##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568712_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332284 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.211204124369075 34.0 31.0 34.0 30.0 34.0 2 32.35614059008251 34.0 31.0 34.0 31.0 34.0 3 32.376743569822544 34.0 31.0 34.0 30.0 34.0 4 35.74009768964672 37.0 35.0 37.0 35.0 37.0 5 35.803568519099734 37.0 35.0 37.0 35.0 37.0 6 35.92190745209417 37.0 37.0 37.0 35.0 37.0 7 35.90443273632199 37.0 37.0 37.0 35.0 37.0 8 35.91895326641181 37.0 37.0 37.0 35.0 37.0 9 37.73722068152935 39.0 39.0 39.0 35.0 39.0 10-11 37.703495157536565 39.0 38.5 39.0 35.0 39.0 12-13 37.71784289563364 39.0 39.0 39.0 35.0 39.0 14-15 39.12818250264547 41.0 39.0 41.0 36.0 41.0 16-17 39.11515964608084 41.0 39.0 41.0 36.0 41.0 18-19 39.16559861491997 41.0 39.5 41.0 36.0 41.0 20-21 39.19592661099591 41.0 39.0 41.0 36.0 41.0 22-23 39.11238940026172 41.0 39.0 41.0 36.0 41.0 24-25 39.04359996466982 41.0 39.0 41.0 36.0 41.0 26-27 38.97489328057818 41.0 39.0 41.0 36.0 41.0 28-29 38.87594070876446 41.0 39.0 41.0 35.0 41.0 30-31 38.78587749176344 40.5 39.0 41.0 35.0 41.0 32-33 38.68203786502845 40.0 38.5 41.0 35.0 41.0 34-35 38.59901002622322 40.0 38.0 41.0 35.0 41.0 36-37 38.506139260913336 40.0 38.0 41.0 34.5 41.0 38-39 38.383509040922974 40.0 38.0 41.0 34.0 41.0 40-41 38.31335806445527 40.0 38.0 41.0 34.0 41.0 42-43 38.19144538143725 40.0 38.0 41.0 34.0 41.0 44-45 38.03702936692101 40.0 38.0 41.0 33.0 41.0 46-47 38.09397762022121 40.0 38.0 41.0 33.5 41.0 48-49 38.26106211764948 40.0 38.0 41.0 34.0 41.0 50-51 37.19929069530126 39.0 37.0 40.0 32.5 40.5 52-53 37.36794082538833 39.5 37.5 40.0 33.0 40.5 54-55 38.03333084650068 40.0 38.0 41.0 33.0 41.0 56-57 38.05472168055005 40.0 38.0 41.0 33.0 41.0 58-59 37.97243281692967 40.0 38.0 41.0 33.0 41.0 60-61 37.808171732087516 40.0 38.0 41.0 33.0 41.0 62-63 37.56608178935327 40.0 37.5 41.0 32.5 41.0 64-65 37.444937237489086 40.0 37.0 41.0 32.5 41.0 66-67 37.358854453402756 39.0 37.0 41.0 32.0 41.0 68-69 37.065606932946416 39.0 36.0 41.0 32.0 41.0 70-71 36.672566033982136 39.0 36.0 41.0 32.0 41.0 72-73 36.32196593553787 37.5 35.0 40.0 31.0 41.0 74-75 35.91360657621456 37.0 35.0 39.5 31.0 41.0 76-77 35.51739110674343 37.0 35.0 39.0 31.0 41.0 78-79 35.10837509497128 36.0 35.0 39.0 30.5 40.5 80-81 34.66497047529375 36.0 35.0 37.5 30.0 39.0 82-83 34.28767572902785 35.0 35.0 37.0 30.0 39.0 84-85 33.87029259729947 35.0 34.0 36.5 29.0 38.5 86-87 33.59417635245107 35.0 34.0 36.0 29.0 37.0 88-89 33.34747697964742 35.0 34.0 36.0 29.0 37.0 90-91 33.1430599789734 35.0 34.0 36.0 29.0 37.0 92-93 32.939977292645324 35.0 34.0 35.0 29.0 36.0 94-95 32.752351128764765 35.0 34.0 35.0 28.0 36.0 96-97 32.61384612680102 35.0 34.0 35.0 27.0 36.0 98-99 32.45458250367451 35.0 34.0 35.0 26.5 36.0 100-101 31.136144654767598 34.5 31.5 35.0 22.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12507229592007718 1101 2 0.18178616939447068 1101 3 0.29179530987005364 1101 4 0.17591080852994168 1101 5 0.09953913819306592 1101 6 0.06131650299351321 1101 7 0.04798145824988609 1101 8 0.1671399827692781 1101 9 0.23418333356331544 1101 10-11 0.1778058362630901 1101 12-13 0.23102585847452417 1101 14-15 0.2777563522836104 1101 16-17 0.3151919170203641 1101 18-19 0.2877325645012405 1101 20-21 0.21022652747112858 1101 22-23 0.1314557718703142 1101 24-25 0.2040039374816658 1101 26-27 0.2567956650590375 1101 28-29 0.24501271435170224 1101 30-31 0.258765489458348 1101 32-33 0.19049378021811236 1101 34-35 0.06880335187577202 1101 36-37 0.0686484047867637 1101 38-39 0.07867564821845718 1101 40-41 0.1562822216501374 1101 42-43 0.2507160452962225 1101 44-45 0.2660399229678916 1101 46-47 0.2675265454864686 1101 48-49 0.2962251719458422 1101 50-51 0.04712222243680486 1101 52-53 0.0667322352909423 1101 54-55 0.17479612153067592 1101 56-57 0.18635173587114906 1101 58-59 0.19145514509464334 1101 60-61 0.15170433873106504 1101 62-63 0.17803349964034254 1101 64-65 0.14152248571883774 1101 66-67 