FastQCFastQC Report
Fri 10 Feb 2017
SRR2568707_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568707_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53855
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6931.286788599015876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.9302757404140749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.6517500696314177No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3500.6498932318262No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2910.5403398013183548No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2560.47535047813573483No Hit
GTACATGGGATGTGGACACCTGAATATGCAAGGTGTCTTCCTGAAAAGCC1910.3546560207965834No Hit
GTACATGGGAGAAGTCTGTGGCTTTGTCAGATATGTCACCCAAGTAAATG1670.3100919134713583No Hit
CATTAAATCAAATGGAGGGAGGGTGTGAAGGAAAAGGGAGCTGAGACCAG1590.2952372110296166No Hit
TGTAAGAGGAGATCAGCTGCTCTTCACCACAATTACTACCAAATACTAGG1570.29152353541918113No Hit
CTTTTATGTTGCAAATGTAATAAACTGACATGCCTTTGACTTTCACACAC1560.28966669761396346No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1480.2748119951722217No Hit
GTACAATGGGGGGGTGTTACCAAGGCTGCAGAGAAGCAGATGAAGGACAA1430.26552780614613314No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1390.25810045492526223No Hit
GTACATGGGGATCAAAGACGCAGATCAGGCAGGACTTCCAGTGGAAGCAA1350.25067310370439144No Hit
GTAATAAACTGACATGCCTTTGACTTTCACACACTCACAACAAGATCATT1290.23953207687308514No Hit
GTACATGGGGGGACAAGCGAATAAGTGCACATGGTGGATGCCTTGGCGAT1220.22653421223656112No Hit
ACATTGGGGTCTGTCAGAACAAGACATCTGCTCATATGAAGCCAGACTCA1170.21725002321047257No Hit
CCCTAAAAGGGTGGGTTGCCCCATTCGGAAATCTGCGGATCAAAGTGTGT1160.21539318540525487No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.21539318540525487No Hit
GTGAGAGGCTAATCCTTTCCATCTCAGGGCTATCACATTCACACACACAT1160.21539318540525487No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1050.19496796954786No Hit
CCCCAATGTCATGCCTACAGTCCAAACACACACCCAGCCTCTGGGAAAAC960.17825642930090058No Hit
GTCCAAACACACACCCAGCCTCTGGGAAAACACACCCTGCAAGCTTCTGG950.17639959149568285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.17454275369046512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.17454275369046512No Hit
GTACATGGGCCACTAAAAATACAAAAATTAGCTGGGTGTGGTGGCGTGCA930.17268591588524743No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC930.17268591588524743No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA930.17268591588524743No Hit
TCTCTGTACAATGTCGATTATAATTATGCAAACAACAAAACTGTCAGTCT910.16897224027481197No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA880.16340172685915885No Hit
GAGTACAATGGGGGGGTGTTACCAAGGCTGCAGAGAAGCAGATGAAGGAC870.16154488905394113No Hit
CCCTCAGACCAGTCGGCCACCTCAGGCTGAGCAGCAGTGAACTCAGGAGC870.16154488905394113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.15968805124872343No Hit
GGCTAATCCTTTCCATCTCAGGGCTATCACATTCACACACACATTAAATC860.15968805124872343No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT850.15783121344350573No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC810.15040386222263485No Hit
GTACTTTTATGTTGCAAATGTAATAAACTGACATGCCTTTGACTTTCACA780.14483334880698173No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA770.14297651100176398No Hit
GTGTTTGGACTGTAGGCATGACATTGGGGTCTGTCAGAACAAGACATCTG760.14111967319654628No Hit
