FastQCFastQC Report
Fri 10 Feb 2017
SRR2568716_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568716_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791636
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68900.040.4703141
GGTATCA39550.037.7138521
TCAACGC79950.033.746184
CAACGCA82450.032.8957865
ATCAACG84750.031.8905243
AACGCAG88400.030.735386
TATCAAC89100.030.5488342
GTACATG142200.026.2233281
ACGCAGA102550.026.1239177
CGCAGAG103200.025.8212938
TACATGG142150.025.263452
ACATGGG140350.024.2320233
GCAGAGT123450.021.2394269
GTATATA23250.020.6355551
CATGGGG90500.020.5221584
GTATAGA14650.019.1307541
AGAGTAC107350.018.8713610-11
TAATACT29450.018.5484394
GTATTAT20250.017.5935691
GTATAAA23550.017.5487441
Produced by FastQC (version 0.11.5)