0.0998472721971666 1101 68-69 0.16213443492730306 1101 70-71 0.10980257350058054 1101 72-73 0.06750185117503094 1101 74-75 0.038513641381960895 1101 76-77 0.11668701272832038 1101 78-79 0.11759668553533231 1101 80-81 0.04968954386011859 1101 82-83 0.028974183653119212 1101 84-85 0.006077297298290318 1101 86-87 -0.02473416458303035 1101 88-89 0.0034565007010058935 1101 90-91 0.03905348242937379 1101 92-93 -0.12823679944589372 1101 94-95 -0.19178313568377092 1101 96-97 -0.1571172654699282 1101 98-99 -0.03703852639902294 1101 100-101 -0.2806405102986034 1102 1 0.1986009187002722 1102 2 0.14632289346764793 1102 3 0.25252281671055243 1102 4 0.15541614679916194 1102 5 0.1392408389280817 1102 6 0.18932766239047538 1102 7 0.13999283225063408 1102 8 0.18586416325106825 1102 9 0.25800435400432775 1102 10-11 0.25770550899231637 1102 12-13 0.23184403541534948 1102 14-15 0.32851014808810675 1102 16-17 0.3766356640024995 1102 18-19 0.2700009265378256 1102 20-21 0.24623972560407736 1102 22-23 0.2551614427753961 1102 24-25 0.23675784058806215 1102 26-27 0.25235604919981824 1102 28-29 0.3755991192017518 1102 30-31 0.3680122301633233 1102 32-33 0.27829355488413654 1102 34-35 0.2586204021467111 1102 36-37 0.1870292192081493 1102 38-39 0.1358139067087265 1102 40-41 0.13207223184382144 1102 42-43 0.08433104610099207 1102 44-45 0.10924503053804102 1102 46-47 0.10318028227492704 1102 48-49 0.13882670636292715 1102 50-51 0.17371163173987014 1102 52-53 0.10424765993818852 1102 54-55 0.12993051715275783 1102 56-57 0.15201512983005472 1102 58-59 0.07468385908143915 1102 60-61 0.046494831783263635 1102 62-63 0.1329264660012015 1102 64-65 0.06493575896310944 1102 66-67 0.04056297582933155 1102 68-69 0.06927805851225344 1102 70-71 0.10088310357193109 1102 72-73 0.10712307548964617 1102 74-75 -0.004085210487779989 1102 76-77 0.005039392147821786 1102 78-79 -0.03681073975572957 1102 80-81 -0.010547056815873646 1102 82-83 -0.0756049246681414 1102 84-85 -0.03684346025308116 1102 86-87 -0.06393959405885852 1102 88-89 -0.0941748099916282 1102 90-91 -0.08694576645736873 1102 92-93 -0.03016286844101046 1102 94-95 -0.018262560545622364 1102 96-97 0.0013810445252389059 1102 98-99 0.06865383907122435 1102 100-101 -0.17897196584879183 1103 1 0.17932613013377363 1103 2 0.09309825817215511 1103 3 0.22501222674522836 1103 4 0.1874018658925607 1103 5 0.14952147300911633 1103 6 0.12382808672069245 1103 7 0.161032843663655 1103 8 0.2137442131464482 1103 9 0.22173372277654835 1103 10-11 0.228898036939583 1103 12-13 0.26651622660537555 1103 14-15 0.2826244136310265 1103 16-17 0.2571161768201975 1103 18-19 0.22582623051921757 1103 20-21 0.14381701954786053 1103 22-23 0.20436230277569223 1103 24-25 0.28762396806271084 1103 26-27 0.2650852698222721 1103 28-29 0.20679148253815072 1103 30-31 0.23785179401770762 1103 32-33 0.28538188141354937 1103 34-35 0.2698266293755367 1103 36-37 0.308983232420907 1103 38-39 0.24156312389263945 1103 40-41 0.34315742436536567 1103 42-43 0.29948932858786037 1103 44-45 0.3006463079183064 1103 46-47 0.301564603822122 1103 48-49 0.3991002530319392 1103 50-51 0.392381726884949 1103 52-53 0.31934364178709984 1103 54-55 0.4159258824425507 1103 56-57 0.4235061064114163 1103 58-59 0.3964501930512725 1103 60-61 0.3922462254885488 1103 62-63 0.3935646714501857 1103 64-65 0.2562028327673005 1103 66-67 0.2666869707885837 1103 68-69 0.3445354230192308 1103 70-71 0.32251310774207553 1103 72-73 0.26036801279119715 1103 74-75 0.297512749434965 1103 76-77 0.30672457296370226 1103 78-79 0.3394344728117602 1103 80-81 0.38047735314564335 1103 82-83 0.3130781244370979 1103 84-85 0.23239074716391883 1103 86-87 0.2555833453789802 1103 88-89 0.15485147104666908 1103 90-91 0.20118656787094125 1103 92-93 0.16099583654706606 1103 94-95 0.08703283364251746 1103 96-97 0.176468013929572 1103 98-99 0.2001375636297027 1103 100-101 0.16971670288831575 1104 1 0.0861511231029084 1104 2 0.054019389438416 1104 3 0.13162871060939096 1104 4 0.022345267425286863 1104 5 0.048967133207383995 