##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568712_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332284 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.688353133666396 34.0 31.0 34.0 31.0 34.0 2 32.80913302153597 34.0 31.0 34.0 31.0 34.0 3 32.8659099835183 34.0 31.0 34.0 31.0 34.0 4 36.28102023595754 37.0 37.0 37.0 35.0 37.0 5 36.35166772142672 37.0 37.0 37.0 35.0 37.0 6 36.36628215088729 37.0 37.0 37.0 35.0 37.0 7 36.29988157531415 37.0 37.0 37.0 35.0 37.0 8 36.29712805130079 37.0 37.0 37.0 35.0 37.0 9 38.12353298311869 39.0 39.0 39.0 37.0 39.0 10-11 38.04070237586846 39.0 39.0 39.0 35.0 39.0 12-13 38.060863299666764 39.0 39.0 39.0 36.0 39.0 14-15 39.48994676463072 41.0 39.0 41.0 37.0 41.0 16-17 39.48500075462508 41.0 39.0 41.0 37.0 41.0 18-19 39.599257208813334 41.0 39.5 41.0 37.0 41.0 20-21 39.59190797518656 41.0 40.0 41.0 37.0 41.0 22-23 39.53630368342792 41.0 39.5 41.0 37.0 41.0 24-25 39.50394162975006 41.0 39.0 41.0 37.0 41.0 26-27 39.41912005570505 41.0 39.0 41.0 36.0 41.0 28-29 39.34065319660899 41.0 39.0 41.0 36.0 41.0 30-31 39.28819710635583 41.0 39.0 41.0 36.0 41.0 32-33 39.145453555398916 41.0 39.0 41.0 35.5 41.0 34-35 39.09501758790953 41.0 39.0 41.0 35.0 41.0 36-37 39.01677068487371 41.0 39.0 41.0 35.0 41.0 38-39 38.85441009756959 40.0 38.5 41.0 35.0 41.0 40-41 38.832803380720364 40.0 38.0 41.0 35.0 41.0 42-43 38.753413177811964 40.0 38.0 41.0 35.0 41.0 44-45 38.667547134054004 40.0 38.0 41.0 35.0 41.0 46-47 38.501461871710305 40.0 38.0 41.0 34.5 41.0 48-49 38.399542465668844 40.0 38.0 41.0 34.0 41.0 50-51 38.42706205590743 40.0 38.0 41.0 34.5 41.0 52-53 38.569374484410986 40.0 39.0 41.0 35.0 41.0 54-55 38.4782477176879 40.0 38.0 41.0 34.5 41.0 56-57 38.370441164112094 40.0 38.0 41.0 34.0 41.0 58-59 38.237326157534845 40.0 38.0 41.0 34.0 41.0 60-61 38.213105265053485 40.0 38.0 41.0 34.0 41.0 62-63 38.04456704243566 40.0 38.0 41.0 34.0 41.0 64-65 37.82498979541085 40.0 37.0 41.0 33.5 41.0 66-67 37.5819441371634 39.0 37.0 41.0 33.0 41.0 68-69 37.24039332259708 39.0 36.5 41.0 33.0 41.0 70-71 36.81551774998242 39.0 36.0 41.0 32.0 41.0 72-73 36.44739319911297 37.5 35.5 40.0 32.0 41.0 74-75 36.043244947870846 37.0 35.0 39.5 32.0 41.0 76-77 34.20179103402502 35.5 33.0 38.0 29.0 39.0 78-79 35.11073587093168 36.0 35.0 39.0 31.0 40.0 80-81 34.92676535104644 36.0 35.0 37.5 31.0 39.0 82-83 34.63394295034395 35.5 35.0 37.0 31.0 39.0 84-85 34.230281132143425 35.0 35.0 36.5 31.0 39.0 86-87 33.977619149297425 35.0 35.0 36.0 31.0 37.0 88-89 33.76851511222476 35.0 34.0 36.0 31.0 37.0 90-91 33.62432083742803 35.0 34.0 36.0 31.0 37.0 92-93 33.47586293092951 35.0 34.0 35.0 30.5 36.0 94-95 33.343460101771484 35.0 34.0 35.0 30.0 36.0 96-97 33.233967647164754 35.0 34.0 35.0 30.0 36.0 98-99 33.16378322708555 35.0 34.0 35.0 30.0 36.0 100-101 32.125470140000104 34.5 32.5 35.0 27.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2765865388268338 1101 2 0.16524535203139123 1101 3 0.167039175038461 1101 4 0.1756462241148924 1101 5 0.11890740888814832 1101 6 0.13222890033708978 1101 7 0.13937193321618935 1101 8 0.15261032594302293 1101 9 0.14936454066166505 1101 10-11 0.18600027316900736 1101 12-13 0.2330382489050109 1101 14-15 0.3101582359829891 1101 16-17 0.29239708672022857 1101 18-19 0.2684449650672107 1101 20-21 0.31259418280245654 1101 22-23 0.23266137508425544 1101 24-25 0.30410666210269 1101 26-27 0.26354343034110883 1101 28-29 0.21322444694555287 1101 30-31 0.17963163932893877 1101 32-33 0.28605990434338935 1101 34-35 0.32781594866557384 1101 36-37 0.18884774084549605 1101 38-39 0.19474387089668 1101 40-41 0.1352452489444005 1101 42-43 0.062493493678964285 1101 44-45 0.05861684379378573 1101 46-47 0.09663701925543222 1101 48-49 -0.029876556835965573 1101 50-51 0.13200241640799248 1101 52-53 0.2695040551942256 1101 54-55 0.18262449228487299 1101 56-57 0.21248872966186383 1101 58-59 0.22244065042293926 1101 60-61 0.2886983622671764 1101 62-63 