##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568715_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1091770 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.294145287011 33.0 31.0 34.0 30.0 34.0 2 32.422990190241535 34.0 31.0 34.0 31.0 34.0 3 32.47387636590124 34.0 31.0 34.0 30.0 34.0 4 35.99274022916915 37.0 35.0 37.0 35.0 37.0 5 36.03381573041941 37.0 35.0 37.0 35.0 37.0 6 36.037071910750434 37.0 35.0 37.0 35.0 37.0 7 35.98149518671515 37.0 35.0 37.0 35.0 37.0 8 35.975449957408614 37.0 35.0 37.0 35.0 37.0 9 37.75641481264369 39.0 38.0 39.0 35.0 39.0 10-11 37.64531265742785 39.0 37.0 39.0 35.0 39.0 12-13 37.62534324995191 39.0 37.0 39.0 35.0 39.0 14-15 38.9647008985409 40.0 38.0 41.0 36.0 41.0 16-17 38.936111543640145 40.0 38.0 41.0 35.0 41.0 18-19 38.95759638018997 40.0 38.5 41.0 35.0 41.0 20-21 38.939225294704926 40.0 39.0 41.0 35.0 41.0 22-23 38.89792447127142 40.0 38.0 41.0 35.0 41.0 24-25 38.86637432792621 40.0 38.0 41.0 35.0 41.0 26-27 38.74273702336573 40.0 38.0 41.0 34.5 41.0 28-29 38.605131575331804 40.0 38.0 41.0 34.0 41.0 30-31 38.52561482729879 40.0 38.0 41.0 34.0 41.0 32-33 38.38406257728276 40.0 38.0 41.0 34.0 41.0 34-35 38.26225945025051 40.0 38.0 41.0 34.0 41.0 36-37 38.166712311201074 40.0 38.0 41.0 33.5 41.0 38-39 38.01914322613737 40.0 38.0 41.0 33.0 41.0 40-41 37.91713318739295 40.0 37.5 41.0 33.0 41.0 42-43 37.77424457532264 40.0 37.0 41.0 33.0 41.0 44-45 37.63259752511976 40.0 37.0 41.0 32.5 41.0 46-47 37.427438471472925 40.0 37.0 41.0 31.5 41.0 48-49 37.25672852340695 40.0 37.0 41.0 31.0 41.0 50-51 37.25323969334201 39.5 36.5 41.0 31.5 41.0 52-53 37.387728642479644 40.0 37.0 41.0 32.0 41.0 54-55 37.274535845461955 40.0 36.5 41.0 31.0 41.0 56-57 37.13320296399425 39.0 36.0 41.0 31.0 41.0 58-59 36.94659726865548 39.0 36.0 41.0 31.0 41.0 60-61 36.90799023603873 39.0 36.0 41.0 31.0 41.0 62-63 36.732265495479815 39.0 35.0 41.0 31.0 41.0 64-65 36.54978979089002 39.0 35.0 40.5 30.0 41.0 66-67 36.32780713886625 38.0 35.0 40.0 30.0 41.0 68-69 36.071199520045425 38.0 35.0 40.0 30.0 41.0 70-71 35.747994998946666 37.0 34.5 40.0 29.5 41.0 72-73 35.37356540296949 37.0 34.0 39.0 29.0 41.0 74-75 35.01667109372853 36.0 34.0 39.0 29.0 41.0 76-77 33.103901920734216 34.5 31.5 36.5 26.5 39.0 78-79 34.22865026516574 35.0 33.5 37.5 28.5 39.0 80-81 34.17938897386812 35.0 34.0 37.0 29.0 39.0 82-83 33.91519917198677 35.0 34.0 37.0 29.0 39.0 84-85 33.58611520741548 35.0 34.0 36.0 29.0 37.5 86-87 33.319415261456165 35.0 33.5 36.0 29.0 37.0 88-89 33.08935032103832 35.0 33.0 36.0 28.0 37.0 90-91 32.91024620570266 35.0 33.0 35.0 27.5 36.0 92-93 32.75897991335171 35.0 33.0 35.0 27.0 36.0 94-95 32.61792822664114 35.0 33.0 35.0 27.0 36.0 96-97 32.49672275296079 35.0 33.0 35.0 27.0 36.0 98-99 32.38151945922676 35.0 33.0 35.0 26.5 35.0 100-101 31.1215402511518 34.0 31.0 35.0 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1835384745144637 1101 2 0.12573920609658984 1101 3 0.15600521704448767 1101 4 0.025795099689077006 1101 5 0.13239541371115848 1101 6 0.1898192799319105 1101 7 0.13375148348873012 1101 8 0.124201113486059 1101 9 0.17858830863804798 1101 