FastQCFastQC Report
Fri 10 Feb 2017
SRR2568716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791636
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41900.042.5770261
GTATCAA70600.039.621481
TCAACGC81200.033.470274
ATCAACG84100.032.4291153
CAACGCA84450.032.2947165
TATCAAC89100.030.9101832
AACGCAG90350.030.3961726
ACGCGAG504.9585346E-528.49846814-15
ACGCAGA102450.026.341727
CGCAGAG102650.025.9581788
GTACATG130600.025.2434161
TACATGG131250.024.1365742
ACATGGG130500.023.0833033
CGTATAC3550.021.4146423
GCAGAGT128000.020.5203829
GTATAGG13250.020.465251
CATGGGG78600.019.4044674
AGAGTAC108950.018.5935410-11
GTATTAT19250.017.2991751
CAGAGTA121350.017.14370210-11
Produced by FastQC (version 0.11.5)