##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568714_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3171353 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74942461466762 34.0 31.0 34.0 31.0 34.0 2 32.85095635837448 34.0 31.0 34.0 31.0 34.0 3 32.92688073513103 34.0 31.0 34.0 31.0 34.0 4 36.290317098096615 37.0 37.0 37.0 35.0 37.0 5 36.363752001117504 37.0 37.0 37.0 35.0 37.0 6 36.386563085219464 37.0 37.0 37.0 35.0 37.0 7 36.32953411367325 37.0 37.0 37.0 35.0 37.0 8 36.328042952014485 37.0 37.0 37.0 35.0 37.0 9 38.1642844552467 39.0 39.0 39.0 37.0 39.0 10-11 38.0840354258892 39.0 39.0 39.0 36.0 39.0 12-13 38.08695925682193 39.0 39.0 39.0 37.0 39.0 14-15 39.55583389802396 41.0 40.0 41.0 37.0 41.0 16-17 39.54963134031437 41.0 40.0 41.0 37.0 41.0 18-19 39.622773939072694 41.0 40.0 41.0 37.0 41.0 20-21 39.60162933612247 41.0 40.0 41.0 37.0 41.0 22-23 39.56087244150998 41.0 40.0 41.0 37.0 41.0 24-25 39.53637280365825 41.0 40.0 41.0 37.0 41.0 26-27 39.45180511283355 41.0 39.0 41.0 36.5 41.0 28-29 39.36210443933551 41.0 39.0 41.0 36.0 41.0 30-31 39.306389102695285 41.0 39.0 41.0 36.0 41.0 32-33 39.17651330520444 41.0 39.0 41.0 35.5 41.0 34-35 39.0964481090563 41.0 39.0 41.0 35.0 41.0 36-37 39.02520911421718 41.0 39.0 41.0 35.0 41.0 38-39 38.88831974870032 40.0 39.0 41.0 35.0 41.0 40-41 38.83411638502557 40.0 38.5 41.0 35.0 41.0 42-43 38.71813481501429 40.0 38.0 41.0 35.0 41.0 44-45 38.60619899456162 40.0 38.0 41.0 35.0 41.0 46-47 38.42895697829917 40.0 38.0 41.0 34.0 41.0 48-49 38.2948577468355 40.0 38.0 41.0 34.0 41.0 50-51 38.32038297218884 40.0 38.0 41.0 34.0 41.0 52-53 38.41446537171989 40.0 38.0 41.0 34.0 41.0 54-55 38.306263919532135 40.0 38.0 41.0 34.0 41.0 56-57 38.18961686069006 40.0 38.0 41.0 34.0 41.0 58-59 38.01327966328567 40.0 38.0 41.0 33.5 41.0 60-61 37.96563422614891 40.0 37.5 41.0 34.0 41.0 62-63 37.795165028932445 40.0 37.0 41.0 33.0 41.0 64-65 37.58450730650293 39.5 36.5 41.0 33.0 41.0 66-67 37.34793257010494 39.0 36.0 41.0 33.0 41.0 68-69 37.0273686026122 39.0 36.0 41.0 33.0 41.0 70-71 36.643604322823734 38.0 35.0 40.0 32.0 41.0 72-73 36.238754247792656 37.0 35.0 39.5 32.0 41.0 74-75 35.83618206487893 37.0 35.0 39.0 31.5 41.0 76-77 34.02055400329134 35.0 33.0 37.5 29.0 39.0 78-79 34.92609983814479 36.0 34.5 37.5 31.0 39.0 80-81 34.77129398714051 35.5 35.0 37.0 31.0 39.0 82-83 34.49940656243565 35.0 35.0 37.0 31.0 39.0 84-85 34.16373342860287 35.0 34.5 36.0 31.0 37.0 86-87 33.93944083172072 35.0 34.0 36.0 31.0 37.0 88-89 33.750656580960865 35.0 34.0 36.0 31.0 37.0 90-91 33.613192539588 35.0 34.0 35.0 31.0 36.0 92-93 33.497787852692525 35.0 34.0 35.0 30.5 36.0 94-95 33.39075971675181 35.0 34.0 35.0 30.0 36.0 96-97 33.30874708681121 35.0 34.0 35.0 30.0 36.0 98-99 33.238672894502756 35.0 34.0 35.0 30.0 35.5 100-101 32.20235196144989 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2985345631399028 1101 2 0.17555138572005546 1101 3 0.20148953836165617 1101 4 0.2051513994523546 1101 5 0.1397430057974205 1101 6 0.15520901370611995 1101 7 0.20219030003773497 1101 8 0.17792975815628864 1101 9 0.22033673362592765 1101 10-11 0.28697403122884424 1101 12-13 0.2368279930578936 1101 14-15 0.313980210199297 1101 16-17 0.3575715456523909 1101 18-19 0.3000694522712095 1101 20-21 0.3036557215128468 