FastQCFastQC Report
Fri 10 Feb 2017
SRR2568710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3494588
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41200.036.7216381
GTACATG106400.034.0726171
TACATGG104600.034.0133972
ATCAACG43700.033.162293
TCAACGC43500.033.0963064
GGTATCA26650.032.1341931
CAACGCA45100.031.9221525
ACATGGG108500.031.7055233
AACGCAG47700.030.5806066
TATCAAC50500.029.0094452
CATGGGG73550.026.8742754
ACGCAGA58200.024.5725927
CGCAGAG61650.023.0360938
ATGGGGG41600.022.0439825
TATACCG3059.094947E-1221.809935
ATTATAC12000.019.401753
GTATATA21050.019.2113971
GAGTACA71750.019.0968931
CATGGGA46600.018.9650124
AGTACAT70600.018.12292
Produced by FastQC (version 0.11.5)