0.21425525689470248 1101 64-65 0.11809285380689971 1101 66-67 0.14862814652498457 1101 68-69 0.17829667003172744 1101 70-71 0.10864224482461537 1101 72-73 0.06695598874738096 1101 74-75 -0.045573706199128594 1101 76-77 -0.10451686828255191 1101 78-79 0.1119458110337348 1101 80-81 0.21656764149120278 1101 82-83 0.1101420514947904 1101 84-85 0.11295148620738615 1101 86-87 0.11224153083806243 1101 88-89 0.0736150324829552 1101 90-91 0.05820973523037054 1101 92-93 0.043161941827733585 1101 94-95 0.17254493610965227 1101 96-97 0.06667051371378818 1101 98-99 0.04392917732285184 1101 100-101 -0.001893087404447158 1102 1 0.2786252748105298 1102 2 0.07269746856125181 1102 3 0.09737342043788999 1102 4 0.14538709004117578 1102 5 0.14527684930877172 1102 6 0.1486729155739539 1102 7 0.15302609921221944 1102 8 0.16433305784924102 1102 9 0.14475055922489588 1102 10-11 0.18467241223226694 1102 12-13 0.1785154740780328 1102 14-15 0.26680827085610304 1102 16-17 0.27737482157724713 1102 18-19 0.2595925588222414 1102 20-21 0.23760357171817503 1102 22-23 0.2567506502599599 1102 24-25 0.1628571315484848 1102 26-27 0.1623309189402775 1102 28-29 0.17682227944730045 1102 30-31 0.14102978300617508 1102 32-33 0.17783253163025847 1102 34-35 0.2531472432629158 1102 36-37 0.0780719481527612 1102 38-39 0.14271586510238166 1102 40-41 0.20967252508582135 1102 42-43 0.17973422547787976 1102 44-45 0.19777398741737784 1102 46-47 0.18097630784387064 1102 48-49 0.22289987966814095 1102 50-51 0.1104615612118991 1102 52-53 0.21597382288562272 1102 54-55 0.2748638828101164 1102 56-57 0.3516324605487071 1102 58-59 0.28373685730099396 1102 60-61 0.2961156056815284 1102 62-63 0.2658004041665549 1102 64-65 0.22783586577106263 1102 66-67 0.0748312690100974 1102 68-69 0.07841201956765076 1102 70-71 0.1635539890169042 1102 72-73 0.13066648823770066 1102 74-75 0.1437470728126229 1102 76-77 0.08866677890882357 1102 78-79 0.04623681376993005 1102 80-81 0.18711327162156977 1102 82-83 0.1596887009931578 1102 84-85 0.2066528650367232 1102 86-87 0.24933311300449645 1102 88-89 0.14444769517945844 1102 90-91 0.09150014780615123 1102 92-93 0.05693682245437515 1102 94-95 -0.006461448353036303 1102 96-97 -0.04727068484836394 1102 98-99 0.05476666860457158 1102 100-101 0.12119559761084986 1103 1 0.30857210550276903 1103 2 0.18812900507559505 1103 3 0.20189171419145424 1103 4 0.14559079598033264 1103 5 0.1573320799102973 1103 6 0.1309315228929222 1103 7 0.21031067846686113 1103 8 0.17176112290344747 1103 9 0.2130229133796746 1103 10-11 0.24512506466192008 1103 12-13 0.2442585366447858 1103 14-15 0.3289839645994235 1103 16-17 0.3092029029096466 1103 18-19 0.2718136577277548 1103 20-21 0.3041416818757625 1103 22-23 0.3193952563278799 1103 24-25 0.3190706656704805 1103 26-27 0.38768695851272383 1103 28-29 0.3331406386794029 1103 30-31 0.33032289824841143 1103 32-33 0.3747478155657049 1103 34-35 0.3420190461542134 1103 36-37 0.39354957570787974 1103 38-39 0.33090768796646586 1103 40-41 0.305462327991755 1103 42-43 0.3496895656370711 1103 44-45 0.3132829138985045 1103 46-47 0.3972499432198404 1103 48-49 0.3610481721048231 1103 50-51 0.41471124204973364 1103 52-53 0.44780729979621015 1103 54-55 0.46479612257863323 1103 56-57 0.4734483835400951 1103 58-59 0.5420045520539247 1103 60-61 0.4916090633143497 1103 62-63 0.47147047096197525 1103 64-65 0.45654836650266617 1103 66-67 0.4794181277593452 1103 68-69 0.42903875255420587 1103 70-71 0.35964496120864453 1103 72-73 0.46523348851570034 1103 74-75 0.3873890926610315 1103 76-77 0.31583342362738165 1103 78-79 0.3796298185399891 1103 80-81 0.44560399122709526 1103 82-83 0.3357821972180872 1103 84-85 0.3750376575538752 1103 86-87 0.35963302027371213 1103 88-89 0.35014393967413326 1103 90-91 0.3310050623548264 1103 92-93 0.3213480459886071 1103 94-95 0.19885230451850333 1103 96-97 0.33084320391859023 1103 98-99 0.2410842139274294 1103 100-101 0.3095361418143483 1104 1 0.14426737021485536 1104 2 