FastQCFastQC Report
Fri 10 Feb 2017
SRR2568715_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568715_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091770
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.11394341298991545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG901.1967131E-842.221555
GGTATCA11500.038.825471
GTATCAA18550.035.0803031
TCAACGC19050.033.660884
CAACGCA20100.031.666165
ATCAACG20000.031.5869943
AACGCAG20450.031.1241996
TATCAAC22600.027.7429222
CGCAGAG23000.027.6734738
ACGCAGA23200.027.4349087
AGGTCGT1701.12289854E-725.146666
ACATGGG27100.025.0641783
GTACATG28900.024.8179451
GGACCGT1351.3428138E-524.6292366
TACATGG28850.024.5316722
GTGTACG1401.7795941E-523.749621
GTGTAGC7450.023.590231
CATGGGG17100.023.055194
GGTCGTT1652.4300334E-623.0299367
GTATAGG4550.022.9666671
Produced by FastQC (version 0.11.5)