1101 22-23 0.2858525947399997 1101 24-25 0.36679231403668666 1101 26-27 0.30088287391928503 1101 28-29 0.27766554834759916 1101 30-31 0.24547745395220488 1101 32-33 0.23315208049584868 1101 34-35 0.2534149079755039 1101 36-37 0.22004971957986186 1101 38-39 0.17698923737446393 1101 40-41 0.17155842506982566 1101 42-43 0.18394480848753147 1101 44-45 0.10569816486911066 1101 46-47 0.13312351663653033 1101 48-49 0.02807410586789416 1101 50-51 0.08557120889017966 1101 52-53 0.20155644349912194 1101 54-55 0.18589880025926675 1101 56-57 0.20830386246136356 1101 58-59 0.2493774251332752 1101 60-61 0.3782387328901464 1101 62-63 0.22901886933866678 1101 64-65 0.24270252351799826 1101 66-67 0.21010099445834385 1101 68-69 0.2663803128690603 1101 70-71 0.2622907248366033 1101 72-73 0.2320429500149217 1101 74-75 0.2259962924390777 1101 76-77 0.13102947816088317 1101 78-79 0.2682335417654187 1101 80-81 0.32916475532950784 1101 82-83 0.21633811478373843 1101 84-85 0.22823784810631054 1101 86-87 0.1868515407597897 1101 88-89 0.1472839162159545 1101 90-91 0.14960239180420842 1101 92-93 0.1062480735971505 1101 94-95 0.13141650255338533 1101 96-97 0.06733807407035641 1101 98-99 0.08634471847108927 1101 100-101 0.06369781882489889 1102 1 0.30346478101552066 1102 2 0.1336998816463435 1102 3 0.15717311046006444 1102 4 0.19007160411482715 1102 5 0.13946361320974177 1102 6 0.17310593536500818 1102 7 0.1971549397218766 1102 8 0.19242300182894923 1102 9 0.20172072159229515 1102 10-11 0.17557364128860797 1102 12-13 0.23097932141292432 1102 14-15 0.30563187576291284 1102 16-17 0.3292890313708483 1102 18-19 0.297073262944231 1102 20-21 0.2911607395345257 1102 22-23 0.2777552693686971 1102 24-25 0.24831159268057235 1102 26-27 0.24487909866396507 1102 28-29 0.28196639246122146 1102 30-31 0.21575823980062125 1102 32-33 0.2865782616792245 1102 34-35 0.2588905214082118 1102 36-37 0.23406266468748527 1102 38-39 0.3757601568019098 1102 40-41 0.3062802597609817 1102 42-43 0.29697243811909146 1102 44-45 0.2353564408317368 1102 46-47 0.23381412476337715 1102 48-49 0.23452054683948376 1102 50-51 0.13960418042409373 1102 52-53 0.2537043172092268 1102 54-55 0.27012622483838555 1102 56-57 0.2538504692544308 1102 58-59 0.3128383539186501 1102 60-61 0.3188686718544673 1102 62-63 0.3598123010953671 1102 64-65 0.3518956009496108 1102 66-67 0.2739199258483396 1102 68-69 0.2894770341649533 1102 70-71 0.2353366211160477 1102 72-73 0.24785072100382166 1102 74-75 0.1712582371817959 1102 76-77 0.053149226268224936 1102 78-79 0.13769222973338913 1102 80-81 0.311085191873012 1102 82-83 0.2662569564201007 1102 84-85 0.2219824282418088 1102 86-87 0.270744163178847 1102 88-89 0.1906360447888673 1102 90-91 0.26111156467997176 1102 92-93 0.23924295604803092 1102 94-95 0.2640958959802262 1102 96-97 0.24571845597188968 1102 98-99 0.14961646490800007 1102 100-101 0.179465939266791 1103 1 0.3063753784823149 1103 2 0.07737638976168171 1103 3 0.11904567470940464 1103 4 0.1609319633530859 1103 5 0.16518708061634868 1103 6 0.14318531136395762 1103 7 0.21590683709266045 1103 8 0.14843789954306175 1103 9 0.25622226994037334 1103 10-11 0.2506494396806076 1103 12-13 0.20329094646708512 1103 14-15 0.3025200415581395 1103 16-17 0.3324575017647362 1103 18-19 0.25372325749102487 