FastQCFastQC Report
Fri 10 Feb 2017
SRR2568710_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568710_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3494588
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34800.039.3104931
GTACATG108550.034.9632841
TCAACGC37100.034.953464
TACATGG104950.034.850562
GGTATCA20900.034.318311
CAACGCA38300.034.106365
ATCAACG40000.032.299663
ACATGGG109750.032.070283
AACGCAG43150.030.493026
TATCAAC46750.028.1448942
CATGGGG77350.025.9765514
ACGCAGA50900.025.756857
CGCAGAG53950.024.212678
GTATATA20900.022.7273581
GAGTACA72800.019.7047441
ATGGGGG44750.018.8941965
AGTACAT72150.018.2366432
CATGGGA46350.017.524594
GTATTAG10150.017.3153361
AGAGTAC60100.017.18991910-11
Produced by FastQC (version 0.11.5)