FastQCFastQC Report
Fri 10 Feb 2017
SRR2568717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097950
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.14472425884603124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.12377612823899085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.039.6936651
GTATCAA22950.039.5822561
TCAACGC25500.034.0930564
ATCAACG25850.033.6314473
CAACGCA26600.032.6831935
AACGCAG27950.031.6144896
TATCAAC27600.031.5320532
TACATGG29900.030.2198732
GTACATG31950.030.0697861
ACGCAGA31100.028.5651327
ACATGGG30250.028.4255083
CGCAGAG31500.027.74498
GTATAGC5650.022.7282071
GCAGAGT38300.022.570889
TGGTATC8300.022.3458442
GTGGTAT9900.021.1381661
GTATAGG4500.020.0812591
AGAGTAC32550.020.06446610-11
TAGCCCT11850.020.0450294
CATGGGG18200.019.0548934
Produced by FastQC (version 0.11.5)