0.05400557823175234 1104 3 0.0994489278594557 1104 4 0.11520361500585352 1104 5 0.1312956863927539 1104 6 0.0010925297128636657 1104 7 0.11914847639978632 1104 8 0.09676244119073374 1104 9 0.1167958515783667 1104 10-11 0.1404958988741143 1104 12-13 0.2057221431272538 1104 14-15 0.19928481972844025 1104 16-17 0.20327665229444847 1104 18-19 0.10822727915080321 1104 20-21 0.18878791948385754 1104 22-23 0.17365013838511345 1104 24-25 0.20619967765811253 1104 26-27 0.0932399141128144 1104 28-29 0.17456012648085562 1104 30-31 0.18886649840308678 1104 32-33 0.10195506790346798 1104 34-35 0.128344671901246 1104 36-37 0.10394207377439812 1104 38-39 0.1778778243652539 1104 40-41 0.10196359354753781 1104 42-43 0.20774550935500002 1104 44-45 0.09198719130129973 1104 46-47 0.1891045756813483 1104 48-49 0.22761508836720878 1104 50-51 0.2366494099819505 1104 52-53 0.1800292070942504 1104 54-55 0.24174180474835794 1104 56-57 0.11263205611268745 1104 58-59 0.11934044725887816 1104 60-61 0.23303602141039192 1104 62-63 0.21172327451959916 1104 64-65 0.1054876945276888 1104 66-67 0.13353700651207134 1104 68-69 -0.022623717564613344 1104 70-71 0.11305438168955106 1104 72-73 0.0547495891976979 1104 74-75 0.09739478326779505 1104 76-77 0.07326504846039228 1104 78-79 0.108322560929075 1104 80-81 0.05835226866503973 1104 82-83 0.0806201137968614 1104 84-85 0.17290049415655773 1104 86-87 0.1501772751777395 1104 88-89 0.1717824169644402 1104 90-91 0.06441156063225861 1104 92-93 0.10598516544301617 1104 94-95 0.10512592383229702 1104 96-97 0.10432717354525067 1104 98-99 0.1405990842079632 1104 100-101 0.14252001916089796 1105 1 0.253984044769588 1105 2 0.09725666076198536 1105 3 0.14720533148658888 1105 4 0.11564356210513438 1105 5 0.12228650420038889 1105 6 0.10246383606131104 1105 7 0.1635451733898705 1105 8 0.18858460814919198 1105 9 0.31057916546529185 1105 10-11 0.30255179989574543 1105 12-13 0.29026303959246746 1105 14-15 0.3547682809538415 1105 16-17 0.34167286505071814 1105 18-19 0.26916579240701566 1105 20-21 0.3573655252518364 1105 22-23 0.37252326061035035 1105 24-25 0.3059132740431423 1105 26-27 0.264686544368665 1105 28-29 0.2541282580561841 1105 30-31 0.3160962551790121 1105 32-33 0.3631810797757069 1105 34-35 0.36206754172873445 1105 36-37 0.4544982734879923 1105 38-39 0.43409262464314224 1105 40-41 0.41825550623459407 1105 42-43 0.3958943127013228 1105 44-45 0.3074649098543958 1105 46-47 0.3579140783440309 1105 48-49 0.20555495334141227 1105 50-51 0.21780225144891574 1105 52-53 0.35046296135410415 1105 54-55 0.26644880071023636 1105 56-57 0.27659265067394045 1105 58-59 0.3926275309911773 1105 60-61 0.42981842157975336 1105 62-63 0.41262750207542354 1105 64-65 0.308767587752925 1105 66-67 0.28959278830025426 1105 68-69 0.24537864763770756 1105 70-71 0.3019511924380822 1105 72-73 0.32236967735745026 1105 74-75 0.3473192890268919 1105 76-77 0.26978696004027114 1105 78-79 0.35666990013729105 1105 80-81 0.29160745136596944 1105 82-83 0.18924409894378158 1105 84-85 0.2352240621080668 1105 86-87 0.30318019396393936 1105 88-89 0.31344513959945175 1105 90-91 0.17102950380657234 1105 92-93 0.15880047404473174 1105 94-95 0.2704181613696619 1105 96-97 0.15393218232350847 1105 98-99 0.17799130264364038 1105 100-101 0.24384849880057402 1106 1 0.27319424561210326 1106 2 0.20893196788858148 1106 3 0.19844592557027596 1106 4 0.1672987581184202 1106 5 0.1499935920247566 1106 6 0.10396924982819655 1106 7 0.12899867198781578 1106 8 0.17716074833369788 1106 9 0.2118511174695712 1106 10-11 0.22295395208852398 1106 12-13 0.22820624029599657 1106 14-15 0.26950066416748797 1106 16-17 0.2915248346434609 1106 18-19 0.2037677687151387 1106 20-21 0.2707173424345797 1106 22-23 0.1786925373226822 1106 24-25 0.21607674399268006 1106 26-27 0.27762387641109143 1106 28-29 0.23128065521446928 1106 30-31 0.2883430355906498 1106 32-33 0.26474115092373296 1106 34-35 0.36008212403773143 1106 36-37 0.25759003598999186 1106 38-39 