##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568709_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244229 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79588402074833 33.0 31.0 34.0 30.0 34.0 2 31.937761253419325 34.0 31.0 34.0 30.0 34.0 3 31.972165050892755 34.0 31.0 34.0 30.0 34.0 4 35.3316551029329 37.0 35.0 37.0 33.0 37.0 5 35.37158730236531 37.0 35.0 37.0 33.0 37.0 6 35.482059094682405 37.0 35.0 37.0 33.0 37.0 7 35.459242350045386 37.0 35.0 37.0 33.0 37.0 8 35.479376658977316 37.0 35.0 37.0 33.0 37.0 9 37.23928841486319 39.0 38.0 39.0 34.0 39.0 10-11 37.20229419546757 39.0 37.5 39.0 34.0 39.0 12-13 37.17109773556977 39.0 37.0 39.0 34.0 39.0 14-15 38.53669701264889 41.0 38.0 41.0 34.0 41.0 16-17 38.50391337069435 41.0 38.0 41.0 33.5 41.0 18-19 38.48635388812818 41.0 38.5 41.0 33.5 41.0 20-21 38.48037210106455 41.0 39.0 41.0 34.0 41.0 22-23 38.4142801380786 40.0 38.5 41.0 33.5 41.0 24-25 38.35049074760196 40.0 38.0 41.0 33.0 41.0 26-27 38.25504193199535 40.0 38.0 41.0 33.0 41.0 28-29 38.12642292742853 40.0 38.0 41.0 33.0 41.0 30-31 38.041387933227846 40.0 38.0 41.0 33.0 41.0 32-33 37.925722375033914 40.0 38.0 41.0 32.0 41.0 34-35 37.77512232486079 40.0 38.0 41.0 32.0 41.0 36-37 37.66372794398433 40.0 38.0 41.0 31.0 41.0 38-39 37.556109247318346 40.0 38.0 41.0 31.0 41.0 40-41 37.42581282926118 40.0 37.0 41.0 31.0 41.0 42-43 37.274825563701384 40.0 37.0 41.0 30.0 41.0 44-45 37.085732115572874 40.0 37.0 41.0 30.0 41.0 46-47 37.14875465026074 40.0 37.0 41.0 30.0 41.0 48-49 37.337642905425426 40.0 37.0 41.0 30.0 41.0 50-51 36.248215756948156 39.0 35.5 40.0 29.0 40.5 52-53 36.400369792922206 39.0 36.0 40.0 29.5 40.5 54-55 37.01517759551275 40.0 36.5 41.0 30.0 41.0 56-57 36.99905869677292 40.0 36.0 41.0 30.0 41.0 58-59 36.84819263096591 40.0 36.0 41.0 29.5 41.0 60-61 36.64281541678679 39.0 35.5 41.0 29.0 41.0 62-63 36.3874896456645 39.0 35.0 41.0 29.0 41.0 64-65 36.215152286152616 39.0 35.0 41.0 28.5 41.0 66-67 36.09546062367076 38.5 35.0 41.0 29.0 41.0 68-69 35.80291672552133 37.5 35.0 40.0 29.0 41.0 70-71 35.447622992127805 37.0 35.0 40.0 28.0 41.0 72-73 35.088907370860994 36.5 34.0 39.0 28.0 41.0 74-75 34.7268872739814 36.0 34.0 39.0 28.0 41.0 76-77 34.35329081836123 36.0 34.0 38.5 27.5 40.0 78-79 33.98874958838871 35.0 34.0 37.0 27.0 39.0 80-81 33.61903999101696 35.0 34.0 37.0 26.0 39.0 82-83 33.29310957126033 35.0 34.0 36.5 26.0 39.0 84-85 32.9984471281674 35.0 33.0 36.0 26.0 37.0 86-87 32.75103454237514 35.0 33.0 36.0 26.0 37.0 88-89 32.53443030991936 35.0 33.0 35.0 25.5 36.5 90-91 32.35543765809995 35.0 33.0 35.0 25.0 36.0 92-93 32.19792966760522 35.0 33.0 35.0 25.0 36.0 94-95 32.05312358943762 35.0 33.0 35.0 25.0 36.0 96-97 31.950371820344536 35.0 32.5 35.0 24.5 35.5 98-99 31.81153260206512 35.0 32.0 35.0 24.0 35.0 100-101 30.49656942317384 34.0 29.5 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15173231287663214 1101 2 0.2335299183013504 1101 3 0.3403647526806921 1101 4 0.13799045636146445 1101 5 0.08025252696623397 1101 6 0.18357037291496425 1101 7 0.11162647142725035 1101 8 0.242306110809281 1101 9 0.25520804830345867 1101 10-11 0.24881278548988206 1101 12-13 0.3320451980340593 1101 14-15 0.35552487567129987 1101 16-17 0.34776376695824496 1101 18-19 0.4108584552613479 1101 20-21 0.2779342131433822 1101 22-23 0.2404131463313135 1101 24-25 0.3365450129793359 1101 26-27 0.31507972467498746 1101 28-29 0.39721170829962915 1101 30-31 