10-11 0.20991223660105618 1101 12-13 0.1478900436490349 1101 14-15 0.25347591015242443 1101 16-17 0.21321463154530562 1101 18-19 0.2805423798843947 1101 20-21 0.31564120818244845 1101 22-23 0.24783761929222692 1101 24-25 0.31871950869052057 1101 26-27 0.33448681359853083 1101 28-29 0.19867879246077536 1101 30-31 0.12656808357598237 1101 32-33 0.11152695068329876 1101 34-35 0.2373248832221364 1101 36-37 0.01179515865887737 1101 38-39 0.15079296471851222 1101 40-41 0.2671749838843738 1101 42-43 0.1585181736502861 1101 44-45 -0.03488996261626198 1101 46-47 0.004279421901273395 1101 48-49 -0.04525130703155611 1101 50-51 0.05652841793355634 1101 52-53 0.18902847582903348 1101 54-55 0.14835002951826226 1101 56-57 0.016980122671590436 1101 58-59 0.07462288379240078 1101 60-61 0.17004169639297828 1101 62-63 0.25930952505515137 1101 64-65 0.08841260596835099 1101 66-67 0.043347082551434823 1101 68-69 0.08633404937575762 1101 70-71 -0.02175492586324168 1101 72-73 0.09479238019221015 1101 74-75 -0.1737623177259806 1101 76-77 -0.09199385846559238 1101 78-79 0.22715235841447878 1101 80-81 0.1915255547838015 1101 82-83 0.3506772637122282 1101 84-85 0.4438199647697516 1101 86-87 0.2772458731800995 1101 88-89 0.19274144293989792 1101 90-91 0.25213590518814755 1101 92-93 0.15457589301610852 1101 94-95 0.04959849496700741 1101 96-97 0.08244785981553093 1101 98-99 -0.0011295702363440796 1101 100-101 -0.044076988051219956 1102 1 0.20639085597881746 1102 2 0.05738454889127098 1102 3 0.06474442071434794 1102 4 0.17835934009333698 1102 5 0.12474569862676077 1102 6 0.07036617453105976 1102 7 0.14571883466960855 1102 8 0.15049667310330506 1102 9 0.0909651139994807 1102 10-11 0.1637993634332986 1102 12-13 0.16074977199365748 1102 14-15 0.07033789631879017 1102 16-17 0.18713320660892663 1102 18-19 0.2460250340792811 1102 20-21 0.28197905715925486 1102 22-23 0.20235774257646 1102 24-25 0.14693556072793967 1102 26-27 0.22133813782890144 1102 28-29 0.23063234584213177 1102 30-31 0.14708469469307062 1102 32-33 0.2746836901020018 1102 34-35 0.13744135815314706 1102 36-37 0.14097302483813934 1102 38-39 0.15530905617821844 1102 40-41 0.27546710301134425 1102 42-43 0.25172723719184376 1102 44-45 0.21262867337269853 1102 46-47 0.152681923424403 1102 48-49 -0.09157159517261704 1102 50-51 -0.062737431616668 1102 52-53 0.10622107160726557 1102 54-55 0.09719155193520379 1102 56-57 0.09051029863832838 1102 58-59 0.02899114168582173 1102 60-61 0.12681292170925218 1102 62-63 0.23040940678830424 1102 64-65 0.11111939376895208 1102 66-67 0.08570498313560648 1102 68-69 0.11247684764116883 1102 70-71 0.08384707033788175 1102 72-73 0.08857485877874893 1102 74-75 0.029504356108375873 1102 76-77 -0.008309308417210559 1102 78-79 0.14937801515389992 1102 80-81 0.31933976830922717 1102 82-83 0.2533987452733584 1102 84-85 0.2593837034358515 1102 86-87 0.36542949142903325 1102 88-89 0.18061192281660965 1102 90-91 0.17854525185338588 1102 92-93 0.06721924677734847 1102 94-95 0.19244738218343116 1102 96-97 0.09377325493433375 1102 98-99 0.13235383656579813 1102 100-101 0.03195336710772523 1103 1 0.32597077811956154 1103 2 