0.2570157901463901 1106 40-41 0.22552458755846772 1106 42-43 0.20808501090264997 1106 44-45 0.2057553484331578 1106 46-47 0.23997968355189414 1106 48-49 0.2517155642894764 1106 50-51 0.16134000883788246 1106 52-53 0.282077584772928 1106 54-55 0.29013705276017987 1106 56-57 0.26660763473804394 1106 58-59 0.19726044477850735 1106 60-61 0.22017991942556847 1106 62-63 0.3021335184749745 1106 64-65 0.3242517435358039 1106 66-67 0.21163734808060042 1106 68-69 0.19698572646930046 1106 70-71 0.2054256733973432 1106 72-73 0.20343369773484454 1106 74-75 0.11039402365246076 1106 76-77 0.10688086912916361 1106 78-79 0.20245853724147622 1106 80-81 0.13945438266934929 1106 82-83 0.13923399667141467 1106 84-85 0.21023687973110583 1106 86-87 0.17221748828342953 1106 88-89 0.22059416154765898 1106 90-91 0.20045970060817808 1106 92-93 0.18739422605204936 1106 94-95 0.1737693128535298 1106 96-97 0.26075057383152966 1106 98-99 0.195670162500015 1106 100-101 0.2929636448514046 1107 1 0.14148979333305078 1107 2 0.06790773168456354 1107 3 0.05529171036209135 1107 4 0.05009005882784834 1107 5 0.08243730762978174 1107 6 0.022279714156866248 1107 7 0.04269943545544663 1107 8 0.03749645644101918 1107 9 0.05517176074123853 1107 10-11 0.09592468364024853 1107 12-13 0.09127825467599138 1107 14-15 0.1467830458712811 1107 16-17 0.19841921567930143 1107 18-19 0.12149817401532204 1107 20-21 0.11214517138330393 1107 22-23 0.11673317053737975 1107 24-25 0.08594838611417543 1107 26-27 0.12244190317842651 1107 28-29 0.10487666469354906 1107 30-31 0.03703712880162158 1107 32-33 0.14683287211263263 1107 34-35 0.0769165947233148 1107 36-37 0.19239470348097854 1107 38-39 0.20970912908361328 1107 40-41 0.21907667278765075 1107 42-43 0.09923861818870705 1107 44-45 0.1451800364509026 1107 46-47 0.13430489937362466 1107 48-49 0.2209485129181772 1107 50-51 0.15165809846136824 1107 52-53 0.20170427555634518 1107 54-55 0.17576673061774528 1107 56-57 0.233543144332522 1107 58-59 0.1673314440786413 1107 60-61 0.23485542222783096 1107 62-63 0.36156015784769124 1107 64-65 0.30064560202129087 1107 66-67 0.10566526371451346 1107 68-69 0.1752658322177325 1107 70-71 0.2623701517194803 1107 72-73 0.17520866147528835 1107 74-75 0.22409206648094937 1107 76-77 0.13415565180638822 1107 78-79 0.2596841795468592 1107 80-81 0.2704193839921416 1107 82-83 0.19219602217787468 1107 84-85 0.29782851367192364 1107 86-87 0.24405624632104406 1107 88-89 0.15724314093272085 1107 90-91 0.22396996304666317 1107 92-93 0.14188412497561842 1107 94-95 0.13134920113845538 1107 96-97 0.2677496525668204 1107 98-99 0.2812733844872852 1107 100-101 0.21456373716549848 1108 1 0.11011431329497867 1108 2 -0.01993376548717407 1108 3 -0.03958555853581913 1108 4 0.04149177000630999 1108 5 0.01724820545142336 1108 6 0.04868044064183863 1108 7 0.0918041849603668 1108 8 8.904498137241035E-5 1108 9 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72-73 -0.48086553489374495 2316 74-75 -0.4678737699700193 2316 76-77 -0.6249203822034417 2316 78-79 -0.45564906627976853 2316 80-81 -0.3586380739746957 2316 82-83 -0.46321002057597127 2316 84-85 -0.5225841469843928 2316 86-87 -0.5461154448275352 2316 88-89 -0.649060560900331 2316 90-91 -0.5616081252080036 2316 92-93 -0.4502976168004196 2316 94-95 -0.37668199283895376 2316 96-97 -0.49911963537481796 2316 98-99 -0.4852325676322451 2316 100-101 -0.4962823362123636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 0.0 12 3.0 13 12.0 14 101.0 15 327.0 16 1001.0 17 2093.0 18 3272.0 19 4505.0 20 5999.0 21 8472.0 22 9215.0 23 8165.0 24 7700.0 25 8651.0 26 10847.0 27 14239.0 28 19024.0 29 22620.0 30 24512.0 31 29220.0 32 36596.0 33 47277.0 34 65343.0 35 95585.0 36 164146.0 37 415349.0 38 942873.0 39 385085.