0.3996666442983212 1101 32-33 0.33264699841047474 1101 34-35 0.1536170358108393 1101 36-37 0.2789751335544608 1101 38-39 0.3616195625979728 1101 40-41 0.24842354641004505 1101 42-43 0.22411317376160866 1101 44-45 0.38046287784833055 1101 46-47 0.39574099224141435 1101 48-49 0.43864545187328474 1101 50-51 0.3602434667815615 1101 52-53 0.3489533892468586 1101 54-55 0.3452934835613064 1101 56-57 0.3322004619754395 1101 58-59 0.3799353068697542 1101 60-61 0.3777334828580763 1101 62-63 0.3204414903335078 1101 64-65 0.45471902619467386 1101 66-67 0.47583344110960724 1101 68-69 0.43716682375847427 1101 70-71 0.46533139095802056 1101 72-73 0.22901263418918205 1101 74-75 0.2573150458534954 1101 76-77 0.23093877070533608 1101 78-79 0.2086725048934781 1101 80-81 0.20134639291742928 1101 82-83 0.3269178804075281 1101 84-85 0.18884657958530937 1101 86-87 0.2526828611086813 1101 88-89 0.15076928749103047 1101 90-91 0.2580452716867896 1101 92-93 0.11432006190901944 1101 94-95 -0.02609735707770966 1101 96-97 0.011075864209917796 1101 98-99 -0.04579346187503219 1101 100-101 -0.15251239626580926 1102 1 0.2536834756222248 1102 2 0.22830095730520839 1102 3 0.36905981831208123 1102 4 0.34194730407207174 1102 5 0.33941473811160705 1102 6 0.32406335721989876 1102 7 0.3983014996042513 1102 8 0.40781401868612477 1102 9 0.49085803720506505 1102 10-11 0.47876003039377935 1102 12-13 0.36485496720896293 1102 14-15 0.5255731052076911 1102 16-17 0.5045009246050398 1102 18-19 0.47843939274181935 1102 20-21 0.3149774279286035 1102 22-23 0.3839801709481989 1102 24-25 0.43508799825402633 1102 26-27 0.37721544405580687 1102 28-29 0.6436017124157303 1102 30-31 0.5227229702765399 1102 32-33 0.47175279091140254 1102 34-35 0.4875305027990393 1102 36-37 0.3546399059511245 1102 38-39 0.3285269459408937 1102 40-41 0.3143343368529301 1102 42-43 0.2720962826487323 1102 44-45 0.43068937133691776 1102 46-47 0.4642589360856846 1102 48-49 0.5269931053705719 1102 50-51 0.5224877995955381 1102 52-53 0.31837159622010347 1102 54-55 0.48981216873535516 1102 56-57 0.452538004079301 1102 58-59 0.4207432485805427 1102 60-61 0.3365549840077833 1102 62-63 0.4196557669658887 1102 64-65 0.4431987829350632 1102 66-67 0.3620924410471176 1102 68-69 0.43525833057590546 1102 70-71 0.5586794646858593 1102 72-73 0.4924027956223611 1102 74-75 0.46433084867701524 1102 76-77 0.3461757915078252 1102 78-79 0.5024055744513376 1102 80-81 0.398257590660684 1102 82-83 0.4715141466052657 1102 84-85 0.5003697321333007 1102 86-87 0.41013570369893415 1102 88-89 0.4150672546527119 1102 90-91 0.3823222057709401 1102 92-93 0.37327345079899743 1102 94-95 0.3122609638103313 1102 96-97 0.3651442346420666 1102 98-99 0.4446715070602316 1102 100-101 0.022397900675560578 1103 1 0.12369749147337927 1103 2 0.09210057720471809 1103 3 0.25926520459436375 1103 4 0.1935070484093373 1103 5 0.2334719524277844 1103 6 0.2434248321361352 1103 7 0.1823980644045804 1103 8 0.17963304614561082 1103 9 0.1449121251886467 1103 10-11 0.14368976687877222 1103 12-13 0.21280373666096608 1103 14-15 0.25386918915214807 1103 16-17 0.24561709823313294 1103 18-19 0.22627141068016243 1103 20-21 0.12152891704698021 1103 22-23 0.21429593863901886 1103 24-25 0.2310006423660056 1103 26-27 0.29724126464724776 1103 28-29 0.12302602985674582 1103 30-31 0.18968705025395138 1103 32-33 0.2123907004478056 1103 34-35 0.2330328562552353 1103 36-37 0.16357583111676632 1103 38-39 0.02909333049519347 