0.0865934108786206 1103 3 0.12570744403332412 1103 4 0.1669779202612105 1103 5 0.21174371981172158 1103 6 0.22271941241567106 1103 7 0.29037409404957515 1103 8 0.21160972422915592 1103 9 0.10939008069589562 1103 10-11 0.2219028050194609 1103 12-13 0.19950350652204207 1103 14-15 0.27142430457549693 1103 16-17 0.30099913865667105 1103 18-19 0.32693140896772377 1103 20-21 0.328774799473571 1103 22-23 0.2776070344710817 1103 24-25 0.29941166621300397 1103 26-27 0.3383145601830435 1103 28-29 0.33881015466014475 1103 30-31 0.30489454738128785 1103 32-33 0.3045663838771375 1103 34-35 0.17064665051336902 1103 36-37 0.24047508772048332 1103 38-39 0.2734740608989128 1103 40-41 0.2184565363977029 1103 42-43 0.2642532657229921 1103 44-45 0.28533913263886035 1103 46-47 0.1586571688362639 1103 48-49 0.30925226442204945 1103 50-51 0.396276523556061 1103 52-53 0.525317283405144 1103 54-55 0.304951610911651 1103 56-57 0.4128476284020408 1103 58-59 0.21892634519336696 1103 60-61 0.33475922210113396 1103 62-63 0.15106539044044354 1103 64-65 0.211460836363905 1103 66-67 0.3263405319657764 1103 68-69 0.26372427712163926 1103 70-71 0.07518296449067208 1103 72-73 0.1762492984378241 1103 74-75 0.30950021741025324 1103 76-77 0.012585943720040405 1103 78-79 0.21742855900470204 1103 80-81 0.2919959071625513 1103 82-83 0.1350630192551705 1103 84-85 0.20439851221505734 1103 86-87 0.1647323794643185 1103 88-89 0.2753247359673239 1103 90-91 0.37689494597772466 1103 92-93 0.28573086746566645 1103 94-95 0.1661110353605295 1103 96-97 0.23885273087168457 1103 98-99 0.19840875191648877 1103 100-101 0.2930302046250226 1104 1 0.19363119297798193 1104 2 0.09826177572257677 1104 3 0.1113069006165901 1104 4 0.15507810014221235 1104 5 0.15641332998568913 1104 6 0.08510667231227131 1104 7 0.007510589766397402 1104 8 0.14290776063305088 1104 9 0.156564187894908 1104 10-11 0.12290284275673002 1104 12-13 0.14682604699525825 1104 14-15 0.20821814585251985 1104 16-17 0.24055786414316316 1104 18-19 0.11190065309014585 1104 20-21 0.26001622996779616 1104 22-23 0.02352952211212056 1104 24-25 0.2370571119530922 1104 26-27 0.10203143000203596 1104 28-29 -0.0620552868030515 1104 30-31 -0.018720180218501525 1104 32-33 -0.1423913629560758 1104 34-35 -0.29137078410680317 1104 36-37 -0.32505694493483617 1104 38-39 -0.011492667405299528 1104 40-41 -0.1990585899592645 1104 42-43 -0.1965138072483157 1104 44-45 -0.25508629221993573 1104 46-47 -0.1426296721765965 1104 48-49 -0.12696293866738273 1104 50-51 -0.17693127044875467 1104 52-53 0.09765460431033546 1104 54-55 0.012774547626243304 1104 56-57 -0.10010003000016354 1104 58-59 -0.1878588165108468 1104 60-61 -0.2669615975524451 1104 62-63 -0.11891209298659788 1104 64-65 -0.2784104795348057 1104 66-67 -0.22234055033921862 1104 68-69 -0.18819843694304694 1104 70-71 -0.21493741741053185 1104 72-73 -0.1214965284487235 1104 74-75 -0.09935923640561128 1104 76-77 -0.07868039337444088 1104 78-79 -0.009860521086373808 1104 80-81 -0.07606829651809477 1104 82-83 -0.1503313981442318 1104 84-85 -0.011482590216473909 1104 86-87 -0.10701246625313132 1104 88-89 -0.26979646357479226 1104 90-91 -0.21300404392126637 1104 92-93 -0.11513331608842492 1104 94-95 -0.18858033664092488 1104 96-97 -0.3306656157325847 1104 98-99 -0.12331533830075614 1104 100-101 -0.14691344104713622 1105 1 0.40307598768958286 1105 2 0.2899891022925729 1105 3 0.3281522035339961 1105 4 0.26931932594990116 1105 5 0.2516742153337006 1105 6 0.24925905763161893 1105 7 0.2500718705978713 1105 8 0.23486817407566463 1105 9 0.3703382846832781 1105 10-11 0.4445073210815593 1105 12-13 0.3473734190363018 1105 14-15 0.4167396681772786 1105 16-17 0.39860461525606183 1105 18-19 0.390652491992725 1105 20-21 0.381800299817435 1105 22-23 0.23307748642308468 1105 24-25 0.3091755277265804 1105 26-27 0.2956181975456431 1105 28-29 0.4061111601505729 1105 30-31 0.2853043894305287 1105 32-33 0.5226088280987327 1105 34-35 0.35473122005819135 1105 36-37 0.29709251724585783 1105 38-39 0.41226929740240337 1105 40-41 0.3891007879662638 1105 42-43 0.5147436200075717 1105 44-45 0.6308511182231129 1105 46-47 0.5510750717144219 1105 48-49 0.4933877424430264 1105 50-51 0.42427810652150555 1105 52-53 0.5989146108061973 1105 54-55 0.4835092489120498 1105 56-57 0.4829543633052751 1105 58-59 0.6997765935883109 1105 60-61 0.5667347399267797 1105 62-63 0.49750381423717727 1105 64-65 0.5282162566758117 1105 66-67 0.33046700014701713 1105 68-69 0.3172920007635369 1105 70-71 0.36635831633548577 1105 72-73 0.567238402102852 1105 74-75 0.3542607746755735 1105 76-77 0.17237900556711594 1105 78-79 0.4134266725094662 1105 80-81 0.3439537703128863 1105 82-83 0.2440964882163712 1105 84-85 0.2806440409139981 1105 86-87 0.25818785471895467 1105 88-89 0.30604752106440003 1105 90-91 0.47416471479561295 1105 92-93 0.3954511205864506 1105 94-95 0.5294265795433262 1105 96-97 0.3789859958147659 1105 98-99 0.24701193859498005 1105 100-101 0.4056858596922446 1106 1 0.14268893913706648 1106 2 0.05308656948629675 1106 3 0.0040539426711347915 1106 4 -1.351998905789742E-4 1106 5 0.053174095898050666 1106 6 0.11484001573667513 1106 7 0.04347517010394597 1106 8 0.08418276757065968 1106 9 -0.08612356911208252 1106 10-11 -0.051391412900571254 1106 12-13 0.006677455961181522 1106 14-15 -0.05237369551583271 1106 16-17 0.04901165964759713 1106 18-19 -0.07133807644389378 1106 20-21 0.10630271549973003 1106 22-23 0.014814620121661903 1106 24-25 -6.443941393854402E-4 1106 26-27 0.007619361080010378 1106 28-29 -0.09813843723571125 1106 30-31 -0.1327858730967293 1106 32-33 -0.21918189753061057 1106 34-35 -0.031973473377469475 1106 36-37 0.08658815206559467 1106 38-39 0.06815111775578231 1106 40-41 -0.04343155597757686 1106 42-43 -0.3017964834005866 1106 44-45 -0.2962603884383057 1106 46-47 -0.10565724603063131 1106 48-49 -0.0744237726944803 1106 50-51 -0.09858418802991764 1106 52-53 -0.1594015777326021 1106 54-55 -0.18817275175063486 1106 56-57 -0.4850908218970389 1106 58-59 -0.3264559052365996 1106 60-61 -0.10313935178438527 1106 62-63 -0.15481612689260515 1106 64-65 -0.14585542012361685 1106 66-67 0.012485000254891077 1106 68-69 -0.1642889034028414 1106 70-71 -0.16828765328933315 1106 72-73 -0.18906760743659135 1106 74-75 -0.109836291830824 1106 76-77 -0.5509791395587555 1106 78-79 -0.35398877861052824 1106 80-81 -0.31572494779587146 1106 82-83 -0.4503389664481716 1106 84-85 -0.3159100480231771 1106 86-87 -0.34168538938438786 1106 88-89 -0.12181720072760527 1106 90-91 -0.127276603773943 1106 92-93 -0.1804540860372228 1106 94-95 -0.4030017398044805 1106 96-97 -0.19133944706031514 1106 98-99 -0.10877914919407061 1106 100-101 0.03001836853433204 1107 1 0.08248455344211436 1107 2 -0.006741676186877044 1107 3 0.013965476491982542 1107 4 0.0695313055732214 1107 5 0.08521194437712865 1107 6 -0.11132272099950313 1107 7 -0.004187144085328498 1107 8 -0.05240490969143252 1107 9 0.04448250481652849 1107 10-11 -0.08873796486896168 1107 12-13 0.04232160796082951 1107 14-15 0.11892633232830718 1107 16-17 0.1610500943189166 1107 18-19 -0.014103731440457068 1107 20-21 -0.11157394965261602 1107 22-23 -0.026552233638035716 1107 24-25 -0.06172684696312558 1107 26-27 0.06633384149386501 1107 28-29 0.039399359951538315 1107 30-31 0.12274875865242052 1107 32-33 0.026554775144525422 1107 34-35 -0.019772126112300725 1107 36-37 0.026237218310200205 1107 38-39 0.007099195777968248 1107 40-41 -6.004428473644907E-4 1107 42-43 -0.09408414747334604 1107 44-45 -0.10313202699217783 1107 46-47 -0.14228327003520747 1107 48-49 0.12614923022183433 1107 50-51 0.04071707081791942 1107 52-53 0.2233416554107066 1107 54-55 0.2589417169393755 1107 56-57 0.17921031482742222 1107 58-59 0.2591489230335 1107 60-61 0.2930350110979205 1107 62-63 0.349670686224421 1107 64-65 0.14438423480454787 1107 66-67 0.2072950006945362 1107 68-69 0.0015778551006064845 1107 70-71 0.14125482366598163 1107 72-73 0.24236621878672082 1107 74-75 0.20878605870236555 1107 76-77 0.09166952123621996 1107 78-79 0.14654367934239332 1107 80-81 0.057944517938743445 1107 82-83 0.18895496499664688 1107 84-85 0.12373657575375319 1107 86-87 0.20149255415552858 1107 88-89 0.22063508566638745 1107 90-91 0.16020550515035126 1107 92-93 0.1724470770658897 1107 94-95 0.19421946472287743 1107 96-97 0.05020717232017091 1107 98-99 0.22094177444950702 1107 100-101 0.2066747279874157 1108 1 -0.0485165992235963 1108 2 -0.1451865496019593 1108 3 -0.11670237524465676 1108 4 -0.06407607519069103 1108 5 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-0.681807156765835 2316 78-79 -0.6680597934007366 2316 80-81 -0.6630166903822712 2316 82-83 -0.7363779741790637 2316 84-85 -0.7364222670852811 2316 86-87 -0.657726477490364 2316 88-89 -0.7882049714730641 2316 90-91 -0.7727210012121404 2316 92-93 -0.7185204123042581 2316 94-95 -0.5782688027197622 2316 96-97 -0.6661505029274686 2316 98-99 -0.6982569191995296 2316 100-101 -0.683063700168308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 3.0 13 10.0 14 22.0 15 88.0 16 224.0 17 519.0 18 1000.0 19 1609.0 20 2433.0 21 3552.0 22 4810.0 23 5319.0 24 5822.0 25 6462.0 26 7893.0 27 9808.0 28 12395.0 29 15475.0 30 19109.0 31 23873.0 32 30005.0 33 38723.0 34 53301.0 35 79072.0 36 132340.0 37 235103.0 38 316009.0 39 86775.