1104 6 0.008826856319231524 1104 7 0.07952841421838741 1104 8 0.06543118143724058 1104 9 0.06622460022856558 1104 10-11 0.04182439301776242 1104 12-13 -0.025712140300342412 1104 14-15 0.027180326588911896 1104 16-17 0.07502609825026951 1104 18-19 0.08294366355023186 1104 20-21 0.06682025374599476 1104 22-23 0.08256596058347299 1104 24-25 0.03148997734345471 1104 26-27 0.11044292116164911 1104 28-29 0.10893820351825667 1104 30-31 0.14771906445995597 1104 32-33 0.08613154744743667 1104 34-35 0.13286152443987476 1104 36-37 0.06829363634413 1104 38-39 0.024923262400683655 1104 40-41 0.12261268608932596 1104 42-43 -0.0292923981927089 1104 44-45 0.13899001177825454 1104 46-47 0.07996106114476476 1104 48-49 0.28822526182052144 1104 50-51 0.16826003578602666 1104 52-53 0.18845056806846827 1104 54-55 0.19190624875064088 1104 56-57 0.2263592096596767 1104 58-59 0.14772354870759585 1104 60-61 0.17902907720759487 1104 62-63 0.20319190374998897 1104 64-65 0.2409394829114433 1104 66-67 0.17732609843394442 1104 68-69 0.16941227301571615 1104 70-71 0.17697880590701942 1104 72-73 0.21516877564010173 1104 74-75 0.16599447797324984 1104 76-77 0.16127123593916792 1104 78-79 0.15136662056539762 1104 80-81 0.09213936186391436 1104 82-83 0.11686842008499809 1104 84-85 0.1721917077559212 1104 86-87 0.06785176624658362 1104 88-89 0.09018662361644658 1104 90-91 0.08261431693934895 1104 92-93 0.08494757844398748 1104 94-95 0.0760416134358124 1104 96-97 0.008000127407740365 1104 98-99 0.039082955776223116 1104 100-101 0.12655301115660933 1105 1 0.15656285567444428 1105 2 0.09946485412645245 1105 3 0.25378630073102215 1105 4 0.13852293499999746 1105 5 0.023430248096119044 1105 6 0.04739814758099925 1105 7 0.08244379375184252 1105 8 0.10692831557609139 1105 9 0.18585663120006046 1105 10-11 0.13440478738849038 1105 12-13 0.20462291786246567 1105 14-15 0.2515005438371247 1105 16-17 0.2535106654267665 1105 18-19 0.24283146243989506 1105 20-21 0.3591222745096445 1105 22-23 0.24947247304154274 1105 24-25 0.22668073261436206 1105 26-27 0.27042817514715267 1105 28-29 0.3704008572898658 1105 30-31 0.36832442972189483 1105 32-33 0.2898917811450019 1105 34-35 0.2750492392577897 1105 36-37 0.4024107065592517 1105 38-39 0.28978905785205455 1105 40-41 0.37840274761622084 1105 42-43 0.2919046326510397 1105 44-45 0.4432995710159915 1105 46-47 0.3444226795320233 1105 48-49 0.3964088132010133 1105 50-51 0.4504158982392923 1105 52-53 0.4549918368340542 1105 54-55 0.4749285003891899 1105 56-57 0.41601315281938156 1105 58-59 0.4009649343949633 1105 60-61 0.5137692030353875 1105 62-63 0.4620446832885463 1105 64-65 0.44468183159141006 1105 66-67 0.34219092055102607 1105 68-69 0.42408969178183753 1105 70-71 0.45807750565407446 1105 72-73 0.3474936940929396 1105 74-75 0.3297377756541593 1105 76-77 0.44649906307986953 1105 78-79 0.40366143865530546 1105 80-81 0.36209362731894146 1105 82-83 0.26912795480687635 1105 84-85 0.1970366738534679 1105 86-87 0.3386142641630485 1105 88-89 0.27615868648897646 1105 90-91 0.29027397113800646 1105 92-93 0.3369536812499945 1105 94-95 0.28533707757635085 1105 96-97 0.3041420736139884 1105 98-99 0.2504710886412198 1105 100-101 0.14524309786852996 1106 1 0.2665757842472942 1106 2 0.2090811663182066 1106 3 0.34739150392684337 1106 4 0.2364131367301141 1106 5 0.2434834339323828 1106 6 0.17378843631912844 1106 7 0.17245469019128734 1106 8 0.20503504462380562 1106 9 0.23403757983263773 1106 10-11 0.16836442267893403 1106 12-13 0.21846595073981234 1106 14-15 0.20050801017553255 1106 16-17 0.23953449277484395 1106 18-19 0.29460660103956826 1106 20-21 0.2409638778862515 1106 22-23 0.30193666836927235 1106 24-25 0.28426929100258036 1106 26-27 0.3062898405855847 1106 28-29 0.3298792997413642 1106 30-31 0.29729249135859703 1106 32-33 0.25006687162748875 1106 34-35 0.2592630664073283 1106 36-37 0.2294447377863662 1106 38-39 0.2768380614643391 1106 40-41 0.2758146972613602 1106 42-43 0.2657215280884273 1106 44-45 0.3502540220304766 1106 46-47 0.2329739436007472 