1103 40-41 0.11568212232010211 1103 42-43 0.12365369843517016 1103 44-45 0.10241645141112343 1103 46-47 0.09372140126372841 1103 48-49 0.13542733919837957 1103 50-51 0.11237958070204002 1103 52-53 0.05959609808233779 1103 54-55 0.20571874241657184 1103 56-57 0.2611338595828059 1103 58-59 0.3618642210481653 1103 60-61 0.2690869448865101 1103 62-63 0.32028658150916556 1103 64-65 0.2578110063285308 1103 66-67 0.16028595131521683 1103 68-69 0.1305494338513853 1103 70-71 0.22041392322894637 1103 72-73 0.19344987406051217 1103 74-75 0.16391185126595786 1103 76-77 0.09287289402115562 1103 78-79 0.09370390149893382 1103 80-81 0.2123803121736998 1103 82-83 0.10236908517808985 1103 84-85 0.13568890760762287 1103 86-87 0.24796995704607383 1103 88-89 -0.004428770004821558 1103 90-91 0.11965529423521559 1103 92-93 0.11332780524978858 1103 94-95 0.002658971866715376 1103 96-97 0.12381808647414516 1103 98-99 0.0040431749546208096 1103 100-101 -0.1955372457416935 1104 1 0.24762259515552643 1104 2 0.21883826455093924 1104 3 0.315487261408947 1104 4 0.35121983340653884 1104 5 0.3478429016159268 1104 6 0.23656303021930825 1104 7 0.26208754261539013 1104 8 0.31264255970005195 1104 9 0.35599554849915904 1104 10-11 0.2801405765753202 1104 12-13 0.21565486095892794 1104 14-15 0.37994364416502435 1104 16-17 0.3338408261225254 1104 18-19 0.3259415106994865 1104 20-21 0.3801636371566559 1104 22-23 0.2983358992942229 1104 24-25 0.372488212766406 1104 26-27 0.38136281768768043 1104 28-29 0.41388228898317436 1104 30-31 0.24334081022131926 1104 32-33 0.24254006746938472 1104 34-35 0.2523144509940991 1104 36-37 0.33412519696153 1104 38-39 0.16669525968438137 1104 40-41 0.23629479091085415 1104 42-43 0.20381664784299147 1104 44-45 0.17736141087480917 1104 46-47 0.25109771573961126 1104 48-49 0.23746185316960577 1104 50-51 0.09677690031372777 1104 52-53 0.17645796864660213 1104 54-55 0.28634525817656 1104 56-57 0.33887934037475276 1104 58-59 0.20370591416861572 1104 60-61 0.19512893946728127 1104 62-63 0.3014397211978803 1104 64-65 0.2523784441020851 1104 66-67 0.13032438407455516 1104 68-69 0.14527823233486714 1104 70-71 0.07737403808591381 1104 72-73 0.19901404323525895 1104 74-75 0.1502588562272109 1104 76-77 0.09543707202200835 1104 78-79 0.0832110423036525 1104 80-81 0.06464857067641816 1104 82-83 -0.029249827387623384 1104 84-85 0.08343271144070741 1104 86-87 0.0274124025424598 1104 88-89 0.007733889122398807 1104 90-91 0.024259478002456092 1104 92-93 0.06642032336535664 1104 94-95 0.1772514757624606 1104 96-97 -0.04388319517354944 1104 98-99 0.09020296854157905 1104 100-101 -0.057162248315972164 1105 1 0.18507527362885412 1105 2 0.17283225134585933 1105 3 0.38266335242609983 1105 4 0.13096671942533789 1105 5 0.23522615853561035 1105 6 0.16563984009081878 1105 7 0.27935536331133903 1105 8 0.2908218837305583 1105 9 0.26926621930781636 1105 10-11 0.381138448979506 1105 12-13 0.3802625209697865 1105 14-15 0.3272598435264129 1105 16-17 0.3871955353011529 1105 18-19 0.4475517577869397 1105 20-21 0.4933380833657779 1105 22-23 0.3669270579516066 1105 24-25 0.4529725987435853 1105 26-27 0.45632144776902095 1105 28-29 0.5314211367518595 1105 30-31 0.5038524320886069 1105 32-33 0.3020072029396417 1105 34-35 0.4469495133736032 1105 36-37 0.39656763700428144 1105 38-39 0.28564318186564464 1105 40-41 0.39499778985538114 1105 42-43 0.32066227894650723 1105 44-45 0.4356568703389456 1105 46-47 0.33588091072926574 