FastQCFastQC Report
Fri 10 Feb 2017
SRR2568712_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568712_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332284
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC137790.5907942600472327No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC99730.42760658650490246No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA86190.369551907057631No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC75580.3240600201347692No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT73500.31514172373518834No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG71420.3062234273356075No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA63270.27127914096224987No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT57610.24701108441339048No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT57380.24602492663843684No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA56090.24049386781369678No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG54770.2348341797139628No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA52510.22514410766441822No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT51100.21909853173970234No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT49420.21189529234004095No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT48090.20619272781530895No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG48020.20589259284032307No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC47910.20542095216534523No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT46380.19886085914065355No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC44550.1910144733660223No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA44430.1904999562660465No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC41400.1775083994916571No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC40640.17424979119181028No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA40270.17266336346688482No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT40200.17236322849189895No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC40100.17193446424191908No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG39040.1673895631921327No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC39030.1673466867671347No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT37630.16134398726741683No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA37220.15958605384249946No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT35910.15396924216776345No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA35700.15306883724280576No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA35580.15255432014282994No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC35560.15246856729283398No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT35220.1510107688429025No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA35190.15088213956790855No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG34940.14981022894295892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34060.14603710354313626No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC33910.1453939571681665No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT33240.1425212366933015No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG33010.14153507891834785No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA32490.13930550481845266No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT30560.13103035479384156No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC30400.1303443319938738No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC29970.12850064571896047No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA29940.1283720164439665No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT29820.12785749934399068No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA29680.1272572293940189No Hit
CCTACTACCTGTGCCTTTAAGTACCTTTTCTGTCTTCTAAAGATGCTTTG29540.12665695944404712No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG28950.12412725036916603No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA28790.12344122756919826No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA28750.12326972186920632No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG28590.12258369906923856No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT28480.12211205839426074No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC28030.12018261926935142No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA27860.11945372004438568No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT27800.11919646149439776No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC27690.11872482081941994No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC27440.1176529101944703No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC27420.11756715734447434No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC26510.11366540266965772No Hit
CCATAATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATAT25940.1112214464447726No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG25460.10916337804486931No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC25380.10882036664488545No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC25120.10770557959493784No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC25080.1075340738949459No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA25020.10727681534495799No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA24760.10616202829501038No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC24660.10573326404503054No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG24570.10534737622004868No Hit
CATAATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAG24470.10491861197006883No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA24290.1041468363201051No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT24280.10410395989510711No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC24000.10290341999516354No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT23890.10243177932018571No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG23840.10221739719519579No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG23700.101617127245224No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA23630.1013169922702381No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC558.198258E-751.8146743
ACATATA7950.044.812132
GGTATCA38450.043.9799841
GACATAT8200.043.4459081
CATTCCG5400.042.220279
TAAGGCG459.6293073E-442.220275
GTATCAA70950.041.7766531
TTCGATC704.3115742E-640.715022
ACCCTTG14400.040.241197
TATCACG608.2925E-539.584052
TAACTTT18300.039.4517256
CATATAG9350.038.6070143
TCACGCA756.9275466E-637.998244
CCTTGGC15750.037.093529
AACCCTT16750.034.8789866
AACTTTA22050.034.4655277
CTTAACT1255.660695E-934.1984184
CCCTTGG17150.034.065488
AACGCAG85400.033.7601476
TCAACGC85500.033.6095544
Produced by FastQC (version 0.11.5)