1106 48-49 0.23017773160384536 1106 50-51 0.1836321697883747 1106 52-53 0.19493365241677907 1106 54-55 0.3520486637073077 1106 56-57 0.23452016543146215 1106 58-59 0.22393983754745506 1106 60-61 0.3018154702749456 1106 62-63 0.3671370460874286 1106 64-65 0.2572314232377124 1106 66-67 0.2602419878298221 1106 68-69 0.2320329975957307 1106 70-71 0.319562738234076 1106 72-73 0.1384248900268048 1106 74-75 0.18712160244164266 1106 76-77 0.22327703381948538 1106 78-79 0.2288158632754147 1106 80-81 0.2535441851629159 1106 82-83 0.2457797931284773 1106 84-85 0.19884387110938917 1106 86-87 0.26815716261593536 1106 88-89 0.12245477299713059 1106 90-91 0.07510187630044385 1106 92-93 0.036185441392852624 1106 94-95 0.2246435594979559 1106 96-97 0.14219108540152803 1106 98-99 -0.04817824338250887 1106 100-101 -0.12509721440263633 1107 1 0.08728697808250985 1107 2 0.028117947483124794 1107 3 0.10202024926188358 1107 4 0.04794920959070481 1107 5 0.16715449552577155 1107 6 0.09926112391657682 1107 7 0.1208963268527512 1107 8 0.0716229691138679 1107 9 0.10684563592658236 1107 10-11 0.10491780443339138 1107 12-13 0.0842798064859025 1107 14-15 0.12264237972839709 1107 16-17 0.12224539390681599 1107 18-19 0.1174337016075242 1107 20-21 0.0382191282507236 1107 22-23 0.10332653971118333 1107 24-25 0.18487017699278852 1107 26-27 0.14303028973495913 1107 28-29 0.2991296041715046 1107 30-31 0.20825460317499278 1107 32-33 0.02066595636696178 1107 34-35 0.03785754669958408 1107 36-37 0.020389231118926432 1107 38-39 0.13841044078522913 1107 40-41 0.08852130165978878 1107 42-43 0.16600888387110757 1107 44-45 0.23399144845595288 1107 46-47 0.16502166548763597 1107 48-49 0.10064284831926074 1107 50-51 0.03703653369355919 1107 52-53 -0.01912482819069794 1107 54-55 0.14794303160902444 1107 56-57 0.1478834758301204 1107 58-59 0.1461117331574755 1107 60-61 0.20797246428766414 1107 62-63 0.12183343214049813 1107 64-65 0.20592129476725063 1107 66-67 0.22469580544942858 1107 68-69 0.2079561855093388 1107 70-71 0.25743187736755857 1107 72-73 0.21025201498669333 1107 74-75 0.24187090023530544 1107 76-77 0.2040726988093553 1107 78-79 0.1685425422381286 1107 80-81 0.1633037744777468 1107 82-83 0.26102231465435466 1107 84-85 0.312003415017692 1107 86-87 0.330370017917339 1107 88-89 0.2193886126820601 1107 90-91 0.18849501991454787 1107 92-93 0.2168714364806874 1107 94-95 0.17080614489318435 1107 96-97 0.26320070520628036 1107 98-99 0.31589703013553105 1107 100-101 0.3626777343273915 1108 1 0.06210480978745636 1108 2 0.09363938371328118 1108 3 0.1933563825893856 1108 4 0.22176333775403378 1108 5 0.13105556391820983 1108 6 0.00396877936138651 1108 7 0.10543219431300344 1108 8 0.004193347345577081 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-0.4809117875822082 2316 74-75 -0.26594992829015496 2316 76-77 -0.5195233176725154 2316 78-79 -0.40396478935971913 2316 80-81 -0.34796225621813903 2316 82-83 -0.2970014928255722 2316 84-85 -0.42261495260629545 2316 86-87 -0.44955003822004613 2316 88-89 -0.2784903895019468 2316 90-91 -0.3112564344269231 2316 92-93 -0.2578318059920761 2316 94-95 -0.2656909384384747 2316 96-97 -0.3503253940842157 2316 98-99 -0.28958377595093765 2316 100-101 -0.08948259159682337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 47.0 7 824.0 8 2306.0 9 2182.0 10 2346.0 11 2645.0 12 3128.0 13 3135.0 14 2936.0 15 3105.0 16 3709.0 17 4620.0 18 5465.0 19 6800.0 20 8408.0 21 9837.0 22 9779.0 23 9710.0 24 9640.0 25 9764.0 26 10901.0 27 12502.0 28 14871.0 29 18009.0 30 21797.0 31 27796.0 32 36106.0 33 49159.0 34 71050.0 35 105148.0 36 181611.0 37 446789.0 38 893227.0 39 342901.