1105 48-49 0.5337846750475492 1105 50-51 0.45968614619921055 1105 52-53 0.3742071787059018 1105 54-55 0.4911028820615684 1105 56-57 0.44211135486505526 1105 58-59 0.41806202509659585 1105 60-61 0.527720111442946 1105 62-63 0.47288631987471064 1105 64-65 0.40094989169963213 1105 66-67 0.43210570451579144 1105 68-69 0.3948778514319571 1105 70-71 0.4179345380542898 1105 72-73 0.3182812712675869 1105 74-75 0.2917972511742519 1105 76-77 0.3863563332568134 1105 78-79 0.18573785367347995 1105 80-81 0.19688247969256167 1105 82-83 0.2634913750536114 1105 84-85 0.19032492324136996 1105 86-87 0.1579918477693596 1105 88-89 0.2792857022402373 1105 90-91 0.17915178303110224 1105 92-93 0.14264285856724257 1105 94-95 0.14348962665195586 1105 96-97 0.09799648079261658 1105 98-99 0.2545349400804078 1105 100-101 0.12810440037101145 1106 1 0.08504391956223145 1106 2 0.06965822967524815 1106 3 0.23174599844528387 1106 4 0.18742582217096526 1106 5 0.13030555033272861 1106 6 0.16832550842200078 1106 7 0.09133481704648716 1106 8 0.17497729868787815 1106 9 0.23930207295803285 1106 10-11 0.1696377773423947 1106 12-13 0.06962040068678021 1106 14-15 0.2330812485626481 1106 16-17 0.20354486580931308 1106 18-19 0.20368917881044268 1106 20-21 0.22401281187806887 1106 22-23 0.22482789769052403 1106 24-25 0.14744196141992205 1106 26-27 0.09327755097561408 1106 28-29 0.1065158865409046 1106 30-31 0.03688542646798254 1106 32-33 0.18697034265886003 1106 34-35 0.12887141864472085 1106 36-37 0.03462949362708656 1106 38-39 -0.07810502627695115 1106 40-41 0.08496569869316062 1106 42-43 0.12688435594989755 1106 44-45 0.1455709161906924 1106 46-47 -0.002697792928593401 1106 48-49 -0.016192607005933723 1106 50-51 0.08166490684446615 1106 52-53 0.05336079492992951 1106 54-55 0.1825567536287096 1106 56-57 0.2498859532304536 1106 58-59 0.0747503975785122 1106 60-61 0.12693295934931825 1106 62-63 0.17938640293548502 1106 64-65 0.0973120498715474 1106 66-67 0.06943442605097516 1106 68-69 0.1600356724013352 1106 70-71 0.1708515113857274 1106 72-73 0.2027808108578668 1106 74-75 -0.006498187744178097 1106 76-77 0.0921060089897594 1106 78-79 0.11961652773749876 1106 80-81 0.10191387226418414 1106 82-83 -0.00675790627817463 1106 84-85 0.004952750470557987 1106 86-87 0.16487720079964419 1106 88-89 0.1617371061873314 1106 90-91 0.18456833555796237 1106 92-93 0.041106949359821954 1106 94-95 0.14509000422091844 1106 96-97 0.0790642352803701 1106 98-99 0.14352944266738987 1106 100-101 0.02629015506339627 1107 1 0.13420671545387108 1107 2 0.08111375490857853 1107 3 0.22369115633875936 1107 4 0.10380213072056677 1107 5 0.16779815563734246 1107 6 0.10620009992564405 1107 7 0.16111711536857598 1107 8 0.2169887665799095 1107 9 0.207528093610577 1107 10-11 0.17105405185563427 1107 12-13 0.22406889253807805 1107 14-15 0.24317559610825157 1107 16-17 0.2208297643877799 1107 18-19 0.18973869950497857 1107 20-21 0.08682134717925294 1107 22-23 0.09801170974481721 1107 24-25 0.15343607150467875 1107 26-27 0.2679057858375984 1107 28-29 0.3203728765930265 1107 30-31 0.2366862657486024 1107 32-33 0.08238521621763795 1107 34-35 8.143062167462745E-4 1107 36-37 0.05660823566596207 1107 38-39 0.16840177038253756 1107 40-41 -0.02971995868949051 1107 42-43 0.09471140913725407 1107 44-45 0.3365563556778568 1107 46-47 0.1999418710638281 1107 48-49 0.20128203876393513 1107 50-51 0.18131346846661955 1107 52-53 0.12090405793559 1107 54-55 