FastQCFastQC Report
Fri 10 Feb 2017
SRR2568714_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568714_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171353
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC55730.17572941265131947No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT52450.1653868238571991No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC51380.16201286958594643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40850.128809375682871No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA37540.11837219004002392No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC37030.11676404361166985No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT36950.11651178534839862No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC31910.10061951476231122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.052.657131
GTATCAA83000.045.9032821
GTACATG232550.037.09771
TCAACGC103700.036.4150624
ATCAACG106050.035.4295273
CAACGCA106500.035.4130675
ACATGGG234200.035.2299843
TACATGG249050.034.2202642
AACGCAG110550.034.2016456
CATGGGG106900.033.0144274
TATCAAC119450.031.4187132
CATGGGA121950.031.1212944
ATGGGGT40050.030.5991865
GACATAT12700.029.924971
ACGCAGA129500.028.9767727
CGCAGAG129850.028.7157658
ATGGGAT54850.028.491285
ATGGGGG41350.025.8463765
CTATAAC25450.024.4502333
CATTCCG2751.8189894E-1224.1817679
Produced by FastQC (version 0.11.5)