FastQCFastQC Report
Fri 10 Feb 2017
SRR2568711_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568711_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015120
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC34080.11303032715115817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44850.038.6578141
TCAACGC47500.034.798754
CAACGCA48300.034.4190565
GGTATCA27050.033.891981
ATCAACG49800.033.382343
AACGCAG52100.032.365026
TATCAAC57500.029.4922052
GTACATG93450.027.6519931
ACGCAGA60150.027.4807687
CGCAGAG60700.027.0752628
ACATGGG88100.026.0944123
TACATGG98650.024.7980212
CGTATAC3500.024.4276963
CATGGGG49900.022.2737524
GCAGAGT76100.021.4713349
TAATACT36100.018.8151154
TAGGTCG4400.018.3516165
GTATTAT20500.018.3054371
AGAGTAC69350.018.21867810-11
GCGTTAT2101.9569981E-518.095791
Produced by FastQC (version 0.11.5)