FastQCFastQC Report
Fri 10 Feb 2017
SRR2568709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244229
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.040.5131651
GTATCAA35050.040.1766431
TCAACGC40400.034.5759544
CAACGCA40600.034.405635
ATCAACG41750.033.2303283
AACGCAG43700.031.6387836
TATCAAC46200.030.1626592
ACGCAGA50100.027.121117
CGCAGAG50300.026.7233688
TATACCG2351.7644197E-1024.261725
GTACATG60650.023.9242881
TACATGG57900.023.7390462
ACATGGG57900.022.8125823
GCAGAGT66350.020.1158399
AGAGTAC57950.019.42529510-11
TCTATAC10100.018.8168453
CATGGGG37350.018.6997134
GTATTAC6200.017.6484321
CCTATAC9000.016.8933473
GTATTAT9700.016.6754321
Produced by FastQC (version 0.11.5)