723318938 1107 98-99 0.2318020332813404 1107 100-101 -0.008760997508900914 1108 1 0.20007609628898848 1108 2 -4.901963893928496E-4 1108 3 0.1501556504388475 1108 4 0.13771000957491708 1108 5 0.19657679899209768 1108 6 0.25090001176829446 1108 7 0.14114420526061622 1108 8 0.17250469042046035 1108 9 0.2011067724301796 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1752.0 13 2707.0 14 3220.0 15 3756.0 16 4100.0 17 4567.0 18 5236.0 19 5959.0 20 6801.0 21 7893.0 22 8694.0 23 9022.0 24 9176.0 25 9336.0 26 10051.0 27 11212.0 28 12753.0 29 14621.0 30 16985.0 31 20723.0 32 25671.0 33 32917.0 34 45807.0 35 68044.0 36 117251.0 37 228738.0 38 317565.0 39 91548.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18865621612759 18.808419940430106 13.24665033200652 23.75627351143578 2 14.915206686548022 19.71332955624013 42.090420696527566 23.281043060684286 3 18.968301459919832 25.37686588574754 29.092470697316873 26.56236195701575 4 11.79923566229243 16.774352790306246 39.61759433996634 31.808817207434988 5 13.152384292044466 38.19230086117778 35.228956704298604 13.426358142479152 6 31.092596944342738 37.58518550903636 18.572672506917765 12.749545039703142 7 28.017815749085763 32.22091164536094 21.998260323069847 17.763012282483455 8 25.388236237082324 36.65387270769841 20.00520987982665 17.952681175392605 9 25.96147277508419 16.481323431763055 20.08110227685716 37.4761015162956 10-11 24.56433980584293 26.731072964657148 28.88500866219621 19.819578567303715 12-13 25.467649685470718 23.660307465863564 28.922211783200908 21.94983106546481 14-15 22.31923129468555 25.573386766246188 26.333348513138123 25.774033425930142 16-17 22.367047679766838 28.643471924951047 26.72507855548978 22.26440183979234 18-19 22.017325030579745 28.003304342915747 28.362656188948666 21.616714437555842 20-21 23.014932577975927 26.979056137203024 28.402728758988466 21.603282525832583 22-23 22.445821561351835 27.01289498754948 28.00146819606793 22.539815255030756 24-25 22.18614945329186 27.083115120658324 28.39639869391175 22.33433673213806 26-27 22.325084467421465 27.375368712420023 28.083988784129094 22.215558036029414 28-29 22.221974277619726 27.46058325982282 28.04245864094277 22.27498382161469 30-31 22.579011419035613 27.207564837823462 28.071689609187693 22.141734133953232 32-33 22.191983940922412 27.36846803030386 28.119643406870647 22.31990462190308 34-35 22.574707409262718 27.472562502846216 27.98679356983469 21.965936518056377 36-37 22.486224327155153 27.31777403342593 27.979871578851494 22.216130060567423 38-39 22.445861531756726 27.542685289883817 27.922575603500526 22.08887757485893 40-41 22.711143494694657 27.299831504166853 27.89981328840111 22.089211712737374 42-43 22.416914136448483 27.21004822188351 27.94159637249754 22.431441269170467 44-45 22.600080332039095 27.355518455876126 27.861692273642618 22.182708938442158 46-47 22.497884638330078 27.323732764014895 27.65749994762829 22.52088265002673 48-49 22.402659947272856 27.524315373710113 27.70861900700099 22.36440567201604 50-51 22.592237541698452 27.538897680826114 27.538715516946933 22.330149260528504 52-53 22.65717928867435 27.562138530898494 27.419782321599346 22.360899858827814 54-55 22.52311125278929 27.426613233753816 27.727036750307395 22.323238763149504 56-57 22.52953231021449 27.59442597568195 27.578942574798486 22.297099139305068 58-59 22.626485723393596 27.487408351928593 27.686688829181655 22.199417095496152 60-61 22.729586957511728 27.533494239264083 27.59410719978141 22.14281160344278 62-63 22.582817858774167 27.511017258284063 27.64941324020105 