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.952893154007796 20.101113364588887 13.679159727604004 22.266833753799308 2 14.528767371950197 20.072481107330898 43.744172678491374 21.654578842227533 3 19.34528334722515 26.454656200481786 29.81891790395413 24.381142548338936 4 11.705121041977707 17.069346107696674 40.80227520448446 30.423257645841158 5 12.775584601152257 38.7962666129313 36.3546351337736 12.07351365214285 6 30.093127336312698 39.38864060835499 18.309913339458163 12.20831871587415 7 26.39163867005161 32.37541697167479 23.39063363203515 17.842310726238455 8 23.720289071874113 39.18270331663262 20.165969022779525 16.93103858871374 9 24.376196451633586 17.008435842714125 20.656090568526338 37.95927713712595 10-11 23.876640684393234 27.252947049286934 29.59661833536368 19.273793930956153 12-13 24.387233574837193 24.436053381206666 29.888575432554475 21.28813761140167 14-15 21.286168332157875 26.703609734651074 26.888996766718265 25.121225166472794 16-17 21.591131831796073 30.016578583401266 27.442318437033443 20.94997114776922 18-19 21.063548183225407 29.09807010634108 29.396759390714163 20.441622319719354 20-21 21.621266383945336 27.970726435055006 29.59707630728084 20.810930873718824 22-23 21.213488188904257 27.984923564487023 29.43380931881257 21.367778927796145 24-25 20.995859933868854 28.238182034677635 28.98041712082215 21.785540910631358 26-27 21.25049231981095 28.25411945739487 28.976936534251717 21.51845168854246 28-29 21.4866563102243 28.180392616638212 29.0693307637632 21.26362030937429 30-31 21.66802531668758 28.017943339714407 29.050624215723094 21.26340712787492 32-33 21.51520008793061 28.11164439396576 29.14574498291765 21.227410535185985 34-35 21.573637304560485 28.161563332936424 29.292204402026066 20.97259496047702 36-37 21.349368456726232 28.0744570742922 29.12362493931872 21.45254952966284 38-39 21.489874240911547 28.18940802549988 29.056119878729035 21.26459785485954 40-41 21.55655495205034 28.1428322815245 28.846414537860536 21.454198228564625 42-43 21.5027432517838 28.03406395119851 28.921613526658547 21.54157927035914 44-45 21.82304881064693 28.21629097703729 28.82301217289356 21.13764803942222 46-47 21.68881724172674 28.225450415380525 28.594529983421417 21.491202359471316 48-49 21.837439129779685 28.045068121064016 28.789959785467538 21.327532963688764 50-51 21.78297626789525 28.417020068329414 28.505637634300264 21.29436602947507 52-53 21.47883711770794 28.352125447667547 28.54113045788032 21.627906976744185 54-55 21.680894327559834 28.16284565430448 28.706870494701263 21.449389523434423 56-57 21.476867838464145 28.582805902342066 28.269598908194947 21.670727350998835 58-59 21.78517453309763 28.299229691235332 28.277384430786707 21.638211344880332 60-61 22.112130811494552 28.256605446479316 28.49914748488577 21.132116257140357 62-63 21.614495793508677 28.28493311289517 28.69688073898687 21.403690354609278 64-65 21.5403004760629 28.174725845933313 28.674832324647877 21.61014135335591 66-67 21.506452824312813 28.193117598028888 28.868030812350586 21.43239876530771 68-69 21.413392930745488 28.297489397950116 28.510629528197327 21.778488143107065 70-71 21.709838152724476 27.818542366982058 28.578409371937312 21.893210108356158 72-73 21.45401503979776 28.214504886560356 