0.14424037233653308 1107 56-57 0.2941284272532769 1107 58-59 0.42556766715703276 1107 60-61 0.41782509416211866 1107 62-63 0.33373549900606747 1107 64-65 0.2731580445538313 1107 66-67 0.2957154272442821 1107 68-69 0.2862187544457697 1107 70-71 0.2960829964989742 1107 72-73 0.32010954694407445 1107 74-75 0.4242334741034455 1107 76-77 0.36032916866810893 1107 78-79 0.16007254856538822 1107 80-81 0.23059181609125545 1107 82-83 0.2867090130155887 1107 84-85 0.27118827140319723 1107 86-87 0.2782000192837515 1107 88-89 0.1810809832872664 1107 90-91 0.3488357831167619 1107 92-93 0.32012862539232856 1107 94-95 0.1078961399953755 1107 96-97 0.17421248450260407 1107 98-99 0.33362779557687006 1107 100-101 0.4869753860686039 1108 1 0.022884839142403024 1108 2 -0.014682953247142194 1108 3 0.15478907616472526 1108 4 0.22212129539964565 1108 5 0.10991598483833798 1108 6 0.01524006065878325 1108 7 0.12600386555756415 1108 8 0.03070576118559387 1108 9 0.05585860646946372 1108 10-11 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-0.9323504285564752 2316 54-55 -0.8864950280756503 2316 56-57 -1.2083857162438179 2316 58-59 -1.2429542087635568 2316 60-61 -1.0646157584195919 2316 62-63 -0.834084155394045 2316 64-65 -1.0013933676763145 2316 66-67 -0.7584181416783338 2316 68-69 -0.6228175248491681 2316 70-71 -0.6770926636307237 2316 72-73 -0.7315213210674614 2316 74-75 -0.50431717569721 2316 76-77 -0.7742483142271439 2316 78-79 -0.47820150398080585 2316 80-81 -0.4284644301323013 2316 82-83 -0.5206366971512324 2316 84-85 -0.6452480588227658 2316 86-87 -0.5094594511994401 2316 88-89 -0.3857634556005678 2316 90-91 -0.4557375958066956 2316 92-93 -0.3245037419819532 2316 94-95 -0.2906962836917941 2316 96-97 -0.36889818788218776 2316 98-99 -0.3486202686294071 2316 100-101 -0.20480819208487233 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 12.0 7 196.0 8 510.0 9 559.0 10 860.0 11 1665.0 12 3007.0 13 4323.0 14 5490.0 15 6223.0 16 7030.0 17 7861.0 18 9018.0 19 10184.0 20 11582.0 21 13186.0 22 14450.0 23 14911.0 24 15398.0 25 16493.0 26 18151.0 27 20152.0 28 23556.0 29 27545.0 30 32489.0 31 39720.0 32 49210.0 33 63837.0 34 89795.0 35 134494.0 36 234443.0 37 464358.0 38 678634.0 39 224768.0 40 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79611243805925 19.643862963887205 13.500677337706321 24.059347260347224 2 14.371941926686452 20.712592354928123 41.67419765959912 23.241268058786307 3 17.12817299993004 27.20726991113573 30.487668483639446 25.176888605294785 4 11.368092254770032 17.87694611045263 40.81409131012111 29.940870324656228 5 12.441827097031778 38.85009705138465 36.3624535017815 12.345622349802065 6 29.32469032487481 39.288435676071664 19.30417454278414 12.08269945626939 7 26.222152707037168 32.86303636981009 24.312668607744527 16.60214231540822 8 24.812322231376932 36.62879171031741 21.341592477114833 17.217293581190827 9 25.903238901938135 15.824823099886235 20.827356451222627 37.444581546953 10-11 23.58155281724268 26.697926658222237 30.48308161576056 19.23743890877452 12-13 24.677149333667238 24.25879475917152 29.89380384279297 21.170252064368274 14-15 21.964380640300078 25.78395520243255 27.027121563797635 25.22454259346974 16-17 22.0027011503728 28.55074058841589 27.4733772712143 21.97318098999701 18-19 21.87991609566342 28.16171302087917 28.61648076338242 21.341890120074993 20-21 22.81660913684774 27.405619422702582 28.305673495459516 21.47209794499016 22-23 