FastQCFastQC Report
Fri 10 Feb 2017
SRR2568715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091770
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.14142172801963784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.12310285133315625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.045.4628641
GTATCAA22150.034.8003041
ATCAACG23500.032.3561063
TCAACGC23650.032.1508874
CAACGCA24550.030.9722375
AACGCAG25100.030.1042296
TATCAAC26600.028.7942852
ACGCAGA29000.025.890677
CGCAGAG29100.025.625488
ACCGTAT950.001226980924.9983738
GGACCGT1452.325475E-522.942156
TGGTATC8700.022.4195382
GTACATG29100.021.7470531
GTAGGAC3500.021.7248123
TAGCCCT8100.021.708054
GCAGAGT34550.021.308319
TACGACT1353.783373E-421.1213464
GCACCGT1150.003712719820.6621866
ACATGGG26700.020.1127413
TACATGG29200.019.7135372
Produced by FastQC (version 0.11.5)