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.34163130210618 25.402324134474092 14.161603713675483 23.094440849744245 2 11.74626261517457 24.290814506335888 44.04231014633945 19.920612732150087 3 14.121709780199662 34.54680945849356 31.39650801178088 19.934972749525887 4 8.896499000761917 20.603041576965346 47.14349967864023 23.356959743632512 5 9.742525283752153 42.6345354594253 37.03354173196543 10.589397524857121 6 21.16250839879066 46.50878298039475 22.151239547873857 10.177469072940736 7 23.656734657124847 33.49053893550697 29.487863338750277 13.364863068617908 8 22.14918684179722 38.41620990968625 24.968152429269665 14.466450819246868 9 26.801497009582715 16.34472727771689 23.557576038241663 33.296199674458734 10-11 20.135789825067903 29.709770045325453 33.586627866715176 16.56781226289147 12-13 21.696822014912094 28.643757549199233 31.592838696150118 18.066581739738556 14-15 20.613377273093672 29.078641366145803 28.999191350624535 21.308790010135986 16-17 18.535264144503845 32.47166725836133 29.790261391837358 19.202807205297468 18-19 18.454668977998754 31.51623928032562 31.351378931404806 18.677712810270823 20-21 19.700268945431628 31.176596066257694 31.00013613419616 18.12299885411452 22-23 19.921861852320873 30.824264415993024 29.9238577537555 19.330015977930604 24-25 19.528920244278186 31.89223409578439 29.26553182986967 19.313313830067756 26-27 18.882625152350293 31.78561513072915 30.657177802575458 18.6745819143451 28-29 19.044360361774157 29.58983221412482 32.254622563783954 19.111184860317074 30-31 19.854106526291957 30.946264307191463 30.05989595393569 19.139733212580897 32-33 19.000755914937915 30.871750434018658 31.315716470584704 18.81177718045872 34-35 19.07214130011611 31.528793234443146 30.674651972058292 18.724413493382453 36-37 19.92090584184984 30.11079056212506 29.854582429623157 20.11372116640194 38-39 18.81631380767849 31.669519380589485 30.85471653942362 18.65945027230841 40-41 18.359706622349595 30.710325157656616 32.30459926835668 18.625368951637107 42-43 18.776651769224056 30.287279033237652 30.51782601527819 20.418243182260102 44-45 19.121311750945004 30.6468542262652 30.419114923130753 19.812719099659045 46-47 19.211626121083203 29.852703269959108 30.950343255020623 19.98532735393707 48-49 18.25452259701325 30.558031723245488 30.70469374200605 20.48275193773521 50-51 18.71228231230315 30.396364137643605 29.75553460738874 21.135818942664507 52-53 18.607167909225463 29.50376112000082 31.472432173783293 20.41663879699042 54-55 19.26491370690705 30.281389401976778 30.329904076861997 20.12379281425418 56-57 19.360742516777545 30.085851465773466 29.98404139461575 20.569364622833238 58-59 18.417311099334384 29.19395751117788 30.921898876809173 21.466832512678558 60-61 19.831589977892918 31.098999950263345 29.682362868329932 19.38704720351381 62-63 19.07718363606155 30.048804540655265 31.419726553078824 19.45428527020436 64-65 19.066161457212267 30.06460758225804 30.684678295574113 20.184552664955575 66-67 19.809096381822673 30.177847410883345 29.98586327899893 20.02719292829505 68-69 19.314832566249795 29.98898204044721 30.56682996001675 20.129355433286243 70-71 19.334893470875837 29.16678279086539 31.394931039376605 20.103392698882164 72-73 19.385267831876394 30.034785643600863 30.021129502239006 20.558817022283733 74-75 18.965679136846113 30.266275462164987 29.525842478874786 21.242202922114117 76-77 18.256653134866937 30.26192350502769 31.67148597683644 19.809937383268934 78-79 18.788149299141956 29.279603170111358 30.28683470795152 21.645412822795166 80-81 18.647050297517456 30.681369866773245 29.775223802768824 20.896356032940474 82-83 19.40095110268736 30.087498874371256 29.93535674926995 20.576193273671436 84-85 19.445342626010348 29.375325918403757 29.638740891438296 21.540590564147603 86-87 17.652324619267517 30.504376330315004 31.705211093264584 20.138087957152894 88-89 19.053161111156374 28.914933388337396 30.978293264561103 21.053612235945128 90-91 19.111710989277235 29.364974881590765 31.04011441044102 20.48319971869097 92-93 19.88799163657165 29.735902794870455 30.326304062763448 20.049801505794456 94-95 19.276036534830638 28.043602491264597 30.433441013660357 22.24691996024441 96-97 19.82012017716548 28.127978861580143 31.016909106424073 21.034991854830306 98-99 19.31051118272515 28.480067068477172 30.813934290903088 21.39548745789459 