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.33463791274156 26.66372471573292 14.47062466281211 22.53101270871341 2 11.377661636168764 24.97258257741371 44.53658224604384 19.113173540373683 3 13.580207213186728 35.22546996849441 32.29195072298228 18.902372095336588 4 8.493734039250795 21.618850877508912 47.28875214167743 22.598662941562864 5 9.361467128360012 42.98160086850486 37.67337082447935 9.983561178655773 6 20.521128922573734 47.62769669574643 21.97087221283197 9.880302168847864 7 22.91307831135144 33.87989274391667 29.8791053072204 13.327923637511493 8 21.630684770808358 39.20560274820733 25.0466924268228 14.1170200541615 9 26.29812664323899 16.2382883045118 24.005052557921765 33.45853249432745 10-11 19.79398735316968 29.98588508089066 34.06527678447393 16.154850781465722 12-13 21.266406664025478 28.956057667076568 31.94407284876113 17.833462820136827 14-15 20.353739081518373 29.24148602828815 29.264103342474584 21.14067154771889 16-17 18.44618837157053 32.87168286538003 29.75004330518925 18.932085457860193 18-19 18.325491235201202 31.435880021472514 31.61021556551432 18.628413177811964 20-21 19.480796506771902 31.188311543534148 31.057902896902778 18.272989052791168 22-23 19.76105825877123 31.071108835802157 29.905534660444438 19.262298244982173 24-25 19.513918545082845 32.08466464632952 29.45329556777819 18.94812124080944 26-27 19.080973843665696 31.750678733807717 30.90811410617232 18.260233316354267 28-29 18.92451380052932 29.4306886383199 32.73164619106677 18.91315137008401 30-31 19.992676706610343 31.058546043277747 30.046876795450295 18.90190045466161 32-33 19.19339583001041 30.572841900900578 31.510527877394008 18.723234391695005 34-35 18.82746698086511 31.65156987742488 31.18556745233428 18.335395689375737 36-37 19.934763477408936 30.14557627560126 29.95314675644996 19.966513490539846 38-39 18.64363430868625 31.61310972420168 31.162028295010387 18.581227672101683 40-41 18.116725921886015 30.68534964009529 32.61669676591701 18.581227672101683 42-43 18.804999734166163 30.24310075445357 30.767265050053936 20.184634461326322 44-45 19.04960973877967 30.77256028854119 30.619898777335862 19.55793119534328 46-47 19.332594143766368 29.83639642513519 30.932725174121163 19.89828425697728 48-49 18.22312767723135 30.523604870505665 30.723623436001667 20.529644016261315 50-51 18.50462893884278 30.211800964205043 29.9127807762691 21.370789320683073 52-53 18.57299539850207 29.425104318342022 31.67705991208618 20.324840371069733 54-55 19.150969607474906 30.296932106038543 30.30812285296302 20.243975433523534 56-57 19.640704133801886 30.160284939569966 29.65462182135623 20.54438910527191 58-59 18.138700089697483 29.47833539997702 30.94914684489539 21.433817665430112 60-61 19.97276060719878 31.661131320199427 29.242343556788107 19.123764515813683 62-63 18.69056916610159 30.985809811469935 31.144731519214773 19.1788895032137 64-65 18.804477341242055 30.876049457247145 30.176175471438427 20.14329773007237 66-67 19.594376156591565 30.945030708095583 29.42293905887962 20.03765407643323 68-69 19.158001341174575 30.7999583241149 29.96933478084144 20.07270555386908 70-71 19.05919261976672 29.69910611229164 31.106138875025515 20.13556239291613 72-73 19.33529535854124 30.62851693876046 29.405359724630447 20.63082797806785 74-75 18.919479788910785 30.703057603619456 28.988772379350024 21.38869022811973 76-77 18.117026056861 30.845986166350237 31.149529817123472 19.88745795966529 78-79 18.780345789792324 29.851424612096984 29.700671101804065 21.667558496306626 80-81 18.513718740942355 31.20078858320856 29.270577682649286 21.0149149931998 82-83 19.282664546856214 30.434822688832064 29.4170221122299 20.86549065208182 84-85 19.231577286471115 29.872434060346 29.030748399423057 21.865240253759836 86-87 17.385168358570397 31.133579787024225 31.19510745689633 20.28614439750905 88-89 18.821571472427888 29.38104879165659 30.586305098349943 21.21107463756558 90-91 18.840115526239515 29.951948390504757 30.520296842065548 20.68763924119018 92-93 19.635301704252143 30.253948489978065 29.63854316198199 20.472206643787807 94-95 18.83638527726469 28.681176906414485 29.770431045275792 22.712006771045033 96-97 