1103 20-21 0.3097022238188316 1103 22-23 0.29015929616083014 1103 24-25 0.28613147463649113 1103 26-27 0.3114658979781595 1103 28-29 0.25484940594133576 1103 30-31 0.2283092050648179 1103 32-33 0.25558838851038956 1103 34-35 0.2704753399169917 1103 36-37 0.31042537289562233 1103 38-39 0.3084492442298341 1103 40-41 0.22923674832193797 1103 42-43 0.30300743612776415 1103 44-45 0.3023789121310969 1103 46-47 0.30688392661620156 1103 48-49 0.30862037919870744 1103 50-51 0.2503074733070676 1103 52-53 0.41504673399757763 1103 54-55 0.43669834199499036 1103 56-57 0.4475135050874286 1103 58-59 0.4234023065799164 1103 60-61 0.4319352543870778 1103 62-63 0.291597430524547 1103 64-65 0.33219433142225085 1103 66-67 0.35537159144910646 1103 68-69 0.277568705535721 1103 70-71 0.3703813181159461 1103 72-73 0.4151536729474614 1103 74-75 0.30408027061151444 1103 76-77 0.2354317780786559 1103 78-79 0.2463314286860907 1103 80-81 0.26837513444310446 1103 82-83 0.18194945920310346 1103 84-85 0.3510520949626752 1103 86-87 0.2875316205950398 1103 88-89 0.25803498016878734 1103 90-91 0.24767101964462057 1103 92-93 0.2706687349943522 1103 94-95 0.2540263439344912 1103 96-97 0.2478621561865424 1103 98-99 0.23381735282784177 1103 100-101 0.19301174929346843 1104 1 0.21680912839010347 1104 2 0.04925859366028362 1104 3 0.06432780922557413 1104 4 0.10224246294801986 1104 5 0.13785109624272707 1104 6 0.09599551817129282 1104 7 0.10708500296279055 1104 8 0.1142608386648476 1104 9 0.16640862524358369 1104 10-11 0.16117386362898856 1104 12-13 0.2002778816811599 1104 14-15 0.23879857967319396 1104 16-17 0.262999171691753 1104 18-19 0.13756583750554796 1104 20-21 0.1942240755349829 1104 22-23 0.16896924303591732 1104 24-25 0.21294806467239624 1104 26-27 0.13541015829459724 1104 28-29 0.10541231822136155 1104 30-31 0.10808740516719695 1104 32-33 0.07794730837882469 1104 34-35 0.1402253829311917 1104 36-37 0.13694550359609536 1104 38-39 0.15411939647378858 1104 40-41 0.07932384577537022 1104 42-43 0.19226902144358604 1104 44-45 0.024125356841146584 1104 46-47 0.13107821220383187 1104 48-49 0.18610161554932603 1104 50-51 0.19726696563095203 1104 52-53 0.25392909472868297 1104 54-55 0.2350740900698156 1104 56-57 0.1534940289751674 1104 58-59 0.08092018643725396 1104 60-61 0.19976101515067768 1104 62-63 0.2079014290146617 1104 64-65 0.17032879923335997 1104 66-67 0.19884715205255787 1104 68-69 0.172487343495213 1104 70-71 0.1748117412939365 1104 72-73 0.25213064745025804 1104 74-75 0.2106523138793861 1104 76-77 0.201947310150004 1104 78-79 0.23517393201948522 1104 80-81 0.2733204518397443 1104 82-83 0.17784433194432125 1104 84-85 0.23003208364969652 1104 86-87 0.21307485604854293 1104 88-89 0.2106947352346964 1104 90-91 0.21526999455780071 1104 92-93 0.2731231469412947 1104 94-95 0.26159628300489857 1104 96-97 0.1497826211719797 1104 98-99 0.10640694857498545 1104 100-101 0.22816170904427224 1105 1 0.3401207240343709 1105 2 0.16872648040453697 1105 3 0.18104243016580313 1105 4 0.1608854013331822 1105 5 0.1546727386850293 1105 6 0.1907232201832727 1105 7 0.20750041520784634 1105 8 0.17755523545052654 1105 9 0.2713938344000084 1105 10-11 0.2884509112190301 1105 12-13 0.23078856191762043 1105 14-15 0.35899505172205437 1105 16-17 0.32154821185084614 