100-101 19.29531604385878 28.50220353105291 31.435470653391878 20.76700977169643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 114.0 1 85.5 2 102.0 3 148.0 4 123.5 5 127.0 6 213.5 7 382.0 8 602.5 9 703.5 10 618.5 11 527.0 12 533.0 13 575.5 14 592.5 15 622.0 16 677.5 17 718.5 18 753.5 19 832.5 20 946.5 21 1084.0 22 1229.5 23 1385.5 24 1606.0 25 1883.0 26 2416.5 27 3307.5 28 4383.0 29 6298.0 30 11130.0 31 18561.0 32 39630.5 33 69846.0 34 102872.5 35 144276.5 36 209239.0 37 268469.0 38 259023.5 39 229074.0 40 186258.0 41 133996.0 42 104917.5 43 87804.5 44 76583.5 45 61669.5 46 48230.5 47 37615.0 48 33701.5 49 30554.5 50 25505.0 51 19447.5 52 14908.5 53 12710.5 54 11205.0 55 9999.0 56 8717.0 57 7546.5 58 6638.5 59 5987.0 60 5294.5 61 4198.5 62 3000.5 63 2234.5 64 1651.0 65 1287.5 66 1002.0 67 739.5 68 575.0 69 460.0 70 415.5 71 338.5 72 254.0 73 201.0 74 185.5 75 163.0 76 117.0 77 92.5 78 70.0 79 54.5 80 50.5 81 55.0 82 49.5 83 36.5 84 26.5 85 18.0 86 12.0 87 10.0 88 11.0 89 8.5 90 6.0 91 4.5 92 3.5 93 2.5 94 1.0 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006302834474703767 2 0.007374745099653387 3 0.0013291691749375291 4 3.0013497498589364E-4 5 0.0023582033748891645 6 0.004416271774792435 7 0.004030383949810572 8 0.0034729904248367695 9 0.007203239399661447 10-11 0.006452901962196714 12-13 0.003558743274832739 14-15 0.0 16-17 0.0 18-19 3.0013497498589364E-4 20-21 0.0011362252624465973 22-23 1.9294391249093164E-4 24-25 0.0010719106249496202 26-27 0.0032800465123458376 28-29 0.002379641587388157 30-31 0.010290341999516353 32-33 4.7164067497783285E-4 34-35 0.0 36-37 2.1438212498992405E-5 38-39 8.575284999596962E-5 40-41 0.0 42-43 1.9294391249093164E-4 44-45 5.145170999758177E-4 46-47 0.0022724505248931945 48-49 0.002186697674897225 50-51 0.0031085408123538985 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 9.218431374566734E-4 64-65 0.0021438212498992404 66-67 0.0025725854998790883 68-69 0.007824947562132228 70-71 4.7164067497783285E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.013720455999355138 82-83 0.010890611949488142 84-85 0.01766508709916974 86-87 0.015628456911765464 88-89 0.014363602374324911 90-91 0.014106343824337 92-93 0.009690072049544567 94-95 0.02300320201141885 96-97 0.009732948474542551 98-99 0.01453510807431685 100-101 0.014792366624304757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2332284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.811643206892274 #Duplication Level Percentage of deduplicated Percentage of total 1 68.58543258805375 8.101066589204532 2 11.477048935548138 2.7112561418947476 3 4.933388169196524 1.748142625669585 4 2.8057945778493107 1.3256417786155594 5 1.839319581372337 1.0862693319310253 6 1.302789688447268 0.9232852184134486 7 0.9686984787892983 0.8009344564562945 8 0.7479735952710356 0.7067837788414335 9 0.5987818534512742 0.6365337850555297 >10 4.759676412001362 11.614754073525052 >50 0.8079310085592505 6.707145822001579 >100 0.9052269233246711 23.03865661408447 >500 0.1481892772371074 12.084371554825992 >1k 0.11488411139341406 24.37379146311304 >5k 0.004490584158700224 3.5323214414399597 >10k+ 3.7421534655835196E-4 0.6090453249277509 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 13779 0.5907942600472327 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 9973 0.42760658650490246 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 8619 0.369551907057631 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 7558 0.3240600201347692 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 7350 0.31514172373518834 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 7142 0.3062234273356075 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 6327 0.27127914096224987 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 5761 0.24701108441339048 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 5738 0.24602492663843684 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 5609 0.24049386781369678 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 