22.47316552157742 27.37493321736139 27.923520009838604 22.228381251222583 24-25 22.14625606173422 27.481139167124514 28.136561742909837 22.23604302823143 26-27 22.25393421847679 27.637717698769602 27.97151802281125 22.136830059942355 28-29 22.281838582207005 27.584436788042225 27.980664514714608 22.153060115036155 30-31 22.513516211432226 27.483431165807776 27.956565411689628 22.04648721107037 32-33 22.33416168386419 27.555243048918133 27.92252003926533 22.188075227952353 34-35 22.474979157652804 27.64713404915452 27.861706626195453 22.016180166997216 36-37 22.490891636034387 27.470977220011246 27.915294721549078 22.122836422405296 38-39 22.37627922665577 27.732043148932796 27.855069919397636 22.036607705013797 40-41 22.522679280946818 27.575372210233446 27.816680026860002 22.08526848195973 42-43 22.388939240413826 27.55997401326607 27.790766688856984 22.26032005746312 44-45 22.49568056572057 27.550181744862073 27.718815132668734 22.23532255674862 46-47 22.46093388822136 27.536715583645048 27.629867793375436 22.37248273475816 48-49 22.37097034365428 27.57555786672513 27.75471064186645 22.29876114775414 50-51 22.519265016263333 27.603709809267034 27.536045248140894 22.340979926328732 52-53 22.528850665417835 27.64755735711463 27.529209363215607 22.29438261425193 54-55 22.458447867842363 27.549906894528142 27.700916439454264 22.29072879817523 56-57 22.4739320274357 27.684340590911177 27.543913744987698 22.29781363666542 58-59 22.511294524756607 27.636551350151876 27.577065442073874 22.275088683017643 60-61 22.60573675859282 27.642299426662788 27.62010917780672 22.131854636937675 62-63 22.51884799268516 27.60076009115925 27.638122996366864 22.242268919788728 64-65 22.427442220466567 27.451288378768695 27.64541299549902 22.475856405265716 66-67 22.41877422814713 27.58335351533301 27.534159194870778 22.463713061649084 68-69 22.45658566192174 27.553719387597088 27.632126888692767 22.357568061788406 70-71 22.42645551683881 27.3485665398223 27.676341891614904 22.54863605172398 72-73 22.29932863357527 27.471171613948485 27.686412571979062 22.543087180497174 74-75 22.37710590140311 27.452479225604876 27.63904663917987 22.531368233812145 76-77 22.278007280005742 27.387779945807672 27.673579656977964 22.66063311720863 78-79 22.288188950414597 27.444480933095512 27.588071449036615 22.67925866745328 80-81 22.369874408368112 27.515938213810376 27.46820880346914 22.64597857435237 82-83 22.49513253454463 27.374362795406913 27.62545672178151 22.50504794826694 84-85 22.46981325979359 27.372467874641355 27.601447680030805 22.556271185534253 86-87 22.294868346787176 27.4199259193732 27.68303848667491 22.602167247164708 88-89 22.296779529852227 27.40250673879072 27.657908968334983 22.64280476302207 90-91 22.37969417008664 27.352271263726497 27.679822026295863 22.588212539891003 92-93 22.410433221748075 27.24133075225577 27.69642838044513 22.651807645551028 94-95 22.2476344050797 27.283235603025307 27.749747122410135 22.719382869484857 96-97 22.287697986076218 27.18657844632526 27.806764921162074 22.718958646436445 98-99 22.21923389220799 27.183951791378814 27.683970954323563 22.912843362089635 100-101 22.389794078469176 27.020544022874326 27.77193233427499 22.817729564381505 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13.0 1 10.5 2 21.0 3 35.5 4 34.0 5 42.5 6 61.0 7 110.5 8 203.0 9 246.5 10 235.0 11 243.0 12 291.0 13 351.0 14 414.5 15 450.5 16 508.0 17 628.5 18 718.0 19 784.5 20 893.0 