19.640532628101894 28.708810762325687 30.303492199063236 21.347164410509183 98-99 18.99118952836525 28.966786384919658 30.361900996942264 21.680123089772827 100-101 18.537396152764344 29.26127398519555 30.933613232075846 21.26771662996426 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107.0 1 76.0 2 106.0 3 170.0 4 128.5 5 103.0 6 190.5 7 394.5 8 641.0 9 704.5 10 568.5 11 484.5 12 504.5 13 505.0 14 534.5 15 599.5 16 637.5 17 686.5 18 749.0 19 820.5 20 915.5 21 1007.0 22 1139.5 23 1350.0 24 1548.0 25 1809.0 26 2404.5 27 3347.0 28 4507.5 29 6734.0 30 12096.5 31 19948.5 32 42154.5 33 74227.5 34 108284.0 35 151305.5 36 218266.5 37 274554.5 38 259476.0 39 226335.5 40 183014.5 41 130609.5 42 102266.5 43 85263.5 44 73559.0 45 60008.5 46 47209.5 47 37113.0 48 33214.0 49 29684.5 50 24855.5 51 18966.0 52 14035.0 53 11419.0 54 9960.0 55 8988.0 56 7925.5 57 6996.5 58 6136.5 59 5368.5 60 4627.5 61 3433.0 62 2156.0 63 1469.5 64 1038.0 65 737.0 66 546.5 67 408.0 68 315.0 69 231.0 70 181.0 71 132.5 72 79.5 73 72.0 74 59.0 75 38.5 76 27.5 77 19.5 78 13.0 79 9.0 80 6.5 81 6.5 82 8.5 83 5.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021352459648996434 2 0.1098065244198391 3 0.0 4 0.0 5 0.0 6 0.0033443611498428154 7 0.02864145189865385 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0015649895124264454 30-31 0.0 32-33 0.0 34-35 0.0 36-37 6.431463749697721E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.1438212498992405E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.5006748749294682E-4 64-65 3.0013497498589364E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.0719106249496202E-4 100-101 5.145170999758177E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2332284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.318307991705419 #Duplication Level Percentage of deduplicated Percentage of total 1 64.28476664415808 6.633100214093343 2 12.884056889585912 2.658833343388032 3 5.649832028346062 1.7489012090962925 4 3.2075899149735783 1.3238760261514233 5 2.1420282653744858 1.1051053684536227 6 1.4696112132969437 0.9098340676117052 7 1.0921170328961958 0.7888159935887299 8 0.8573005372850199 0.7076712788129097 9 0.6681953135730087 0.6205180539654435 >10 5.482450490208322 11.666989674915563 >50 0.9349870749050156 6.7986911553199425 >100 1.014688072941899 22.595894352832254 >500 0.17568501262929836 12.430045441005017 >1k 0.13069250939496585 25.09968629289881 >5k 0.005142000369638001 3.7580533565812155 >10k+ 8.570000616063335E-4 1.153984171285689 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 15786 0.6768472450181882 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 10314 0.44222744742921527 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 9256 0.3968641897813474 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 9044 0.3877743876817746 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 8863 0.38001375475713933 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 7725 0.33122038310943264 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 7216 0.3093962827854584 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 7047 0.30215016696079894 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 6574 0.28186961793675214 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 6433 0.2758240420120363 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 5959 0.25550061656299144 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 5857 0.251127221213197 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 5659 0.24263768906359603 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 5364 0.22998914368919052 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 4989 0.2139104843149462 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 4928 0.21129502239006914 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 4652 0.19946112909062533 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 4606 0.19748881354071804 