28.432362127554338 21.899117946087546 74-75 21.591406614946372 28.078395632779795 28.471839306813706 21.858358445460123 76-77 21.70727350998837 28.25979830916768 28.451688542458577 21.581239638385373 78-79 21.49262207241452 28.19009498337562 28.52285737838556 21.794425565824305 80-81 21.52747373531055 28.254852212462332 28.39146523535177 21.82620881687535 82-83 21.642241497751357 28.18940802549988 28.451963325608876 21.716387151139894 84-85 21.50791833444773 28.252653947259954 28.216153585462138 22.023274132830174 86-87 21.31492896855565 28.187988312556673 28.538382626377352 21.958700092510327 88-89 21.573774696135633 28.069785760737155 28.325059307363272 22.03138023576394 90-91 21.517856325050147 28.07129706806379 28.410333678338844 22.000512928547224 92-93 21.807660954230286 27.93326433223115 28.50055414601977 21.7585205675188 94-95 21.49739482555613 27.92856001198055 28.55630240632295 22.017742756140375 96-97 21.33576669078652 27.82248092546965 28.702107586762782 22.13964479698105 98-99 21.434508720950202 27.67837474553877 28.51866354329104 22.368452990219986 100-101 21.401776493406274 27.78094036450402 28.4028733487061 22.414409793383605 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 3.5 2 13.5 3 24.5 4 25.0 5 25.0 6 33.0 7 81.0 8 165.5 9 231.5 10 243.5 11 230.5 12 246.0 13 295.0 14 341.0 15 373.5 16 410.5 17 454.5 18 505.0 19 571.5 20 643.5 21 748.0 22 896.5 23 1025.5 24 1239.5 25 1626.5 26 2132.5 27 2845.5 28 3779.0 29 5340.5 30 8434.5 31 13228.5 32 20078.0 33 29452.0 34 38927.0 35 46779.5 36 54738.5 37 58990.5 38 59092.5 39 57852.0 40 54127.5 41 49074.5 42 42575.0 43 36772.5 44 33746.0 45 32465.0 46 31898.0 47 31578.0 48 31542.5 49 31544.5 50 30981.0 51 30071.0 52 29347.5 53 28425.0 54 27112.5 55 25308.0 56 23395.0 57 21044.5 58 18758.0 59 16283.0 60 13144.5 61 10402.0 62 8023.0 63 5954.5 64 4317.5 65 3107.0 66 2240.5 67 1664.5 68 1256.0 69 940.5 70 672.0 71 491.0 72 376.5 73 284.0 74 214.0 75 159.0 76 125.0 77 96.5 78 59.0 79 23.0 80 16.5 81 11.0 82 7.5 83 3.5 84 1.0 85 1.0 86 2.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011357703545618582 2 0.09186916658270515 3 0.0 4 0.0 5 0.0 6 0.003572180953863909 7 0.024638889143317733 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0016486989017833428 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 4.579719171620396E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 4.579719171620396E-5 62-63 4.579719171620396E-4 64-65 2.289859585810198E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 4.579719171620396E-5 96-97 0.0 98-99 2.289859585810198E-4 100-101 0.001144929792905099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1091770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.07621117449232 #Duplication Level Percentage of deduplicated Percentage of total 1 75.71128914001476 45.48447394668574 2 13.884228779917054 16.682237203545217 3 4.386543615544167 7.905807617206576 4 2.014715847567047 4.841459788601369 5 1.1304208997625058 3.3955702345095964 6 0.7051552819905197 2.5417834579002685 7 0.4524414547039451 1.9026677863822097 8 0.3456531656060229 1.6612426056063359 9 0.2431286166449742 