1105 18-19 0.3467302544231643 1105 20-21 0.39056795081716444 1105 22-23 0.33164267536944436 1105 24-25 0.3044479527312305 1105 26-27 0.3681455896789103 1105 28-29 0.2822891058931276 1105 30-31 0.30397733496953094 1105 32-33 0.39943760308565857 1105 34-35 0.41657260708361576 1105 36-37 0.3891945971098636 1105 38-39 0.40671124348560994 1105 40-41 0.4001532942568957 1105 42-43 0.41862105912874625 1105 44-45 0.44282603764074224 1105 46-47 0.4182591939172724 1105 48-49 0.356500486972557 1105 50-51 0.35267464812908855 1105 52-53 0.43057350926387983 1105 54-55 0.351687383336575 1105 56-57 0.3479888459054692 1105 58-59 0.41512345187292965 1105 60-61 0.40528200608662246 1105 62-63 0.4830214208335306 1105 64-65 0.47452096794649634 1105 66-67 0.3448523381814397 1105 68-69 0.2589433235198655 1105 70-71 0.2596778195733407 1105 72-73 0.3064784287487967 1105 74-75 0.2968496991883782 1105 76-77 0.2805675331557964 1105 78-79 0.3298157271437887 1105 80-81 0.32626850027374843 1105 82-83 0.2840800606938316 1105 84-85 0.33826826915413477 1105 86-87 0.2861569566867175 1105 88-89 0.33053446894332694 1105 90-91 0.23754129275446445 1105 92-93 0.266531603850062 1105 94-95 0.27251082800244575 1105 96-97 0.2543176932207629 1105 98-99 0.2982181117504936 1105 100-101 0.3154093310279933 1106 1 0.19037671640654708 1106 2 0.029709465868855034 1106 3 0.07287096722255626 1106 4 0.11958982165585752 1106 5 0.06803415204644381 1106 6 0.07307584008776047 1106 7 0.12044067669582859 1106 8 0.09982552805276868 1106 9 0.1248858995103248 1106 10-11 0.15529992329625486 1106 12-13 0.1275867517655982 1106 14-15 0.12940890201142707 1106 16-17 0.16814928563034215 1106 18-19 0.13655010337164697 1106 20-21 0.138926793574349 1106 22-23 0.14474882457518135 1106 24-25 0.1767331009499813 1106 26-27 0.08793728820504754 1106 28-29 0.1292015529093078 1106 30-31 0.1621558373389007 1106 32-33 0.14806491499097518 1106 34-35 0.21206884293254546 1106 36-37 0.1708310525015051 1106 38-39 0.1741270264615551 1106 40-41 0.14388031599222018 1106 42-43 0.2125722016276015 1106 44-45 0.17489941079295335 1106 46-47 0.1526802156827003 1106 48-49 0.21856293202255728 1106 50-51 0.1932192059972877 1106 52-53 0.29373331588842433 1106 54-55 0.33263153544255175 1106 56-57 0.2577126965006826 1106 58-59 0.3025829933781168 1106 60-61 0.36880102977162466 1106 62-63 0.42000502431090325 1106 64-65 0.43870946533001387 1106 66-67 0.45486327599321186 1106 68-69 0.3698051216223277 1106 70-71 0.3794347214879181 1106 72-73 0.34758169858651655 1106 74-75 0.34582674920277867 1106 76-77 0.28886926189320405 1106 78-79 0.2834296107369241 1106 80-81 0.26522253621948977 1106 82-83 0.16838496833898375 1106 84-85 0.246587208074736 1106 86-87 0.24759176407587802 1106 88-89 0.23214289285465384 1106 90-91 0.2057635026779181 1106 92-93 0.19493238702263227 1106 94-95 0.21827277791376787 1106 96-97 0.28989562039620154 1106 98-99 0.18496946271346815 1106 100-101 0.22643553614133083 1107 1 0.14613604716587503 1107 2 0.07356988472927384 1107 3 0.06137570572729345 1107 4 0.09165604372208236 1107 5 0.0871051711174573 1107 6 0.057456324468248 1107 7 0.11403492029006657 1107 8 0.0702244984517435 1107 9 0.037238186862616374 1107 10-11 0.09983455533089369 1107 12-13 0.11097273515158435 1107 14-15 0.13780366040618475 1107 