FastQCFastQC Report
Fri 10 Feb 2017
SRR2568709_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568709_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244229
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33100.040.0411341
GGTATCA20750.037.545331
TCAACGC37750.033.470784
CAACGCA38050.033.0820475
ATCAACG39650.032.1057663
AACGCAG41150.030.705276
TATCAAC44850.028.7030032
GTACATG62600.026.939171
ACGCAGA47000.026.5802487
TACATGG60600.026.1814882
CGCAGAG48850.025.5736278
ACATGGG60150.025.506943
CGGACTA1200.004764409719.792015
GCAGAGT62700.019.4700369
GTATAAT14900.019.4479471
CATGGGG39400.019.2896754
AGAGTAC53150.018.94628510-11
CATGGGA25050.018.5831584
GTATAGA9450.018.0967621
ACCGTGC4201.8189894E-1218.0955528
Produced by FastQC (version 0.11.5)