21 1147.0 22 1438.0 23 1830.0 24 2436.0 25 3379.0 26 5120.0 27 7906.0 28 12081.5 29 18500.5 30 26300.5 31 34546.0 32 43277.0 33 50349.5 34 56843.0 35 62794.0 36 67929.0 37 72610.0 38 75884.5 39 78869.0 40 80933.0 41 82271.0 42 83603.5 43 85896.0 44 88531.5 45 90293.5 46 90561.5 47 90161.5 48 90452.0 49 89287.5 50 85969.5 51 81309.5 52 76602.0 53 72527.0 54 67970.0 55 61701.0 56 54517.0 57 47878.5 58 41683.5 59 35184.5 60 28653.5 61 23072.5 62 18034.5 63 13317.0 64 9429.0 65 6736.5 66 4841.5 67 3539.0 68 2595.0 69 1851.5 70 1394.0 71 1054.5 72 774.5 73 568.0 74 416.5 75 293.5 76 201.0 77 144.5 78 112.0 79 79.0 80 56.0 81 45.5 82 36.0 83 26.5 84 19.5 85 14.5 86 9.5 87 8.0 88 8.5 89 7.0 90 5.0 91 4.0 92 4.5 93 4.0 94 1.5 95 1.0 96 1.5 97 3.5 98 2.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0066392511637627 2 0.007530425816616753 3 8.911746528540537E-4 4 4.010285937843241E-4 5 0.002539847760634053 6 0.004589549462198377 7 0.005124254253910808 8 0.004010285937843241 9 0.007396749618688645 10-11 0.006527854332155944 12-13 0.003564698611416215 14-15 0.0 16-17 0.0 18-19 2.896317621775674E-4 20-21 0.001359041345602432 22-23 3.564698611416215E-4 24-25 0.001247644513995675 26-27 0.003252787482917296 28-29 0.002896317621775674 30-31 0.01145159428917459 32-33 4.4558732642702685E-4 34-35 0.0 36-37 2.2279366321351343E-5 38-39 8.911746528540537E-5 40-41 0.0 42-43 3.1191112849891876E-4 44-45 5.124254253910809E-4 46-47 0.0023393334637418907 48-49 0.002450730295348648 50-51 0.003141390651310539 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0010916889497462156 64-65 0.0023170540974205393 66-67 0.0030077144533824313 68-69 0.008176527439935943 70-71 4.010285937843241E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014682102405770534 82-83 0.011050565695390266 84-85 0.016174819949301072 86-87 0.014370191277271615 88-89 0.014147397614058102 90-91 0.013456737258096211 92-93 0.00913454019175405 94-95 0.02470781725037864 96-97 0.010092552943572159 98-99 0.014036000782451347 100-101 0.014080559515094047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2244229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.72655426864865 #Duplication Level Percentage of deduplicated Percentage of total 1 71.88701235825842 42.93563544826537 2 15.847962621314043 18.93088399098857 3 5.608179505297068 10.048717127143455 4 2.5973778695541725 6.205297211284573 5 1.3375109599305754 3.994246046660294 6 0.8000499434136308 2.8670535817754104 7 0.4829409326663723 2.019107847641491 8 0.33624305823297523 1.6066111412006547 9 0.2268624038030507 1.2194738705033092 >10 0.8406342851062607 8.13258800645638 >50 0.027195658830636342 1.1137435758184602 >100 0.007730636526707867 0.7528385486564892 >500 1.4988353302391173E-4 0.05917289826594379 >1k 1.4988353302387492E-4 0.11463070533967452 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.4558732642702686E-5 5 0.0 8.911746528540537E-5 0.0 0.0 4.4558732642702686E-5 6 0.0 8.911746528540537E-5 0.0 0.0 4.4558732642702686E-5 7 0.0 8.911746528540537E-5 0.0 0.0 4.4558732642702686E-5 8 0.0 8.911746528540537E-5 0.0 0.0 8.911746528540537E-5 9 0.0 8.911746528540537E-5 0.0 0.0 8.911746528540537E-5 10-11 0.0 8.911746528540537E-5 0.0 0.0 8.911746528540537E-5 12-13 0.0 8.911746528540537E-5 0.0 0.0 1.3367619792810804E-4 14-15 0.0 8.911746528540537E-5 0.0 2.2279366321351343E-5 1.3367619792810804E-4 16-17 0.0 8.911746528540537E-5 0.0 4.4558732642702686E-5 