FastQCFastQC Report
Fri 10 Feb 2017
SRR2568714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171353
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC59130.18645038884034668No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT55930.17636005830949755No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC55200.1740582016571476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.16229666013212657No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT38310.12080017582400951No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC36930.11644872078258081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.11562888142694931No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA33450.10547548633028238No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT32710.1031420973950235No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC32310.10188080607866735No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT31900.10058798247940233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.052.8550571
GTATCAA82200.043.6444361
GTACATG225650.037.2800371
ACATGGG227350.035.297763
GCGCGTA557.020935E-834.5456524-25
TACATGG244200.034.105612
TCAACGC107100.033.139324
CAACGCA108400.032.7857255
ATCAACG109500.032.6299363
CATGGGG99750.032.0087624
CATGGGA118800.031.835314
AACGCAG113700.031.6335476
GACATAT12050.030.7990231
ATGGGGT35850.030.0849425
TATCAAC120100.029.8220372
ATGGGAT52050.028.8455525
ACGCAGA135150.026.4714497
CGCAGAG136200.026.0502668
GTGTAGC27100.024.404781
ATGGGGG40050.023.9641135
Produced by FastQC (version 0.11.5)