5477 0.2348341797139628 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 5251 0.22514410766441822 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 5110 0.21909853173970234 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 4942 0.21189529234004095 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 4809 0.20619272781530895 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 4802 0.20589259284032307 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 4791 0.20542095216534523 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 4638 0.19886085914065355 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 4455 0.1910144733660223 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 4443 0.1904999562660465 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 4140 0.1775083994916571 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 4064 0.17424979119181028 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 4027 0.17266336346688482 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 4020 0.17236322849189895 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 4010 0.17193446424191908 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 3904 0.1673895631921327 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 3903 0.1673466867671347 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 3763 0.16134398726741683 No Hit TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA 3722 0.15958605384249946 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 3591 0.15396924216776345 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 3570 0.15306883724280576 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 3558 0.15255432014282994 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 3556 0.15246856729283398 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 3522 0.1510107688429025 No Hit ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA 3519 0.15088213956790855 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 3494 0.14981022894295892 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3406 0.14603710354313626 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 3391 0.1453939571681665 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 3324 0.1425212366933015 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 3301 0.14153507891834785 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 3249 0.13930550481845266 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 3056 0.13103035479384156 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 3040 0.1303443319938738 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2997 0.12850064571896047 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 2994 0.1283720164439665 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2982 0.12785749934399068 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 2968 0.1272572293940189 No Hit CCTACTACCTGTGCCTTTAAGTACCTTTTCTGTCTTCTAAAGATGCTTTG 2954 0.12665695944404712 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 2895 0.12412725036916603 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2879 0.12344122756919826 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2875 0.12326972186920632 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 2859 0.12258369906923856 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2848 0.12211205839426074 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2803 0.12018261926935142 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2786 0.11945372004438568 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 2780 0.11919646149439776 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 2769 0.11872482081941994 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 2744 0.1176529101944703 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 2742 0.11756715734447434 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 2651 0.11366540266965772 No Hit CCATAATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATAT 2594 