16-17 0.16322065570171418 1107 18-19 0.16893719825873177 1107 20-21 0.19801185265940546 1107 22-23 0.1995048793312364 1107 24-25 0.1399804391973234 1107 26-27 0.16804580581356987 1107 28-29 0.10021477392253075 1107 30-31 0.0916632668463393 1107 32-33 0.14813072755679002 1107 34-35 0.06401250737621211 1107 36-37 0.07838633505678416 1107 38-39 0.17679609163062082 1107 40-41 0.12829005040195085 1107 42-43 -0.04631249475709609 1107 44-45 0.015069938463483368 1107 46-47 0.04917167467415595 1107 48-49 0.09430149526112075 1107 50-51 0.08201059478867734 1107 52-53 0.07432153397663654 1107 54-55 0.11254700285802244 1107 56-57 0.12820819199618683 1107 58-59 0.16342597922110258 1107 60-61 0.23982302579361914 1107 62-63 0.20657487338075242 1107 64-65 0.17341901253956138 1107 66-67 0.16824955187948376 1107 68-69 0.06763038194758764 1107 70-71 0.15416562121881583 1107 72-73 0.14891549992031372 1107 74-75 0.19861866449468835 1107 76-77 0.10725583027977592 1107 78-79 0.15996523727255152 1107 80-81 0.12869071218765527 1107 82-83 0.17116372111773615 1107 84-85 0.14031453930206084 1107 86-87 0.13244902393313396 1107 88-89 0.11422139993315739 1107 90-91 0.16328514769956115 1107 92-93 0.07446614155638542 1107 94-95 0.07353632067731297 1107 96-97 0.15021941821999718 1107 98-99 0.10464888239288683 1107 100-101 0.09582684303263989 1108 1 0.12273638450172797 1108 2 0.010246487713452268 1108 3 0.029356363678232356 1108 4 0.09728597461204203 1108 5 0.08172933736123866 1108 6 0.015786402486291706 1108 7 0.07763459317651922 1108 8 0.048709298258977185 1108 9 0.06633824125984944 1108 10-11 0.09746996427759314 1108 12-13 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66-67 -0.5540938142138785 2316 68-69 -0.5110090529418514 2316 70-71 -0.5128184082652183 2316 72-73 -0.5081391683843535 2316 74-75 -0.5257271586011356 2316 76-77 -0.5380942925703422 2316 78-79 -0.5467577357004174 2316 80-81 -0.4533608848639332 2316 82-83 -0.4787287850247708 2316 84-85 -0.4870103992728758 2316 86-87 -0.5039637524796348 2316 88-89 -0.5046975189857648 2316 90-91 -0.5186620267476059 2316 92-93 -0.45179921470897 2316 94-95 -0.35995569406470196 2316 96-97 -0.5331620314114502 2316 98-99 -0.5716871126931053 2316 100-101 -0.43677757957178187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 2.0 11 1.0 12 3.0 13 14.0 14 70.0 15 261.0 16 812.0 17 1838.0 18 3431.0 19 5012.0 20 7356.0 21 10576.0 22 12982.0 23 12721.0 24 11619.0 25 11597.0 26 13510.0 27 16848.0 28 21121.0 29 27051.0 30 33383.0 31 41704.0 32 53108.0 33 69283.0 34 97855.0 35 147276.0 36 258669.0 37 631625.0 38 1246529.0 39 434963.0 40 131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66916857847717 20.74636619334507 12.800562157443194 22.78390307073457 2 13.870596988494333 21.944609028582505 42.894431893259835 21.290362089663333 3 17.210099285699197 30.287829831620762 30.276415145207743 22.2256557374723 4 10.179283100935153 17.65886673605871 43.21417388729669 28.947676275709455 5 10.981022926176935 41.94304449867297 35.7911591677117 11.284773407438403 6 26.887749760661144 40.33976027012812 21.089884196571937 11.682605772638807 7 26.25635681363051 33.67967052914491 24.652182977822793 15.411789679401794 8 25.056529500184936 36.56707405325108 22.120211783424928 16.256184663139045 9 28.173432601164233 