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 4524 0.19397294669088327 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 4484 0.19225788969096388 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 4427 0.18981393346607875 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 4382 0.1878844943411694 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 4338 0.18599793164125808 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 4337 0.18595505521626013 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4333 0.18578354951626816 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 4266 0.18291082904140318 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 4216 0.18076700779150395 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 4165 0.17858031011660672 No Hit TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA 4044 0.17339226269185054 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 4004 0.17167720569193118 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 3976 0.1704766657919876 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 3934 0.16867585594207224 TruSeq Adapter, Index 1 (95% over 21bp) GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 3932 0.16859010309207625 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 3910 0.1676468217421206 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 3903 0.1673466867671347 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 3804 0.1631019206923342 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 3769 0.16160124581740473 No Hit ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA 3615 0.1549982763677151 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 3542 0.1518682973428622 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 3504 0.15023899319293876 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 3423 0.146766002768102 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 3402 0.14586559784314432 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 3353 0.14376465301824307 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 3335 0.14299287736827934 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 3204 0.13737606569354333 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 3181 0.13638990791858968 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 3111 0.13338855816873074 No Hit AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT 3048 0.1306873433938577 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 3036 0.13017282629388188 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 3031 0.12995844416889196 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 3028 0.129829814893898 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2944 0.12622819519406728 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2901 0.12438450891915392 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 2882 0.12356985684419221 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2873 0.12318396901921036 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2794 0.11979673144436956 No Hit GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA 2775 0.11898207936940784 No Hit CCATAATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATAT 2717 0.11649524671952473 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 2715 0.11640949386952876 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 2710 0.11619511174453884 No Hit CATAATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAG 2658 0.11396553764464361 No Hit AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA 2626 0.1125934920447081 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 2619 0.11229335706972221 No Hit TAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCA 2571 0.11023528866981895 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2568 0.11010665939482499 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 2556 0.10959214229484916 No Hit CTCATAACCCTAGTATTATTTTTCCCAGACATACTAGGAGACCCAGACAA 2517 