22.256751642740724 64-65 22.42152874804858 27.46175033108119 27.710630484243815 22.406090436626414 66-67 22.383928380318796 27.607760377553543 27.61609439020758 22.39221685192008 68-69 22.589004486886378 27.480994948381678 27.566934398187026 22.363066166544915 70-71 22.511460066688343 27.307291908596348 27.671381847691546 22.509866177023763 72-73 22.509085113165444 27.418598296825902 27.58914340361583 22.483173186392825 74-75 22.462088437542693 27.470194453299328 27.51382121225921 22.553895896898766 76-77 22.424882736008016 27.393688237169272 27.562001912655404 22.619427114167312 78-79 22.455803998360583 27.403843526572246 27.55293956919714 22.587412905870032 80-81 22.506895684777025 27.46969360204338 27.390306689451883 22.633104023727714 82-83 22.586928947014844 27.477449370677466 27.55332788307604 22.38229379923165 84-85 22.67157370694858 27.466823959170757 27.423826626007465 22.437775707873204 86-87 22.486531738093547 27.510416609732857 27.536742803111046 22.46630884906255 88-89 22.495602484653105 27.471695122606643 27.49205441168031 22.54064798105994 90-91 22.532956389574746 27.51211198272144 27.578883315501834 22.37604831220198 92-93 22.536458285891257 27.46887040798666 27.606186750225444 22.388484555896632 94-95 22.354987204820354 27.51551227635766 27.639273412354616 22.490227106467373 96-97 22.419688784075394 27.44030686185296 27.63186779653915 22.5081365575325 98-99 22.341949409418245 27.342981042992932 27.596175349468723 22.718894198120104 100-101 22.540944406336852 27.252135922949183 27.705100071144294 22.50181959956967 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 7.0 2 12.0 3 25.0 4 24.0 5 22.0 6 34.5 7 57.5 8 110.5 9 151.5 10 151.5 11 171.5 12 211.5 13 265.5 14 310.0 15 349.5 16 404.0 17 439.5 18 509.5 19 596.5 20 687.0 21 817.0 22 1011.0 23 1336.5 24 1737.0 25 2360.5 26 3267.5 27 4575.0 28 6268.0 29 8488.5 30 11086.0 31 13994.5 32 17425.0 33 20912.0 34 24128.0 35 27006.5 36 30228.5 37 33197.0 38 35495.5 39 37457.0 40 39145.5 41 40753.0 42 41989.0 43 42938.5 44 44096.5 45 44961.0 46 45174.0 47 45544.0 48 45455.0 49 44744.0 50 43308.0 51 41055.5 52 38660.0 53 36571.5 54 34469.0 55 31384.5 56 27977.5 57 24597.0 58 21453.5 59 18421.0 60 14980.5 61 11706.0 62 8938.5 63 6603.5 64 4782.0 65 3475.0 66 2509.5 67 1813.5 68 1330.0 69 986.0 70 734.0 71 564.0 72 425.5 73 304.0 74 208.0 75 163.5 76 118.0 77 70.5 78 44.0 79 33.0 80 25.0 81 15.5 82 15.0 83 16.0 84 9.5 85 6.0 86 8.0 87 9.0 88 7.0 89 3.0 90 1.5 91 3.0 92 3.5 93 1.5 94 0.0 95 0.0 96 1.0 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007286306298101007 2 0.00947219818753131 3 0.0011840247734414137 4 5.464729723575754E-4 5 0.0031877590054191903 6 0.004736099093765655 7 0.0040985472926818155 8 0.003278837834145453 9 0.007013069811922219 10-11 0.0069219909831959555 12-13 0.0035976137346873716 14-15 0.0 16-17 0.0 18-19 9.107882872626258E-5 20-21 0.0013661824308939387 22-23 3.643153149050503E-4 24-25 0.0013661824308939387 26-27 0.0037797713921398973 28-29 0.0031422195910560595 30-31 0.012204563049319186 32-33 3.643153149050503E-4 34-35 0.0 36-37 0.0 38-39 1.3661824308939386E-4 40-41 0.0 42-43 4.098547292681816E-4 44-45 6.37551801083838E-4 46-47 0.002641286033061615 48-49 0.0030511407623297964 50-51 0.003415456077234847 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0012295641878045447 64-65 0.0029145225192404025 66-67 0.0035976137346873716 68-69 0.007650621613006057 70-71 6.37551801083838E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014754770253654539 82-83 0.012978733093492418 84-85 0.018898856960699487 86-87 0.016758504485632316 88-89 0.0155744797121909 90-91 0.01580217678400656 92-93 0.01092945944715151 94-95 0.02468236258481716 96-97 0.011567011248235348 98-99 0.016075413270185347 100-101 0.016485267999453525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1097950.