15.165672191017524 21.221604785086996 35.43929042273125 10-11 23.364712159132083 27.77090724369063 30.775350457675323 18.089030139501972 12-13 24.235239659539634 25.676596077447073 30.126731398239176 19.96143286477412 14-15 22.05916528371329 26.548999748687706 27.950499360998286 23.441335606600717 16-17 20.792072027301913 29.663112242629563 28.243576164495092 21.301239565573432 18-19 20.53021218388492 29.04197356774853 30.18352103975811 20.24429320860844 20-21 22.299267851923137 28.20181165578225 29.5010363084778 19.997884183816815 22-23 21.578282203211057 28.443837693249534 28.910531246442766 21.06734885709664 24-25 20.94530315609773 28.336848657339626 29.68870069021014 21.029147496352504 26-27 20.95536195434567 28.436254179209946 29.40536736213219 21.203016504312195 28-29 21.00581449156269 28.01997161732229 29.86216537978662 21.112048511328396 30-31 21.523699821495747 28.543227448978403 29.173053267800842 20.76001946172501 32-33 21.073071966444605 28.60898802498492 29.323005039174134 20.994934969396343 34-35 21.059654980066867 28.56723928241353 29.823974183889334 20.549131553630264 36-37 21.385782080186672 28.09186939316064 29.11279108266196 21.409557443990728 38-39 20.888702708276245 28.938326953826966 29.576067375659537 20.596902962237255 40-41 21.102743844661884 28.23167272769698 29.885414837137336 20.7801685905038 42-43 20.985301856967673 28.059396100024188 29.342208199465652 21.61309384354249 44-45 21.37546655954099 28.343817291862493 29.18017956373825 21.10053658485826 46-47 21.18220519759232 28.155522264471976 29.038173927658008 21.624098610277695 48-49 20.729259719747375 28.37046207092052 29.339023438891854 21.56125477044025 50-51 21.21091534118088 28.13042256727649 28.57854360583637 22.080118485706258 52-53 21.249510855461377 28.283842889769762 28.928898801237203 21.537747453531665 54-55 21.272135268448515 28.32191812138226 28.927148759535758 21.47879785063347 56-57 21.443465927634044 28.551189350412898 28.352173346833354 21.653171375119705 58-59 21.24526976341013 28.360434804955485 28.95273720711633 21.441558224518054 60-61 21.590176681743305 28.888688483649876 28.426756159518085 21.09437867508873 62-63 21.108086278314715 28.69514367973301 29.14905253817667 21.04771750377561 64-65 21.077336394079474 28.438593780547023 28.921701867349448 21.562367958024055 66-67 21.185106167619942 28.825157590466908 28.352078749984628 21.637657491928525 68-69 21.401859080335743 28.79849704526743 28.27810401428034 21.521539860116487 70-71 21.20730489478781 28.104487264583916 29.008707009279632 21.679500831348637 72-73 21.217379459177202 28.522163883995255 28.574397110633853 21.68605954619369 74-75 21.160290260970633 28.398746528689806 28.383942121864074 22.057021088475487 76-77 20.6455888070486 28.60142027708678 29.272553386519885 21.48043752934473 78-79 20.952823605571503 28.75777310189058 28.47347803918391 21.81592525335401 80-81 21.015273291872585 28.778505577903186 28.13406454595247 22.07215658427176 82-83 21.37791031146643 28.495960556897952 28.567885694213164 21.55824343742245 84-85 21.331857412277976 28.30001895090203 28.440999787787735 21.92712384903226 86-87 20.432257147028412 28.76212455693201 29.08337545520792 21.722242840831658 88-89 21.296430892429825 28.19309297955794 28.591645269385026 21.918830858627217 