1.3145621504513094 >10 1.0707485983041218 10.560866692591265 >50 0.038708142417062476 1.5949199156016058 >100 0.016202203835746734 1.6904436540845027 >500 4.585522152237843E-4 0.15876876609836396 >1k 3.057014768158562E-4 0.2651961807357288 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1544 0.14142172801963784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1344 0.12310285133315625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.159438343240793E-5 0.0 18-19 0.0 0.0 0.0 1.373915751486119E-4 0.0 20-21 0.0 0.0 0.0 1.8318876686481585E-4 0.0 22-23 0.0 0.0 0.0 4.579719171620396E-4 0.0 24-25 0.0 0.0 0.0 7.327550674592634E-4 0.0 26-27 0.0 0.0 0.0 9.159438343240792E-4 0.0 28-29 0.0 0.0 0.0 0.001190726984621303 0.0 30-31 0.0 0.0 0.0 0.0019234820520805664 0.0 32-33 9.159438343240793E-5 0.0 0.0 0.004396530404755581 0.0 34-35 9.159438343240793E-5 0.0 0.0 0.007556536633173653 0.0 36-37 9.159438343240793E-5 0.0 0.0 0.012869010872253315 0.0 38-39 9.159438343240793E-5 0.0 0.0 0.023677148117277447 0.0 40-41 9.159438343240793E-5 0.0 0.0 0.03837804665817892 0.0 42-43 9.159438343240793E-5 0.0 0.0 0.0555061963600392 0.0 44-45 9.159438343240793E-5 0.0 0.0 0.07822160345127636 0.0 46-47 9.159438343240793E-5 0.0 0.0 0.1051961493721205 0.0 48-49 9.159438343240793E-5 0.0 0.0 0.13665882008115263 0.0 50-51 9.159438343240793E-5 0.0 0.0 0.17045714756771113 0.0 52-53 9.159438343240793E-5 0.0 0.0 0.21020910997737619 0.0 54-55 9.159438343240793E-5 0.0 0.0 0.2545865887503778 0.0 56-57 9.159438343240793E-5 0.0 0.0 0.30652060415655313 0.0 58-59 9.159438343240793E-5 0.0 0.0 0.3703160922172253 0.0 60-61 9.159438343240793E-5 0.0 0.0 0.44043159273473353 0.0 62-63 9.159438343240793E-5 0.0 0.0 0.5137986938640922 0.0 64-65 9.159438343240793E-5 0.0 0.0 0.5954092895023677 0.0 66-67 9.159438343240793E-5 0.0 0.0 0.6840268554732224 0.0 68-69 9.159438343240793E-5 0.0 0.0 0.788810830119897 0.0 70-71 9.159438343240793E-5 0.0 0.0 0.9137913663134176 0.0 72-73 9.159438343240793E-5 0.0 0.0 1.055579471866785 0.0 74-75 9.159438343240793E-5 0.0 0.0 1.202176282550354 0.0 76-77 9.159438343240793E-5 0.0 0.0 1.3570623849345558 0.0 78-79 1.8318876686481585E-4 0.0 0.0 1.5448308709709921 0.0 80-81 1.8318876686481585E-4 0.0 0.0 1.7626423147732582 0.0 82-83 1.8318876686481585E-4 0.0 0.0 2.0002839425886405 0.0 84-85 1.8318876686481585E-4 0.0 0.0 2.2263388808998235 0.0 86-87 1.8318876686481585E-4 0.0 0.0 2.4824367769768356 0.0 88-89 1.8318876686481585E-4 0.0 0.0 2.7670663234930437 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1235 0.0 45.462864 1 GTATCAA 2215 0.0 34.800304 1 ATCAACG 2350 0.0 32.356106 3 TCAACGC 2365 0.0 32.150887 4 CAACGCA 2455 0.0 30.972237 5 AACGCAG 2510 0.0 30.104229 6 TATCAAC 2660 0.0 28.794285 2 ACGCAGA 2900 0.0 25.89067 7 CGCAGAG 2910 0.0 25.62548 8 ACCGTAT 95 0.0012269809 24.998373 8 GGACCGT 145 2.325475E-5 22.94215 6 TGGTATC 870 0.0 22.419538 2 GTACATG 2910 0.0 21.747053 1 GTAGGAC 350 0.0 21.724812 3 TAGCCCT 810 0.0 21.70805 4 GCAGAGT 3455 0.0 21.30831 9 TACGACT 135 3.783373E-4 21.121346 4 GCACCGT 115 0.0037127198 20.662186 6 ACATGGG 2670 0.0 20.112741 3 TACATGG 2920 0.0 19.713537 2 >>END_MODULE