FastQCFastQC Report
Fri 10 Feb 2017
SRR2568711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015120
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC38810.12871792830799436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44800.041.4163931
TCAACGC49750.036.3865474
CAACGCA50500.035.7520645
ATCAACG52100.034.562923
GGTATCA25000.033.8739361
AACGCAG54200.033.5744136
TATCAAC58200.030.8930442
ACGCAGA62700.028.566777
CGCAGAG62300.028.5138348
CGTATAC2400.027.715693
TACCGAC1205.3764506E-627.714317
ACATGGG84100.027.5132523
GTACATG92650.026.7533361
TACATGG96500.024.8917792
GGACCGT2202.1664164E-923.7563086
CATGGGG48600.023.3652554
GCAGAGT77200.022.825949
TAATACT33900.020.7429714
AGAGTAC71750.019.03215810-11
CATGGGA39550.018.5004864
Produced by FastQC (version 0.11.5)