0.1112214464447726 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 2546 0.10916337804486931 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 2538 0.10882036664488545 No Hit TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC 2512 0.10770557959493784 No Hit CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC 2508 0.1075340738949459 No Hit AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA 2502 0.10727681534495799 No Hit GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA 2476 0.10616202829501038 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 2466 0.10573326404503054 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2457 0.10534737622004868 No Hit CATAATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAG 2447 0.10491861197006883 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 2429 0.1041468363201051 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 2428 0.10410395989510711 No Hit CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC 2400 0.10290341999516354 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 2389 0.10243177932018571 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 2384 0.10221739719519579 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2370 0.101617127245224 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 2363 0.1013169922702381 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.287642499798481E-5 0.0 3 0.0 0.0 0.0 4.287642499798481E-5 0.0 4 0.0 0.0 0.0 4.287642499798481E-5 0.0 5 0.0 0.0 0.0 4.287642499798481E-5 0.0 6 0.0 0.0 0.0 4.287642499798481E-5 0.0 7 0.0 0.0 0.0 4.287642499798481E-5 0.0 8 0.0 0.0 0.0 8.575284999596962E-5 0.0 9 0.0 0.0 0.0 2.5725854998790885E-4 0.0 10-11 0.0 0.0 0.0 3.644496124828709E-4 0.0 12-13 0.0 0.0 0.0 5.359553124748101E-4 0.0 14-15 0.0 0.0 0.0 6.645845874687646E-4 0.0 16-17 0.0 0.0 0.0 8.575284999596961E-4 0.0 18-19 0.0 0.0 0.0 0.0013506073874365214 0.0 20-21 0.0 0.0 0.0 0.0022938887373921872 0.0 22-23 0.0 0.0 0.0 0.004845036024772284 0.0 24-25 0.0 0.0 0.0 0.010826297311991164 0.0 26-27 0.0 0.0 0.0 0.015049625174292668 0.0 28-29 0.0 0.0 0.0 0.021352459648996437 0.0 30-31 0.0 0.0 0.0 0.03940343457314804 0.0 32-33 0.0 0.0 0.0 0.07526956408396232 0.0 34-35 0.0 0.0 0.0 0.12082576564432118 0.0 36-37 0.0 0.0 0.0 0.1901998212910606 0.0 38-39 0.0 0.0 0.0 0.31756424174757447 0.0 40-41 0.0 0.0 0.0 0.4845679171147253 0.0 42-43 0.0 0.0 0.0 0.6414313179698528 0.0 44-45 0.0 0.0 0.0 0.8093782746869592 0.0 46-47 0.0 0.0 0.0 1.0070600321401681 0.0 48-49 0.0 0.0 0.0 1.2216779774675812 0.0 50-51 0.0 0.0 0.0 1.4580985849064694 0.0 52-53 0.0 0.0 0.0 1.7020868813575019 0.0 54-55 0.0 0.0 0.0 1.9433739630336615 0.0 56-57 0.0 0.0 0.0 2.1992604674216345 0.0 58-59 0.0 0.0 0.0 2.495300743820221 0.0 60-61 0.0 0.0 0.0 2.829801173441999 0.0 62-63 0.0 0.0 0.0 3.1614074443764135 0.0 64-65 0.0 0.0 0.0 3.5075488233851453 0.0 66-67 0.0 0.0 0.0 3.8630587012559365 0.0 68-69 0.0 0.0 0.0 4.259515564999803 0.0 70-71 0.0 0.0 0.0 4.712869444715995 0.0 72-73 0.0 0.0 0.0 5.182066163468942 0.0 74-75 0.0 0.0 0.0 5.64616058764713 0.0 76-77 0.0 0.0 0.0 6.134608821224173 0.0 78-79 0.0 0.0 0.0 6.677531552761156 0.0 80-81 0.0 0.0 0.0 7.27574343433304 0.0 82-83 0.0 0.0 0.0 7.915695515640462 0.0 84-85 0.0 0.0 0.0 8.54128399457356 0.0 86-87 0.0 0.0 0.0 9.196907409217745 0.0 88-89 0.0 0.0 0.0 9.893413495097509 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGATCC 55 8.198258E-7 51.814674 3 ACATATA 795 0.0 44.81213 2 GGTATCA 3845 0.0 43.979984 1 GACATAT 820 0.0 43.445908 1 CATTCCG 540 0.0 42.22027 9 TAAGGCG 45 9.6293073E-4 42.22027 5 GTATCAA 7095 0.0 41.776653 1 TTCGATC 70 4.3115742E-6 40.71502 2 ACCCTTG 1440 0.0 40.24119 7 TATCACG 60 8.2925E-5 39.58405 2 TAACTTT 1830 0.0 39.451725 6 CATATAG 935 0.0 38.607014 3 TCACGCA 75 6.9275466E-6 37.99824 4 CCTTGGC 1575 0.0 37.09352 9 AACCCTT 1675 0.0 34.878986 6 AACTTTA 2205 0.0 34.465527 7 CTTAACT 125 5.660695E-9 34.198418 4 CCCTTGG 1715 0.0 34.06548 8 AACGCAG 8540 0.0 33.760147 6 TCAACGC 8550 0.0 33.609554 4 >>END_MODULE