FastQCFastQC Report
Fri 10 Feb 2017
SRR2568712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332284
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC157860.6768472450181882No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT103140.44222744742921527No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA92560.3968641897813474No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC90440.3877743876817746No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC88630.38001375475713933No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT77250.33122038310943264No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG72160.3093962827854584No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC70470.30215016696079894No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA65740.28186961793675214No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT64330.2758240420120363No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA59590.25550061656299144No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT58570.251127221213197No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT56590.24263768906359603No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG53640.22998914368919052No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG49890.2139104843149462No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT49280.21129502239006914No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG46520.19946112909062533No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT46060.19748881354071804No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC45240.19397294669088327No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC44840.19225788969096388No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG44270.18981393346607875No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA43820.1878844943411694No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT43380.18599793164125808No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC43370.18595505521626013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43330.18578354951626816No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT42660.18291082904140318No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA42160.18076700779150395No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT41650.17858031011660672No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA40440.17339226269185054No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC40040.17167720569193118No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT39760.1704766657919876No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT39340.16867585594207224TruSeq Adapter, Index 1 (95% over 21bp)
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC39320.16859010309207625No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA39100.1676468217421206No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT39030.1673466867671347No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC38040.1631019206923342No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT37690.16160124581740473No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA36150.1549982763677151No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG35420.1518682973428622No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC35040.15023899319293876No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC34230.146766002768102No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC34020.14586559784314432No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA33530.14376465301824307No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA33350.14299287736827934No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT32040.13737606569354333No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT31810.13638990791858968No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA31110.13338855816873074No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT30480.1306873433938577No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC30360.13017282629388188No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA30310.12995844416889196No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC30280.129829814893898No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA29440.12622819519406728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.12438450891915392No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA28820.12356985684419221No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG28730.12318396901921036No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA27940.11979673144436956No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA27750.11898207936940784No Hit
CCATAATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATAT27170.11649524671952473No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC27150.11640949386952876No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT27100.11619511174453884No Hit
CATAATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAG26580.11396553764464361No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA26260.1125934920447081No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC26190.11229335706972221No Hit
TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA25710.11023528866981895No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT25680.11010665939482499No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC25560.10959214229484916No Hit
CTCATAACCCTAGTATTATTTTTCCCAGACATACTAGGAGACCCAGACAA25170.10791996171992776No Hit
CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC25100.10761982674494187No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC24890.10671942181998419No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC24780.10624778114500637No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG24690.10586189332002448No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA24540.10521874694505472No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT24420.1047042298450789No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG24180.10367519564512725No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA24140.10350368994513533No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC24140.10350368994513533No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC24130.10346081352013733No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT24060.10316067854515144No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG23960.1027319142951716No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC23830.10217452077019779No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC23710.10166000367022197No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA23680.10153137439522802No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG23610.10123123942024213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT19600.047.2676356
GACATAT10000.046.1460881
GGTATCA36300.043.7726631
ACATATA10550.043.731922
CATTCCG4000.042.748039
GTATGGT4650.041.946341
GTATCAA68800.041.2117421
CATATAG11300.040.3625073
AACTTTA23850.039.0422137
ATCTATA25350.037.3454971
GGCATTC4600.037.1801767
CTTCGGC3450.035.7954569
ATAACTT26750.035.3439145
CTCGAGT1502.5465852E-1134.831739
ACTTTAT26900.034.784648
CCTTTAG5100.034.5139541
CTTAATA48850.034.3707772
ACTTAAT49050.034.140261
TCTTCGG3800.033.7484478
CTATAAC29750.033.21713
Produced by FastQC (version 0.11.5)