0.10791996171992776 No Hit CTACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTC 2510 0.10761982674494187 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 2489 0.10671942181998419 No Hit ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC 2478 0.10624778114500637 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 2469 0.10586189332002448 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 2454 0.10521874694505472 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 2442 0.1047042298450789 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2418 0.10367519564512725 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2414 0.10350368994513533 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 2414 0.10350368994513533 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 2413 0.10346081352013733 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 2406 0.10316067854515144 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 2396 0.1027319142951716 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2383 0.10217452077019779 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 2371 0.10166000367022197 No Hit GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA 2368 0.10153137439522802 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 2361 0.10123123942024213 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.287642499798481E-5 0.0 3 0.0 0.0 0.0 4.287642499798481E-5 0.0 4 0.0 0.0 0.0 4.287642499798481E-5 0.0 5 0.0 0.0 0.0 4.287642499798481E-5 0.0 6 0.0 0.0 0.0 4.287642499798481E-5 0.0 7 0.0 0.0 0.0 4.287642499798481E-5 0.0 8 0.0 0.0 0.0 8.575284999596962E-5 0.0 9 0.0 0.0 0.0 2.1438212498992403E-4 0.0 10-11 0.0 0.0 0.0 3.4301139998387843E-4 0.0 12-13 0.0 0.0 0.0 4.2876424997984806E-4 0.0 14-15 0.0 0.0 0.0 5.359553124748101E-4 0.0 16-17 0.0 0.0 0.0 7.288992249657418E-4 0.0 18-19 0.0 0.0 0.0 0.0012434163249415596 0.0 20-21 0.0 0.0 0.0 0.0021652594623982327 0.0 22-23 0.0 0.0 0.0 0.004823597812273291 0.0 24-25 0.0 0.0 0.0 0.01086917373698915 0.0 26-27 0.0 0.0 0.0 0.015156816236787631 0.0 28-29 0.0 0.0 0.0 0.021588279986485354 0.0 30-31 0.0 0.0 0.0 0.03953206384814199 0.0 32-33 0.0 0.0 0.0 0.07559113727144721 0.0 34-35 0.0 0.0 0.0 0.12093295670681616 0.0 36-37 0.0 0.0 0.0 0.19013550665356363 0.0 38-39 0.0 0.0 0.0 0.3178643767225604 0.0 40-41 0.0 0.0 0.0 0.4846322317522223 0.0 42-43 0.0 0.0 0.0 0.6413241269073577 0.0 44-45 0.0 0.0 0.0 0.8092067689869673 0.0 46-47 0.0 0.0 0.0 1.0070171557151701 0.0 48-49 0.0 0.0 0.0 1.2212706514301002 0.0 50-51 0.0 0.0 0.0 1.4567479775190328 0.0 52-53 0.0 0.0 0.0 1.7001360040200937 0.0 54-55 0.0 0.0 0.0 1.9409300068087763 0.0 56-57 0.0 0.0 0.0 2.196366308734271 0.0 58-59 0.0 0.0 0.0 2.491677685907891 0.0 60-61 0.0 0.0 0.0 2.8260280480421764 0.0 62-63 0.0 0.0 0.0 3.159478005251504 0.0 64-65 0.0 0.0 0.0 3.5063911599101996 0.0 66-67 0.0 0.0 0.0 3.863037263043437 0.0 68-69 0.0 0.0 0.0 4.260330217074764 0.0 70-71 0.0 0.0 0.0 4.71426292852843 0.0 72-73 0.0 0.0 0.0 5.183159512306392 0.0 74-75 0.0 0.0 0.0 5.647404003972072 0.0 76-77 0.0 0.0 0.0 6.135680731849122 0.0 78-79 0.0 0.0 0.0 6.677960317011136 0.0 80-81 0.0 0.0 0.0 7.276922536020484 0.0 82-83 0.0 0.0 0.0 7.91807515722785 0.0 84-85 0.0 0.0 0.0 8.545957524898341 0.0 86-87 0.0 0.0 0.0 9.202867232292466 0.0 88-89 0.0 0.0 0.0 9.902460420772083 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACTTT 1960 0.0 47.267635 6 GACATAT 1000 0.0 46.146088 1 GGTATCA 3630 0.0 43.772663 1 ACATATA 1055 0.0 43.73192 2 CATTCCG 400 0.0 42.74803 9 GTATGGT 465 0.0 41.94634 1 GTATCAA 6880 0.0 41.211742 1 CATATAG 1130 0.0 40.362507 3 AACTTTA 2385 0.0 39.042213 7 ATCTATA 2535 0.0 37.345497 1 GGCATTC 460 0.0 37.180176 7 CTTCGGC 345 0.0 35.795456 9 ATAACTT 2675 0.0 35.343914 5 CTCGAGT 150 2.5465852E-11 34.83173 9 ACTTTAT 2690 0.0 34.78464 8 CCTTTAG 510 0.0 34.513954 1 CTTAATA 4885 0.0 34.370777 2 ACTTAAT 4905 0.0 34.14026 1 TCTTCGG 380 0.0 33.748447 8 CTATAAC 2975 0.0 33.2171 3 >>END_MODULE