1.3367619792810804E-4 18-19 0.0 8.911746528540537E-5 0.0 4.4558732642702686E-5 1.3367619792810804E-4 20-21 0.0 8.911746528540537E-5 0.0 4.4558732642702686E-5 1.3367619792810804E-4 22-23 0.0 8.911746528540537E-5 0.0 8.911746528540536E-5 1.7823493057081074E-4 24-25 0.0 8.911746528540537E-5 0.0 1.3367619792810804E-4 1.7823493057081074E-4 26-27 0.0 8.911746528540537E-5 0.0 2.8963176217756747E-4 1.7823493057081074E-4 28-29 0.0 8.911746528540537E-5 0.0 0.0010025714844608103 1.7823493057081074E-4 30-31 0.0 8.911746528540537E-5 0.0 0.0022947747310991884 1.7823493057081074E-4 32-33 0.0 8.911746528540537E-5 0.0 0.006438736866870538 1.7823493057081074E-4 34-35 0.0 8.911746528540537E-5 0.0 0.011028286329068914 1.7823493057081074E-4 36-37 0.0 8.911746528540537E-5 0.0 0.02038562018403648 1.7823493057081074E-4 38-39 0.0 8.911746528540537E-5 0.0 0.03807543704318944 1.7823493057081074E-4 40-41 0.0 8.911746528540537E-5 0.0 0.06146877168060835 1.7823493057081074E-4 42-43 0.0 8.911746528540537E-5 0.0 0.085797839703524 1.7823493057081074E-4 44-45 0.0 8.911746528540537E-5 0.0 0.11333513647671428 1.7823493057081074E-4 46-47 0.0 8.911746528540537E-5 0.0 0.1445930874255702 1.7823493057081074E-4 48-49 0.0 8.911746528540537E-5 0.0 0.18006183860916153 1.7823493057081074E-4 50-51 0.0 8.911746528540537E-5 0.0 0.2217019742637672 1.7823493057081074E-4 52-53 0.0 8.911746528540537E-5 0.0 0.26187167174116366 2.4507302953486474E-4 54-55 0.0 1.3367619792810804E-4 0.0 0.3070096679082215 3.1191112849891876E-4 56-57 0.0 1.3367619792810804E-4 0.0 0.360435588346822 3.1191112849891876E-4 58-59 0.0 1.3367619792810804E-4 0.0 0.4209686266419336 3.1191112849891876E-4 60-61 0.0 1.3367619792810804E-4 0.0 0.4916164972469387 3.1191112849891876E-4 62-63 0.0 1.3367619792810804E-4 0.0 0.5688590602830638 3.1191112849891876E-4 64-65 0.0 1.3367619792810804E-4 0.0 0.6479285313575397 3.341904948202701E-4 66-67 0.0 1.3367619792810804E-4 0.0 0.7391402570771521 3.564698611416215E-4 68-69 0.0 1.3367619792810804E-4 0.0 0.8386844658009498 3.564698611416215E-4 70-71 0.0 1.3367619792810804E-4 0.0 0.9515962943175584 4.0102859378432417E-4 72-73 0.0 1.3367619792810804E-4 0.0 1.0746006757777393 4.233079601056755E-4 74-75 0.0 1.3367619792810804E-4 0.0 1.2034645305804355 4.4558732642702685E-4 76-77 0.0 1.3367619792810804E-4 0.0 1.3422649827624542 4.6786669274837813E-4 78-79 0.0 1.3367619792810804E-4 0.0 1.500270248713478 4.901460590697295E-4 80-81 0.0 1.3367619792810804E-4 0.0 1.6741384234853038 5.124254253910808E-4 82-83 0.0 1.3367619792810804E-4 0.0 1.8699294947173395 5.347047917124322E-4 84-85 0.0 1.3367619792810804E-4 0.0 2.068371810541616 5.347047917124322E-4 86-87 0.0 1.3367619792810804E-4 0.0 2.27650565071568 5.347047917124322E-4 88-89 0.0 1.3367619792810804E-4 0.0 2.5099488510307992 5.347047917124322E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3310 0.0 40.041134 1 GGTATCA 2075 0.0 37.54533 1 TCAACGC 3775 0.0 33.47078 4 CAACGCA 3805 0.0 33.082047 5 ATCAACG 3965 0.0 32.105766 3 AACGCAG 4115 0.0 30.70527 6 TATCAAC 4485 0.0 28.703003 2 GTACATG 6260 0.0 26.93917 1 ACGCAGA 4700 0.0 26.580248 7 TACATGG 6060 0.0 26.181488 2 CGCAGAG 4885 0.0 25.573627 8 ACATGGG 6015 0.0 25.50694 3 CGGACTA 120 0.0047644097 19.79201 5 GCAGAGT 6270 0.0 19.470036 9 GTATAAT 1490 0.0 19.447947 1 CATGGGG 3940 0.0 19.289675 4 AGAGTAC 5315 0.0 18.946285 10-11 CATGGGA 2505 0.0 18.583158 4 GTATAGA 945 0.0 18.096762 1 ACCGTGC 420 1.8189894E-12 18.095552 8 >>END_MODULE