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.75366592162555 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81443542580293 59.603608720394504 2 13.045260721275056 19.242716021948493 3 3.367038039862593 7.449941962122919 4 1.2264363709412296 3.618167135061212 5 0.559469121548535 2.0631449342077985 6 0.2972311096780324 1.3153130378824587 7 0.18018212163624506 0.9302364402946522 8 0.12209769946947664 0.7204122349176654 9 0.07470546201098933 0.4958821518911277 >10 0.2998648771371708 3.565613293814708 >50 0.01117309446728691 0.5406034985891238 >100 0.001734319335822676 0.18876174888800915 >500 1.238789448793259E-4 0.05034225999759652 >1k 2.477578897586518E-4 0.21525655998972304 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1320 0.12022405391866661 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.107882872626258E-5 0.0 16-17 0.0 0.0 0.0 9.107882872626258E-5 0.0 18-19 0.0 0.0 0.0 9.107882872626258E-5 0.0 20-21 0.0 0.0 0.0 1.8215765745252516E-4 0.0 22-23 0.0 0.0 0.0 5.009335579944441E-4 0.0 24-25 0.0 0.0 0.0 7.286306298101006E-4 0.0 26-27 0.0 0.0 0.0 7.286306298101006E-4 0.0 28-29 0.0 0.0 0.0 8.652488728994945E-4 0.0 30-31 0.0 0.0 0.0 0.0011384853590782821 0.0 32-33 0.0 0.0 0.0 0.002003734231977777 0.0 34-35 0.0 0.0 0.0 0.003460995491597978 0.0 36-37 0.0 0.0 0.0 0.006147820939022724 0.0 38-39 0.0 0.0 0.0 0.01165809007696161 0.0 40-41 0.0 0.0 0.0 0.01707728038617423 0.0 42-43 0.0 0.0 0.0 0.023225101325196958 0.0 44-45 0.0 0.0 0.0 0.03251514185527574 0.0 46-47 0.0 0.0 0.0 0.04303474657315907 0.0 48-49 0.0 0.0 0.0 0.0557402431804727 0.0 50-51 0.0 0.0 0.0 0.0710414864064848 0.0 52-53 0.0 0.0 0.0 0.09012250102463681 0.0 54-55 0.0 0.0 0.0 0.11061523748804591 0.0 56-57 0.0 0.0 0.0 0.13379479939887973 0.0 58-59 0.0 0.0 0.0 0.16102736918803223 0.0 60-61 0.0 0.0 0.0 0.19149323739696708 0.0 62-63 0.0 0.0 0.0 0.2260576528985837 0.0 64-65 0.0 0.0 0.0 0.26376428799125645 0.0 66-67 0.0 0.0 0.0 0.3068445739787786 4.553941436313129E-5 68-69 0.0 0.0 0.0 0.35607268090532357 9.107882872626258E-5 70-71 0.0 0.0 0.0 0.41454528894758413 9.107882872626258E-5 72-73 0.0 0.0 0.0 0.4804863609453982 9.107882872626258E-5 74-75 0.0 0.0 0.0 0.5477480759597432 9.107882872626258E-5 76-77 0.0 0.0 0.0 0.6225237943440047 9.107882872626258E-5 78-79 0.0 0.0 0.0 0.7059975408716244 9.107882872626258E-5 80-81 0.0 0.0 0.0 0.8076870531444966 9.107882872626258E-5 82-83 0.0 0.0 0.0 0.9196229336490733 9.107882872626258E-5 84-85 0.0 0.0 0.0 1.0397103693246503 9.107882872626258E-5 86-87 0.0 0.0 0.0 1.1596611867571385 9.107882872626258E-5 88-89 0.0 0.0 0.0 1.2981465458354204 9.107882872626258E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1270 0.0 43.390965 1 GTATCAA 2055 0.0 41.610764 1 TCAACGC 2450 0.0 33.541737 4 CAACGCA 2515 0.0 33.241467 5 ATCAACG 2490 0.0 33.19368 3 AACGCAG 2715 0.0 31.142654 6 TATCAAC 2765 0.0 30.06685 2 GTACATG 3205 0.0 27.569569 1 TACATGG 3195 0.0 27.061108 2 ACGCAGA 3130 0.0 26.406473 7 CCGTAGA 90 8.941105E-4 26.392014 1 CGCAGAG 3175 0.0 26.181816 8 ACATGGG 3200 0.0 25.82883 3 GTGTAGC 825 0.0 23.033031 1 GCAGAGT 3755 0.0 21.63175 9 AGAGTAC 3410 0.0 20.05874 10-11 TGGTATC 785 0.0 19.970518 2 GTGGTAT 745 0.0 19.767443 1 CCTAGAC 400 1.8189894E-12 19.000519 3 CCTATAC 400 1.8189894E-12 19.000519 3 >>END_MODULE