90-91 20.961494983371452 28.526341911480685 29.063998867360397 21.448164237787466 92-93 21.47930236716001 28.55916701798885 28.667748434185665 21.293782180665477 94-95 21.09355218419394 28.099915083562127 28.769771135537418 22.036761596706516 96-97 21.295752948347282 28.141348503304425 28.850493779784216 21.71240476856408 98-99 20.992167529942694 28.25376074128695 28.84090826318575 21.913163465584603 100-101 20.791144159975733 28.386645859419907 28.939126820883725 21.883083159720634 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 19.0 1 21.5 2 68.0 3 130.5 4 107.5 5 76.0 6 141.5 7 259.0 8 421.5 9 533.0 10 509.0 11 505.5 12 575.0 13 652.5 14 726.5 15 820.0 16 934.0 17 1081.0 18 1241.5 19 1411.0 20 1588.0 21 1737.5 22 1985.0 23 2372.5 24 2775.0 25 3338.5 26 4802.0 27 7480.5 28 11406.0 29 19607.5 30 31507.0 31 44015.5 32 66475.5 33 93263.0 34 116106.0 35 135826.0 36 161474.5 37 180135.5 38 169673.5 39 160390.5 40 148453.5 41 130643.0 42 121198.5 43 115477.0 44 114378.5 45 117452.5 46 117862.0 47 111179.0 48 106843.5 49 104653.5 50 102774.0 51 96654.0 52 85862.0 53 77653.0 54 70669.0 55 62490.5 56 55529.5 57 48450.0 58 40591.5 59 32958.5 60 25229.0 61 17799.5 62 12665.5 63 9592.5 64 6380.0 65 3643.5 66 2225.0 67 1580.0 68 1280.0 69 913.5 70 588.0 71 450.5 72 307.0 73 229.0 74 175.0 75 107.5 76 75.5 77 54.0 78 37.5 79 26.0 80 20.0 81 11.5 82 5.0 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022419453148230423 2 0.1083449240749926 3 0.0 4 0.0 5 0.0 6 0.0036892771003417153 7 0.029514216802733723 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0016554448527174364 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.459684872671066E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 3.153228290890355E-5 62-63 3.6262125345239084E-4 64-65 4.0991967781574615E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.1036299018116243E-4 100-101 6.937102239958781E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3171353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.4818623194008 #Duplication Level Percentage of deduplicated Percentage of total 1 54.48522061271493 11.70444007644521 2 15.120080092561375 6.496149576134328 3 7.871784993697707 5.073018043276183 4 4.6629074991835795 4.0067174762225255 5 3.328243116830882 3.57484302006272 6 2.3122939774349933 2.9803428519142914 7 1.7491928445594342 2.630314389983477 8 1.3478135630350947 2.316283631467275 9 1.0939420251485246 2.1149920772682385 >10 7.166920266342484 28.799501421092515 >50 0.5135935661905128 7.583265920702605 >100 0.30257156636211235 12.342919358455202 >500 0.030041483761949383 4.32485243903771 >1k 0.014796551703646148 5.340455268629102 >5k 5.978404728745918E-4 0.7119044493086986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 5913 0.18645038884034668 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 5593 0.17636005830949755 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 5520 0.1740582016571476 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5147 0.16229666013212657 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3831 0.12080017582400951 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3693 0.11644872078258081 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3667 0.11562888142694931 