GTTTTACAACATCAGCCCATGATTTTGCAACAATCAAGTTGGCTTCAGAA760.14111967319654628No Hit
CCTATGTATTCAATATGCAATACCCTAAAAGGGTGGGTTGCCCCATTCGG750.13926283539132858No Hit
ATATAGGGACATATCTGCTAAAAGAACCCATGAATTTTTCAGATAGTGTC730.13554915978089313No Hit
CCTGAATATGCAAGGTGTCTTCCTGAAAAGCCCACACATTTCAAGTGGTT720.13369232197567543No Hit
CATTTCAAGTGGTTGGCCATGTAAGAGGAGATCAGCTGCTCTTCACCACA720.13369232197567543No Hit
TCTTTATCCTCAGCTTCTTCTTCCTCTTTACGCTTCTTGTCCTCTTCTTC710.1318354841704577No Hit
GTACATGGGTGCTCACCCTATTGGAGTCAGAAAGAGAAGCTCGAAGGTTG700.12997864636523998No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT670.12440813294958684No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG660.12255129514436915No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC660.12255129514436915No Hit
TTACATAACTCTGCAGTGTTTCTCAAGTGAGCTTTTCATTTTAATCAGAT640.11883761953393371No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.11883761953393371No Hit
ACCAAATACTAGGACTAGTTCCTGCTGTTGCTCTCGAAAGTGCCATATTC640.11883761953393371No Hit
GAGTACATGGGGATCAAAGACGCAGATCAGGCAGGACTTCCAGTGGAAGC640.11883761953393371No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA630.116980781728716No Hit
GCCCATGATTTTGCAACAATCAAGTTGGCTTCAGAAGCTCCACTTCGCCT620.11512394392349828No Hit
GTGTAAGCTTTTAAAGTTGTAGGTTAAACCTCCTGTTGTTATCTTTGCTG610.11326710611828056No Hit
GTATCAACGCAGAGTACATGGGATGTGGACACCTGAATATGCAAGGTGTC610.11326710611828056No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.11141026831306285No Hit
GAGTACATGGGATGTGGACACCTGAATATGCAAGGTGTCTTCCTGAAAAG600.11141026831306285No Hit
GTAGAATGCCATGCTGACTCCGGCTGATGGATGCTCTCTTCGCAGGCAGG580.10769659270262744No Hit
GACCAGAAGCTTGCAGGGTGTGTTTTCCCAGAGGCTGGGTGTGTGTTTGG580.10769659270262744No Hit
AGTAAATGCTTTGTAGATCTGATTAAAATGAAAAGCTCACTTGAGAAACA570.10583975489740971No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT560.103982917092192No Hit
TTTCAAGTGGTTGGCCATGTAAGAGGAGATCAGCTGCTCTTCACCACAAT560.103982917092192No Hit
AATGGAGGGAGGGTGTGAAGGAAAAGGGAGCTGAGACCAGAAGCTTGCAG560.103982917092192No Hit
GTACATGGGGGGGGGATGGAAAGAAGCCAACCAGCTGCTGACAAGCCAGA560.103982917092192No Hit
CATTAGGATAGGGTAGGCTAAGTTCCCTATGCTGCTAGCATCCTAAGATG550.10212607928697429No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATA550.10212607928697429No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA550.10212607928697429No Hit
TTCACACACACATTAAATCAAATGGAGGGAGGGTGTGAAGGAAAAGGGAG540.10026924148175656No Hit
GTTGTTATCTTTGCTGTTTGTGTTGCTTCCTTCTATCTGGAATGTGGTAG540.10026924148175656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATGG200.002064684971.238171
GAGGCTA407.455965E-659.365145
GTACCCA250.004998678356.9905321
ATATGGG250.004998678356.9905323
TGCGCTG250.004998678356.9905325
GCCACTA250.004998678356.9905329
AGGCTAA451.4980553E-552.769016
TTATTCT558.0538484E-751.809574
AAAAGTA654.3202817E-851.1453482
GGGGATC502.7937147E-547.492117
GTGTACA205.370722E-447.4921112-13
GTATATG405.346657E-447.492111
TGTGTAC205.370722E-447.4921112-13
GGCTAAT502.7937147E-547.492117
AAAGTAC707.7439836E-847.4921073
TTTATTC707.7439836E-847.4921073
GTGAGAG652.5463742E-643.838871
TATTCTT554.9051057E-543.174645
GGGATCA554.9051057E-543.174648
TCTTTGT459.5512526E-442.2152068
Produced by FastQC (version 0.11.5)