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3345 0.10547548633028238 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 3271 0.1031420973950235 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3231 0.10188080607866735 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 3190 0.10058798247940233 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.153228290890355E-5 0.0 10-11 0.0 0.0 0.0 6.30645658178071E-5 0.0 12-13 0.0 0.0 0.0 6.30645658178071E-5 0.0 14-15 0.0 0.0 0.0 6.30645658178071E-5 0.0 16-17 0.0 0.0 0.0 1.8919369745342129E-4 0.0 18-19 0.0 0.0 0.0 3.7838739490684257E-4 0.0 20-21 0.0 0.0 0.0 5.675810923602639E-4 0.0 22-23 0.0 1.5766141454451774E-5 0.0 0.0010563314774482688 0.0 24-25 0.0 3.153228290890355E-5 0.0 0.0015608480039907258 0.0 26-27 0.0 3.153228290890355E-5 0.0 0.0018288724087164058 0.0 28-29 0.0 3.153228290890355E-5 0.0 0.002175727520714345 0.0 30-31 0.0 3.153228290890355E-5 0.0 0.002979800734891385 0.0 32-33 0.0 3.153228290890355E-5 0.0 0.004556414880336563 0.0 34-35 0.0 3.153228290890355E-5 0.0 0.006937102239958781 0.0 36-37 0.0 3.153228290890355E-5 0.0 0.010894403745026177 0.0 38-39 0.0 3.153228290890355E-5 0.0 0.019597313827883555 0.0 40-41 0.0 3.153228290890355E-5 0.0 0.032304823840171686 0.0 42-43 0.0 3.153228290890355E-5 0.0 0.04526459211573104 0.0 44-45 0.0 3.153228290890355E-5 0.0 0.06071541074109379 0.0 46-47 0.0 3.153228290890355E-5 0.0 0.07949288521334585 0.0 48-49 0.0 3.153228290890355E-5 0.0 0.10238532260520983 0.0 50-51 0.0 3.153228290890355E-5 0.0 0.12862018198541758 0.0 52-53 0.0 3.153228290890355E-5 0.0 0.15808710036378795 0.0 54-55 0.0 3.153228290890355E-5 0.0 0.18668688096216346 0.0 56-57 0.0 3.153228290890355E-5 0.0 0.2234219905510361 0.0 58-59 0.0 3.153228290890355E-5 0.0 0.26747258977477434 0.0 60-61 0.0 3.153228290890355E-5 0.0 0.31794000857047444 0.0 62-63 0.0 3.153228290890355E-5 0.0 0.374177835138504 0.0 64-65 0.0 3.153228290890355E-5 0.0 0.4342468340799652 0.0 66-67 0.0 3.153228290890355E-5 0.0 0.49969208725739456 0.0 68-69 0.0 3.153228290890355E-5 0.0 0.5802728362310976 0.0 70-71 0.0 3.153228290890355E-5 0.0 0.6856537257126533 0.0 72-73 0.0 3.153228290890355E-5 0.0 0.7986969599410725 0.0 74-75 0.0 3.153228290890355E-5 0.0 0.9103527737214999 0.0 76-77 0.0 3.153228290890355E-5 0.0 1.0354255738796658 0.0 78-79 0.0 3.153228290890355E-5 0.0 1.1849201271507774 0.0 80-81 0.0 3.153228290890355E-5 0.0 1.3663884152915173 0.0 82-83 0.0 3.153228290890355E-5 0.0 1.57035498728776 0.0 84-85 0.0 3.153228290890355E-5 0.0 1.7784995867694326 0.0 86-87 0.0 3.153228290890355E-5 0.0 2.001543189925562 0.0 88-89 0.0 3.153228290890355E-5 0.0 2.2541640744502427 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 52.855057 1 GTATCAA 8220 0.0 43.644436 1 GTACATG 22565 0.0 37.280037 1 ACATGGG 22735 0.0 35.29776 3 GCGCGTA 55 7.020935E-8 34.54565 24-25 TACATGG 24420 0.0 34.10561 2 TCAACGC 10710 0.0 33.13932 4 CAACGCA 10840 0.0 32.785725 5 ATCAACG 10950 0.0 32.629936 3 CATGGGG 9975 0.0 32.008762 4 CATGGGA 11880 0.0 31.83531 4 AACGCAG 11370 0.0 31.633547 6 GACATAT 1205 0.0 30.799023 1 ATGGGGT 3585 0.0 30.084942 5 TATCAAC 12010 0.0 29.822037 2 ATGGGAT 5205 0.0 28.845552 5 ACGCAGA 13515 0.0 26.471449 7 CGCAGAG 13620 0.0 26.050266 8 GTGTAGC 2710 0.0 24.40478 1 ATGGGGG 4005 0.0 23.964113 5 >>END_MODULE